• Journal of Environmental Science International
• /
• v.6 no.2
• /
• pp.173-182
• /
• 1997

The bacterial strains, which utilizes 2,4,4'-trichloro-2'-hydroxydiphenyl ether(TCHDPE) as a sole carbon source, were isolated by selective enrichment culture from soil samples of industrial waste deposits. The bacterium that showed the highestt biodegradation activity was designated as EL-O47R The isolated strain EL-O47R was Identified as the genus Pseudomonas from the results of morphological, cultural, and biochemical tests. The optimum conditions of medium for the growth and the degradation of TCHDPE were TCHDPE 500 ppm, (NH4)2SO4 0.1% as the nitrogen source, initial pH 7.0±0.1, and 37℃, respectively. In this conditions, the regradation rate of TCHDPE was about 97%. Pseudomonas sp. EL-O47R was tested for resistance to several metal compounds and antibiotics. Pseudomonas sp. EL-O47R was moderately grown to Cd(NO3)2, ZnCl2, AgSO4, CuSO4 and HgCl2. This strain was sensitive to rifampicin and kanamycln but resistant to ampicillin, penicillin, tetracyclin and chloramphenlcol. Pseudomonas sp. EL-O47R was grown structurally related com- pounds and potential metabolites of TCHDPE, and has the stability on TCHDPE biodegradation.

• Microbiology and Biotechnology Letters
• /
• v.26 no.4
• /
• pp.303-308
• /
• 1998

A bacterial strain which utilizes phenol under denitrifying condition was isolated from the industrial waste water collected from the Chong-ju Industrial Complex. The strain was identified as Pseudomonas species from the morphological, physiological, and biochemical characteristics and designated as HL100. The strain can utilize phenol as the sole source of carbon and energy when nitrate is provided as the terminal electron acceptor. The isolated strain completely degraded 3 mM of phenol within 110 hour with concomitant reduction of nitrate to nitrite. The observed maximum doubling time was 20 hours. Under appropriate condition, complete reduction of nitrate to atmospheric N$_2$ was observed indicating that the isolated strain could perform complete steps of denitrification. The strain showed optimal growth at pH 7.0 and temperature of 37$^{\circ}C$ under denitrifying phenol-degrading condition. The strain can also utilize toluene as the sole carbon and energy source under the same growth condition. However, no growth was detected on xylene and benzene.

• Journal of Life Science
• /
• v.21 no.5
• /
• pp.700-705
• /
• 2011

Using enrichment procedures, we isolated organic solvent-tolerant Bacillus sp. BCNU 5005 from waste water and soil in the Ulsan industrial plant region. BCNU 5005 had a maximum similarity of 98% with B. subtilis and was designated as B. subtilis based on phylogenetic analyses using 16S rDNA sequences. Generally, most bacteria and their enzymes are destroyed or inactivated in the presence of high concentrations of organic solvents. However, the lipase activity of B. subtilis BCNU 5005 was very stable in the presence of various kinds of solvents (25%, v/v) except chloroform, ethylbenzene and decane. Furthermore, BCNU 5005 was determined to have a degradative ability towards organic solvents. This organic solvent tolerant Bacillus sp. BCNU 5005 could be used as a new potential resource for biotransformation and bioremediation.

• Journal of Korea Society of Industrial Information Systems
• /
• v.17 no.7
• /
• pp.17-24
• /
• 2012

As the resources and power on Ad-hoc networks are limited, new data aggregation techniques are required for energy efficiency. The current research on data aggregation techniques is actively in progress, but existing studies don't consider the density of nodes. If nodes are densely placed in a particular area, the information which the sensor nodes placed on those areas detect can be judged as very strong association. But, the energy spent transmitting this information is a waste of energy. In this paper the densely-concentrated node area is designated as Hotspot_Zone in the multi-hop clustering environment using the AMC and a key node is selected in the area. If the request message of data aggregation is transmitted, the key node among the neighboring nodes sends its environmental information to a manager to avoid duplicate sensing information. Therefore, the life of networks can be prolonged due to this.

• Journal of Korean Society for Atmospheric Environment
• /
• v.21 no.1
• /
• pp.83-96
• /
• 2005

The purposes of this study were to characterize the local levels of VOCs (volatile organic compounds), to develop source profiles of VOCs, and to quantify the source contribution of VOCs using the CMB (chemical mass balance) model. The concentration of VOCs had been measured every 6-day duration in the SRO monitoring site in the Yeosu Petrochemical Industrial Complex from September 2000 to August 2002. The total of 35 target VOCs, which were included in the TO-14 designated from the U.S. EPA, was selected to be monitored in the study area. During a 24-h period, the ambient VOCs were sampled by using canisters placing about 10 ～ 15 m above the ground level. The collected canisters were then analyzed by a GC-MS in the laboratory. Aside from ambient sampling at the SRO site, the VOCs had been intensively and massively measured from 8 direct sources and 4 general sources in the study area. The results obtained in the study were as follows; first, the annual mean concentrations of the target VOCs were widely distributed regardless of monitoring sites in the Yeosu Petrochemical Industrial Complex. In particular, the concentrations of BTX (Benzene, Toluene, Xylene), vinyl chloride were higher than other target compounds. Second, based on these source sample data, source profiles for VOCs were developed to apply a receptor model, the CMB model. Third, the results of source apportionment study for the VOCs in the SRO Site were as follows; The source of petrochemical plant was apportioned by 31.3% in terms of VOCs mass. The site was also affected by 16.7% from wastewater treatment plant, 14.0% from iron mills, 8.4% from refineries, 4.4% from oil storage, 3.8% from automobiles, 2.3% from fertilizer, 2.3% from painting, 2.2% from waste incinerator, 0.6% from graphic art, and 0.4% from gasoline vapor sources.

• Environmental Analysis Health and Toxicology
• /
• v.21 no.4 s.55
• /
• pp.375-380
• /
• 2006

Endocrine disrupting chemicals (EDC) have been emphasized due to their threats in human health. Waste incinerator emission has been emphasized as a source of EDC including polychlorinateddibenzofurans(PCDD/F) and other carcinogenic polycyclic aromatic hydrocarbons (PAHs). Urinary 1-hydroxypyrene (1-OHP) has been used as an exposure biomarker for the PAHs. On the other hand, etheno-DNA adducts, e.g. 1, $N^6-ethenodeoxyadenosine({\varepsilon}dA)$, has been developed as an useful effective or response biomarker for carcinogenesis. Thus, I investigated association between urinary 1-OHP and ${\varepsilon}dA$ levels due to distance from an incinerator which was built more 10 years ago in the middle of a farm in P city. I designated the EDC-high and low exposed group due to distance from the incinerator, i.e. within 2.5km and $5.0{\sim}7.5km$ from the incinerator, respectively. The study subjects were age and sex-matched males and females (mean age, $61.3{\pm}9.6$ yrs; total 40 persons, male, 10; female, 10 for the each group). Urinary 1-OHP and ${\varepsilon}dA$ were analyzed with HPLC-FD and IP-HPLC-FD, respectively. As results, the distance from the incinerator was not associated with urinary 1-OHP nor ${\varepsilon}dA$ levels (p=0.43 and 0.82, respectively). On the other hand, urinary ${\varepsilon}dA$ levels were significantly higher in the hyperlipidemia group (N=10) than normal group (N=30). In conclusion, urinary 1-OHP nor ${\varepsilon}dA$ levels can not be suggested as an incinerator-related exposure nor effective biomarker. However, not only distance from the incinerator bot also systemic approaches including wind and soil contamination are required to assume exposure levels of incinerator-related EDC.

• Resources Recycling
• /
• v.26 no.6
• /
• pp.38-44
• /
• 2017

As by-pass dust (BPD) made from cement manufactured process is designated waste, it is required to bury as high cost. The main component of BPD is potassium chloride (KCl), and used for the fertilizer. For using KCl to the fertilizer, the pH value of KCl is required as neutral or weak acid. However, it is not suitable to apply BPD into the fertilizer directly without any other treatment because BPD's pH value is shown 12.0~12.5; a high base. In this study, the carbon dioxide ($CO_2$) was used for removing calcium oxide (CaO) and reducing pH value during manufacturing process of KCl. We fixed inner condition of the carbon test chamber as $25^{\circ}C$-50RH%, and retained 0~7 hours under the 20 vol% of $CO_2$ atmosphere. After experiment, we analyzed the content of CaO and pH value from each samples. The more time exposed to $CO_2$, the content of CaO and pH value are shown. Furthermore, pH value exposed in 6 hours nearly reached 7.

• Journal of the Korean Wood Science and Technology
• /
• v.45 no.3
• /
• pp.258-267
• /
• 2017

In this study, pitch pine (Pinus rigida, PIR) and Mongolian oak (Quercus mongolica, QUM) pellets were fabricated with bark or/and drying waste liquor (DWL) of larch (Larix kaempferi, LAK) as an additive. Based on the results of fuel characteristics of the pellets, optimal conditions for producing the high-quality pellets were provided. In the analysis of chemical composition, bark contained holocelluose and lignin of 90% and over. DWL had 0.1% solid assumed to sugars which are generated from the oven-drying of LAK logs. QUM showed high ash content (2.2%) by containing of bark in the sawdust. Bark and DWL of LAK had high ash content of 4% and over. Calorific values of all specimens and additives were higher than that of the $1^{st}$-grade standard of wood pellets designated by NIFOS (18.0 MJ/kg). PIR and QUM pellets were fabricated with additive of 2 wt% based on the solid weight of oven-dried sawdust using a piston-type flat-die pelletizer, and thus ash content and calorific value of the pellets did not affect by the use of additive. Durability of the pellets increased with the use of additive. Durabilties of pellets, which were fabricated with bark as an additive and DWL as a controller of moisture content for sawdust, did not differ from those of pellets without additives and were lower than those of pellets either with bark or DWL. However, use of both bark and DWL for the production of wood pellets might be favorable because it can make a profit from the collection process of DWL. Based on the results of fuel characteristics of the pellets, QUM and PIR pellets were produced by a flat-die pelletizer. Moisture content (MC), bulk density and durability of the pellets improved with the use of additive. Particularly, sawdust MC of 10% and the addition of bark or DWL for PIR as well as sawdust MC of 12% and the addition of bark for QUM might be optimal conditions for the production of high-quality pellets. Except for the ash content of QUM pellets, other properties of PIR and QUM pellets exceeded the $1^{st}$-grade wood pellets standards of NIFOS.

• Journal of Marine Bioscience and Biotechnology
• /
• v.7 no.1
• /
• pp.1-10
• /
• 2015

Bio diesel has advantages to reduce GHG(Greenhouse Gas) compare with the fossil fuel by using oil comes from plant/animal sources and even waste such as used cook oil. The diversity of energy feeds brings the positive effects to secure the national energy mix. In this circumstance, micro-algae is one of the prospective source, though some technical barriers. We analyzed the bio diesel which was derived from Dunaliella tertiolecta LB999 through the BD100 quality specifications designated by the law. From that result, it is revealed that the oxidation stability is one of the properties to be improved. In order to find the reason for low oxidation stability, we analyzed the oxidation tendency of each FAME components through some methods(EN 14111, EN14112, EN16091). In this study, we could find the higher double bond FAME portion, the more oxidative property(C18:1${\ll}C18:3$) in bio diesel and main unsaturated FAME group is acted as the key component deciding the bio diesel's oxidation stability. It is proved experimentally that C18:3 FAME are oxidized easily under the modified accelerated oxidation test. We also figure out low molecular weight hydrocarbon and FAME were founded as a result of thermal degradation. Some alcohol and aldehydes were also made by FAME oxidation. In conclusion, it is necessary to find the way to improve the micro-algal bio diesel's oxidation stability.

• Journal of the Korean Applied Science and Technology
• /
• v.33 no.3
• /
• pp.549-559
• /
• 2016

Korean Black body fowl (Gallus gallus domesticus; Ogae) designated as a natural monument (registration number 265) has been known as a superb traditional Korean medicine. In this study, The production of peptide from the Viscera Waste of Yeonsan Ogae was optimized using commercial protease (bromelain) by response surface methodology under high pressure process. The range of processes was pressure (30 to 100 MPa), reaction time (1 to 5 h), and substrate concentration (10 to 30%, w/v). After reaction, the degree of hydrolysis, distribution of amino acids, and molecular weight of peptides were investigated. As a results, the optimization conditions were pressure 90 MPa, reaction time 3 to 4 h, and the amount of viscera meat 20% (w/v), respectively. The molecular weight of protein hydrolysates was distributed 400 to 1,000 Da. Accordingly we presumed that most products were peptides. Of those peptides, nonpolar or hydrophobic, polar but uncharged, positively charged, and negatively charged amino acids were 42.03, 26.0, 13.3, and 18.6%, respectively. Because higher amount of hydrophobic amino acids, we expected that those products would be able to utilize as the functional food ingredients.