• Proceedings of the Korean Magnetic Resonance Society Conference
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• 2002.08a
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• pp.80-80
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• 2002

MnPSO-34 and Mn-impregnated SAPO-34 (Mn-SAPO-34) sample were prepared with various manganese contents and studied by electron spin resonance (ESR) and electron spin-echo modulation (ESEM). Electron spin-echo modulation analysis of 0.07mol ％ Mn(relative to p) in MnAPSO-34 with adsorbed D$_2$O shows two deuteriums at 0.25 nm and two at 0.36 nm from Mn. This suggests that two waters hydrate an MnO4 configuration with a D-O bond orientation for the waters as expect for a negatively charged site at low manganese content (0.07 mol％), the ESR spectra of MnAPSO-34 and MnH-SAPO-34 exhibit the same parameters (g 2.02 and A 87 G), but the spectra obtained from MnAPSO-34 samples are better resolved. TGA of as-synthesized MnAPSO-34 shows that the decomposition temperature in the range 200-$600^{\circ}C$ of the morpholine is 12$^{\circ}C$ higher than that in as-synthesized MnH-SAPO-34. Infrared spectra shows that the position of a band at about 15 cm-1 toward higher energy in MnAPSO-34 versus MnH-SAPO-34. The modulation depth of the two-pulse ESE of MnAPSO-34 with absorbed D$_2$O is deeper than that of MnH-SAPO-34 with absorbed D$_2$O. Three-pulse ESEM of MnAPSO-34 and MnH-SAPO-34 with absorbed deuterium oxide shows that the local environments of manganese in the hydrated samples are different, suggesting that Mn(II) is framework substituted in MnAPSO-34 since it obviously occupies an extra-framework position in MnH-SAPO-34

• Nuclear Engineering and Technology
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• v.27 no.2
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• pp.226-233
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• 1995

The wall thickness of a pressure tube is increased in order to reduce the probability of failure in a pressure tube of CANDU type reactor. It is presented here that the variation of wall thickness changes stress, hydrogen concentration and delayed hydride cracking in Zr-2.5Nb pressure tube. When the wall thickness is increased from 4.2 mm to 5.2 mm, the stress exerted on the tube and the deuterium taken up during operation are reduced by 19％. Further, the calculated allowable depth of the surface flaw over which delayed hydride cracking(DHC) is susceptible increases by 50％. DHC initiation is controlled by the stress and by the hydrogen concentration in the pressure tube. The results are therefore very significant in such a respect that increased wall thickness may reduce DHC initiation. Ac the wall thickness increases the hydrostatic tension will increase. Its impact on the acceleration of the crack growth rate of DHC deserves further studies.

• Journal of Soil and Groundwater Environment
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• v.17 no.4
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• pp.51-62
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• 2012

Effects of climate change on groundwater system requires understanding the groundwater system in temporal and spatial scales through the long-term monitoring. In this study, the spatio-temporal variations of groundwater were analyzed through the continuous observation of water level, electrical conductivity (EC) and water temperature with automatic data-loggers and sampling in a Gwangneung catchment, Korea, for the four years from 2008 to 2011. Groundwater monitoring were performed at the nest-type wells, MW1 and MW2, located in upsteam and downstream of the catchment, respectively. During the survey period, both the total amount of annual precipitation and the frequency of concentrated rainfall have increased resulting in the elevation of runoff. Water level of MW1 showed no significant fluctuations even during the rainy season, indicating the confined groundwater system. In contrast, that of MW2 showed clear seasonal changes, indicating the unconfined system. The lag-time of temperature at both wells ranged from one to three months depending on the screened depths. Results of chemical analyses indicated that major water compositions were maintained constantly, except for the EC decreases due to the dilution effect. Values of the stable-isotope ratios for oxygen and deuterium were higher at MW2 than MW1, implying the confined system at the upstream area could be locally developed.

• Journal of Biomedical and Translational Research
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• v.19 no.4
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• pp.130-139
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• 2018

This study aimed to analyze a high-performance liquid chromatography (HPLC) separation using a pentafluorophenyl column of parent drug hydroxychloroquine (HCQ) and its active metabolite, desethylhydroxchloroquine (DHCQ) applying to determine bioequivalence of two different formulations administered to patients. A rapid, simple, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for bioanalysis of HCQ and its metabolite DHCQ in human whole blood using deuterium derivative $hydroxychloroquine-D_4$ as an internal standard (IS). A triple-quadrupole mass spectrometer was operated using electrospray ionization in multiple reaction monitoring (MRM) mode. Sample preparation involves a two-step precipitation of protein techniques. The removed protein blood samples were chromatographed on a pentafluorophenyl (PFP) column ($50mm{\times}4.6mm$, $2.6{\mu}m$) with a mobile phase (ammonium formate solution containing dilute formic acid) in an isocratic mode at a flow rate of 0.45 mL/min. The standard curves were found to be linear in the range of 2 - 500 ng/mL for HCQ; 2 - 2,000 ng/mL for DHCQ in spite of lacking a highly sensitive MS spectrometry system. Results of intra- and inter-day precision and accuracy were within acceptable limits. A run time of 2.2 min for HCQ and 2.03 min for DHCQ in blood sample facilitated the analysis of more than 300 human whole blood samples per day. Taken together, we concluded that the assay developed herein represents a highly qualified technology for the quantification of HCQ in human whole blood for a parallel design bioequivalence study in a healthy male.

• Membrane Journal
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• v.34 no.2
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• pp.114-123
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• 2024

Hydrogen isotopes can be categorized into light hydrogen, heavy hydrogen, and tritium based on the number of neutrons, each of which is used in specific fields. Specifically, deuterium is of interest in the electronics industry, nuclear energy industry, analytical technology industry, pharmaceutical industry, and telecommunications industry. Conventional methods such as cold distillation, thermal cycling absorption processes, Girdler sulfide processes, and water electrolysis have their own advantages and disadvantages, leading to the need for alternative technologies with high separation and energy efficiency. In this context, membrane-based hydrogen isotope separation is one of the promising solutions to reduce energy consumption. In this review, we will present the state-of-the-art in hydrogen isotope separation using membranes and their operating principles. The technology for separating hydrogen isotopes using membranes is just beginning to be conceptualized, and many challenges remain to be overcome. However, if achieved, the economic benefits are expected to be significant. We will discuss future research directions for this purpose.

• Journal of Microbiology and Biotechnology
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• v.33 no.12
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• pp.1606-1614
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• 2023

Biochemical gut metabolism of dietary bioactive compounds is of great significance in elucidating health-related issues at the molecular level. In this study, a human gut bacterium cleaving C-C glycosidic bond was screened from puerarin conversion to daidzein, and a new, gram-positive C-glycoside-deglycosylating strain, Dorea sp. MRG-IFC3, was isolated from human fecal sample under anaerobic conditions. Though MRG-IFC3 biotransformed isoflavone C-glycoside, it could not metabolize other C-glycosides, such as vitexin, bergenin, and aloin. As evident from the production of the corresponding aglycons from various 7-O-glucosides, MRG-IFC3 strain also showed 7-O-glycoside cleavage activity; however, flavone 3-O-glucoside icariside II was not metabolized. In addition, for mechanism study, C-glycosyl bond cleavage of puerarin by MRG-IFC3 strain was performed in D₂O GAM medium. The complete deuterium enrichment on C-8 position of daidzein was confirmed by ¹H NMR spectroscopy, and the result clearly proved for the first time that daidzein is produced from puerarin. Two possible reaction intermediates, the quinoids and 8-dehydrodaidzein anion, were proposed for the production of daidzein-8d. These results will provide the basis for the mechanism study of stable C-glycosidic bond cleavage at the molecular level.

• Journal of the Korean Society for Nondestructive Testing
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• v.23 no.4
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• pp.334-341
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• 2003

When the pressure tubes(f are in contact with the calandria tube(CT) in the pressurized heavy water reactor(PHWR), the temperature difference between inner and outer wall of W results in a thermal diffusion of hydrogen (deuterium) and hydride blisters are formed on the outer surface of PT. Because the hydride blisters and zirconium matrix are acoustically continuous, it is not easy to distinguish the blisters from the matrix with conventional ultrasonic method. An ultrasonic velocity ratio method was developed to detect small hydride blisters on the zirconium pressure tube. Hydride blisters were grown in the PT specimen using a steady state thermal diffusion device. The flight times of longitudinal echo and reflected shear echo from the outer surface were measured accurately. The velocity ratio of the longitudinal wave to the shear wave was calculated and displayed using contour plot. Compared to the conventional flight time method of longitudinal wave, the velocity ratio method shows superior sensitivity to detect smaller blisters as well as better images for the blister shapes. Detectable limit of the outer shape of the hydride blisters was conservatively estimated as $500{\mu}m$, with the same specifications of ultrasonic transducer used in the actual PHWR pressure tube inspection.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.38 no.1
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• pp.33-41
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• 2012

An effective, environmentally friendly analytic methods using gas chromatography with mass spectrometric detector (GC-MSD) have been developed for the quantitative analysis of trace phthalate levels in cosmetics such as nail lacquer and hair spray. Since such cosmetics are largely comprised of organic solvents, conventional clean-up methods that have been widely used for phthalate analyses are in adequate. In addition, analysis of trace phthalate levels is notorious for its sensitivity to contamination, which causes high analytical values. A direct sample dilution method using an organic solvent was adopted to the sample preparation process to determine the exact amounts of phthalates and simultaneously avoid the high risk of secondary contamination. The method has many advantages including high accuracy, sensitivity, and simplicity in sample preparation. Dibutyl phthalate (DBP) and di (2-ethylhexyl) phthalate (DEHP) were selected for analysis because they have been frequently detected in cosmetics and consistently reported as endocrine disruptors in humans and animals. Internal standard method using two deuterium substitutes (DBP-$d_4$, DEHP-$d_4$) as the internal standard was also used. The results of 'Method validation' showed the capabilities of this method for the routine analysis of phthalates at the ppm level. The recovery ranges were between 95 % and 106.1 %, and relative standards deviations (RSD) were less than 3.9 % in fortified nail lacquer and hair spray samples at the concentration of $25{\mu}g/g$.

• Journal of Hydrogen and New Energy
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• v.9 no.2
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• pp.77-84
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• 1998

Hydrogen isotope exchange in mixtures of $H_2O/D_2$, $H_2O/D_2O$, or $D_2O/H_2$ can be facilitated under electrical discharge. For example, a simple DC corona discharge through the mixture creates a plasma in which the reactants are excited energetically. The reactants in such plasma, due to increase in population of excited quantum levels or due to production of radicals or ions, undergo very rapid chemical reactions even at ambient temperature. The isotope exchange reaction of hydrogen(H) and deuterium(D) produces the third kind of heavy water(HDO) and isotopic hydrogen gas(HD), as shown in $D_2+H_2O{\rightarrow}HD$ K=11.257(at $25^{\circ}C$) The reaction products can be detected with temporal resolution using the Fourier transform infrared(FTIR) absorption spectroscopy. Since $H_2O$, $D_2O$ and HDO are all infrared active with different absorption peaks, FTIR proves to be a useful tool for monitoring the reaction. Experimental results show that the electrical method is indeed a useful means to promote the reaction, showing a better efficiency than traditional catalytic methods.

• Journal of the Korean Society of Groundwater Environment
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• v.5 no.4
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• pp.192-202
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• 1998

We investigated the geochemistry and environmental isotopes of granite-bedrock groundwater in the Yeongcheon diversion tunnel which is located about 300 m below the land surface. The hydrochemistry of groundwaters belongs to the Ca-HCO$_3$type, and is controlled by flow systems and water-rock interaction in the flow conduits (fractures). The deuterium and oxygen-18 data are clustered along the meteoric water line, indicating that the groundwater are commonly of meteoric water origin and are not affected by secondary isotope effects such as evaporation and isotope exchange. Tritium data show that the groundwaters were mostly recharged before pre-thermonuclear period and have been mixed with younger surface water flowing down rapidly into the tunnel along fractured zones. Based on the mass balance and reaction simulation approaches, using both the hydrochemistry of groundwater and the secondary mineralogy of fracture-filling materials, we have modeled the low-temperature hydrogeochemical evolution of groundwater in the area. The results of geochemical simulation show that the concentrations of Ca$\^$2＋/, Na$\^$＋/ and HCO$_3$and pH of waters increase progressively owing to the dissolution of reactive minerals in flow paths. The concentrations of Mg$\^$2＋/ and K$\^$＋/ frist increase with the dissolution, but later decrease when montmorillonite and illitic material are precipitated respectively. The continuous adding of reactive minerals, namely the progressively larger degrees of water/rock interaction, causes the formation of secondary minerals with the following sequence: first hematite, then gibbsite, then kaolinite, then montmorillonite, then illtic material, and finally microcline. During the simulation all the gibbsite is consumed, kaolinite precipitates and then the continuous reaction converts the kaolinite to montmorillonite and illitic material. The reaction simulation results agree well with the observed, water chemistry and secondary mineralogy, indicating the successful applicability of this simulation technique to delineate the complex hydrogeochemistry of bedrock groundwaters.