• Applied Biological Chemistry
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• 제39권6호
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• pp.424-429
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• 1996

유색미 쌀겨가 화학적 변이원에 의한 유전독성을 억제하는 작용기작을 유색미 품종인 수원415호 쌀겨의 유기용매추출물과 변이원인 mitomycin C를 이용하여 조사하였다. 70%에탄올 분획 및 클로로포름분획에서 수원 415호는 대조구로 사용된 추청에 비하여 변이원에 대한 억제활성이 월등히 높았음에도 불구하고 항산화활성은 추청보다 다소 낮은 결과로 보아 항산화작용과는 다른 억제기작이 존재함을 추정할 수 있었다. E. coli를 지시세포로 유색미 쌀겨 추출물의 mitomycin C에 대한 변이원성 억제기작을 조사한 결과, desmutagen으로 작용할 가능성이 크다는 사실을 알았고, 쌀겨 추출물과 mitomycin C를 반응시킨 후 반응액 중에 존재하는 유리상태의 mitomycin C를 정량한 결과, 유색미의 쌀겨 추출물이 mitomycin C에 직접 결합하여 변이원을 흡착함으로써 세포에 대한 유전독성을 억제할 것으로 생각되었다.

• 한국식품영양학회지
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• 제9권3호
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• pp.294-298
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• 1996

By the 505 chromotest which utilized Escherichia bolt PQ 37, Korean medicinal plants had been screened to Investigate the antimutagenic effect to aflatoxin B1(AFBl). Ikmocho(IMC, Leonurus sibiricus L.) was extracted with hot water. The extract was not found to be mutagenic in the Salmonella mutation test with or without metabolic activation, and the extract was showed to possess the antimutagenic properties towards AFB1-induced metation. The mutagenicity of AFB1 was inhibited by methanol soluble fracstion (IMC-MS) in dose-dependent. However, water-soluble fraction exhibited comutagenic activity. The greatest inhibitory effect of IMC-MS on AFB1 mutagenicity occurred when IMC-MS was first incubated, AFB1 followed by a second incubation with the cells and 59 mixture. Also lower inhibition was occurred when S9 mixtures were first incubated, with IMC-MS followed by a second incubation with AFBI. The results of the sequential incubation study support the probability that one mechanism of inhibition could involve the formation of chemical complex between IMC-MS and AFB1 rather than deactivation of S9 enzyme.