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Electrochemical Studies on Corrosion Inhibition Behaviour of Synthesised 2-acetylpyridine 4-ethyl-3-thiosemicarbazone and Its Tin(IV) Complex for Mild Steel in 1 M HCl Solution

  • Hazani, Nur Nadira;Mohd, Yusairie;Ghazali, Sheikh Ahmad Izaddin Sheikh Mohd;Farina, Yang;Dzulkifli, Nur Nadia
    • Journal of Electrochemical Science and Technology
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    • v.10 no.1
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    • pp.29-36
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    • 2019
  • Corrosion inhibition by synthesised ligand, 2-acetylpyridine 4-ethyl-3-thiosemicarbazone (HAcETSc) and its tin(IV) complex, dichlorobutyltin(IV) 2-acetylpyridine 4-ethyl-3-thiosemicarbazone ($Sn(HAcETSc)BuCl_2$) on mild steel in 1 M hydrochloric acid (HCl) was studied using weight loss measurement, potentiodynamic polarisation, electrochemical impedance spectroscopy (EIS), and scanning electron microscopy (SEM). The inhibition efficiency increases by increasing the inhibitor concentrations. The polarisation study showed that both synthesised compounds were mixed type inhibitors. The electrochemical impedance study showed that the presence of inhibitors caused the charge transfer resistance to increase as the concentration of inhibitors increased. The adsorption of these compounds on mild steel surface was found to obey Langmuir's adsorption isotherm with the free energy of adsorption ${\Delta}G{^o}_{ads}$ of -3.7 kJ/mol and -7.7 kJ/mol for ligand and complex respectively, indicating physisorption interaction between the inhibitors and 1 M HCl solution.

Pneumococcal Δpep27 Immunization Attenuates TLRs and NLRP3 Expression and Relieves Murine Ovalbumin-Induced Allergic Rhinitis

  • Yu, Jae Ik;Kim, Ji-Hoon;Nam, Ki-El;Lee, Wonsik;Rhee, Dong-Kwon
    • Journal of Microbiology and Biotechnology
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    • v.32 no.6
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    • pp.709-717
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    • 2022
  • Allergic rhinitis (AR), one of the most common inflammatory diseases, is caused by immunoglobulin E (IgE)-mediated reactions against inhaled allergens. AR involves mucosal inflammation driven by type 2 helper T (Th2) cells. Previously, it was shown that the Streptococcus pneumoniae pep27 mutant (Δpep27) could prevent and treat allergic asthma by reducing Th2 responses. However, the underlying mechanism of Δpep27 immunization in AR remains undetermined. Here, we investigated the role of Δpep27 immunization in the development and progression of AR and elucidated potential mechanisms. In an ovalbumin (OVA)-induced AR mice model, Δpep27 alleviated allergic symptoms (frequency of sneezing and rubbing) and reduced TLR2 and TLR4 expression, Th2 cytokines, and eosinophil infiltration in the nasal mucosa. Mechanistically, Δpep27 reduced the activation of the NLRP3 inflammasome in the nasal mucosa by down-regulating the Toll-like receptor signaling pathway. In conclusion, Δpep27 seems to alleviate TLR signaling and NLRP3 inflammasome activation to subsequently prevent AR.

Cloning of the β-Lactamase Gene from Bacillus sp. J105 and Analysis of Its Expression in E. colis Cells (Bacillus sp. J105 유래 β-lactamase 유전자의 cloning 및 E. coli 내에서의 발현 분석)

  • Kang, Won-Dae;Lim, Hak-Seo;Seo, Min-Jeong;Kim, Min-Jeong;Lee, Hye-Hyeon;Cho, Kyeong-Soon;Kang, Byoung-Won;Seo, Kwon-Il;Choi, Yung-Hyun;Jeong, Yong-Kee
    • Journal of Life Science
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    • v.18 no.11
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    • pp.1592-1599
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    • 2008
  • The $\beta$-lactamase gene was cloned into E. coli DH5$\alpha$ from Bacillus sp. J105 with strong resistance against $\beta$-lactam antibiotics. The chromosomal DNA was partially digested with Sau3AI and ligated to BamHI digested pLAFR3. $\beta$-Lactamase positive clones were obtained by using in vitro packaging kit. The pKL11-${\Delta}4.6$ with $\beta$-lactamase activity was obtained by subcloning of the recombinant plasmid ($\beta$-lac +). The 6.5 kb fragment in the subcloned plasmid was sequenced. The DNA fragment that contains the $\beta$-lactamase gene encodes 309 amino acids. The 0.17 kb upstream region was similar to those of B. thuringinesis and B. cereus with 97% identity. The deduced amino acids sequence was also similar to those of $\beta$-lactamase from B. thuringinesis and B. cereus with 97% and 94% identity, respectively. The phylogenetic tree also showed the relationships of the $\beta$-lactamase gene of Bacillus sp. J105 to genetically related that of other Bacillus strains. Analysis of expression pattern of the pKL11-${\Delta}4.6$ in E. coli, revealed that the secretion efficiency of $\beta$-lactamase was $4{\sim}5%$ and the molecular weight was as same as that of original $\beta$-lactamase (31 kDa) from Bacillus sp. J105.

Hydrochemistry and Origin of Noble Gases and $CO_2$ Gas Within Carbonated Mineral Waters in the Kyeoungbuk-Kangwon Province, Korea (경북-강원일대 탄산약수의 수질화학과 탄산 및 영족기체 기원)

  • Jeong, Chan-Ho;Yoo, Sang-Woo;Kim, Kyu-Han;Nagao, Keisuke
    • The Journal of Engineering Geology
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    • v.21 no.1
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    • pp.65-77
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    • 2011
  • Hydrochemical and carbon isotopic (${\delta}^{13}C_{DIC}$) analyses of 11 water samples, and noble gas isotopic analyses of 8 water samples collected in the Kyeoungbuk and Kangwon areas of Korea were performed to determine their hydrochemical characteristics and to interpret the source of noble gases and $CO_2$ gas in the water. The carbonated mineral waters are weakly acidic (PH = 5.59-6.04), and electrical conductivity ranges from 302 to $864\;{\mu}S/cm$. The chemical composition of all the water samples is Ca-$HCO_3$ type. The high contents of Fe and Mn exceed the safe limits for drinking water. The ${\delta}^{13}C_{DIC}$ values of the samples range from -5.30‰ to -2.84‰, indicating that the carbon is supplied mainly from a deep-seated source and to a lesser degree from an inorganic carbonate source. The $^3He/^4He$ ratios of the samples range from $1.51{\times}10^{-6}$ to $6.45{\times}10^{-6}$. The samples plot into three groups on a $^3He/^4He$ versus $^4He/^{20}Ne$ diagram: the deep-seated field (e.g., a mantle source), the atmospheric field, and the air-mantle mixing field. A wide range of $^4He/^{20}Ne$ ratios is observed ($0.036{\times}10^{-6}$ to $1.76{\times}10^{-6}$), indicating that while radiogenic $^4He$ is dominant in these water samples, mantle-origin He is also present. The supply of $CO_2$ gas and noble gases from a deep-seated source to carbonated waters is inferred to be controlled by geological structures such as faults and geological boundaries.

THEORETICAL STUDY ON OBSERVED COLOR-MAGNITUDE DIAGRAMS

  • Lee, See-Woo
    • Journal of The Korean Astronomical Society
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    • v.12 no.1
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    • pp.41-70
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    • 1979
  • From $B\ddot{o}hm$-Vitense's atmospheric model calculations, the relations, [$T_e$, (B-V)] and [B.C, (B-V)] with respect to heavy element abundance were obtained. Using these relations and evolutionary model calculations of Rood, and Sweigart and Gross, analytic expressions for some physical parameters relating to the C-M diagrams of globular clusters were derived, and they were applied to 21 globular clusters with observed transition periods of RR Lyrae variables. More than 20 different parameters were examined for each globular cluster. The derived ranges of some basic parameters are as follows; $Y=0.21{\sim}0.33,\;Z=1.5{\times}10^{-4}{\sim}4.5{\times}10^{-3},\;age,\;t=9.5{\sim}19{\times}10^9$ years, mass for red giants, $m_{RG}=0.74m_{\odot}{\sim}0.91m_{\odot}$, mass for RR Lyrae stars, $m_{RR}=0.59m_{\odot}{\sim}0.75m_{\odot}$, the visual magnitude difference between the turnoff point and the horizontal branch (HB), ${\Delta}V_{to}=3.1{\sim}3.4(<{\Delta}V_{to}>=3.32)$, the color of the blue edge of RR Lyrae gap, $(B-V)_{BE}=0.17{\sim}0.21=(<(B-V)_{BE}>=0.18),\;[\frac{m}{L}]_{RR}=-1.7{\sim}-1.9$, mass difference of $m_{RR}$ relative to $m_{RG},(m_{RG}-m_{RR})/m_{RG}=0.0{\sim}0.39$. It was found that the ranges of derived parameters agree reasonably well with the observed ones and those estimated by others. Some important results obtained herein can be summarized as follows; (i) There are considerable variations in the initial helium abundance and in age of globular clusters. (ii) The radial gradient of heavy element abundance does exist for globular clusters as shown by Janes for field stars and open clusters. (iii) The helium abundance seems to have been increased with age by massive star evolution after a considerable amount (Y>0.2) of helium had been attained by the Big-Bang nucleosynthesis, but there is not seen a radial gradient of helium abundance. (iv) A considerable amount of heavy elements ($Z{\sim}10{-3}$) might have been formed in the inner halo ($r_{GC}$<10 kpc) from the earliest galactic co1lapse, and then the heavy element abundance has been slowly enriched towards the galactic center and disk, establishing the radial gradient of heavy element abundance. (v) The final galactic disk formation might have taken much longer by about a half of the galactic age than the halo formation, supporting a slow, inhomogeneous co1lapse model of Larson. (vi) Of the three principal parameters controlling the morphology of C-M diagrams, it was found that the first parameter is heavy clement abundance, the second age and the third helium abundance. (vii) The globular clusters can be divided into three different groups, AI, BI and CII according to Z, Y an d age as well as Dickens' HB types. BI group clusters of HB types 4 and 5 like M 3 and NGC 7006 are the oldest and have the lowest helium abundance of the three groups. And also they appear in the inner halo. On the other hand, the youngest AI clusters have the highest Z and Y, and appear in the innermost halo region and in the disk. (viii) From the result of the clean separations of the clusters into three groups, a three dimensional classification with three parameters, Z, Y and age is prsented. (ix) The anomalous C-M diagrams can be expalined in terms of the three principal parameters. That is, the anomaly of NGC 362 and NGC 7006 is accounted for by the smaller age of the order of $1{\sim}2{\times}10^9$ years rather than by the helium abundance difference, compared with M 3. (x) The difference in two Oosterhoff types I and II can be explained in terms of the mean mass difference of RR Lyrae variables rather than in terms of the helium abundance difference as suggested by Stobie. The mean mass of the variables in Oosterhoff type I clusters is smaller by $0.074m_{\odot}$ which is exactly consistent with Rood's estimate. Since it was found that the mean mass of RR Lyrae stars increases with decreasing Z, the two Oosterhoff types can be explained substantially by the metal abundance difference; the type II has Z<$3.4{\times}10^{-4}$, and the type I has higher Z than the type II.

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Effect of Compaction Method on Induced Earth Pressure Using Dynamic Compaction Roller (진동롤러에 의한 다짐방법이 인접구조물의 다짐토압에 미치는 영향)

  • Roh, Han-Sung
    • International Journal of Highway Engineering
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    • v.3 no.4 s.10
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    • pp.127-136
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    • 2001
  • To increase the structural integrity of concrete box culvert good compaction by the dynamic compaction roller with bi9 capacity is as effective as good backfill materials. It is needed for effective compaction that a compaction roller closes to concrete structure with high frequency. However structural distress of the culvert could be occur due to the excessive earth pressure by great dynamic compaction load. To investigate the characteristics of Induced stress by compaction, a box culvert was constructed with changing cushion materials and compaction methods. Two types of cushion material such as tire rubber chip and EPS(Expanded Polystyrene) were used as cushion panels and they are set on the culverts before backfill construction. Laboratory test result of cushion material says that the value of dynamic elastic modulus of rubber is lesser than that of EPS. On the other hand, material damping of rubber material is greater than that of EPS. In most case, dynamic compaction rollers with 10.5 ton weights were used and vibration frequency was applied 30Hz for the great compaction energy. This paper presents the main results on the characteristics of dynamic earth pressures during compaction. The amounts of induced dynamic pressures$(\Delta\sigma\;h)$ by compaction are affected with construction condition such as compaction frequency, depth of pressure cell, distance between roller and the wall of culvert and roller direction. Based on the measured values dynamic lateral pressure on the culverts, it could be said that orthogonal direction of roller to the length of culvert is more effective to compaction efficiency than parallel direction.

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Gene Expression Profile of T-cell Receptors in the Synovium, Peripheral Blood, and Thymus during the Initial Phase of Collagen-induced Arthritis

  • Kim, Ji-Young;Lim, Mi-Kyoung;Sheen, Dong-Hyuk;Kim, Chan;Lee, So-Young;Park, Hyo;Lee, Min-Ji;Lee, Sang-Kwang;Yang, Yun-Sik;Shim, Seung-Cheol
    • IMMUNE NETWORK
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    • v.11 no.5
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    • pp.258-267
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    • 2011
  • Background: Current management strategies attempt to diagnose rheumatoid arthritis (RA) at an early stage. Transcription profiling is applied in the search for biomarkers for detecting early-stage disease. Even though gene profiling has been reported using several animal models of RA, most studies were performed after the development of active arthritis, and conducted only on the peripheral blood and joint. Therefore, we investigated gene expression during the initial phase of collagen-induced arthritis (CIA) before the arthritic features developed in the thymus in addition to the peripheral blood and synovium. Methods: For gene expression analysis using cDNA microarray technology, samples of thymus, blood, and synovium were collected from CIA, rats immunized only with type II collagen (Cll), rats immunized only with adjuvant, and unimmunized rats on days 4 and 9 after the first immunization. Arrays were scanned with an Illumina bead array. Results: Of the 21,910 genes in the array, 1,243 genes were differentially expressed at least 2-fold change in various organs of CIA compared to controls. Among the 1,243 genes, 8 encode T-cell receptors (TCRs), including CD3${\zeta}$, CD3${\delta}$, CD3${\varepsilon}$, CD8${\alpha}$, and CD8${\beta}$ genes, which were down-regulated in CIA. The synovium was the organ in which the genes were differentially expressed between CIA and control group, and no difference were found in the thymus and blood. Further, we determined that the differential expression was affected by adjuvant more than Cll. The differential expression of genes as revealed by real-time RT-PCR, was in agreement with the microarray data. Conclusion: This study provides evidence that the genes encoding TCRs including CD3${\zeta}$, CD3${\delta}$, CD3${\varepsilon}$, CD8${\alpha}$, and CD8${\beta}$ genes were down-regulated during the initial phase of CIA in the synovium of CIA. In addition, adjuvant played a greater role in the down-regulation of the CD3 complex compared to CII. Therefore, the down-regulation of TCR gene expression occurred dominantly by adjuvant could be involved in the pathogenesis of the early stage at CIA.

Overlapping Region of p53/Wrap53 Transcripts: Mutational Analysis and Sequence Similarity with microRNA-4732-5p

  • Pouladi, Nasser;Kouhsari, Shideh Montasser;Feizi, Mohammadali Hosseinpour;Gavgani, Reyhaneh Ravanbakhsh;Azarfam, Parvin
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.6
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    • pp.3503-3507
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    • 2013
  • Background: Although the majority of investigations concerned with TP53 and its protein have focused on coding regions, recently a set of studies highlighted significant roles of regulatory elements located in p53 mRNA, especially 5'UTR. The wrap53${\alpha}$ transcript is one of those that acts as a natural antisense agent, forming RNA-RNA hybrids with p53 mRNA and protecting it from degradation. Materials and Methods: In this study, we focused on the mutation status of exon $1{\alpha}$ of the WRAP53 gene (according to exon 1 of p53) in 160 breast tumor tissue samples and conducted a bioinformatics search for probable miRNA binding site in the p53/wrap53 overlapping region. Mutations were detected, using single stranded conformation polymorphism (SSCP) and sequencing. We applied the miRBase database for prediction of miRNAs which target overlapping region of p53/wrap53 transcripts. Results: Our results showed all samples to have wild type alleles in exon 1 of TP53 gene. We could detect a novel and unreported intronic mutation (IVS1+56, G>C) outside overlapping regions of p53/wrap53 genes in breast cancer tissues and also predict the presence of a binding site for miR-4732-5p in the 5'UTR of Wrap53 mRNA. Conclusions: From our findings we propose designing further studies focused on overexpression of miRNA-4732-5p and introducing different mutations in the overlapping region of wrap53 and p53 genes in order to study their effects on p53 and its ${\Delta}N$ isoform (${\Delta}$40p53) expression. The results may provide new pieces in the p53 targeting puzzle for cancer therapy.

Reevaluation of hydrogen gas dissolved cleaning solutions in single wafer megasonic cleaning

  • Kim, Hyeok-Min;Gang, Bong-Gyun;Lee, Seung-Ho;Kim, Jeong-In;Lee, Hui-Myeong;Park, Jin-Gu
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2009.11a
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    • pp.34.1-34.1
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    • 2009
  • 1970년대 WernerKern에 의해서 개발된 RCA 습식 세정 공정은 이후 메가소닉 기술 개발과 더불어 현재까지반도체 세정 공정에서 필수 공정으로 알려져 있다. 하지만, 반도체패턴의 고집적화 미세화에 따라 메가소닉을 기반으로 하는 세정기술은 패턴 붕괴 및 나노 입자 제거의 한계를 드러내면서 난관에 봉착하고 있으며, 특히, 기존의 Batch식에서 매엽식으로 세정 방식이 전환은 새로운 개념의 메가소닉 기술 개발을 요구하게 되었다. 메가소닉을 사용한습식 세정공정은 메가소닉에 의한 캐비테이션 효과 (Cavitation Effect)에 따른 충격파 및음압 (Acoustic Streaming)에 의한 입자제거를 주요 메커니즘으로 한다. 메가소닉 주파수와 Boundary Layer 두께는, $\delta=\surd(2v/\omega)$($\delta$=두께, v=유체속도), $\omega=2{\pi}f$ (f=주파수), 으로 표현할 수 있다. 위의 식에 따르면, 메가소닉을 이용한 세정공정에서 주파수가 높아질수록 Boundary Layer의 두께가 감소하며, 이는제거 가능한 입자의 크기가 작아짐을 의미하며, 다시말해, 1 MHz 보다 2 MHz 메가소닉 세정장비에서 미세 입자 세정에 유리함을 예상할 수 있다. 본연구에서는 매엽식 세정장비를 사용하여, 1MHz 및 2MHz 콘-타입 (Cone-Type) 메가소닉 장치를 100nm이하 세정 입자에 대한 입자 제거효율을 평가하였다. 입자 제거 효율을 평가하기 위하여, 표준 형광입자(63nm/104nm 형광입자, Duke Scientifics, USA)를각각 IPA에 분산시킨 후, 실리콘 쿠폰 웨이퍼 ($20mm{\times}20mm$)를 일정시간 동안 Dipping 한 후, 고순도 질소로 건조시켜 오염하였다. 매엽식 세정장비(Aaron, Korea)에 1MHz와 2MHz의 콘-타입메가소닉 발진기 (Durasonic, Korea)를 각각 장착하였다.입자 오염 및 세정 후 입자 개수 측정 및 오염입자의 Mapping은 형광현미경 (LV100D, Nikon, Japan)과 소프트웨어(Image-proPlus, MediaCybernetics, USA)를 사용하여 평가하였으며, Hydrophone을 사용하여 메가소닉에서 발생되는 음압의 균일도를 각 조건에서 측정하였다. 각각의 세정공정은 1MHz와 2MHz 메가소닉 발진기 각각에서 1W, 3W, 5W 파워로 1분간 처리하였으며, 매질을 초순수를 사용하였다. 104nm 형광 입자는 1MHz 와 2 MHz 메가소닉 세정기와 모든 세정 공정조건에서 약 99%의 세정효율인 반면, 63nm 형광입자의 경우는 전체적인세정 결과가 80% 대로 감소하였다. 본 연구를 통하여, 입자크기의 미세화에 따른 입자제거효율이 크게 감소 하는 것을 확인할 수 있으며, 기존 Batch식 메가소닉 대비 단시간 및 낮은 전압에서 동일 혹은높은 세정 효율을 얻었다. 다만, 1MHz와 2MHz 메가소닉에서의 세정력은 큰 차이를 관찰 할 수 없었는데, 주파수변화에 따른 세정효율 측정을 위하여 미세 입자를 사용한 추가 실험이 필요 할 것이다.

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Oxygen Permeation and Mechanical Properties of La0.6Sr0.4Co0.2Fe0.8O3-δ Membrane with Different Microstructures (미세구조에 따른 La0.6Sr0.4Co0.2Fe0.8O3-δ 분리막의 산소투과 및 기계적 특성)

  • Lee, Shi-Woo;Lee, Seung-Young;Lee, Kee-Sung;Woo, Sang-Kuk;Kim, Do-Kyung
    • Journal of the Korean Ceramic Society
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    • v.39 no.10
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    • pp.994-1000
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    • 2002
  • Oxygen permeability and the mechanical properties of mixed ionic-electronic conductive $La_{0.6}Sr_{0.4}Co_{0.2}Fe_{0.8}O_{3-{\delta}}$ perovskite-type membrane, fabricated by solid state reaction, were investigated with regard to microstructure. The microstructure of the membrane was controlled by changing the sintering temperature and holding time. The average grain size and relative density were evaluated as a function of sintering conditions. As the fraction of grain boundary decreased, oxygen permeability showed a tendency to increase. Especially the maximum oxygen flux of 0.37 ml/$cm^2$${\cdot}$min was measured for the specimen sintered at 1300${\circ}C$ for 10 h, which has high density and relatively large grain size. Fracture strength was dependent on the relative density of sintered body, while fracture toughness increased with average grain size.