• Journal of Plant Biotechnology
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• 제6권1호
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• pp.45-50
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• 2004

Somatic embryos were induced from immature cotyledons and cultured on a MS medium containing 40mg/L 2,4-D. The maximum induction of embryos was obtained from immature cotyledons in a size of 3-4mm, and the highest frequency was obtained in the induction medium at pH 7.0. For embryo development, embryogenic tissues were transferred to a MSM6AC and MSM6 media. Developing embryos were placed at 27$^{\circ}C$with dim light (20$\mu$$molm^{-2}$$s^{-1}$) provided by cool fluorescent tubes (3-D wavelength light is better than standard light). Somatic embryos were clearly developed from globular stage to cotyledonary stages. The color of embryo may be a useful parameter for estimation of embryo quality. When the embryo becomes mature, embryo will be ready for desiccation in order to induce roots and shoots of embryos.

• 한국산림과학회지
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• 제97권5호
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• pp.547-551
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• 2008

The study aimed to develop a cryopreservation method for long-term storage using mature somatic embryo of Japanese larch. In this study, desiccation treatments significantly affected re-induction rates of embryogenic tissue (ET) from dried somatic embryos. In the effect of different dehydration temperature and duration on the re-initiation ET. the highest frequency was shown when somatic embryos were dehydrated at $25^{\circ}C$ for 2 (45.5%) or 1 day (43.3%), respectively. In addition, low temperatures [$4^{\circ}C$, 2 days (44.2%) or 3 days (43.5%)] were marked higher ET initiation. After that, the initiation value was declined with dehydration duration. For comparison of different relative humidity on re-induction frequency of ET, the best re-induction (43.5%) was obtained from somatic embryos pre-dried at $(NH_4)_2SO_4$ (RH 79%). Both $Na_2HPO_4$ (RH 97%) and $Na_2CO_3$ (RH 88%) treatments were showed the similar rate, 34.6, 34.2%, respectively. However the lowest rate (19.6%) was observed in distilled water (RH 100%). In comparison of the various storage temperatures and duration of the dried somatic embryos, the highest frequency (66.9%) of re-initiation was obtained when somatic embryos were cryopreserved for one day. However, the frequency was gradually decreased as the time length of storage increased regardless of types of storage. None of ET re-initiated when stored at $4^{\circ}C$ for 1, 2 and 84 days.

• 한국지반환경공학회 논문집
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• 제1권1호
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• pp.57-63
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• 2000

준설토의 체적변화는 여수토를 통해 물과 함께 빠져나간 토립자의 유실량과 침강되어 새로이 형성된 지반 표면에서의 건조수축과 지반 내에서의 자중압밀에 의한 침하량의 합으로 생각할 수 있다. 그러나 현재까지 체적변화요인과 관련하여 자중압밀과 건조수축에 관한 연구에 비해 토립자의 유실과 관련된 연구는 미미한 실정이다. 따라서, 본 연구에서는 준설토의 체적변화요인 중 여수토를 통해 외부로 유실되는 토량을 보다 명확히 규명하기 위해 준설작업을 실시하기 전의 원지반과 준설토를 투기하여 조성된 매립 지역에서, 각각 채취한 시료에 대한 비중계 분석결과에 Marsal의 수정파쇄율을 적용하여 평균잔류율곡선에 의한 유실률 평가방법을 제안하였고, 이를 검증하기 위하여 기존의 방법을 이용하여 평가한 유실률과 비교 검토해 보았다.

• The Korean Journal of Ecology
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• 제8권2호
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• pp.89-98
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• 1985

Correlations between horizontal distributions of bamboos (Bambusaceae) in the Korean peninsula and environmental factors were studied using taxanomic and geographical literatures, both old and current. The vertical distributions of bamboos on Mt. Chiri were also studied, and environmental factors limiting horizontal and vertical distributions were compared. There are 18 species of bamboos (belonging to 5 genera) distributed in the Korean peninsula. The distributional range of each genus were distinct, although overlapped. Northern limit of bamboos of any species was marked by the line connecting Paikryung Island (124。40'E, 38。00'N), Mt. Changsoo, Mt. Myungji, Mt. Myohyung and Myungchum (129。40'E, 41。10'N). The optimum range of bamboos was concluded to be restricted to several southern province, with annual precipitation over 1,200 mm. The limiting factors on the distribution were inferred to be low temperature and duration of it. Mean daily minimum temperature of January and the number of days with daily mean temperatures below zero during January showed close associations with the distributional range, and an environmental factors favouring the distributrion of bamboos appeared to be vicinity of warm sea current, deep and extended snow acculation and southern exposure. The vertical distribution of bamboos on Mt. Chiri was limited by low temperature, unfavorable topographic and edaphic conditions caused by steep slope. Difference in the vertical limits between SE and NW slopes are caused by the differences in temperature and precipitation between the slopes. Bamboos were more abundant in valleys than on the ridge, apparently because the deeper snow in the valleys protected the plants from low temperature, heavy winter winds and desiccation.

• 한국물환경학회지
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• 제28권1호
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• pp.50-56
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• 2012

In this study, loess composites, loess with lanthanum and with aluminum, were made and evaluated for treatment of phosphorus removal in natural water system. Desiccation method for production of loess composite was superior to centrifugation method in obtaining high concentrated composites of lanthanum and aluminum. Washing of loess lanthanum composite by water did not deteriorat the lanthanum concentration in the composite, but this lowered the aluminum concentration of loess aluminum composite. Total of 15 and 37.5% of aluminum contents were removed after first washing treatment in aluminum loess of 0.05% and 0.1% respectively. However, no more aluminum loss was monitored with increase of washing times. Phosphorus removal efficiencies were not decreased with washed loess aluminum composite. Phosphorus removal was successfully achieved by adsorption of phosphate to loess composite at pH range of 5.0 ~ 8.0. Freundlich and Langmuir adsorption isotherm was observed in the adsorption of phosphate for loess composite. Dosages of 0.05% and 0.1% lanthanum composite for 95% of phosphorus removal could reduce its usage amount to 25% and 50%, respectively, comparing with dosage of loess alone. Dosages of 0.05% and 0.1% aluminum composite could reduce its usage amount to 48% and 63%, respectively.

• Journal of Microbiology and Biotechnology
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• 제20권7호
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• pp.1156-1162
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• 2010

During ethanol fermentation, bacterial strains may encounter various stresses, such as ethanol and acid shock, which adversely affect cell viability and the production of ethanol. Therefore, ethanologenic strains that tolerate abiotic stresses are highly desirable. Bacteria of the genus Deinococcus are extremely resistant to ionizing radiation, ultraviolet light, and desiccation, and therefore constitute an important pool of extreme resistance genes. The irrE gene encodes a general switch responsible for the extreme radioresistance of D. radiodurans. Here, we present evidence that IrrE, acting as a global regulator, confers high stress tolerance to a Zymomonas mobilis strain. Expression of the gene protected Z. mobilis cells against ethanol, acid, osmotic, and thermal shocks. It also markedly improved cell viability, the expression levels and enzyme activities of pyruvate decarboxylase and alcohol dehydrogenase, and the production of ethanol under both ethanol and acid stresses. These data suggest that irrE is a potentially promising gene for improving the abiotic stress tolerance of ethanologenic bacterial strains.

• Journal of Microbiology and Biotechnology
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• 제22권9호
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• pp.1296-1300
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• 2012

The feasibility of using Deinococcus showing strong resistance to both desiccation and ionizing radiation as a dose indicator of gamma radiation exposure was evaluated. Three Deinococcus strains having different levels of radiation resistance, Deinococcus radiodurans (DRD), Deinococcus radiopugnans (DRP), and the DRD pprI mutant (DRM), were selected to develop an appropriate dose indicator for a broad range of exposures. DRD, DRP, and DRM cultures with different numbers of cells [${\sim}10^7$ to $10^3$ colony forming units (CFU)/$100{\mu}l$] were lyophilized and subjected to various doses of gamma radiation to determine a critical dose that inhibited bacterial growth completely. Finally, a combination of DRD at ${\sim}10^7$ and ${\sim}10^6$ CFU, DRP at ${\sim}10^5$ CFU, and DRM at ${\sim}10^4$ CFU successfully indicated exposure to 5, 10, 20, and 30 kGy of gamma radiation, respectively. This study shows the possibility of developing a qualitative indicator of radiation exposure using Deinococcus.

• International Journal of Concrete Structures and Materials
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• 제10권1호
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• pp.113-124
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• 2016

This paper presents the effect of silica fume and nano silica, used individually and in combination with the set accelerator and/or hydrated lime, on the properties of class F high volume ultra fine fly ash (HV-UFFA) cement composites, replacing 80 % of cement (OPC). Compressive strength test along with thermogravimetric analysis, X-ray diffraction and scanning electron microscopy were undertaken to study the effect of various elements on the physico-chemical behaviour of the blended composites. The results show that silica fume when used in combination with the set accelerator and hydrated lime in HV-UFFA cement mortar, improves its 7 and 28 day strength by 273 and 413 %, respectively, compared to the binary blended cement fly ash mortar. On the contrary, when nano silica is used in combination with set accelerator and hydrated lime in HV-UFFA cement mortar, the disjoining pressure in conjunction with the self-desiccation effect induces high early age micro cracking, resulting in hindering the development of compressive strength. However, when nano silica is used without the additives, it improves the 7 and 28 day strengths of HV-UFFA cement mortar by 918 and 567 %, respectively and the compressive strengths are comparable to that of OPC.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2006년도 춘계 학술발표회 논문집
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• pp.1090-1099
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• 2006

Tensile strength of soil is one of the important strength parameters in geotechnical engineering. The importance of the tensile strength of soil has been pointed out and given considerable attention in many highway pavements and earthfill dams. Recently, one of tensile strength problems, cracking failure of clay liners which is related to leakage of polluted water has been reported. Thus, the tensile strength of compacted sand-bentonite mixtures, which are used widely in the landfill construction site as clay liners was measured by Improved Unconfined Penetration (IUP) test. In addition, to find out the effects of contamination levels of water on the tensile strength and desiccation crack pattern, different pH levels of water were used for making specimens prepared with three different mixing contents of bentonite.

• ALGAE
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• 제23권2호
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• pp.91-114
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• 2008

Fifty seven taxa of Cyanobacteria and 15 taxa of Chlorophyta were recorded from the exterior of buildings and rock surfaces of monuments in different regions in India. Four cyanobacteria, e.g. Chroococcidiopsis kashayi, Pseudophormidium indicum, Plectonema puteale and Scytonema geitleri, and the green alga Trentepholia abietina var. tenue occur on the sub-aerial habitats throughout the year. In addition, five other green algae: Chlorococcum infusionum, Scenedesmus arcuatus, Trentepholia aurea, Gloeocystis polydermatica and Printzina effusa, and 18 other cyanobacteria taxa of the genera Chroococcus (5), Asterocapsa (1), Cyanosarcina (2), Gloeocapsa (7), Gloeothece (2) and Scytonema (1) occur on the sub-aerial surfaces enduring extreme temperature and desiccation during summer months of the tropics. During the rainy season, the warm and humid climatic regime coupled with availability of moisture supported an additional ten green algae and 29 cyanobacteria in eight and 17 genera, respectively. The green algal genera Klebsormidium, Stichococcus and Trebouxia, which are dominant in temperate regions, did not occur on the sub-aerial habitats in India, however, species of Gloeocapsa, Chroococcus, Chroococcidiopsis, Phormidium, Leptolyngbya, Nostoc, Scytonema, Chlorella and Trentepholia showed global occurrence in similar habitats.