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The Spermatogenesis of Cichlasoma managuensis, Cichlidae, Teleost (경골어류 시클리드과 Cichlasoma managuensis의 정자형성과정)

  • Lee, Kyu-Jae;Chang, Byung-Soo;Teng, Yung-Chien;Kim, Seok;Song, Mi-Sook;Joo, Kyung-Bok;Kim, Dong-Heui
    • Applied Microscopy
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    • v.39 no.3
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    • pp.219-226
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    • 2009
  • The ultrastructure of spermatogenesis and sperm in Cichlasoma managuensis belonging to Cichlidae was investigated by light and electron microscopes. The testis of C. managuensis contained numerous testicular cysts, and spermatogenesis was synchronized in these testicular cysts. In the case of spermatogonia, the nucleus was comparatively large ellipsoidal, and mitochondria showed a marked development. The size of primary spermatocyte was smaller than that of spermatogonia, and that of secondary spermatocyte was smaller than that of primary spermatocyte. The chromatin of spermatocyte was highly condensed according to their development. The nucleus with electron-dense was round shape. In spermiogenesis, flagella started to be formed and chromatin was more condensed. The mitochondria were rearranged in a middle piece. The sperm was formed by loss of cytoplasm. The head of mature sperm was a spherical shape and had not acrosome. The microtubules of flagella were arranged 9+2 structure. Also, the tail of sperm have lateral fins.

Biocompatibility of experimental mixture of mineral trioxide aggregate and glass ionomer cement (Glass ionomer cement와 혼합한 mineral trioxide aggregate의 생체친화성)

  • Oh, Min-Jae;Jeong, Yu-Na;Bae, In-Ho;Yang, So-Young;Park, Bum-Jun;Koh, Jeong-Tae;Hwang, Yun-Chan;Hwang, In-Nam;Oh, Won-Mann
    • Restorative Dentistry and Endodontics
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    • v.35 no.5
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    • pp.359-367
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    • 2010
  • Objectives: The purpose of the present in vitro study was to evaluate the biocompatibility of mineral trioxide aggregate (MTA) mixed with glass ionomer cement (GIC), and to compare it with that of MTA, GIC, IRM and SuperEBA. Materials and Methods: Experimental groups were divided into 3 groups such as 1 : 1, 2 : 1, and 1 : 2 groups depending on the mixing ratios of MTA powder and GIC powder. Instead of distilled water, GIC liquid was mixed with the powder. This study was carried out using MG-63 cells derived from human osteosarcoma. They were incubated for 1 day on the surfaces of disc samples and examined by scanning electron microscopy. To evaluate the cytotoxicity of test materials quantitatively, XTT assay was used. The cells were exposed to the extracts and incubated. Cell viability was recorded by measuring the optical density of each test well in reference to controls. Results: The SEM revealed that elongated, dense, and almost confluent cells were observed in the cultures of MTA mixed with GIC, MTA and GIC. On the contrary, cells on the surface of IRM or SuperEBA were round in shape. In XTT assay, cell viability of MTA mixed with GIC group was similar to that of MTA or GIC at all time points. IRM and SuperEBA showed significantly lower cell viability than other groups at all time points (p < 0.05). Conclusions: In this research MTA mixed with GIC showed similar cellular responses as MTA and GIC. It suggests that MTA mixed with GIC has good biocompatibility like MTA and GIC.

Effects of Cooking and Drying Methods on the Taste Component and Microstructure of Shrimp (새우의 맛성분과 미세구조에 미치는 가열 및 건조방법의 영향)

  • Kim, Hyun-Ku;Chang, Young-Sang;Shin, Hyo-Sun
    • Applied Biological Chemistry
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    • v.32 no.3
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    • pp.278-285
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    • 1989
  • Effects of cooking and drying methods on the taste component and microstructure of shrimp, Metapenaeus joyneri, were investigated. The nucleotides and their related compounds of fresh shirmp such as ATP, ADP, AMP, IMP, inosine and hypoxanthine were detected. AMP was detected as a trace amount in fresh shrimp, however, it increased up to $23.5{\sim}45.7{\mu}$ moles with cooking and drying due to the decomposition of ATP and ADP to AMP during cooking and drying. The major component of the free amino acids of fresh shrimp was arginine followed by glycine, lysine, proline and alanine. These free amino acids contents were 70% of the total free amino acids. One hundred grams of fresh shrimp contained 1,198mg (dry basis) of the total free amino acids. However, for hot air and freeze dried cooked shrimps it was decreased down to 342mg (dry basis) and 503mg (dry basis), respectively. It might be due to the dissolution of soluble amino acids during cooking. Hot air-and freeze-dried fresh shrimps was higher in hardness and brittleness but lower in cohesiveness and gumminess than hot air-and freeze-dried ones with boiling and microwave heating. Freeze dried shrimp had softer myofibril texture than hot air dried one. At the same time, more dense and multiporous structure in the tissue could be obtained from the hot air and freeze drying, respectively, after microwave heating of shrimps.

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A Study on the Preparation of Powder Coatings Containing Halogen-Free Flame Retardant and Fire Safety (Halogen-Free 난연제를 포함하는 파우더 코팅소재 제조 및 화재안전성 연구)

  • Lee, Soon-Hong;Chung, Hwa-Young;Kim, Dae-In;Noh, Tae-Joon
    • Journal of the Korean Society of Safety
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    • v.26 no.4
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    • pp.47-58
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    • 2011
  • Halogen free intumescent flame retardants(IFRS), such as the mixture of melamine phosphate(MP) and char forming agents(pentaerythritol(PER), di-pentaerythritol(DiPER), tris(2-hydroxyethyl) isocyanurate(THEIC)), were prepared and characterized. Polypropylene(PP)/$IFR_S$ composites were also prepared in the presence of ethylene diamine phosphate(EDAP) as a synergist and used into flame retardant PP powder coatings. Thermoplastic PP powder coatings at 20 wt% flame retardant loading were manufactured by extruded and then mechanical cryogenic crushed to bring them in fine powder form. These intumescent flame retardant powder coatings($IFRPC_S$) were applied on mild steel surface for the purpose of protection and decorative. It is a process in which a $IFRPC_S$ particles coming in contact with the preheated mild steel surface melt and form a thin coating layer. The obtained MP flame retardant was analyzed by utilizing FTIR, solid-state $^{31}P$ NMR, ICP, EA and PSA. The mechanical properties as tensile strength, melt flow index(MFI) and the thermal property as TGA/DTA and the fire safety characteristics as limiting oxygen index(LOI), UL94 test, SEM were used to investigate the effect of $IFRPC_S$. The experimental results show that the presence of $IFR_S$ considerably enhanced the fire retardant performances as evidenced by the increase of LOI values 17.3 vol% and 32.6 vol% for original PP and $IFRPC_S$-3(PP/MP-DiPER/EDAP), respectively, and a reduction in total flaming combustion time(under 15 sec) in UL94 test of $IFRPC_S$. The prepared $IFRPC_S$-3 have good comprehensive properties with fire retardancy 3.2 mm UL94 V-0 level, LOI value 32.6%, tensile strength $247.3kg/cm^2$, surface roughness Ra $0.78{\mu}m$, showing a better application prospect. Through $IFRPC_S$-2(PP/MP-PER/EDAP) and $IFRPC_S$-3 a better flame retardancy than that of the $IFRPC_S$-1(PP/MP/EDAP) was investigated which was responsible for the formed more dense and compact char layer, improved synergy effect of MP and PER/DiPER.

Proposal of Design Criteria on Multi-functional Tunnel for the Urban Traffic Tunnel to Flooding Bypass (도심지 홍수저감과 교통량 분담을 위한 다기능 대심도 터널 설계 기준 방안 제시)

  • Kwon, Soonho;Kim, Junghwan;Chung, Gunhui
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.5
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    • pp.3518-3524
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    • 2015
  • Spatial and time variation of the precipitation in Korea is high, therefore, more than 2/3 of the annual precipitation is concentrated during the rainy season. Climate change also causes the intensive rainfall in the area of dense population, thus the occurrence frequency of the heavy flood in the impervious area has been increased. Therefore, the structural food mitigation measures such as the construction of the higher design frequency stormwater pipes, pumping stations, and/or detention ponds. The flood bypass tunnel or retention storage is also one of the efficient structures to mitigate flood damage in the urban area. However, the economic feasibility has been controversial because the flood bypass tunnel might be used once or twice a year. To solve the problem, the multi-functional tunnel for the urban traffic and flooding bypass has been considered. In this study, the design criteria of the road and water tunnel has been analysed and the composite design criteria is proposed for the multi-functional tunnel which is expected to be constructed.

An Experimental Study for Flexure/Shear Failure Behavior of Composite Beam with GFRP Plank Used As a Permanent Formwork and Cast-in-place High Strength Concrete (영구거푸집으로 사용한 유리섬유 FRP 판과 현장타설 고강도콘크리트로 이루어진 합성보의 휨/전단파괴거동에 관한 실험적 연구)

  • Yoo, Seung-Woon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.6
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    • pp.4245-4252
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    • 2015
  • In this study, an experiment which utilized glass fiber reinforced polymer(GFRP) plank as the permanent formwork of cast-in-place high strength concrete structures was performed. The GFRP plank currently being produced has smooth surface so that it causes problems in behavior with concrete. Therefore, this research analyzed the flexure/shear failure behavior of composite beams, which used GFRP plank as its permanent formwork and has short shear span ratio, by setting the sand coated at GFRP bottom surface, the perforation and interval of the GFRP plank web, and the width of the top flange as the experimental variables. As a result of the experiments for effectiveness of sand attachment in case of not perforated web, approximately 47% higher ultimate load value was obtained when the sand was coated than not coated case and bending/shear failure mode was observed. For effectiveness of perforation and interval of gap, approximately 24% higher maximum load value was seen when interval of the perforation gap was short and the fine aggregate was not coated, and approximately 25% lower value was observed when the perforation gap was not dense on the coated specimen. For effectiveness of top flange breadth, the ultimate load value was approximately 17% higher in case of 40mm than 20mm width.

Effect of Embedment Depth of Footing on Behavior of Compensated Foundation (기초의 근입깊이가 보상기초의 거동에 미치는 영향)

  • Lee, Seung-Hyun;Han, Jin-Tae
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.2
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    • pp.1477-1482
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    • 2015
  • In order to find out the effect of embedment ratio on behavior compensated foundation, numerical analyses were performed. Bearing capacity ratios obtained from numerical analyses were greater than those obtained from theoretical equations and it could be seen that the bearing capacity ratio was proportional to the embedment ratio with only exception of the case of square footing in which bearing capacity ratio was increased rapidly with the embedment ratio. For the case of strip footing on sand, the bearing capacity ratios obtained from the numerical analyses and Meyerhof equation were similar with each other and magnitudes of those were as much as square of the embedment ratio but the bearing capacity ratios were little affected by the embedment ratios for the case of strip footing on clay. It can be said that the bearing capacity ratios obtained from the square footing are greater than those obtained from the strip footing. According to the numerical analysis, values of settlement ratios which correspond to the embedment ratio of one were about 0.4 and settlement ratios were decreased with increase of the embedment ratios. Settlement ratios of the loose sand were smaller than those of the dense sand and the clay.

Property of Sintered Y2O3-stabilized Zirconia from Scrap Powders (폐 상안정 지르코니아 분말로 제조한 소결체의 물성 연구)

  • Song, Oh-Sung;Park, Jong-Sung;Nam, Kyung-Ju
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.10 no.8
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    • pp.1783-1788
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    • 2009
  • We newly propose that we may reuse scrap powders ($Z_rO_2$+8 mol%-$Y_2O_3$) as $Y_2O_3$-stabilized zirconia (YSZ) sintered products through sintering process at 1550$^{circ}C$ for 2hrs. We also prepared the reference specimen from fresh $Z_rO_2$+30 mol%-$Y_2O_3$ powder mixture (celluar type with 1㎛-length). The reference sample showed a dense microstructure with grains of $\sim$10㎛ in diameter, while the sintered sample from scrap powder showed irregular grains of 1$\sim$30 ㎛ in diameter. Through XRD analysis, we confirmed that the reference sample has mixed phases of $Y_2O_3$(cubic), $Z_{r0.8}O_{1.9}$(cubic), and $Z_rO_2$(monoclinic), while the sintered YSZ sample from scrap powder has only tetragonal phase. Moreover, the sintered YSZ from scrap powder showed vickers hardness and apparent density more than 70 and 4.11 g/cc, which implies that it can be suitable for structural material application.

The effect of the strength and wetting characteristics of Bis-GMA/TEGDMA-based adhesives on the bond strength to dentin (2,2-Bis[4-(2-methoxy-3-methacryloyloxy propoxy) phenyl] propane을 함유한 상아질 접착레진의 물성이 접착강도에 미치는 영향)

  • Park, Eun-Sook;Kim, Chang-Keun;Bae, Ji-Hyun;Cho, Byeong-Hoon
    • Restorative Dentistry and Endodontics
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    • v.36 no.2
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    • pp.139-148
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    • 2011
  • Objectives: This study investigated the effect of the strength and wetting characteristics of adhesives on the bond strength to dentin. The experimental adhesives containing various ratios of hydrophobic, low-viscosity Bis-M-GMA, with Bis-GMA and TEGDMA, were made and evaluated on the mechanical properties and bond strength to dentin. Materials and Methods: Five experimental adhesives formulated with various Bis-GMA/Bis-MGMA/TEGDMA ratios were evaluated on their viscosity, degree of conversion (DC), flexural strength (FS), and microtensile bond strength (MTBS). The bonded interfaces were evaluated with SEM and the solubility parameter was calculated to understand the wetting characteristics of the adhesives. Results: Although there were no significant differences in the DC between the experimental adhesives at 48 hr after curing (p > 0.05), the experimental adhesives that did not contain Bis-GMA exhibited a lower FS than did those containing Bis-GMA (p < 0.05). The experimental adhesives that had very little to no TEGDMA showed significantly lower MTBS than did those containing a higher content of TEGDMA (p < 0.05). The formers exhibited gaps at the interface between the adhesive layer and the hybrid layer. The solubility parameter of TEGDMA approximated those of the components of the primed dentin, rather than Bis-GMA and Bis-M-GMA. Conclusions: To achieve a good dentin bond, a strong base monomer, such as Bis-GMA, cannot be completely replaced by Bis-M-GMA for maintaining mechanical strength. For compatible copolymerization between the adhesive and the primed dentin as well as dense cross-linking of the adhesive layer, at least 30% fraction of TEGDMA is also needed.

Structural and Electrical Properties of [(Co1-xCux)0.2(Ni0.3Mn0.7)0.8]3O4 Spinel Thin Films for Infrared Sensor Application (적외선 센서용 [(Co1-xCux)0.2(Ni0.3Mn0.7)0.8]3O4 스피넬 박막의 구조 및 전기적 특성)

  • Lee, Kui Woong;Jeon, Chang Jun;Jeong, Young Hun;Yun, Ji Sun;Cho, Jeong Ho;Paik, Jong Hoo;Yoon, Jong-Won
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.27 no.12
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    • pp.825-830
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    • 2014
  • $[(Co_{1-x}Cu_x)_{0.2}(Ni_{0.3}Mn_{0.7})_{0.8}]_3O_4$ ($0{\leq}x{\leq}1$) thin films prepared by metal organic decomposition process were fabricated on SiN/Si substrate for infrared sensor application. Their structural and electrical properties were investigated with variation of Cu dopant. The $[(Co_{1-x}Cu_x)_{0.2}(Ni_{0.3}Mn_{0.7})_{0.8}]_3O_4$ (CCNMO) film annealed at $500^{\circ}C$ exhibited a dense microstructure and a homogeneous crystal structure with a cubic spinel phase. Their crystallinity was further enhanced with increasing doped Cu amount. The 120 nm-thick CCNMO (x=0.6) thin film had a low resistivity of $53{\Omega}{\cdot}cm$ at room temperature while the Co-free film (x=1) showed a significantly decreased resistivity of $5.9{\Omega}{\cdot}cm$. Furthermore, the negative temperature coefficient of resistance (NTCR) characteristics were lower than $-2%/^{\circ}C$ for all the specimens with $x{\geq}0.6$. These results imply that the CCNMO ($x{\geq}0.6$) thin films are a good candidate material for infrared sensor application.