• 한국정보시스템학회지:정보시스템연구
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• 제12권1호
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• pp.67-85
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• 2003

In the recent a mobile internet makes a change in internet business. The mobile internet service is resulting in M-commerce. Even if M-commerce provides only information service, there will be much possibilities to make applications in various areas such as, delivery service, trading service, banking service etc. in the near future. Delivery service requires many steps in processing, originally. This study is designed to implement a mobile system which makes short in delivery process. It includes that a delivery man receives a signature on a paper and input data at the delivered place by using PDA at hand. The mobile system will be contributed to shorten delivery speed. It is proposed to implement the mobile office system for improving service efficiency in the business of delivery service. This study is designed to implement a mobile system which make short in delivery process. It includes that a delivery man receives a signature on a paper and input data at the delivered place by using PDA at hand. The mobile system will be contributed to shorten delivery speed. It is proposed to implement the mobile office system for improving service efficiency in the business of delivery service.

• Macromolecular Research
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• 제11권1호
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• pp.19-24
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• 2003

Polymeric gene delivery system attracts profound attention as it shows less toxicity, versatility, and reasonable gene expression efficiency. Terplex system, a synthetic biopolymeric gene delivery system consisting of stearyl poly-L-lysine (stearyl-PLL) and low density lipoprotein (LDL) was evaluated for its body distribution of gene expression of exogenously administered pDNA after tail-vein injection in mice. Kidney and spleen are two major organs with highest gene expression, whereas liver and heart showed marginal gene expression among the organs examined. Hemolytic effect of the terplex system was evaluated using human red blood cells, where terplex system did not cause significant hemolysis at the concentrations above the experimental ranges, although unmodified PLL or stearyl-PLL without LDL did. Serum stability of terplex system against enzymatic degradation was also significantly enhanced, presumably due to the steric stabilization from the polymers. Based on these findings and along with its high in vitro transfection efficiency, terplex system could serve as a safe and efficient polymeric gene delivery system with many applications for the in vivo gene therapy.

• International Journal of Oral Biology
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• 제32권1호
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• pp.7-11
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• 2007

It is difficult to introduce DNA in non-invasive manner into oral cancer cells as well as primary cells for gene manipulation and expression in vivo. So far, several methods for a gene delivery have been performed to solve this problem. Magnetofection is one of the recent methods for gene transfer, and nanoparticles are applied under a magnetic field for DNA delivery. We investigated whether the magnetofection increases the efficiency of a gene delivery into several oral cell lines. By using a plasmid coding the green fluorescent protein (GFP), the efficiency of gene transfer by magnetofection was compared with those by using the calcium phosphate and the commercial transfection agent. Indeed, the magnetofection increased the green fluorescent signal in cells, suggested that this method apparently enhance the efficiency of gene delivery without any defects in various oral cancer cell lines. Finally, we have shown that magnetofection can be a useful technique for gene delivery to difficult-to-transfect cells to perform a functional study of genes in vivo.

• KSBB Journal
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• 제31권4호
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• pp.300-311
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• 2016

Recently gene delivery has been designed newly using bioactive biomaterial and applied in the various field by many researchers. In this study, we proposed a new gene delivery system which has the capability of targeting effect in the specific tissue and remarkably enhanced transfection efficiency. We investigated $^1H-NMR$ spectroscopy, particle size analyzer and gel retardation to confirm the correct preparation of gene delivery. Also, we identified the hemo-compatibility of gene delivery by hemolysis assay, non-cytotoxicity by MTT test and transfection efficiency. The uptake mechanism of the gene carrier was confirmed using inhibitor agent such as sodium azide, indomethacin, quercetin, colchicine, and chloropromazine. As a results, it was identified that gene carrier prepared by in this study entered in the cell by the microtubule-dependent, energy-dependent and clathrin-mediated endocytosis pathway.

• Journal of Microbiology and Biotechnology
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• 제22권6호
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• pp.866-871
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• 2012

A novel cholesterol-based cationic lipid containing a tri-2-hydroxyethylamine head group and ether linker (Chol-THEA) was synthesized and examined as a potent gene delivery vehicle. In the preparation of cationic liposome, the addition of DOPE as helper lipid significantly increased the transfection efficiency. To find the optimum transfection efficiency, we screened various weight ratios of DOPE and liposome/DNA (N/P). The best transfection efficiency was found at the Chol-THEA:DOPE weight ratio of 1:1 and N/P weight ratio of 10~15. Most of the plasmid DNA was retarded by this liposome at the optimum N/P weight ratio of 10. The transfection efficiency of Chol-THEA liposome was compared with DOTAP, Lipofectamine, and DMRIE-C using the luciferase assay and GFP expression. Chol-THEA liposome with low toxicity had better or similar potency of gene delivery compared with commercial liposomes in COS-7, Huh-7, and MCF-7 cells. Therefore, Chol-THEA could be a useful non-viral vector for gene delivery.

• 디지털융복합연구
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• 제3권2호
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• pp.73-116
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• 2005

When people contact the government they can use a variety of channels. That is, they go in person to an office, use a telephone service, access information via the Internet, send a letter, or use a third party. Since the Australian Government first recognised the potential of online technology to improve service delivery in its 1997 Investing for Growth statement, it has articulated its policies and strategies for e-government in a number of papers. E-government involves government agencies delivering better programs and services online through the use of new information and communication technologies. The policy papers included Government Online-The Commonwealth's Strategy, launched in April 2000, and a new framework for e-government, Better Services. Better Government, launched in November 2002. Most recently, the Government released Australia's Strategic Framework for the Information Economy in July 2004. These papers outlined the broad directions and priorities for the future of e-government in Australia, and sought to maintain the momentum of agencies' actions under Government Online. One of its key objectives was for agencies to achieve greater efficiency in providing services and a return on their investments in ICT (Information and Communication Technology)-based service delivery. They also stated that investing in e-government should deliver tangible returns, whether they take the form of cost reductions, increased efficiency and productivity, or improved services to business and the broader community Implementation of the Government policy has led to considerable agency investment in ICT-based service delivery. However government policy also requires managers to ensure that program and service delivery is efficient and effective. Efficient and effective use of ICT has the potential to improve service delivery and to make financial savings. This paper outlines how people are using the channels to contact the government in Australia. It also examines the level of satisfaction they have with those services and their preferences and expectations. In addition, this paper aims at identifying the methods used by Australian Government to measure the efficiency and effectiveness of their delivery of services, and at assessing the adequacy of these methods.

• 한국축산시설환경학회지
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• 제6권1호
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• pp.15-22
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• 2000

A monitoring and the forecasting system for the swine delivery was developed using CCD camera multi-function board microphone and data-recorder equipped on a personal computer. For the swine delivery monitoring and forecasting factors four factors were selected such as genitalia swine body shape breast color and sound. Image of physical variation of body shape, shape and color of genitalia area and color of breast of pregnant sow were grabbed using the CCD color camera and multi-function board and variation of voice of pregnant sow was acquired using microphone and data recorder. Acquired information of image and voice were analyzed using a custom developed algorithm and program. The result of the forecasting efficiency of swine delivery was 89%, 71% and 100% using the variation of genitalia are the body shape and the voice of pregnant sow. respectively. The efficiency of image processing was 100% for the delivery detection when the piglet was delivered half of its body from genitalia of pregnant sow, The monitoring and forecasting system informed the estimated time of the delivery of swine to a farm manager immediately if an estimated and established time set by the farm manager was the same and/or the estimated time ws earlier than the established time and the system detected the delivery.

• 한국농공학회지
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• 제39권6호
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• pp.67-79
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• 1997

The perpose of this study was to suggest the experimental characteristic of the Cherepnov Water Lifter following the drain mode. The Cherepnov Water Lifter(CWL), which is powered by the potential energy of water, can be set to operate automatically when the water m a tank is drained. In this study, a CWL is constructed in the valve drain controlling mode(VCM) and the siphon drain controlling mode(SCM), and a pressure transducer is installed. It was found that, in the VCM, intake flow volume is proportional to both delivery flow volume and drain flow volume. In the SCM, intake flow volume is proportional to drain flow volume, and the average delivery rate is proportional to both efficiency and the water utilization ratio. Also, in the VCM, the water utilization ratio is 35~49%, efficiency is 62~9O%, average delivery rate is 12.8~81.2$cm^3$/s, and the average drain rate is 14.O~91.5c$cm^3$/s. On the contrary in the SCM, the water utilization ratio is 1.7~38%, efficiency is 3~58%, average delivery rate is 3.1 ~69.2$cm^3$/s, and the average drain rate is shown as 114.5~ 183$cm^3$/s. As a result of the water utilization ratio, efficiency, average delivery rate, and average drain rate are compared, the VCM is found to be superior and the more economical mode. However, the VCM requires manpower and electricity to operate the electronic machinery involved, while the SCM requires no manpower or electricity at all. An economic evaluation of these differences will be necessary in the future. Also, in the SCM, studies to improve water utilization ratio and efficiency, to find the optimum height of the siphon for decreasing the average drain rate, and to determine the radius of curvature of throat have to be conducted in advance, since a large flow rate is drained during the priming action of the siphon.

• 대한안전경영과학회지
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• 제2권3호
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• pp.61-79
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• 2000

It is very important to delivery timely and safely items in logistics. So, the home delivery service system is introduced by many enterprises. Also, the beginning in 1990's, the rapid increase of automobile increased logistics cost and led to road stagnation. So, the railway transportation use in logistics can be considered. The railway transportation in home delivery service system can overcome the limit of transportation by land. This study evaluate railway transportation in home delivery service system by economical efficiency and timeliness.

• Journal of Advanced Marine Engineering and Technology
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• 제19권3호
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• pp.24-32
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• 1995

The scavenging characteristics have a great influence on the performance of a diesel engine, especially slow-speed two-stroke diesel engines which are usually used as a marine propulsion power plant, and they are greatly affected by the conditions in the cylinder, intake and exhaust manifolds, and the opening and closing timing of scavenging ports or exhaust valves during the gas exchange process. Besides, there are many other factors to affect the scavenging characteristics and these factors interact each other very complicatedly. Therefore the simulation program of the gas exchange process is very useful to improve and predict the scavenging characteristics, due to the high costs associated with redesign and testing. In this paper it was attenpted to investigate the effect of the variation of the pressure ratio of intake to exhaust manifolds, and the variation of the opening and closing timing of a exhaust valve by using a computational program for a three-zone scavenging model which was developed by authors. The computed results showed that the scavenging efficiency and delivery ratio increased considerably, but the trapping efficiency decreased with increasing of the pressure ratio of intake to exhaust manifolds. The scavenging efficiency, trapping efficiency, and th conditions of the cylinder gases were affected by the opening timing of the exhaust valve, but the delivery ratio by the closing timing.