• Journal of the korean academy of Pediatric Dentistry
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• v.23 no.4
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• pp.925-930
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• 1996

The purpose of this study was to know whether the information of caries activity and caries experience of the children can be used to predict the behavior of the children during the dental treatment. The subjects of the study were one hundred and eighty-one preschool children, three to six years old. Salivary reductase activity was tested by the Resazurin Disc Test. Caries experience was examined. The children's behavior was observed using the rating scale of Frankl. Salivary reductase activity of the negative behavior group was slightly higher than that of the positive behavior goup, but the difference was not significant. dt index and ds index of the negative behavior group were significantly higher than those of the positive behavior group. There were no significant differences in ft index and fs index between the two behavior groups. The results suggest that the negative behavior of a child can be predicted when the child has many untreated dental caries.

• Journal of Korean society of Dental Hygiene
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• v.13 no.5
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• pp.889-900
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• 2013

Objectives : Molecular biology techniques were employed to assess diversity of bacterial in children's dental caries. Methods : DNA of germs was extracted and the diversity of the 16S rRNA clones was analyzed by amplified rDNA restriction analysis and sequencing. The experimental samples were pit and fissure caries (PC), deep dentinal caries (DC), smooth surface caries (SC), and supragingival plaque (PQ) from 50 children of age less than 12 years old. The control group was healthy teeth supragingival plaque (HT). Thirty clones from each 16S rRNA clone library of 5 samples were randomly selected, thus a total of 150 clones were analyzed. Results : Amplified rDNA restriction analysis uncovered 18, 20, 11, 17, and 22 phylotypes from healthy teeth, pit and fissure caries, deep dentinal caries, smooth surface caries, and supragingival plaque, respectively. Sequencing analysis found the dominance of Actinomycs naeslundii and Fusobacterium nucleatum in the healthy teeth; Leptotrichia sp. in the pit and fissure caries; Actinomyces sp., Streptococcus mutans, and Rahnella aquatilis in the deep dentinal caries; Streptococcus mutans and Actinomyces sp. in the smooth surface caries; Enterobacter hormaechei and Streptococcus sanguinis in the supragingival plaque. Conclusions : Clonal analysis identified 6 phyla, 20 genera, and 51 species.