• Korean Chemical Engineering Research
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• 제53권2호
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• pp.199-204
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• 2015

지방산 생산을 위한 돈지의 아임계수 가수분해 공정을 최적화하기 위하여 17-run 중심합성법(central composite design)에 기초한 반응표면법(response surface methodology)이 사용되었다. 반응 온도, 오일 대비 물의 몰 비, 반응 시간과 같은 변수 및 이들의 상관관계가 지방산 함량에 미치는 영향을 조사하였으며, 지방산 함량을 예측하기 위하여 2차 다항 회귀방정식이 제안되었다. 최대 지방산 함량을 얻을 수 있는 반응 조건은 $288.5^{\circ}C$, 39.5몰 비, 29.5분이었으며, 이 조건에서의 예측 및 실제 지방산 함량은 각각 97.06% 및 96.99%였다.

• 대한전기학회논문지:전기물성ㆍ응용부문C
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• 제55권10호
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• pp.492-498
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• 2006

A novel surface plasmon resonance(SPR) multisensing method, which does not require imaging apparatus such as CCD, has been proposed and implemented experimentally. The proposed method is based on the multichannel SPR and the separation of signals by use of additional layers whose thickness is controlled. SPR signals are influenced by the thickness of sensing layer as well as the optical condition of sensing surface. As the SPR signals from different ligands are usually positioned closely, the reflected light from sensing surface does not provide us with the clear differences of resonance signal depending on the kinds of ligands. It was found from our experiments that SPR signals from each ligand that is located on the additional layer with different thickness can be separated clearly enough to identify various signals from different ligands. Proposed method with theoretical design and simulation has been verified experimentally by using $SiO_2$ thin film layer as additional layer.

• Fisheries and Aquatic Sciences
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• 제2권1호
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• pp.8-11
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• 1999

The optimum boiling condition was determined for fish extracts by response surface model. Model equations were designed with effect of time (T) and the amount of added water (W) on the level of released free amino acid. Based on the high (>0.9) coefficient of determination and low (<0.01) level of significant, those model was approved to be significant. The added water amount of higher regression coefficient $ (\beta_2)$, showed a greater influence on releasing free amino acids than boiling time. The optimum boiling times are 6 hours for crucian carp, 5 hours for bastard halibut, 7 hours for loach and 5 hours for jacopever. The ratio of added water to sample 1 (v/w) could be applied to all fish samples at $100\pm2^{\circ}C$.

• Archives of Pharmacal Research
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• 제29권9호
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• pp.795-799
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• 2006

Peptides, although high efficacy and specificity in their physiological function, usually have low therapeutical activities due to their poor bioavailability when administrated orally. Nanoparticles have been regarded as a useful vector for targeted drug delivery system because they can protect drug from being degraded quickly and pass the gastrointestinal barriers. Here we described a novel oral N-trimethyl chitosan nanoparticles formulation containing thymopentin (Tp5-TMC-NP). N-trimethyl chitosan (TMC) was synthesized and then used to prepare Tp5-TMC-NP by ionotropic gelation. A three-factor, five-level CCD (Central Composite Design) design was used in the optimization procedure, with HPLC as the analyzing method. The resulting Tp5-TMC-NP had a regular spherical surface and a narrow particle size range with a mean diameter of 110.6 nm. The average entrapment efficiency was 78.8%. The lyophilized Tp5-TMC-NP formulation was stable in $4^{\circ}C\;or\;-20^{\circ}C$ after storage of 3 months without obvious changes in morphology, particle size, pH and entrapment ratio. The results of the flow cytometer determination showed that the ratio of $CD4^+/CD8^+$ of Wistar female rat given Tp5-TMC-NP (ig) was 2.59 time that of the group given Tp5 (ig).

• Integrative Medicine Research
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• 제6권4호
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• pp.388-394
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• 2017

Background: The present study optimized ultrasound-assisted extraction conditions to maximize extraction yields of glycyrrhizic acid from licorice. Methods: The optimal extraction temperature ($X_1$), extraction time ($X_2$), and methanol concentration ($X_3$) were identified using response surface methodology (RSM). A central composite design (CCD) was used for experimental design and analysis of the results to obtain the optimal processing parameters. Results: Statistical analyses revealed that three variables and the quadratic of $X_1$, $X_2$, and $X_3$ had significant effects on the yields and were followed by significant interaction effects between the variables of $X_2$ and $X_3$ (p<0.01). A 3D response surface plot and contour plots derived from the mathematical models were applied to determine the optimal conditions. The optimum ultrasound-assisted extraction conditions were as follows: extraction temperature, $69^{\circ}C$; extraction time, 34?min; and methanol concentration, 57%. Under these conditions, the experimental yield of glycyrrhizic acid was 3.414%, which agreed closely with the predicted value (3.406%). Conclusion: The experimental values agreed with those predicted by RSM models, thus indicating the suitability of the model employed and the success of RSM in optimizing the extraction conditions.

• 한국연소학회:학술대회논문집
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• 한국연소학회 1998년도 제17회 KOSCI SYMPOSIUM 논문집
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• pp.31-36
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• 1998

An experimental study has been performed to characterize fundamental aspects of VOCs removal using non-thermal palsma technique. The removed VOCs in the present study are toluene ($C_6H_5CH_3$), ethene ($C_2H_4$), propene ($C_3H_6$) which are typical air pollutants generated from industry and automobile engines. The non-thermal plasma used in the present experiments has been produced in a wire-cylinder reactor with pulsed corona or a packed-bed reactor filled with ceramic bead. These differently generated non-thermal plasma have been visualized with an intensified CCD. The images of non-thermal plasma have been used for optimal design of a corona reactor used in the present study. The experimental results show that the removal efficiencies of VOCs with non-thermal plasma are dependant on the reactivity of VOCs with OH, O, and $O_3$. The results also show that the removal efficiencies of VOCs decrease significantly when VOCs are treated with NO that is also oxidized in the presence of OH, O, and $O_3$.

• 산업식품공학
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• 제21권3호
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• pp.256-266
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• 2017

This study was designed to optimize ethanol extraction process of unfertilized corn silk (UCS) to maximize phytochemical contents and bioactivities. The response surface methodology (RSM) with central composite design (CCD) was employed to obtain the optimal extraction conditions. The influence of ethanol concentration, extraction temperature and extraction time on total polyphenol contents, total flavonoid contents, maysin contents, 2,2-diphenyl-1-picrylhydrazyl(DPPH) radical scavenging activities and tyrosinase inhibition were analyzed. For all dependable variables, the most significant factor was ethanol concentration followed by extraction temperature and extraction time. The following optimum conditions were determined by simultaneous optimization of several responses with the Derringer's desirability function using the numerical optimization function of the Design-Expert program: ethanol concentration 80.45%, extraction temperature $53.49^{\circ}C$, and extraction time 4.95 h. Under these conditions, the predicted values of total polyphenol contents, total flavonoid contents, maysin contents, DPPH radical scavenging activity and tyrosinase inhibition were $2758.74{\mu}g\;GAE/g$ dried sample, $1520.81{\mu}g\;QUE/g$ dried sample, 810.26 mg/100g dried sample, 56.86% and 43.49%, respectively, and the overall desirability (D) was 0.74.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2002년도 생물공학의 동향 (X)
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• pp.381-384
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• 2002

Response surface methodology (RSM) was successfully applied to optimize for the production of Ganoderma lucidum in batch fermentations using the whey (40,000 mg latose/L) as substrate. This study was performed according to the central composite design (CCD) with respect to pH and temperature, where the designed intervals were 3.3$22.9^{\circ}C$$37.1^{\circ}C$, respectively. A second-order factorial design of the experiments was used to build empirical models providing a quantitative interpretation of the relationships between the two variables. The optimum conditions to maximize the production of G. lucidum were pH 4.2 and $28.3^{\circ}C$. At optimum conditions, the mycelial dry weight (MDW) and residual soluble COD (SCOD) were simultaneously used to evaluate the biokinetic coefficients assocoated with substrate inhibition model by nonlinear least squares method with 95% confidence interval. The. maximum microbial growth rates (${\mu}m$), half saturation coefficient ($K_s$), and the inhibition substrate concentration ($K_{is}$) were determined to be 0.095 l/hr, 128,000 mg SCOD/L and 49,000 mg SCOD/L, respectively. And the microbial yield coefficient (Y), biomass decay rate coefficient ($K_d$), and the maintenance energy coefficient ($m_s$) were determined to be 0.37 mg MDW/mg SCOD, 0.001 1/hr, and 0.0015 1/hr, respectively.

• Journal of the Korean Wood Science and Technology
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• 제45권2호
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• pp.182-194
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• 2017

The optimum alkali pretreatment parameters (reaction time, reaction temperature and potassium hydroxide concentration) for facilitate the conversion into fermentable sugar (glucose) from steam exploded (severity log Ro 2.45) barley husk were determined using Response Surface Methodology (RSM) based on a factorial Central Composite Design (CCD). The prediction of the response was carried out by a second-order polynomial model and regression analysis revealed that more than 88% of the variation can be explained by the models. The optimum conditions for maximum cellulose content were determined to be 201 min reaction time, $124^{\circ}C$ reaction temperature and 0.9% potassium hydroxide concentration. This data shows that the actual value obtained was similar to the predicted value calculated from the model. The pretreated barley husk using acid hydrolysis resulted in a glucose conversion of 94.6%. This research of steam explosion and alkali pretreatment was a promising method to improve cellulose-rich residue for lignocellulosic biomass.

• 한국축산식품학회지
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• 제34권6호
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• pp.836-843
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• 2014

Obesity, a condition in which an abnormally large amount of fat is stored in adipose tissue, causing an increase in body weight, has become a major public health concern worldwide. The purpose of this study was to optimize the process for fermented milk for the production of a functional product with an anti-obesity effect by using Lactobacillus plantarum Q180 isolated from human feces. We used a 3-factor, 3-level central composite design (CCD) combined with the response surface methodology (RSM). Concentration of skim milk powder (%, $X_1$), incubation temperature ($^{\circ}C$, $X_2$), and incubation time (h, $X_3$) were used as the independent factors, whereas pH (pH, $Y_1$), anti-lipase activity (%, $Y_2$) and anti-adipogenetic activity (%, $Y_3$) were used as the dependent factors. The optimal conditions of fermented milk for the highest anti-lipase and anti-adipogenetic activity with pH 4.4 were the 9.5% of skim milk powder, $37^{\circ}C$ of incubation temperature, 28 h of incubation time. In the fermentation condition, the predicted values of pH, anti-lipase activity and anti-adipogenetic activity were 4.47, 55.55, and 20.48%, respectively. However, the actual values of pH, anti-lipase activity and anti-adipogenetic activity were 4.50, 52.86, and 19.25%, respectively. These results demonstrate that 9.5% of skim milk powder and incubation at $37^{\circ}C$ for 28 h were the optimum conditions for producing functional fermented milk with an anti-obesity effect.