• Journal of Microbiology and Biotechnology
• /
• 제25권2호
• /
• pp.206-216
• /
• 2015

To clarify the role of surface protective antigen A (SpaA) in the pathogenesis of Erysipelothrix rhusiopathiae C43065 (serotype 2), the spaA deletion mutant of E. rhusiopathiae ${\Delta}spaA$ was constructed by homologous recombination. The virulence of the ${\Delta}spaA$ mutant decreased more than 76-fold compared with that of the wild-type strain C43065 in mice. The mutant strain was sensitive to the bactericidal action of swine serum, whereas the wild-type strain was resistant. The adhesion of wild-type strain to MEF cells was inhibited significantly by treatment with rabbit antiserum against recombinant SpaA (rSpaA) as compared with the treatment with normal rabbit serum, but the mutant strain was not affected. The mutant strain was readily taken up by mouse peritoneal macrophages in the normal rabbit serum, whereas the wild-type strain was resistant. Whereas the rabbit antiserum against rSpaA promoted the phagocytosis of wild-type strain by macrophages, the mutant strain was not affected. In addition, mice vaccinated with the formalin-killed mutant strain were provided 40% protection against challenge by the homologous virulent strain as compared with those with wild-type strain, NaOH-extracted antigen, or rSpaA, which provided more than 80% protection against the same infection. These suggested that SpaA has an important role in the pathogenesis of E. rhusiopathiae infection and could be a target for vaccination against swine erysipelas.

• 한국분말야금학회:학술대회논문집
• /
• 한국분말야금학회 2006년도 Extended Abstracts of 2006 POWDER METALLURGY World Congress Part2
• /
• pp.1223-1224
• /
• 2006

The p-type $(Bi_{0.2}Sb_{0.8})_2Te_3/(Pb_{0.7}Sn_{0.3})$Te functional gradient material (FGM) was fabricated by hot-pressing the mechanically alloyed $(Bi_{0.2}Sb_{0.8})_2Te_3$ and the 0.5 at% $Na_2Te-doped$ $(Pb_{0.7}Sn_{0.3})Te$ powders. Also, the n-type $Bi_2(Te_{0.9}Se_{0.1})_3/PbTe$ FGM was processed by hot-pressing the mechanically alloyed $Bi_2(Te_{0.9}Se_{0.1})_3$ and the 0.3 wt% Bi-doped PbTe powders. With ${\Delta}T$ larger than $300^{\circ}C$, the p-type $(Bi_{0.2}Sb_{0.8})_2Te_3/(Pb_{0.7}Sn_{0.3})Te$ FGM exhibited larger thermoelectric output power than those of the $(Bi_{0.2}Sb_{0.8})_2Te_3$ and the 0.5 at% $Na_2Te-doped$ $(Pb_{0.7}Sn_{0.3})Te$ alloys. For the n-type $Bi_2(Te_{0.9}Se_{0.1})_3/PbTe$ FGM, the thermoelectric output power superior to those of the $Bi_2(Te_{0.9}Se_{0.1})_3$ and the 0.3 wt% Bi-doped PbTe was predicted at ${\Delta}T$ larger than $300^{\circ}C$.

• 기록학연구
• /
• 제32호
• /
• pp.3-44
• /
• 2012

이 논문은 로컬리티 기록화를 위한 디지털 아카이브 구축 방안을 개인과 조직의 참여라는 관점에서 모색하기 위한 것이다. 우선 로컬리티 기록화에 있어서 참여의 유형을 구분하였고 각 유형별 특징과 편익을 검토하였다. 또한 선행연구를 통해 참여형 아카이브의 조건을 살펴보았다. 이 연구에서는 특히 조직의 참여를 통해 구축된 아카이브 사례들을 중심으로 분석하였다. 개인의 참여도 매우 중요한 부분이지만, 지역기록 보존에 대한 인식이 아직 광범위하게 확산되지 않은 우리의 조건에서는 우선 지역 내 공공기관이나 발굴가능한 공동체 아카이브의 역할이 매우 중요하다고 보았기 때문이다. 조직 참여형의 경우, 수집기관의 소장물이 중심이 되고 다수의 수집기관들이 참여하여 구축된 디지털 아카이브, 다수의 공동체 아카이브를 기반으로 구축된 디지털 아카이브로 구분하였다. 영국과 미국에서 구축된 아카이브들 중에서 이러한 두 가지 유형에 해당하는 사례들을 선정하여 조사하였다. 수집기관 기반의 아카이브 사례로는 캘리포니아의 OAC(Online Archives California)와 Calis phere, 캐나다의 MemoryBC, 영국의 People's Collection Wales를, 공동체 기반의 아카이브 사례로는 Connecting Histories, CAW(Community Archives Wales), Cambridgeshire community archive network(CCAN), Norfolk Community Archives Network(NORCAN) 등을 분석하였다. 이러한 사례들은 모두 다수의 수집기관이나 공동체 소장기록을 서비스하는 아카이브 포털의 성격을 가진다. 참여형 아카이브의 조건으로는 ${\Delta}$분산소장 및 통합 활용, ${\Delta}$수집기관 및 이용자의 참여, ${\Delta}$맥락의 제공과 기록의 의미 있는 재현을 설정하였고, 이러한 조건을 중심으로 각 사례 아카이브들을 비교 검토하였다. 특히 유형에 따라 어떤 측면에 강점과 취약점이 있는지도 검토하였다. 이러한 분석을 토대로 국내 환경에서 참여형 로컬리티 아카이브를 구축하는 데에 고려해야 할 시사점을 ${\Delta}$추진주체 및 방식, ${\Delta}$수집기관 및 공동체의 협력 네트워크 구축, ${\Delta}$생산맥락의 보존과 재맥락화, ${\Delta}$평가 선별, ${\Delta}$이용자 참여를 중심으로 제시하였다.

• 한국산학기술학회논문지
• /
• 제16권7호
• /
• pp.4398-4404
• /
• 2015

스테인리스 강판은 동적기계구조물을 제작하기 위한 구조용 재료로 널리 사용된다. 또한 이들을 일체화 시키는 방법으로 가스용접을 많이 사용하게 되는데 가스용접에는 다양한 종류에 의해 구조물을 일체화 시킨다. 따라서 부재와 부재를 연결하는 용접부에 대한 응력분포 및 피로강도평가는 구조물의 건전성 및 수명을 연구하는데 매우 중요한 요소가 된다. 그래서 본 연구에서는 피로시험에 의해서 얻어지는 ${\Delta}P-N_f$ 관계를 유한요소해석법에 의해서 최대주응력으로 ${\Delta}{\sigma}-N_f$ 관계로 나타내어 피로설계기준을 정하였고, 이 결과를 이용해서 확률론적 통계해석기법을 적용해서 가속식을 추정하여 임의의 목표수명을 예측할 수 있는 신뢰성설계기술기법을 제시하고자 하였다.

• 대한치과보철학회지
• /
• 제40권3호
• /
• pp.236-245
• /
• 2002

The acrylic resin was first introduced as denture base materials in 1937 and it is commonly used for denture base fabrication nowadays. Three different curing cycles (Conventional curing cycle, short curing cycle and long curing cycle) and three commercially available heat-activated acrylic resins (Vertex RS, Lucitone 199 and ProBase Hot) were investigated to find the curing cycle and material that showed the minimum shrinkage of the resin during polymerization process. A brass master mold was fabricated and duplicated by additional silicone impression material. Stone molds were made by pouring of type III dental stone (SILKY-ROCK YELLOW, Whip-Mix, Louisville, Kentucky). It was embedded in the flask. Strain gauge and thermocouple were embedded in the specimen. Strain gauge and thermocouple were connected to signal conditioning amplifier and data was recorded by pre-programmed software. The parameters ESmax (Maximum expansion strain), Sb (Strain measured just before deflasking procedure), Sa (Strain measured just after deflasking procedure) and Sf (Strain measured at the end of the experiment) were measured. ${\Delta}$S was calculated from Sb and Sa (${\Delta}$S=Sb-Sa). In the experiment concerned about materials, the parameters 90-ESmax (Maximum expansion strain measured during early 90 minutes of curing procedure), 180-ESmax (Maximum expansion strain measured from 90 minutes to 180 minutes), Sb, Sa, ${\Delta}$S and Sf were measured and the following conclusions were made. 1. The ESmax value of conventional curing cycle showed the largest value and the 180-ESmax value of Lucitone 199 showed the smallest value. 90-ESmax values showed no significant difference (p<0.05). 2. ${\Delta}$S values of conventional curing cycle showed the positive values. ${\Delta}$S values of short curing cycle and long curing cycle showed the negative values. All three materials cured by conventional curing cycle showed the positive values. 3. The Sf values of long curing cycle and ProBase Hot (cured by conventional curing cycle) showed the smallest values.

• 한국세라믹학회지
• /
• 제34권2호
• /
• pp.139-144
• /
• 1997

결정학적으로 T' 구조인 Nd2-xCexCuO4-$\delta$ 회토류 구리산화물 초전도체는 지금까지 매우 복잡한 미세구조를 지닌 것으로써 잘 알려져 있다. 이 구리산화물 초전도체가 초전도 특성을 띠려면, Nd3+ 이온이 Ce4+ 이온에 의해 소량 치환되고 동시에 산소환원이 필요하다. 본 연구에서는 투과전자현미경의 수렴성 전자회절(CBED)패턴을 이용하여 Nd2-xCexCuO4-$\delta$ 의 Ce 미량 첨가와 미량의 산소환원에 따른 결정구조의 전이 발생 여부에 관하여 고찰하였다. 세 가지의 서로 다른 시편 Nd2CuO3,Nd1.85Ce0.15CuO4 와Nd1.85Ce0.15CuO3.965을 고상소결에 의하여 만들어 이들 각각 시편에 대한 CBED패턴을 주사투과전자현미경(STEM)으로 관찰하였다. 그리고 실험에 의하여 얻은 이들 HOLZ선들과 컴퓨터 계산에 의해 그려진 이론적인 HOLZ선들과 비교하는 방법에 의하여 Nd1.85Ce0.15CuO4의 격자상수를 구하였다.

• The Korean Journal of Physiology and Pharmacology
• /
• 제21권6호
• /
• pp.687-694
• /
• 2017

Plumbagin, a hydroxy 1,4-naphthoquinone compound from plant metabolites, exhibits anticancer, antibacterial, and antifungal activities via modulating various signaling molecules. However, its effects on vascular functions are rarely studied except in pulmonary and coronary arteries where NADPH oxidase (NOX) inhibition was suggested as a mechanism. Here we investigate the effects of plumbagin on the contractility of skeletal artery (deep femoral artery, DFA), mesenteric artery (MA) and renal artery (RA) in rats. Although plumbagin alone had no effect on the isometric tone of DFA, $1{\mu}M$ phenylephrine (PhE)-induced partial contraction was largely augmented by plumbagin (${\Delta}T_{Plum}$, 125% of 80 mM KCl-induced contraction at $1{\mu}M$). With relatively higher concentrations (>$5{\mu}M$), plumbagin induced a transient contraction followed by tonic relaxation of DFA. Similar biphasic augmentation of the PhE-induced contraction was observed in MA and RA. VAS2870 and GKT137831, specific NOX4 inhibitors, neither mimicked nor inhibited ${\Delta}T_{Plum}$ in DFA. Also, pretreatment with tiron or catalase did not affect ${\Delta}T_{Plum}$ of DFA. Under the inhibition of PhE-contraction with L-type $Ca^{2+}$ channel blocker (nifedipine, $1{\mu}M$), plumbagin still induced tonic contraction, suggesting $Ca^{2+}$-sensitization mechanism of smooth muscle. Although ${\Delta}T_{Plum}$ was consistently observed under pretreatment with Rho A-kinase inhibitor (Y27632, $1{\mu}M$), a PKC inhibitor (GF 109203X, $10{\mu}M$) largely suppressed ${\Delta}T_{Plum}$. Taken together, it is suggested that plumbagin facilitates the PKC activation in the presence of vasoactive agonists in skeletal arteries. The biphasic contractile effects on the systemic arteries should be considered in the pharmacological studies of plumbagin and 1,4-naphthoquinones.

• 한국정보통신학회:학술대회논문집
• /
• 한국정보통신학회 2015년도 추계학술대회
• /
• pp.405-408
• /
• 2015

오버샘플링 방식을 기본으로 하는 시그마-델타 ADC는 고해상도를 구현할 수 있는 반면 고속 동작 시에는 높은 Signal to Noise and Distortion Ratio (SNDR) 성능을 달성하기 어려운 특성이 있다. 특히 데시메이션 필터의 특성에 따른 고속 ADC의 SNDR의 저하가 크다. 본 논문에서는 고속 동작 시에도 시그마-델타 ADC의 높은 SNDR 제공을 위한 데시메이션 필터의 설계 및 구현을 보인다. 이 데시메이션 필터는 넓은 입력 신호 주파수 대역 내에서 신호의 크기 왜곡을 최소화하기 위해 IIR filter 종류의 Butterworth filter 구조로 구성 하였다. 성능을 검증하기 위해 1-bit, 3차, OSR=64인 시그마-델타 모듈레이터를 포함한 시스마-델타 ADC에 제안된 데시메이션 필터를 적용하여 실험을 하였다. 시뮬레이션 실험을 통해 기존에 널리 쓰이던 CIC(cascaded integrator-comb) 방식의 데시메이션 필터 대비 제안된 Butterworth 구조의 데시메이션 필터가 매우 낮은 통과대역 왜곡을 가지며 따라서 높은 SNDR을 제공한다는 결과를 보인다.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2019년도 추계학술대회
• /
• pp.8-8
• /
• 2019

The stringent response (SR) is characterized as a bacterial defense mechanism in response to various growth-inhibiting stresses. It is activated by accumulation of a small nucleotide regulator, (p)ppGpp, and induces global changes in bacterial transcription and translation. Recent work from our group has shown that (p)ppGpp plays a critical role in virulence and survival in Vibrio cholerae. The genes, relA and relV, are involved in the production of (p)ppGpp, while the spoT gene encodes an enzyme that hydrolyzes it in V. cholerae. A mutant strain defective in (p)ppGpp production (i.e. ${\Delta}relA{\Delta}relV{\Delta}spoT$ mutant) lost the ability to produce cholera toxin (CT) and lost their viability due to uncontrolled production of organic acids, when grown with extra glucose. In contrast, the ${\Delta}relA{\Delta}spoT$ mutant, a (p)ppGpp overproducer strain, produced enhanced level of CT and exhibited better growth in glucose supplemented media via glucose metabolic switch from organic fermentation to acetoin, a neutral fermentation end product, fermentation. These findings indicates that (p)ppGpp, in addition to its well-known role as a SR mediator, positively regulates CT production and maintenance of growth fitness in V. cholerae. This implicates SR as a promising drug target, inhibition of which may possibly downregulate V. cholerae virulence and survival fitness. Therefore, we screened a chemical library and identified a compound that induces medium acidification (termed iMAC) and thereby loss of wild type V. cholerae viability under glucose-rich conditions. Further, we present a potential mechanism by which the compound inhibits (p)ppGpp accumulation. Together, these results indicate that iMAC treatment causes V. cholerae cells to produce significantly less (p)ppGpp, an important regulator of the bacterial virulence and survival response, and further suggesting that it has a therapeutic potential to be developed as a novel antibacterial agent against cholera.

• 한국의류산업학회지
• /
• 제14권4호
• /
• pp.641-647
• /
• 2012

This study investigates the sound properties of fabric frictional sound (SPL, ${\Delta}L$, ${\Delta}f$) according to the film type of PTFE laminated vapor-permeable water-repellent fabrics in order to understand the relationship between SPL and the basic properties of fabrics such as layer, yarn type, and thickness of fiber. This study accesses their mechanical properties and determines how to control them to minimize SPL. Eight PTFE laminated water-repellent fabrics, composed of four different film types (A, B, C, D) and with two different fabrics, were used as test specimens. Frictional sounds generated at 1.21m/s were recorded by using a fabric sound generator and SPLs were analyzed through Fast Fourier Transformation (FFT). The mechanical properties of fabrics were measured by KES-FB. The SPL value was lowest at 74.4dB in film type A and highest as 85.5dB in type D. Based on ANOVA and post-hoc test, specimens were classified into less Loud Group (A, B) and Loud Group (C, D). It was shown that SPL was lower when 2 layer (instead of 3 layer), filament yarn than staple, and thin fiber than thick were used. In Group I, shearing properties (G, 2HG5), geometrical roughness (SMD), compressional properties (LC, RC) and weight (W) showed high correlation with SPL however, elongation (EM) and shear stiffness (G) did with SPL in Group II.