• Asian-Australasian Journal of Animal Sciences
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• 제9권2호
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• pp.203-210
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• 1996

A 13-month study was conducted to determine the monthly variation of crude protein(CP), cell contents(CC), fiber fractions and mineral concentrations of paragrass [Brachiaria mutica (Forsk.) Stapf.] and stargrass (Cynodon plectostachyum Pilger) and to estimate the correlations among the nutrient fractions and climatic factors. The forage samples collected by hand plucking were found to contain CP and mineral concentrations. i.e. calcium(Ca), magnesium(Mg), potassium(K) and zinc(Zn) above the critical levels based on ruminant veeds. Monthly differences(p<0.05) were observed in all CP, CC, neutral detergent fiber(NDF), acid detergent fiber(ADF), hemicellulose(HE), cellulose(CE) and acid detergent lignin(ADL) between the two grass species. Monthly differences(p<0.05) were also observed in all concentrations of forage Ca, K, phosphorus(P), copper(Cu) and Zn except in Mg of both grasses and K of stargrass. Species differences(p<0.05) were observed in all nutrient fractions except Mg and K concentrations. Rainfall had positive correlations to CP, P(p<0.01) and CC(p<0.05); it had negative correlations to NDF(p<0.05). ADF, CE, Ca, Cu and Zn(p<0.01). Temperature, humidity and daylength had also some correlations to various nutrient fractions.

• 한국식품영양과학회지
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• 제15권4호
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• pp.51-55
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• 1986

본 계량적 분체 낙하봉지는 현재 사용하고 있는 4각형 합성수지제 분체 포장의 구조를 개조하여 최종 용기로써 계량적 사용이 가능 하도록 봉지 몸체에 분체 박하유도 튜브를 연결하고 그 튜브 끝단에 개폐용 핀을 채운 구조이다. 이 계량적 분체 박하봉지는 현재 사용하고 있는 분체 봉지에 비해서 봉지의 개봉이 쉽고, 분획 사용 후 재 밀봉이 긴밀하게 될 수 있으며, 재 밀봉이 철저하게 되므로 흡방지 및 anti-caking효과가 크다. 그리고 먼지나 곤충과 같은 이물질의 혼입이 없으며 계량적 사용으로 경제적이고 환경과 하수의 오염을 최소화 하는 효과가 있다. 또한 개봉후 세워둘 때 넘어져 엎지러져 일어나는 손실이 없는 등의 장점이 있다. 따라서 본계량적 분체 낙하봉지는 분상의 설탕, 소금, MSG와 같은 조미료, coffee 및 세제는 물론 농약의 계량적 사용에 실용성을 줄 것으로 생각된다.

• 한국잠사곤충학회지
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• 제13권1호
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• pp.69-72
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• 1971

1. 연감시료량의 적정량을 알아보기 위해 200, 100, 50, 20, 10gr의 생사시료를 연감검사한 결과 각처리간에는 통계적차이를 인정할 수 없었으나 실제로 시료량의 과대 과소에 따른 측정 및 처리오차를 감안할 때 100gr 시료가 적정량이었다. 2. 섬도사를 이용하여 연감검사를 할 경우 꼬인 상태와 풀은 상태로 연감처리할 경우 탄산나트륨같이 제교력이 강한 정연제에서는 차이가 없었으나 Marseilles 비누같이 제교력이 약한 것은 연감율에 있서 현저한 차이가 있었다. 3. 5종의 정연제를 비교한 결과 합성세제와 국산 Marseilles비누와 통계적 유의차를 인정 할 수 없으므로 이와의 대체 이용이 가능하다. 4. 정연제의 제교력은 대체로 정연액의 PH가 클수록 크고 작을수록 작은 경향이 있다.

• 물과 미래
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• 제27권1호
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• pp.69-77
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• 1994

생활수준이 향상됨에 따라 세제의 사용량이 큰 폭으로 증가하고 있으며, 하수처리공정에서 완전히 처리되지 못한 합성세제가 하천이나 강으로 흘러들어 지표수의 오염을 가중시키고 있다. 이와같은 세제는 수중생물에 해로운 여러 독성물질을 함유하고 있으며, 현재까지는 주로 화학적 분석에 의하여 그 독성을 측정하고 있다. 그러나 오염물질 상호간의 작용 등으로 화학분석만으로 적정기간동안에 독성을 평가하는 것은 상당히 어려운 실정인 바, 본 논문에서는 물벼룩인 Daphnia magna와 형광성 박테리아인 Photobacterium phosphoreum을 이용하여 생물학적으로 주방용 세제와 의류용 세제의 급성 독성을 평가하였다. 본 평가에 사용된 세제는 세탁용 세제와 주방용 세제로 구분하여 국내 사용량이 가장 많은 7종류를 선택하였으며, 조사결과 세탁용 세제의 평균 24hr, 48hr-LC50은 각각 4.25%, 2.50%이며, 주방용 세제의 평균 24hr, 48hr-LC50은 각각 2.01%, 1.36%이었다. 또한 세탁용 세제의 평균 5min, 15min-EC50은 각각 1.83%, 1.02%이며, 주방용 세제의 평균 5min, 15min-EC50은 각각 1.57%, 1.21% 이었다. 이를 통하여 국내에서 사용되는 합성세제중 주방용 세제가 세탁용 세제보다 지속적 독성이 큰 것을 알 수 있었으며 세제마다 독성의 정도가 차이가 있었다.

• 한국초지조사료학회지
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• 제11권1호
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• pp.60-67
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• 1991

This experiment was conducted to investigate the effects of starch addition on the fermentative quality and dry matter digestibility(DMD) of Kudzu(Puerari~ thzmbergii Bentham) silage. The herbages was ensiled by the conventional methods in small plastic silo of 7.5 liters with addition of starch of 0 %, 2 %, 4 74, 6 % and 8 %, respectivery. The samples of kudzu silage were separated into leaf and stem, and was determined the pH, organic acid and characteristics of fiber such as neutral detergent fiber(NDF), acid detergent liber(ADF) and acid detergent lignin(ADL). The DMD of leaf and stem silage were evaluated by pepsin-cellulase technique method. The energy values(tota1 digestible nutrients, TDN; digestible energy, DE; metaboliz;~bie energy. ME) were calculated by DMD. The results obtained were as follows: 1. The fiberous meterials(such as NDF, AIIF and ADL) of leaf and stem were decreased with increasing levels of starch(p

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권1호
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• pp.19-25
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• 2006

With particular regards to the hepatitis A virus (HAV), a terminal dry-heat treatment ($100^{\circ}C$ for 30 min) process, following lyophilization, was developed to improve the virus safety of a solvent/detergent-treated antihemophilic factor IX concentrate. The loss of factor IX activity during dry-heat treatment was of about 3%, as estimated by a clotting assay. No substantial changes were observed in the physical and biochemical characteristics of the dry-heat-treated factor IX compared with those of the factor IX before dry-heat treatment. The dry-heat-treated factor IX was stable for up to 24 months at $4^{\circ}C$, The dry-heat treatment after lyophilization was an effective process for inactivating viruses. The HAV and murine encephalomyocarditis virus (EMCV) were completely inactivated to below detectable levels within 10 min of the dry-heat treatment. Porcine parvovirus (PPV) and bovine herpes virus (BHV) were potentially sensitive to the treatment. The log reduction factors achieved during lyophilization and dry-heat treatment were ${\ge}5.60$ for HAV, ${\ge}6.08$ for EMCV, 2.64 for PPV, and 3.59 for BHV. These results indicate that dry-heat treatment improves the virus safety of factor IX concentrates, without destroying the activity. Moreover, the treatment represents an effective measure for the inactivation of non-lipid enveloped viruses, in particular HAV, which is resistant to solvent/detergent treatment.

• 멤브레인
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• 제9권4호
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• pp.202-208
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• 1999

오일과 세척제가 다량함유되어 있는 자동차 세척배수를 한외여과막으로 처리하였다 세차배수 처리에 적절한 분리막과 투과현상을 평가하기 위하여 분획분자량이 10, 30 및 100k dalton인 한회여과막과 dead-end 방식의 stirred cell (Amicon 8050)을 사용하여 투과유고과 제거율을 측정하였다 분획분자량이 작은 막에 경우 막오염현상이 미약하였으나 분획분자량이 큰 YM100 (100k dalton)인 경우에는 분리막 표면에 오일층을 형성 뿐만 아니라 막 세공 중 일부는 운전압력 조건에 따라서 변형이 가능한 오일 입자가 막을 가능성이 있다 그러나 오일 및 입자 배제율 95% 이상이며 세척제를 포함한 투과수를 재활용할 수 있어 수질오염을 최소화할 수 있을 것으로 판단된다. 또한 분획분자량이 50k dalton인 중공사형 한외여과막을 이용한 세차배수의 연속적 처리실험을 하였으며 케이크 여과모델이 잘 적용굄을 확인할수하였다.

• 한국환경교육학회지:환경교육
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• 제8권1호
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• pp.66-80
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• 1995

The purpose of this study was to investigate the housewives's recognition and practice on prevention of environmental pollution. The subjects of this study were 250 housewives, school students' mothers, living in Cheong-ju. Major findings were as follows: 1. In washing their faces and brushing their teeth, they saved the water very well, but in taking bath, washing the dished, using the water of the lavatory they did not save the water so well. 2. The kinds of the cleansers differed in taking a bath, washing the hair, and doing the laundry respectively : what they use most was hard soaps in taking bath, liquid cleansers mixed with shampoo and linse in washing the hair, and synthetic powder detergent in laundrying. They used more synthetic detergent than natural soaps, which is known to be a cause of water pollution. Especially, when they cleansed, they did not use a measuring cup. It resulted in the waste of detergent and accelerating of water pollution. Therefore, the environmental education for them was very urgently needed. 3. In handling domestic waste, the separate collection rate of empty bottles and old newspapers was very high, but that of used phone-call cards and used batteries was extremely low. It was truly nessesary to educate and step up publicic activities on the separate collection of phone-call cards and batteries caused environmental pollution. 4. The housewives had much knowledge about environmental pollution, but they did not practice it so well in their home. 5. The housewives made more effort than their children in preventing environmental pollution and saving resources. In conclusion, what is most important for solving environmental problem was for each citizen to make an effort to prevent environmental pollution, and the government's support and producing the atmosphere of the society for this was really needed.

• 한국미생물·생명공학회지
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• 제47권2호
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• pp.259-268
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• 2019

In this study, the extracellular metalloprotease from Serratia marcescens PPB-26 was purified to homogeneity via ethanol fractionation and DEAE-cellulose column chromatography. Thus, a 3.8-fold purification was achieved with a 20% yield and specific activity of 76.2 U/mg. The purified protease was a 50-kDa monomer whose optimum pH and temperature for activity were 7.5 and $30^{\circ}C$ respectively; however, it was found to remain active in the 5-9 pH range and up to $40^{\circ}C$ for 6 h. The protease had a half-life of 15 days at $4^{\circ}C$, an optimum reaction time of 10 min, and an optimum substrate (casein) concentration of 0.25%. Furthermore, the Michaelis constant ($K_m$) and reaction velocity ($V_{max}$) of the protease were calculated to be 0.28% and $111.11{\mu}moles/(min{\cdot}mg)^{-1}$, respectively. The protease was stable when subjected to metal ions (2 mM), showing increased activity with most (especially $CoCl_2$ and $MgSO_4$ (30.54% increase)). It was also stable when exposed to oxidizing agents, bleaching agents, and detergents (5% v/v for 60 min). It retained 93% of its activity in non-ionic detergents (Tween-20, Tween-80, and Triton X-100). Moreover, wash performance analysis in commercial detergents (Ariel and Tide) showed that not only was the protease capable of protein stain removal, but also reduced cleaning time by 80% when added to detergents. Thus, the Serratia marcescens PPB-26 metalloprotease appears to be a promising new candidate as a laundry additive in the detergent industry.

• Asian-Australasian Journal of Animal Sciences
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• 제32권1호
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• pp.82-91
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• 2019

Objective: This experiment was conducted to compare the structure and composition of ruminal microorganisms in goats with high and low neutral detergent fibre (NDF) digestibility. Methods: Nineteen crossbred goats were used as experimental animals and fed the same total mixed rations during the 30-day pre-treatment and 6-day digestion trialperiods. All faeces were collected during the digestion period for measuring the NDF digestibility. Then, high and the low NDF digestibility individuals were chosen for the high NDF digestibility group (HFD) and low NDF digestibility group (LFD), respectively. Rumen contents were collected for total microbial DNA extraction. The V4 region of the bacterial 16S rRNA gene was amplified using universal primers of bacteria and sequenced using high-throughput sequencer. The sequences were mainly analysed by QIIME 1.8.0. Results: A total of 18,694 operational taxonomic units were obtained, within 81.98% belonged to bacteria, 6.64% belonged to archaea and 11.38% was unassigned microorganisms. Bacteroidetes, Firmicutes, and Proteobacteria were the predominant microbial phyla in both groups. At the genus level, the relative abundance of fifteen microorganisms were significantly higher (p<0.05) and six microorganisms were extremely significantly higher (p<0.01) in LFD than HFD. Overall, 176 core shared genera were identified in the two groups. The relative abundance of 2 phyla, 5 classes, 10 orders, 13 families and 15 genera had a negative correlation with NDF digestibility, but only the relative abundance of Pyramidobacter had a positive correlation with NDF digestibility. Conclusion: There were substantial differences in NDF digestibility among the individual goats, and the NDF digestibility had significant correlation with the relative abundance of some ruminal microorganisms.