• Title/Summary/Keyword: density gradient

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Mitochondrial DNA Analysis in Fusants of Ganoderma lucidum and Lentinus edodes (영지버섯과 표고버섯 원형질 융합체의 미토콘드리아 DNA 검색)

  • 최은주;정영자;이영재;김병각;현진원
    • Environmental Mutagens and Carcinogens
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    • v.22 no.3
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    • pp.199-204
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    • 2002
  • It has been known that Ganoderma lucidum and Lentinus edodes have anticancer activity and immune enhancing activity. These two mushrooms were grown in liquid culture and harvested. From these mycelia, DNA was isolated and EtBr-CsCl density gradient ultracentrifugation was performed to purify it further. Then mitochondrial DNA was isolated by bisbenzimide-CsCl density ultracentrifugaton. Mitochondrial DNA of Ganoderma lucidum was digested by restriction enzymes, EcoR I, Hind Ⅲ, and Pst I, then electrophoresed. It showed 12, 22, 4 fragments. Mitochondrial DNA of Lentinus edodes was digested by EcoR I. Electric pattern showed 6 fragments. 4 fragments had appeared by Pst 1 digested mitochondrial DNA. Hind ill couldn't digest mitochondrial DNA of Lentinus edodes. Mitochondrial DNA of fusants was isolated to compare to those of parents. The results showed that fusant P₂S₄has new, recombined mitochondrial DNA. But P₂S₄had the same DNA that Ganoderma lucidum had.

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The physical properties and the dyeability of the easily dyeable polyester yarn under atmospheric pressure (상압가염형 폴리에스테르 섬유의 물성과 염색성)

  • 김태경;윤석환;신상엽;임용진;조규민
    • Textile Coloration and Finishing
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    • v.13 no.6
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    • pp.391-396
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    • 2001
  • The physical properties and the dyeability of the easily dyeable polyester yarn(EDY) were investigated and compared with those of regular polyester (REG-PET). The EDY, copolymerized with small amount of polyethylene glycol(PEG), showed higher intensity of aliphatic CH peak in IR spectrum, lower density and lower compactness than those of the REG-PET from the analysis of IR, density gradient column and XRD respectively. In the physical properties, the EDY has lowers $T_g$, $T_m$, specific stress and initial modulus, and also has higher strain than that of the REG-PET The EDY can be dyed under atmospheric pressure and its dyeing Fate was faster than REG-PET due to low $T_d$, and this seems to be caused by the increased flexibility of Polymer chain in amorphous region of the EDY due to the copolymerization of PEG.

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Analysis of the Ionized Fields around HVDC Transmission Line by the Use of the Charge Simulation Method (전하중첩법을 이용한 직류 송전선 주변 이온장 해석)

  • Min Suk-Won;Song Ki-Hyun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.6 no.6
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    • pp.478-485
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    • 2005
  • This paper analysed the ionized fields around HVDC transmission line by the use of the charge simulation method. As this is very complex and expressed by a non-linear partial differential equations, it is hard to solve problems analytically. So, we developed a computer program which can apply in multi-polar HVDC transmission line with conductor bundles and calculated conductor surface gradient, corona current density and ion charge density to prove validity of a proposed algorithm in this paper.

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Sensitivity Analysis and Optimization of Nonlinear Vehicle Frame Structures (비선형 차체프레임구조물의 민감도해석 및 최적화)

  • Won, Chong-Jin;Lee, Jong-Sun
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.20 no.9
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    • pp.2833-2842
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    • 1996
  • This paper is to practice optimal rigidity design by the strain energy density estimation method for static buckling and sizing design sensitivity analysis for dynamic buckling of a nonlinear vehicle frame structure from those results. Using these sizing design sensitivity resutls, an optimization of a nonlinear vehicle frame structure with dynamic buckling constraint is carrried out with the graient projection method.

Sex selection attempts in rabbits by albumin density gradients (가토(家兎)에 있어서 albumin density gradient에 의한 성선택시도(性選擇試圖))

  • Kim, Myung-cheol
    • Korean Journal of Veterinary Research
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    • v.29 no.1
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    • pp.57-60
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    • 1989
  • This study was carried out to evaluate the possibility of sex preselection by gradients methods using bovine serum albumin in rabbits. Artificial insemination was performed with sperm from the top and bottom layer of rabbit semen separated by bovine serum albumin gradients. Various characteristics of separated sperm, and the conception rate and secondary sex ratio at artificial insemination with separated sperm were compared. The results obtained were as follows. 1. The sperm from the bottom layer showed significanty high value in motility, percent of normal sperm and progressive motility as compared with control sperm and the sperm and the sperm from the top layer. 2. The conception rate of sperm from the bottom layer was higher than that of the top layer. But secondary sex ratio was not altered by this methods.

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Thermodynamics of Mixtures (I). Ideal Gases (혼합물의 열역학 (제1보). 이상기체)

  • C. K. Yun
    • Journal of the Korean Chemical Society
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    • v.17 no.5
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    • pp.324-331
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    • 1973
  • We study a mixture of ideal gases by use of recently developed methods in continuum thermodynamics of irreversible processes. A complete form of the free energy function and the gas law for each component are derived directly from an entropy production inequality by assuming that: (1) Constitutive functions depend on the mass densities, the diffusion velocities, the temperature and its gradient only. (2) Phenomenological coefficients appearing in an extra entropy flux are material constants. (3) The internal energy density per unit mass is independent of the total mass density (Joule).

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Contribution of van der Waals Interactions to the Adsorption Energy of $C_2H_2$, $C_2H_4$, and $C_6H_6$ on Si(100)

  • Kim, Seon-U;Lee, Jun-Ho;Jo, Jun-Hyeong
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.02a
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    • pp.269-269
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    • 2013
  • Usingvan der Waals (vdW) energy-corrected density-functional theory without or with self-consistent screening (SCS) effects, we calculate the adsorption energy of acetylene, ethylene and benzene on Si(100). We find that vdW interactions without SCS effects increase the adsorption energy by 0.23, 0.30, and 0.64 eV for adsorbed $C_2H_2$, $C_2H_4$, and $C_6H_6$ on Si(100), respectively. However, if SCS effects are included, this increase of the adsorption energy is reduced as 0.19, 0.24, and 0.54 eV for the three adsorption systems, respectively. The resulting adsorption energy for each system is between the values computed using the local-density approximation and the generalized-gradient approximation.

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Growth parameters and formation of slip plane in ZnWO4 single crystals by the Czochralski method

  • Lim, Chang-Sung
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.20 no.5
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    • pp.202-206
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    • 2010
  • Single crystals of $ZnWO_4$ were grown successfully in the [100], [010] and [001] directions using the Czochralski method. The growth parameters and the formation of slip plane in $ZnWO_4$ crystals were studied. $ZnWO_4$ crystals had a cleavage plane of (010). The dislocation density on the (010) plane at the center of the crystal was lower than that of the edge region. It was inferred that the high density at the edge of the crystals was caused by the thermal gradient during crystal growth. The etch pit arrangement revealed the (100) slip plane to be most active during crystal growth.

Novel 3D nanofabrication technique and its applications

  • Jeon, Seok-U
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2009.05a
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    • pp.15.1-15.1
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    • 2009
  • Nano transfer printing and micro contact printing is well known printing method based on soft lithography which uses conformal soft elastomer with designed surface relief structures. Here I introduce another class of novel 3D nanofabrication technique by using the same elastomer but in a different manner. The approach, which we refer to as proximity field nanopatterning, uses the surface-reliefed elastomers as phase masks to pattern thick layers of transparent, photosensitive materials. Aspects of the optics, the materials, and the physical chemistry associated with this method are outlined. A range of 3D structures illustrate its capabilities, and several application examples demonstrate possible areas of use in technologies ranging from microfluidics to photonic materials to density gradient structures for chemical release and high-energy density science.

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Metamagnetism in $Fe_3$Al Alloy

  • Rhee, Joo-Yull;Lee, Young-Pak
    • Journal of the Korean Vacuum Society
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    • v.12 no.S1
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    • pp.60-62
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    • 2003
  • In this study we report the results of ab initio first-principles calculations to investigate the possibility of metamagnetic behavior in Fe$_3$Al alloy. We used the WIEN2k package of full-potential linearized-augmented- plane-wave method within the local-spin-density approximation to the density-functional theory. The exchange-correlation functional is the generalized-gradient approximation of Perdew-Burke-Ernzerhof. The theoretical lattice constant, which is about 0.5% smaller than the experimental one, is obtained by minimizing the total energy. If the volume decreases about 9 % from the equilibrium, the total magnetic moment decreases abruptly from 4.6 $\mu_{B}$/f.u. to 4.0 $\mu_{B}$/f.u. Since this change is considerably large (∼14%), it is possible to measure by a simple high-pressure experiment at about 180 kbar.