• 제목/요약/키워드: development of egg and larvae

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한국산 벗굴, Ostrea denselamellosa의 유생발생 (The Development of Larvae and Egg of Flat oyster, Ostrea denselamellosa in Korea)

  • 양문호;한창희;김형섭;최상덕
    • 한국패류학회지
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    • 제15권2호
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    • pp.115-119
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    • 1999
  • We investigated the processes of egg and larval developments for aquaculture technique development of seedling production fo the flat oyster, Ostrea denselamellosa. Teo flat oyster of larviparous type was different from the pacific oyser (ovivarous type) because their larvae (trochophore and prodissoconch larvae) in the gill released into the seawater. The process of egg development was observed by artificial fertilization at $25^{\circ}C$, using a dissecting method. The sizes of Unfertilized eggs ranged from 80 to 90 $\mu\textrm{m}$ and fertilized eggs with globule-shape was 90-100 $\mu\textrm{m}$. The Polar body appeared after fertilization and egg cleavage began within 1 hour, reaching the blastula stage after 10 hours. The trochophore in the gill appeared 2-3 days after fertilization and grew to the prodissoconch larvae (130 140 $\mu\textrm{m}$) having a complete shell after 1-2 days. The shell of prodissoconch larvae grew to 205 220 $\mu\textrm{m}$ after 10 hours, and then they became umbo stage larvae showing oval in shape. The velum of umbo stage larvae was degenerated about 17-20 days after fertilization and grew into a pediveliger with a developed foot, at this time, the shell length size was 320 360 $\mu\textrm{m}$.

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난발생중 원유의 수용성 성분에 노출된 해산 양식어류 수정란의 부화율 및 자어의 생존능력 (Hatching Rate and Larval Viability of Cultured Marine Fish Exposed to Water-soluble Fraction of Kuwait Crude Oil during Egg Development)

  • 이갑현;장영진;강덕영
    • 한국양식학회지
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    • 제12권2호
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    • pp.115-122
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    • 1999
  • Exposure experiments during the egg development were conducted to assess the influences of 5 different concentrations (0, 25, 50, 75 and 100%) of water-soluble fraction (WSF) of Kuwait crude oil on the eggs and larvae of black seabream (Acanthopagrus schlegeli), red seabream (Pagrus major) and olive flounder (Paralichthys olivaceus). All experiments were triplicated. Hatching time and hatching rate were examined on the eggs. The median lethal time ($LT_{50}$), morphological abnormality and swimming activity (swimming frequency and speed) of larvae were also investigated. The time and rate of egg hatching were not significantly influenced by WSF on the eggs of the fishes. The larvae exposed to WSF during the egg development were also not significantly influenced on the $LT_{50}$ and swimming activity. But the higher morphological abnormalities of notochord were observed from the larvae in 100% WSF exposure.

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한국산 참마자(Hemibarbus longirostris) (Cypriniformes : Cyprinidae)의 난 발생 및 자치어 형태 발달 (Morphological Development of Eggs, Larvae and Juveniles of the Hemibarbus longirostris (Cypriniformes : Cyprinidae) from Korea)

  • 문성준;박재민;한경호
    • 한국수산과학회지
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    • 제53권2호
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    • pp.203-208
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    • 2020
  • This study was carried out to clarify the larvae and juveniles of egg development and autonomous development of Hemibarbus longirostris from Korea, and to obtain basic data for species conservation and seed production. The shape of the egg was circular and sticky. The average size of the eggs was 2.01 mm (n=10). At 185 hours after fertilization, more than 50% of the total embryos were hatched. The newly hatched larvae had an average total length of 8.10 mm (n=5) and had egg yolk in the abdomen. At 3 days after hatching, the larvae absorbed all egg yolk was average total length 8.64 mm. On the 6 days after hatching, the caudal tip of the notochord started to curve upwards was average total length 10.9 mm. At 70 days after hatching, the average total length 37.9 mm. The number of fins was i 8-10 in dorsal fin, iii 7 in the anal fin, and ii 5 in the ventral fin.

미꾸리 Misgrunus anguillicaudatus (Cypriniformes: Cobitidae)의 난발생 및 자치어 형태발달 (Morphological Development of Eggs, Larvae and Juveniles of the Misgrunus anguillicaudatus (Cypriniformes: Cobitidae))

  • 박재민;유동재;손준혁;한경호
    • 한국수산과학회지
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    • 제55권1호
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    • pp.23-29
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    • 2022
  • This study was carried out to clarify the egg, larvae and juveniles development of Misgurnus anguillicaudatus, and relationships of M. anguillicaudatus and M. mizolepis, Cobitididae Fishes. The adult fishes were collected in Samsan-cheon, Haenam-gun, Jeollanam-do, Korea and their spawning inducement was carried by ovaprim injections. The egg shape was circular and the size was average 1.12 mm. The eggs were hatched at 61 to 72 h after fertilization. The newly hatched larvae had an average 3.23 mm in total length (TL). At 5 days after hatching, the larvae reached to post larval stage and they were 10.3 mm in TL. At 19 days after hatching, it reached to juvenile stage and was 25.3 mm in TL. The egg size of M. anguillicaudatus was almost same as M. mizolepis but the hatching period of M. anguillicaudatus has taken longer. It was possible for interspecific distinguishability of M. anguillicaudatus and M. mizolepis when their larvae reached to juvenile stage by the development of keel-like ridges.

한국 남해안의 멸치(Engraulis japornicus) 난자치어 수송에 관한 연구 (A Study on the Transport of Anchovy Engraulis japornicus Egg-larvae in the South Sea of Korea)

  • 김동선;배상완
    • 한국환경과학회지
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    • 제20권11호
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    • pp.1403-1415
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    • 2011
  • To understand the transport of anchovy egg-larvae, an integrated model consisting of a hydrodynamic model and a three-dimensional Lagrangian diffusion model was used for the anchovy Engraulis japornicus egg-larvae trace. Fist, in order to determine the circulation characteristics of the South Sea of Korea, residual flow according to tide, density and wind effect was investigated. In offshore regions, tide-induced residual current tends to flow eastward during the spring tide and westward during the neap tide. Residual flow is irregular due to the bottom topography in the coastal area. No special tendency was apparent in the open sea. Especially, the flow in the offshore regions showed results similar to that of the Tsushima Warm Current. The transport of anchovy egg-larvae is decided the physical properties of sea water. It is estimated that anchovy eggs spawned near the Jeju Island travels offshore, rather than to coastal areas, and grow in the front area between the coastal sea and offshore regions.

낙동강 수계 위천에 서식하는 큰납지리(Acheilognathus macropterus) (Pisces : Cyprinidae)의 초기생활사 (Early Life History of Acheilognathus macropterus (Pisces:Cyprinidae) in the Wicheon Stream of Nakdonggang River)

  • 박재민;한경호
    • 한국수산과학회지
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    • 제56권5호
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    • pp.676-683
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    • 2023
  • The development of the egg, larvae, and juveniles of Acheilognathus macropterus from the Wicheon stream of Nakdonggang River was investigated for early life history characteristics. The fish used in the study were collected from Wicheon stream located in Bian-myeon, Uiseong-gun, Gyeongsangbuk-do. The fertilized egg was 1.88±0.09 mm (n=30) in long diameter and 1.34±0.06 mm (n=30) in short diameter. After fertilization at a water temperature of 20℃, the egg took 31 h and 40 min to hatch. Newly hatched larvae had an average total length of 3.92±0.13 mm (n=30) and possessed a yolk sac the mouth and anus were not developed. Twenty days after hatching, the postflexion larvae had an average total length of 8.08±0.29 mm (n=30), with the tail tip fully bent at 45° and began feeding. Thirty-three days after hatching, the number of fin stems reached an integer with an average total length of 15.0±1.08 mm (n=30). In this study, differences in the egg size, hatching time, melanophore development period, and distribution location were confirmed between A. macropterus and allied species.

Embryonic and Morphological Development of Larvae and Juvenile of the Buenos Aires Tetra, Hyphessobrycon anisitsi (Pisces Characidae) Characidae Fishes

  • Park, Jae-Min;Han, Kyeong-Ho;Han, Ran
    • 한국발생생물학회지:발생과생식
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    • 제19권1호
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    • pp.25-31
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    • 2015
  • We have launched an investigation for Embryonic Development, Larvae and Juvenile Morphology, of Buenos aires tetra in order to build basic data of Characidae and fish seeding production. We brought 50 couples of Characidae from Bizidduck aquarium in Yeosu-si, Jeollanamdo, from Korea on March of 2015. We put them in the tetragonal glass aquarium ($50{\times}50{\times}30cm$). Breeding water temperature was $27.5{\sim}28.5^{\circ}C$ (mean $28.0{\pm}0.05^{\circ}C$) and being maintained. The shape of fertilized egg was round shape, and it was adhesive demersal egg. The egg size was 0.63~0.91 mm (mean $0.74{\pm}0.07mm$, n=20). After getting fertilized egg, the developmental stage was gastrula stage, and embryo covered almost two-thirds of Yolk. Incubation was happened after 16 hours 13 minutes from gastrula stage, and the tail of juvenile came out first with tearing egg capsule. Immediately after the incubation, prelarvae had 3.78~3.88 mm length (mean $3.84{\pm}0.04mm$, n=5), and it had no mouth and anus yet. 34 days after hatching from the incubation, juvenile had 8.63~13.1 mm (mean $10.9{\pm}1.66mm$), and it had similar silver-colored body shape with its mother.

Morphological Development of Egg, Larvae and Juvenile in Korean shinner, Coreoleuciscus splendidus from the Ungcheon-Stream of Korea

  • Park, Jae-Min;Cho, Seong-Jang;Jo, Hye-In;Han, Kyeong-Ho
    • 한국발생생물학회지:발생과생식
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    • 제23권2호
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    • pp.161-170
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    • 2019
  • The purpose of this study is to investigate the development of Coreoleuciscus splendidus egg and larvae morphology in the Ungcheon-stream. C. splendidus eggs were round and ranged in size from 1.86-2.01 mm (mean $1.91{\pm}0.14mm$). Immediately after hatching, the larvae had egg yolk in the abdomen with a total length of 5.27-6.63 mm (mean $5.95{\pm}0.96mm$). On the 10 days after hatching, the latter was 8.44-8.65 mm (mean $8.54{\pm}0.14mm$) in total length, and 5 dorsal fin rays were formed in dorsal fin and 8 caudal fin soft rays were formed in caudal fin. At 88 days after hatching, total length was 26.2-25.7 mm (mean $25.9{\pm}0.35mm$), and scales were formed throughout the body. The fin rays of each part were iii.7 in dorsal fin and iii.6 in anal fin, and finally the external form was the same as the adult fish.

감성돔, Acanthopagrus schlegeli 수정란과 부화자어의 발달에 미치는 3,5,3'-triiodo-L-thyronine (T3)의 침지처리 효과 (Effects of 3,5,3'-triiodo-L-thyronine (T3) Immersion on Development of Fertilized Egg and Newly Hatching Larva of Black Seabream, Acanthopagrus schlegeli)

  • 강덕영
    • 생명과학회지
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    • 제13권1호
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    • pp.34-41
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    • 2003
  • The hatching of fertilized egg and early development of larval black seabream, Acanthopngrus schlegeli by immersion of 3,5,3'-triiodo-L-thyronine ($T_3$) with doses of 0.01~0.5 ppm in tank were examined for early development periods and compared with control fish immersed by 0.1 N NaOH and 99% alcohol. Larvae were fed with rotifers for first 5 days during experimental period. Yolk absorption of larvae from $T_3$ immersion groups were significantly faster than that of control. Although hatching times among each groups were not significant difference. the hatching rates of larvae in higher doses (0.1 ppm and 0.5 ppm $T_3$ groups) were significantly lower than control. The results of $T_3$ immersion experiments to newly hatching larvae indicated that exogenous $T_3$induced significant faster the absorption of yolk and oil globule, faster the development of myotome and digestive duct and higher the growth rate of larvae than larvae of control. These results indicate that $T_3$ immersion supplements appear to confer a distinct advantage to eggs and larvae of black seabream, which is in early fragile developmental stage.

Egg Development of the Ussurian Bullhead Fish, Leiocassis ussuriensis (Pisces: Bagridae) and Morphological Development of Its Larvae and Juveniles

  • Park, Jae-Min;Yim, Hu-Sun;Lee, Yong-Sik;Kim, Heung-Yun;Han, Kyeong-Ho
    • 한국발생생물학회지:발생과생식
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    • 제19권4호
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    • pp.189-196
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    • 2015
  • This study was examined the ovogenesis of Ussurian bullhead, Leiocassis ussuriensis and the morphological development of its larvae and juveniles and to use the results as basic information for the preservation of species and resource enhancement. For artificial egg collection, human chorionic gonadotropin (HCG) was injected at a rate of 10 IU per gram of fish weight. During breeding period, water temperature maintained at $24.5{\sim}26.5^{\circ}C$ (mean $25.0{\pm}0.05^{\circ}C$). The process of ovogenesis reached the two-cell stage in 50 minutes after fertilization. In 73 hours of fertilization the movement of the embryoid body became active state and the larvae began to hatch from the tail through the oolemma. Length of prelarvae were 6.33~6.50 mm long (mean $6.40{\pm}0.06mm$) just after hatching having yolk with their mouth not opened. After thirty eight days of hatching, juveniles were 30.6~32.5 mm long (mean $31.5{\pm}0.65mm$). The color was dark yellowish brown throughout the entire body, and the number of caudal fin rays developed to thirty six perfectly.