Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지

Factors Affecting Somatic Embryogenesis from Immature Cotyledon of Soybean

  • Kim, Young Jin (Honam Agricultural Research Institute, National Institute of Crop Science) ;
  • Park, Tae Il (Honam Agricultural Research Institute, National Institute of Crop Science) ;
  • Kim, Hyun Soon (Honam Agricultural Research Institute, National Institute of Crop Science) ;
  • Park, Ho Ki (Honam Agricultural Research Institute, National Institute of Crop Science) ;
  • Chon, Sang Uk (Biotech. Indust. Center, Dongshin Univ.) ;
  • Yun, Song Joong (Faculty of Biological Resources Science, and Institute of Agricultural Science and Technology, Chonbuk National University)
  • Published : 2004.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

Somatic embryos were induced from immature cotyledons and cultured on a MS medium containing 40mg/L 2,4-D. The maximum induction of embryos was obtained from immature cotyledons in a size of 3-4mm, and the highest frequency was obtained in the induction medium at pH 7.0. For embryo development, embryogenic tissues were transferred to a MSM6AC and MSM6 media. Developing embryos were placed at 27$^{\circ}C$with dim light (20$\mu$$molm^{-2}$$s^{-1}$) provided by cool fluorescent tubes (3-D wavelength light is better than standard light). Somatic embryos were clearly developed from globular stage to cotyledonary stages. The color of embryo may be a useful parameter for estimation of embryo quality. When the embryo becomes mature, embryo will be ready for desiccation in order to induce roots and shoots of embryos.

Keywords

References

  1. Bailey, M.A., H.R. Boerma, and W.A. Parrott (1993) Genotype effects on proliferative embryogenesis and plant regeneration of soybean. In Vitro Cell Dev Biol 29:102-108
  2. Barwale, U.B., H.R. Kerns, and J.M. Widholm (1986) Plant regeneration from callus cultures of several soybean genotypes via embryogenesis and organogenesis. Planta 167:473-481
  3. Buchheim, J.A., S.M. Colburn, and J.P. Ranch (1989) Maturation of soybean somatic embryos and the transition to plantlet growth. Plant Physiol 89:7,68-775
  4. Christianson, M.L, D.A. Warnick, and P.S. Carlson (1983) A morphogenetically competent soybean suspension culture. Science 222:632-634
  5. Durham, R.E. and W.A. Parrott (1992) Repetitive somatic embryogenesis from peanut cultures in liquid medium. Plant Cell Rep 11:12-125
  6. Edwards, K.L. and M.H.M. Goldsmith (1980) PH dependent accumulation of indoleacetic acid by corn coleoptile sections. Planta 147:457-466
  7. Finer, J.J. (1988) Apical proliferation of embryogenic tissue of soybean [Glycine max (L.) Merrill]. Plant Cell Rep 7:238-241
  8. Finer, J.J. and A. Nagasawa (1988) Development of an embryogenic suspension culture of soybean (Glycine max Merrill.). Plant Cell Tissue Organ Cult 15:125-136
  9. Hadi, M.Z., M.D. McMullen and J.J. Finer (1996) Transformation of 12 different plasmids into soybean via particle bom-bardment. Plant Cell Rep 15:500-505
  10. Komatsuda, T. and K. Ohyama (1988) Genotypes of high competence for somatic embryogenesis and plant regenera-tion in soybean Glycine max. Theor Appl Genet 75:695-700
  11. Komatsuda, T., K. Kaneko, and S. Oka (1991) Genotype x sucrose interactions for somatic embryogenesis in soybean. Crop Sci 31:333-337
  12. Li, J., and E.A. Grabau (1996) Comparison of somatic em-bryogenesis and embryo conversion in commercial soybean cultivars. Plant Cell Tiss Organ Cult 44:87-89
  13. Liu, W., P.J. Moore, and G.B. Collins (1992) Somatic embryo-genesis in soybean via somatic embryo cycling, In Vitro Cell Dev Biol 28:153-160
  14. Murashige, T. and F. Skoog (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-479
  15. ParroB, W.A., E.G. Williams, D.F. Hildebrand, and G.B. Collins (1989) Effect of genotype on somatic embryogenesis from immature cotyledons of soybean. Plant Cell Tissue Organ Cult 16:15-21
  16. Parrott W.A., G. Dryden, S. Vogt, D.F. Hildebrand, G.B. Collins and E.G. Williams. (1988) Optimization of somatic embryo- genesis and embryo germination in soybean, In vitro Cell & Dev Biol 24:817-820
  17. Parrott, W.A., L.M. Hoffman, D.F. Hildebrand, E.G. Williams, and G.B. Collins (1989) Recovery of primary transformants of soybean. Plant Cell Rep 7:615-617
  18. Samoylov, V.M., D.M. Tucker, and W.A. Parrott (1998) A liquid-medium-based protocol for rapid regeneration from embryogenic soybean cultures. Plant Cell Rep 18:49-54
  19. Santarem, E.R., B. Pelissier, and J.J. Finer (1997) Effect of explant orientation, PH, solidifying agent, and wounding on initiation of soybean somatic embryos, In Vitro Cell Dev Biol-Plant 33:13-19
  20. Santarem, E.R., H.N. Trick, J.S. Essig and J.J. Finer (1998) Sonication-assisted Agrobacterium-mediated transformation of soybean immature cotyledons: optimization of transient expression. Plant Cell Rep 17(10)752-759
  21. Terzi M. and Loschiavo F. (1990) Somatic embryogenesis. In: Bhojwani SS(ed) Development in crop science 19: Plant tissue culture: applications and limitations. Elsevier, Tokyo, pp 54-66
  22. Trick, H.N. and J.J. Finer (1998) Sonication-assisted Agrobacterium-mediated transformation of soybean [Glycine max (L.) Merrill] embryogenic suspension cultures. Plant Cell Rep 17:482-488
  23. Wright, M.S., K.L. Launis, R. Novitzky, J.H. Duesing, and C.T. Harms (1991) A simple method for the recovery of multiple fertile plants from individual somatic embryos of soybean [Glycine max (L.) Merrill]. In Vitro Cell Dev Biol 27:153-157