• Korean Journal of Environmental Agriculture
• /
• v.23 no.3
• /
• pp.123-127
• /
• 2004

In many organisms, trehalose has been Down as an energy source and a protectant against various environmental stresses such as desiccation, freezing, heat and osmotic pressure. Previously, we have isolated and characterized the genes encoding trehalose-6-phosphate synthase (ZrTPS1) and trehalose-6-phosphate phosphatase (ZrTPS2) from one of the most osmotolerant yeasts, Zygosaccharomyces rouxii. We have also generated transgenic plants by co-introduction of ZrTPS2 and ZrTPS2 into potato plant (ZrTPS2-2A-ZrTPS1 plant) in an attempt to metabolically engineer trehalose in the transgenic plant using the foot-and-mouth disease virus(FMDV) 2A system and to generate drought resistant crop plants. In this research, we assayed previously generated the ZrTPS2-2A-ZrTPS1 plant biofunctionally by drought treatment, and measured the amount of trehalose in the ZrTPS2-2A-ZrTPS1 transgenic plants. The ZrTPS2-2A-ZrTPS1 transgenic plant showed strong drought resistance in spite of little or no accumulation of transgenic in he transgenic plant compared with control plant.

• Korean Journal Plant Pathology
• /
• v.14 no.3
• /
• pp.240-246
• /
• 1998

A cDNA encoding desiccation-related protein was isolated from a flower bud cDNA library of Chinese cabbage (C-DH) and its nucleotide sequence was characterized. It contains 679 bp nucleotides with 501 bp open reading frame. The amino acid sequence of the putative protein showed the highest amino acid sequence homology (79 % identity) to dehydrin protein in Gossypium hirsutum. Also, the C-DH shares 48-52% amino acid sequence identity with the other typical dehydrin proteins in plant cells. When the amino acid sequence of their proteins were aligned, several peptide motifs were well conserved, of which function has to be solved. Particularly the C-DH contains 15 additional amino acids at its N-terminus. Genomic Southern blot analysis using the coding region of C-DH showed that the C-DH consists of a single copy gene in Chinese cabbage genome. The C-DH mRNA, whose transcript size is 0.7 kb, was expressed with a tissue-specific manner. It was highly expressed in seed, flower buds and low expression as detected in root, stem or leaf tissues of Chinese cabbage. And the transcript level of C-DH was significantly induced by the treatment of plant hormone, abscisic acid and water-deficit conditions.

• International Journal of Concrete Structures and Materials
• /
• v.8 no.3
• /
• pp.229-238
• /
• 2014

Typical high-performance concrete (HPC) mixtures are characterized by low water-cementitious material ratios, high cement contents, and the incorporation of admixtures. In spite of its superior properties in the hardened state, HPC suffers from many practical difficulties such as its sensitivity to early-age cracking (which is associated with self-desiccation and autogenous shrinkage). In this context, conventional curing procedures are not sufficiently effective to address these limitations. In order to overcome this issue, two strategies,which are based on the use of internal reservoirs of water, have been recently developed.One of these strategies is based on the use of lightweight aggregates (LWA), while the other is based on the use of superabsorbent polymers (SAP). This paper studies and compares the efficiency of the LWA and SAP approaches.Moreover, some of the theoretical aspects that should be taken into account to optimize their application for internal curing of HPC are also discussed. Two fine LWA's and one SAP are studied in terms of autogenous deformation and compressive strength. Increasing the amounts of LWAor SAP can lead to a reduction of the autogenous deformation and compressive strength (especially when adding large amounts). By selecting appropriate materials and controlling their amount, size, and porosity, highly efficient internal water curing can be ensured.

• ALGAE
• /
• v.25 no.1
• /
• pp.37-44
• /
• 2010

A tide-simulating apparatus was developed for culturing marine macroalgae. The objective of this study was to introduce a novel tide-simulating apparatus that can simulate a diurnal or semi-diurnal tidal cycle in the laboratory. In this apparatus, the seaweeds are move up and down and the water level remains the same during the simulated tidal cycle. The apparatus consists of 18 cylindrical culture tanks (3 blocks $\times$ 6 culture tanks) with 12 cm diameter and 24.5 cm long containing up to 2.5 L of seawater. There is a horizontal plate which covered all 18 culture tanks, and it is raised and lowered by a programmable motor that can regulate exposure time. In one application, seaweeds are attached to braided twine hung on Plexiglas air-tubing. The air-tubing is attached to a lid that is set on a horizontal plate. This apparatus is made of colorless Plexiglas to maximize light transmittance. This apparatus is easily disassembled and transportable to any indoor laboratory, wet laboratory, greenhouse, etc. This apparatus also offers considerable flexibility in terms of design. The size of culture tank can be redesigned by either increasing the height of cylinder or/and using a different diameter of cylindrical Plexiglas, therefore, larger/taller thalli can be cultivated. Growth rates of three eulittoral Porphyra species from different tidal elevations have been compared using this device.

• The Microorganisms and Industry
• /
• v.19 no.4
• /
• pp.27-31
• /
• 1993

Certain bacterial species possess the capability of differentiation through several morphogenetic changes which enable them to adapt to certain internal and external stimuli(Losick and Shapiro 1984). Upon induction, cells of A. vinelandii undergo a morphological process which leads to the production of one cyst per cell (Sadoff, 1975). The cysts are considerably resistant to desiccation, which confers a survival advantages upon the organism(Socolofsky and Wyss 1962). Like other prokaryotic differentiations encystment provides a relatively simple model of cellular differentiation. Like in other differentiating bacteria, vegetative growth can be separated from differentiation. Furthermore, the differentiation cycle can be synchronized by specific inducer. There have been a great deal of morphological and physiological studies on this process. However, the mechanisms used to regulate cell differentiation can be clearly defined by careful genetic analysis of the process. Unfortunately, A. vinelandii has proven to be difficult for genetic analysis (Sadoff 1975). For example, it has been shown that a variety of metabolic mutants of Azotobacter speicies are difficult to isolate after mutagenesis with chemical mutagens or UV irradiation. Nevertheless recent advances in molecular genetics in Azotobacter species, especially in the nitrogen fixation research area, appear to be able to overcome this difficulty (Robinson et al. 1986; Kennedy et al. 1986).

• Journal of Microbiology and Biotechnology
• /
• v.20 no.3
• /
• pp.579-586
• /
• 2010

A genomic DNA fragment encoding a putative maltooligosyltrehalose synthase (NfMTS) for trehalose biosynthesis was cloned by the degenerate primer-PCR from cyanobacterium Nostoc flagelliforme. The ORF of NfMTS was 2,799 bp in length and encoded 933 amino acid residues constituting a 106.6 kDa protein. The deduced amino acid sequence of NfMTS contained 4 regions highly conserved for MTSs. By expression of NfMTS in E. coli, it was demonstrated that the recombinant protein catalyzed the conversion of maltohexaose to maltooligosyl trehalose. The $K_m$ of the recombinant enzyme for maltohexaose was 1.87 mM and the optimal temperature and pH of the recombinant enzyme was at $50^{\circ}C$ and 7.0, respectively. The expression of MTS of N. flagelliforme was upregulated, and both trehalose and sucrose contents increased significantly in N. flagelliforme during drought stress. However, trehalose accumulated in small quantities (about 0.36 mg/g DW), whereas sucrose accumulated in high quantities (about 0.90 mg/g DW), indicating both trehalose and sucrose were involved in dehydration stress response in N. flagelliforme and sucrose might act as a chemical chaperone rather than trehalose did during dehydration stress.

• Weed & Turfgrass Science
• /
• v.2 no.3
• /
• pp.292-297
• /
• 2013

Winter turfgrass injury is one of the critical problems of many golf courses in Korea. Turfgrass loss from freezing injury due to low temperature leads to many types of damages including weed invasion, increased herbicide cost, increased soil erosion, and expensive re-establishment. Although Kentucky bluegrass (Poa pratensis L.) which is the most widely used among cool-season grasses in Korea is well known as cold tolerance species, freezing injuries to Kentucky bluegrass during winter are often found. Protecting the turfgrass crown is necessary to recover from low temperature stress in winter because shoots and roots can be recovered from the crown. Turf covers may protect the crowns from direct low temperatures and desiccation. Six different turf covers were installed to cover Kentucky bluegrass during a period of low temperatures. Turf covers had positive effects for spring green-up of Kentucky bluegrass based on the study. Applying any type of turf covers on Kentucky bluegrass resulted in an increase average and minimum temperature compared to the uncovered plot. Among turf covers, clear PVC film without holes produced the longest root length and the highest turfgrass quality.

• Proceedings of the Korean Institute of Building Construction Conference
• /
• 2016.05a
• /
• pp.45-46
• /
• 2016

As the demand for super tall buildings is currently increased in domestic and foreign countries, some kinds of ultra-high strength concretes are being developed actively. Since the cross section of concrete becomes smaller thanks to such kinds of ultra-high strength concretes, the concrete structures can be much bigger, more gigantic and much ultra-high. And as another benefit which is generated thanks to the enhancement of the durability performance, the maintenance expenses are also saved. However, since low W/B ultra-high concrete has a high possibility that many cracks can occur in the initial period due to the self-shrinkage caused by the self-desiccation as one of the blending characteristics, the problem becomes bigger by influencing the safety of a structure. Therefore, in this study, it is intended to analyze the effects of substituting some limestone-based ultra-high strength mortar with electric arc furnace oxidizing slag fine aggregates on the self-shrinkage of mortar.

• Journal of Haehwa Medicine
• /
• v.10 no.1
• /
• pp.79-92
• /
• 2001

According to the literatural study on the Acupuncture & Moxibustion and Traumatherapyof hysterorrhea, the results were as follows. 1. The acupuncture & moxibustion of hysterorrhea is focussed on bloody uterine discharge, leukorrhea and we must carefully diagnose the etiology & the machanism of disease. 2. The causes of hysterorrhea are dampness, impairment of seven emotion, irregular food intake, excessive intercourse and they are impotantly related to liver, spleen, kidney, the ren channel, the chong channel. 3. The diagnosis is grossly divided into the flowing downward of damp-heat, the weaknessof the qi of the spleen, dificiency of yuan of the kidney and according to the each diagnosis, we should select adquate points representing the treatment of cooling(zhongji, yinlingquan, xingjian, etc), desiccation, heiping qi(qihai, zusanli, sanyinjiao, etc), tonificating yang (guanyuan, mingmen, shinshu, etc). 4. The moxibustion is the warming the lower jiao and eliminating the cold, the points are the mingmen point, the zhongji point, the guanyuan point, etc. 5. Besides the acupuncture & moxibustion of hysterorrhea, we can make use of acusetor, ear acupuncture, endermosis, dong shi shen fa. 6. As of traumatherapy of hysterorrhea, fumigation,abluent and soppository are generally used. and the prescriptions as GAMISASANGSAN, BANSUKSAN are used. 7. The medical herbs used on the treatment of hysterorrhea are the tonificating yang, dissipeting, desiccating medicines generally composed of CNIDII FRUCTUS, ALUMEN, ZANTHOXYLI FRUCTUS.

• Journal of Haehwa Medicine
• /
• v.13 no.2
• /
• pp.303-315
• /
• 2004

1. The acupuncture & moxibustion of hysterorrhea is focussed on bloody uterine discharge, leukorrhea and we must carefully diagnose the etiology & the machanism of disease. 2. The causes of hysterorrhea are dampness, impairment of seven emotion, irregular food intake, excessive intercourse and they are impotantly related to liver, spleen, kidney, the ren channel the chong channel 3. Using external medical treatment for Leucorrhea is washing and fumigation on vagina, to wash vagina, to insert vagina, cleansing theraphy and use with Suppository such as YONGYEOMGO(龍鹽膏), KAMISASANGJASAN(加味蛇床子散), SASANGSACHUNGSEJE(蛇床子洗劑). 4. The efficacy of medicines to use external medical treatment is as follow to helpcirculation of blood, to warm spleen and stomach, to warm blood, to warm uterus and remove cold, to remove heat and dry moisture, to down heat-product, to contract bloodvessel, to counteract poison and destory virus, to make enerey and blood.. 5. The diagnosis is grossly divided into the flowing downward of damp-heat, the weaknessof the qi of the spleen, dificiency of yuan of the kidney and according to the each diagnosis we should select adquate points representing the treatment of cooling(zhongji, yinlingquan, xingjian, etc), desiccation, heiping qi(qihai, zusanli, sanyinjiao, etc), tonificating yang (guanyuan, mingmen, shinshu, etc). 6. The moxibustion is the warming the lower jiao and eliminating the cold, the points are the mingmen point, the zhongji point, the guanyuan point, etc 7. Besides the acupuncture & moxibustion of hysterorrhea, we can make use of acusetor, ear acupuncture, endermosis, dong shi shen fa.