• 대한소아치과학회지
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• 제29권3호
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• pp.318-327
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• 2002

뇌성마비인의 구강위생상태에 관한 조사를 위하여 $3{\sim}48$세의 뇌성마비인 총264명(남153명, 여111명)과 비교군으로 비슷한 연령대의 비뇌성마비인 220명(남125명, 여95명)을 대상으로 구강검사를 시행하고, 우식경험유치율(dft rate), 우식경험유치지수(dft index), 우식경험영구치율(DMFT rate), 우식경험영구치지수(DMFT index), 치태지수(plaque index)를 산출하여 비뇌성마비인과 비교하였으며, 지적, 행동, 언어장애의 정도, 유형 및 이환 부위, 거주형태, 보호자의 직업에 따라 분류, 비교하여 다음과 같은 결과를 얻었다. 1. DMFT rate, DMFT index, dft index에서 뇌성마비군과 비뇌성마비군의 유의한 차이가 없었고, dft rate는 유의한 차이로 뇌성마비군이 높게 나타났다. 2. 뇌성마비군의 성별, 유형 및 이환 부위별 비교에서 우식경험도의 유의한 차이를 보이지 않았다. 3. DMFT rate와 DMFT index는 각각의 장애의 정도가 심할수록 유의성 있는 차이로 감소하였으며, 거주형태에서는 수용군이 비수용군에 비해 낮았고, 보호자 직업에서는 자영업이 낮게 나타났다. 4. 치태지수에서 뇌성마비군이 비뇌성마비군보다 유의성 있게 높았고, 거주형태에서는 비수용군이, 보호자 직업에서는 스스로 벌어서 생활하는 경우가 유의성 있는 차이를 보이며 높게 나타났다. 5. 치태지수에서 뇌성마비군의 성별, 유형 및 이환 부위, 각각의 장애의 정도에 대해서는 유의한 차이를 보이고 있지 않았다.

• Journal of Periodontal and Implant Science
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• 제31권2호
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• pp.371-388
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• 2001

A novel glucanhydrolase(DXAMase) from a mutant of Lipomyces starkeyi(KSM 22) has been shown effective in hydrolysis of mutan, reduction of mutan formation by Streptococcus mutans and removal pre-formed sucrose-dependentadherent microbial film and DXAMase has been strongly bound to hydroxyapatitie. These in vitro properties of Lipomyces starkeyi DXAMase are desirable for its application as a dental plaque control agent. This study was performed to determine the adjunctive oral hygiene benefits and safety of dextranase(Lipomyces starkeyi KSM 22 DXAMase)-containing mouthwash when used alongside normal tooth-brushing. This 6-month clinical trial was placebo-controlled double-blind design evaluating 1U/ml dextranase mouthwash and 0.12% chlorhexidine mouthwash. A total 39 systemically healthy subjects, who had moderate levels of plaque and gingivitis were included. At baseline, 1, 3 and 6 months, subjects were scored for plaque accumulation(Turesky modification of Quingley-Hein's plaque index), gingivitis status($L\ddot{o}e$ and Silness gingival index), and tooth stain(Area and severity index system by Lang et al). Additionally, oral mucosal examinations were performed and subjects questioned for adverse symptoms. Two weeks after pre-experiment examinations and a professional prophylaxis, the subjects provided with allocated mousewash and instructed to use 20-ml volumes for 30s twice daily after toothbrushing. All the groups showed significant increase in plaque accumulation since 1 month of experiment. During 6 months' period, the Dextranase mouthwash group showed the least increase in plaque accumulation, compared to the Chlorhexidine mouthwash and placebo groups. As for gingival inflammation, all the groups showed significant increase during 6 months of experiment. The Experimental group(Dextranase mouthwash) also showed the least increase in gingival index score, compared to the Positive control(Chlorhexidine mouthwash)as well as the Negative control(placebo)groups. Whereas the tooth stain was increased significantly in the Positive control group, compared to the baseline score and the Negative controlgroup since 3 months of mouthrinsing. It was significantly increased after 6 months in the Experimental group, still less severe than the Positive control group. As for the oral side effect, the Experimental group showed less tongue accumulation, bad taste, compared to the Positive control group. From these results, mouthrinsing with Lipomyces starkeyi KSM 22 dextranase provided adjunctive benefits to toothbrushing, comparable to 0.12% chlorhexidine mouthwash in inhibition of plaque accumulation and gingival inflammation and local side effects were if anything less frequent and less intense than chlorhexidine, with long-term use of the mouthwash. All data had provided positive evidence for Lipomyces starkeyi KSM 22 dextranase as an antiplaque agent and suggested that further development of dextranase formulations for plaque control are warranted.

• 한국미생물·생명공학회지
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• 제21권3호
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• pp.263-268
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• 1993

Seventeen strains were isolated from soil, cattle rumen, cereal sewage dregs, insect on agar plate containing insoluble glucan as a sole carbon source from immobilized Streptococcus mutans, which produced alpha-1,3 glucanase for lysis of dental plaque. Among these strains isolated from soil, SW-522 and SW-713 that had appeared to produce the high level of alpha-1,3 glucanase, degraded insoluble glucan from S. mutans 97.6% and 49.4%, respectively in 5 hours. The activity of crude alpha-1,3 glucanase from SW-522 was 1.3mg insoluble glucan/min.mg protein. This enzyme was entirely degraded insoluble glucan on glass tube which produced by S. mutans in TH medium with 5% sucrose.

• 치위생과학회지
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• 제12권6호
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• pp.660-665
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• 2012

본 연구는 회전법과 변형 스틸맨법간의 치면세균막 감소효과를 비교하기 위하여 천안 소재 대학교 31명의 대학생을 대상으로 무작위 배정한 순수실험을 설계하여 치면세균막 관리프로그램을 시행한 결과 다음과 같은 결과를 얻었다. 1. 중재 전후에 따른 각 잇솔질의 치면세균막 관리를 비교한 결과 중재 전에 측정한 SPS Score와 ${\Delta}R30$에서 두 잇솔질 방법간의 차이는 나타났으나(p<0.05), 나머지 측정값들과 중재 후의 측정값에서 유의한 관련성을 보이지 않았다(p>0.05). 2. 각 잇솔질 방법에 따른 중재 전 후의 치면세균막 관리를 비교한 결과 PCR, QLF-PCR, ${\Delta}R30$, ${\Delta}R60$에서 회전법과 변형 스틸맨법 집단은 통계적으로 유의한 차이가 있었다(p<0.05). 또한, 회전법보다 변형 스틸맨법 집단에서 치면세균막 지수는 더 큰 감소를 보였고, 잇솔질 교육 중재를 실행한 후 치면세균막 지수가 감소하는 것으로 나타났다. 3. PCR과 QLFD 촬영 측정값의 관계에서 PCR과 육안검사 QLF-PCR의 관계, SPS Score와 그 하위 척도 ${\Delta}R$값들의 상관관계는 보였으나, PCR와 QLF값의 상관관계는 보이지 않았다. 이상의 결과를 종합해보면, 회전법과 변형스틸맨법의 치면세균막 관리 효과는 통계적으로 유의하지 않았으나, 회전법보다 변형 스틸맨법 집단에서 치면세균막 감소차이는 더 큰 것으로 확인되었고, 잇솔질 교육을 실행함으로써 치면세균막 지수가 감소하는 것으로 나타났다.

• Journal of Periodontal and Implant Science
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• 제7권1호
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• pp.22.2-22.2
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• 1977

• 한국치위생학회지
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• 제11권2호
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• pp.263-275
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• 2011

Objectives : This study was performed analysis of Halitosis components following by subjective cognition of halitosis and Oral State in order to provide basic data for halitosis prevention and establish a device to efficiently eliminate halitosis and analyze the factors that affect the halitosis. Methods : 80 adults were participated who visited at the Dental Clinic in Kyoung-gi do, on the period of the March to September in 2010. The obtained results, through the oral examination, halitosis check and interview, Individually, were as followings. Results : According to the ammonia level was a high relationship between sex, CPI, Tongue plaque, DMFT index which were statistically significant (p<0.05). Total oral gas level was a relationship between VAS, tongue plaque, DMFT index(p<0.05). The Correlation coefficient between the Hydrogen sulfide level and Methyl mercaptan was estimated as r=0.534(p<0.01). As for the multiple regression analysis result, there have been selected dimethyl sulfide and Tongue plaque, Gingival index as factors affecting Bridge, Methyl mercaptan level is as factors affecting Hydrogen sulfide level. Conclusions : There was relationship between the periodontal disease and Ammonia gas component, hydrogen sulfide, dimethyl sulfide, methyl mercaptan. Which were statistically significant total oral gas level and oral variables. Such dental caries as the prevention or early treatment of periodontal disease.

• 대한치과의사협회지
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• 제9권12호
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• pp.819-822
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• 1971

For this investigation, author isolated Streptococcus mitis strain SD-9 from the bacterial flora in the human dental plaque, which was incubated in brain-heart infusion media containing 5% sucrose at 37℃ for 24 hours. For the cytochemical demonstration of polysaccharide produced by this strain, a modified thiosemicarbazide osmium method (Critchley et al., 1967) was used. After fixation with this reagent, the harvested cells was suspended in 1% agar for the higher concentration of cells(Kellenberger et al., 1964). And they were dehydrated in the various concentration of ethanol, and embedded in Epon 812(Luft, 1961). Sectioning was done with the Sorvall MT-2 Porter Blum ultramicrotome by means of a glass knife, and the sections were stained with saturated uranyl acetate and lead citrate (Raynolds, 1963). All preparations were examined in a electron microscope, Hitachi HU-ll E-1 type. The morphological features of extracellular polysaccharide produced by Streptococcus mitis strain SD-9 were appeared in 3 structurally different forms, those are, electron dense fibrillar material linearly arranged adjacent to the outer surface of cell wall, highly electron dense globular material adjacent to the outer surface of cell wall, and strutureless fluffy meshwork of possible very fine filament.

• International Journal of Oral Biology
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• 제38권2호
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• pp.55-59
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• 2013

TM7 is an uncultivated organism which is present in extremely diverse environments. Members of the Dialister genus are difficult to culture as a result of which many of these strains remain uncultivated. It has been suggested that TM7 and Dialister bacteria may belong to a group of suspected periodontal pathogens. In our current study, the presence of the sebacteria in Korean dental plaque samples was assessed using PCR detection methods with specific primers for 16S ribosomal RNA genes. The experimental group included 84 volunteers (35 males and 49 females). Plaque samples were collected from 4 non-adjacent proximal sites of the molar areas of the mandible in each subject and pooled. TM7 was detectable in 56% and the Dialister genus in 27.5% of the volunteers. Both TM7 and Dialister were present in 20.3% of volunteers. We found that 36.9% of the volunteers were negative for both bacteria. Further studies to evaluate the prevalence of these putative pathogenic bacteria in the Korean population are warranted.

• 한국치위생학회지
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• 제15권2호
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• pp.189-196
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• 2015

Objectives: The purpose of the study is to investigate the relationship between saliva factors and oral hygiene factors in adults. Methods: The subjects were 112 adults from April 1 to June 15, 2014. The selected salivary factors included stimulated/unstimulated salivary flow rates, salivary buffering capacity and pH, and the selected oral hygiene factors included halitosis, wet weight of tongue plaque and oral humidity in dorsum and inferior surface of tongue. Results: There were significant differences in stimulated/unstimulated salivary flow rates, oral malodor and wet weight of tongue plaque. There were significant differences according to age in stimulated/unstimulated salivary flow rates, salivary buffering capacity and wet weight of tongue plaque. Age had a negative correlation with salivary buffering capacity and had a positive correlation with wet weight of tongue plaque. Unstimulated salivary flow rate had a positive correlation with stimulated salivary flow rate, and stimulated salivary flow rate was positively correlated with oral humidity of inferior surface of tongue, salivary buffering capacity and halitosis. Oral humidity of inferior surface of tongue had a positive correlation with salivary buffering rate, pH and halitosis. Salivary buffering capacity was positively correlated with pH, and pH was negatively correlated with halitosis. Conclusions: The salivary factors were linked to the oral hygiene. As there may be great changes in salivary flow rate and oral hygiene due to various factors, the salivary factors seem to be one of the major factors to ensure oral hygiene and to promote oral health.

• 한국치위생학회지
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• 제13권2호
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• pp.271-277
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• 2013

Objectives : The purpose of this study was to evaluate the effects of incremental dental care(IDC) program on kindergarten children. Methods : The subjects were 46 kindergarten children aged 6 who wished participation in IDC program during 5 weeks at oral health center in G-college from March to May, 2012. The data of subjects were examined into oral conditions such as toothbrushing method and frequency, modified O'leary plaque index and dental caries activity before and after IDC program. The collected data were analyzed by SPSS statistical package version 19.0. Results : 1. The rolling and fones methods of toothbrushing increased from 10.8% to 91.3% after IDC program(p<.001). The change of toothbrushing frequency showed that over 3 times a day increased from 52.2% to 76.0% after IDC program(p<.01). 2. The modified O'leary plaque index of before toothbrushing education increased from 1st visit(score 32.19) to 5th visit(score 57.14) and after toothbrushing education increased from 1st visit(score 64.45) to 5th visit(score 78.27) during IDC program(p<.001). 3. As a results of dental caries activity test before and after IDC program, it was found that the bacterial numbers in S.mutans and Latobacillus reduced from 0.74, 0.70 to 0.28, 0.41 (p<.01) and the saliva buffering capacity increased from 1.50 to 2.02(p<.001). Conclusions : It is considered very necessary that IDC program should be maintained and extended to dental clinic so as to enhance the oral health state of kindergarten children.