• Title/Summary/Keyword: detachment

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Effects of Citrus Reticulata on the Cell Detachment and Apoptosis in Human Gastric Cancer SNU-668 Cells

  • Kim, Jeung-Beum;Kim, Min-Su;Kim, Ee-Hwa;Kim, Sung-Hoon
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.19 no.1
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    • pp.212-217
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    • 2005
  • The purpose of this study was to examine the effects of Citrus Reticulata(CR) on the Cell Detachment and Apoptosis in Human Gastric Cancer SNU-668 Cells. The effect of CR on apoptosis was investigated through MTT assay, DAPI staining, and TUNEL assay. We also performed RT-PCR for apoptotic genes including BCL-2, BAX, and caspase-3, the caspase-3 activity assay, and western blotting for pro-CASP-3. Then, to detect that adhesion of cell to ECM was reduced by CR, we investigated mRNA expression of CDH1 and PTK2 using RT-PCR, and their protein expressions using western blotting, and immunocytochemistry in SNU-668 cells. In this study, the results showed that treatment of CR induced time and dose-dependent cell death in SNU-668 cells. Downregulated mRNA expression of BCL-2, and upregulated mRNA expressions of BAX and CASP-3 indicated that the cell death was due to apoptosis. Protein expression of inactivated CASP-3, and caspase-3 activity assay also showed that apoptosis was induced in CR-treated cells.

Role of Detached Particles During Initial Filtration Phase (여과초기에서의 탈착된 입자의 거동)

  • Kim, Ja-Kyum;Tobiason, John E.
    • Journal of Korean Society of Water and Wastewater
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    • v.19 no.1
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    • pp.16-24
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    • 2005
  • Mathematical model was developed to verify a sequential particle removal taking place in a granular media gravity filter. Consequential multi-layer filtration cycle model was applied to verify the fraction of filter effluent particles that are filter influent particles that were never removed as well as the fraction of filter effluent particles that were detached after deposition were performed through laboratory experiments. Three sizes of marker particles were injected ahead of the filter column as a pulse in the presence of four sizes of polystyrene particles that were used as a primary source of particles in the raw suspension to investigate particle attachment alone in contrast to net removal from attachment and detachment. Microscopic counting of filter effluent particles was assumed to reflect attachment. Experimental results indicated that particle detachment is significant beginning from the early phase of filtration. For each size of fluorescent microspheres at one filter depth, fluorescent microsphere removal increased with filter runtime to a maximum due to ripening. The detached fraction of effluent particles increased with particle size and filter depth. The presence of detached particles and the increasing fraction of detached particles in deeper bed were confirmed.

ADHESION PHENOMENON AND ITS APPLICATION TO MANIPULATION FOR MICRO-ASSEBMLY

  • Takahashi, Kunio;Himeno, Hideo;Saito, Shigeki;Onzawa, Tadao
    • Proceedings of the KWS Conference
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    • 2002.10a
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    • pp.781-784
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    • 2002
  • Adhesion phenomenon is more significant for smaller objects, because adhesional force is proportional to size of the objects while gravitational force is proportional to the third power of it. For the purpose of microassembly, theoretical understanding is required for the Adhesion phenomenon. Authors have developed a force measurement system in an ultra-high vacuum chamber of Auger electron spectroscopy. The force between arbitrary combination of materials can be measured at a pressure less than 100 nPa after and before Ar ion sputtering and chemical analysis for several atomic layers of the surface. The results are successfully interpreted with a theory of contact mechanics. Since surface energy is quite important in the interpretation, electronic theory is used to evaluate the surface energy. In the manipulation of small objects, the adhesional force is always attractive. Repulsive force is essential for the manipulation. It can be generated by Coulomb interaction. The voltage required for detachment is theoretically analyzed and the effect of boundary conditions on the detachment is obtained. The possibility and limitations of micro-manipulation using both the adhesion phenomenon and Coulomb interaction are theoretically clarified. Its applicability to nano-technology is found to be expected.

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Seasonal Abundance of Deer and Horse Flies (Diptera: Tabanidae) in the Northern Part of Gyeonggi-do, Republic of Korea

  • Suh, Sang Jae;Kim, Heung Chul;Chong, Sung Tae;Kim, Myung Soon;Klein, Terry A.
    • Parasites, Hosts and Diseases
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    • v.53 no.3
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    • pp.307-314
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    • 2015
  • The seasonal abundance of horse and deer flies (family Tabanidae) was analyzed using Mosquito Magnet$^{(R)}$ traps at 5 sites located near/in the demilitarized zone, northern Gyeonggi-do, Republic of Korea from late April to early October for 4 consecutive years (2010-2013). A total of 2,999 horse and deer flies (tabanids) belonging to 5 genera and 20 species were collected. Chrysops mlokosiewiczi (90.9%) was the most frequently collected, followed by Haematopota koryoensis (4.8%) and C. suavis (1.0%). The remaining 17 species comprised only of 3.3% of all species collected. C. mlokosiewiczi demonstrated bimodal peak populations during mid-June and early August, while H. koryoensis demonstrated a unimodal peak during mid-July. Overall numbers of tabanids collected were influenced by the previous year's winter temperatures and precipitation. Population abundance was influenced by habitat with most of tabanids collected from habitats near forested areas, followed by rice paddies, and a beef farm.

The Effect of Interpersonal Orientation on Consumers' Buying Motivations (대인관계성향이 구매동기에 미치는 영향)

  • Kim Kee-Ok;Yoo Hyun-Jung;Nam Su-Jung
    • Journal of Families and Better Life
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    • v.24 no.3 s.81
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    • pp.83-94
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    • 2006
  • In order to understand how consumers' buying motivation is formed, that is the starting point of consumer behavior or consumers' decision-making processes, we examined how the interpersonal orientation for consumers is organized to control buying motivation and create a stepping-stone for subjects' behavior in fast varied consumer environments. In addition, we examined what influence this interpersonal orientation has on consumers' buying motivations. The results are shown as follows: First, interpersonal orientation is classified into six categories, according to Cohen's three types, i.e., compliance, detachment I: emotion independence, detachment II: performance independence, detachment III: perception independence, aggressiveness I: competitiveness, aggressiveness II: aggressiveness. Second, based on our inspection of the relation between interpersonal orientation and consumers' buying motivations, for external motivation and internal motivation, the value of R2 in regression seems too low to act as an influencing power on interpersonal orientation, while in case of introjected motivation, identified motivation I: self respect, and identified II: pursuit of harmony, I there seems to be some relationship.

Rubber Packing Damage Test Report for Procedure of Forebody Erection in the Sea (해상탑재 DAM공법에 따른 선체외판 선저부위의 도막박리 및 밀림방지 대책)

  • Kim Young-Nam;Im Myung-Soo;Yea Weon-Bae
    • Special Issue of the Society of Naval Architects of Korea
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    • 2005.06a
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    • pp.144-150
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    • 2005
  • The size of ship is being larger than the past in the shipping industry thanks to the increased quantity of goods transported by ship. Therefore, HHIC (Hanjin Heavy industries & Construction co., LTD) invented innovative construction method, so called 'DAM', to build a ship which is longer than the length of the HHIC's dry dock. On Erection at the sea by the application of DAM Method, Squeezing & Detachment of A/F film may occur as a result of the wave and the constriction between A/F film pre-applied on the side shell and DAM's Rubber Packing. Thus, the test for finding the minimum curing time of A/F film was performed to protect Squeezing & Detachment of A/F film on the hull. To verify the soundness of paint and to find the optimum condition during the erection period of the DAM, laboratory test was carried out under no immersion condition through the application of various coatings on the Rubber Packing. And two methods were selected from the results of laboratory test for actual MOCK-UP Test. In addition, the test for the film profile per temperature of silicone A/F coat and the film Squeezing was performed.

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Development of persimmon harvest apparatus -Development of detachment device (감 수확기구 개발(1) - 탈과장치 개발 -)

  • Woo, D.G.;Kim, T.H.
    • Current Research on Agriculture and Life Sciences
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    • v.27
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    • pp.1-6
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    • 2009
  • Persimmon occupied the second largest cultivation area next to apple among the fruits in Korea. Since 70 % of its cultivating field is located at slope, the efficiency of its harvesting operation is very low. Also, the traditional persimmon harvest apparatus does not seem to be efficient to use due to a structural problem. In this paper, the author has analyzed the physical properties of persimmon friut-stem system and compared detachment force with developed persimmon harvest apparatus and traditional persimmon harvest apparatus in order to solve the problems mentioned above. The results of the research are summarized as follows : 1. The weight of the persimmon is shown as 157 g on average, the lengths of stem's major axis and minor axis is shown as 4.6 mm and 3.7 mm on average, respectively, sectional area of stem is shown as $13.9mm^2$ on average and the stem length is shown as 13.6 mm on average. 2 In case of the traditional persimmon harvest apparatus, the detachment force needed when a persimmon was detached from its stem was shown as 86.3 N on average. 3. In case of the developed persimmon harvest apparatus, detachment force needed when a persimmon was detached from its stem was shown as 72.6 N on average.

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Analysis of the particulate matters in the vertical-flow woodchip wetland treating stormwater from paved road (포장도로 강우유출수 처리목적의 수직흐름형 우드칩 충진 습지에서 입자상 물질분석)

  • Yuan, Qingke;Kim, Youngchul
    • Journal of Wetlands Research
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    • v.20 no.2
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    • pp.145-154
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    • 2018
  • In this study, three pilot-scale wetland systems were built for treating stormwater runoff from asphalt road. Each of the system consists of a settling tank and a vertical flow wetland packed with 25%, 50%, and 75% woodchip as treatment media. According to the analysis of the distributions of particle size, it was found that solids ranging in size $0.52-30{\mu}m$ were predominant in the stormwater runoff. After 24-hours settling, those coarser than $20{\mu}m$ were significantly detained. Further retention, especially for the finer-sized fraction, occurred in the wetland through internal recirculation during the dry day periods. As a primary media of the wetland, woodchip showed a high filtration and attachment capacity for the particulates in pre-settled stormwater, whereas overall amount of solids in the wetland effluent increased due to the detachment of woody elements from the media. This was observed mainly during the initial 75 days of operation, and the size and detachment rate were found to be strongly related with the woodchip packing ratio. The mechanism involving woody particle detachment was modeled as a first-order form. In addition, water quality factors and operational parameters affecting the detachment were analyzed and discussed.

Physical Properties or Jujube (Zizyphus jujuba miller) and Jujube Branches (대추 및 대추가지의 물리적 특성)

  • 민경선;이상우;허윤근;서정덕;맹성렬
    • Journal of Biosystems Engineering
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    • v.27 no.4
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    • pp.283-292
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    • 2002
  • Mechanical and physical properties of various parts of jujube (Zizyphus jojoba Miller) such as fruits, leaves, secondary branches, and leafy stems were measured and analyzed. The physical dimensions of the fruits were measured using a digital caliper, and the detachment force of the fruit and leafy stems was measured using a force gauge. The physical characteristics of the secondary branches such as the modulus of elasticity and the torsional rigidity were tested using a universal testing machine (UTM). The physical characteristics of leafy stems such as length and weight were also measured using a digital caliper and a digital scale, respectively. The detachment force of leafy stems and the area of the leaf also measured. The terminal velocities of the jujube fruits, leaves, and leafy stems were measured using a custom made terminal velocity experiment system. Diameter of the major and minor axis of the jujube fruit, weight of the fruit, and detachment force of the fruit stem was average of 32.02 mm, 23.92 mm. 10.0 ${\times}$ 10$\^$6/ ㎥, 8.99 g, and 5.43 N. respectively. The detachment forces of the jujube fruits increased and the force-to-weight ratio of the jujube fruits decreased as the weight of the jujube fruits increased. The modulus of elasticity of the secondary branches of the jujube was average of 7.01 ${\times}$ 10$\^$8/ N/㎡ and decreased as diameter of the secondary branches increased. The average torsional rigidity of the secondary jujube branches was 5.2 ${\times}$ 10$\^$-/ N/㎡, and the torsional rigidity decreased as the secondary branch diameter increased. The distribution of the torsional rigidity data associated with the diameter of the branches, however. widely scattered and it was difficult to find any relationship between the diameter of the branches and the torsional rigidity of tile branches. The weight of the leafy stems, number of loaves attached to the leafy stems, diameter of the stem side of the leafy stems, diameter of the leafy stem end was average or 0.7 g, 6.6 ea, 12.2 cm, 4.5 mm, and 2.7 mm, respectively. The major and minor axis of the .jujube loaves, area of leaves, weight of the leaves. and detachment force of the leaves was average of 5.7 cm, 3.3 cm, 12.98 cm$^2$, 0.20 g, and 4.39 N, respectively. The terminal velocity of the .jujube fruits increased as the weight of the fruits increased. The terminal velocity of the leafy stems, however, did not show a relationship with the weight of the leafy stems and the number of leaves attached to the leafy stem. The terminal velocity, however, slightly increased as the length of the leafy stems increased.

A Case of Suprachoroidal Hemorrhage after Pars Plana Vitrectomy for Rhegmatogenous Retinal Detachment (열공망막박리 환자에서 유리체절제술 후 발생한 맥락막상강출혈 1예)

  • Kim, Min Han;Oh, Jong-Hyun
    • Journal of The Korean Ophthalmological Society
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    • v.59 no.11
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    • pp.1082-1086
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    • 2018
  • Purpose: To report a case of a 60-year-old female with rhegmatogenous retinal detachment, presenting with suprachoroidal hemorrhage after vitrectomy. Case summary: A 60-year-old woman visited our clinic complaining of floaters, flashing, and blurred vision. Best-corrected visual acuity was 0.1 in her left eye, and fundus examination of her left eye revealed macula-involved retinal detachment with a retinal break at the superotemporal quadrant. She underwent cataract surgery, 23-gauge transconjunctival sutureless vitrectomy, and 14% C3F8 gas tamponade under general anesthesia. One hour after anesthesia recovery, she suddenly complained of severe pain in her left eye. The intraocular pressure measured after removal of the pressure patch from her left eye was as high as 58 mmHg. Her ocular pain improved spontaneously within 10 minutes, and the intraocular pressure decreased to 8 mmHg. Fundus examination of her left eye revealed a reddish-brown raised lesion, suggesting suprachoroidal hemorrhage. She was placed in a prone position with a pressure patch over her left eye. Bleeding through the sclerotomy site was observed 1 day after surgery. Subsequently, hemorrhagic choroidal detachment of her left eye continued to decrease without deterioration. Three weeks after surgery, the patient received an intravitreal injection of 100% C3F8 gas into her left eye. At 3 months after surgery, best-corrected visual acuity had improved to 0.8 in her left eye, and the retina was stable. Conclusions: Suprachoroidal hemorrhage may be suspected in a patient complaining of severe ocular pain after vitrectomy.