• Journal of Microbiology
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• v.37 no.4
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• pp.248-255
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• 1999

We have analyzed the MRE3/REC114 gene of Saccharomyces cerevisiae, previously detected in isolation of mutants defective in meiotic recombination. We cloned the MRE3/REC114 gene by complementation of the meiotic recombination defect and it has been mapped to chormosome XIII. The DNA sequence analysis revealed that the MRE3 gene is identical to the REC114 gene. The upstream region of the MRE3/REC114 gene contains a T_4C site, a URS (upstream repression sequence) and a TR (T-rich) box-like sequence, which reside upstream of many meiotic genes. Coincidentally, northern blot analysis indicated that the three sizes of MRE3/REC114 transcripts, 3.4, 1.4 and 1.2 kb, are induced in meiosis. A less abundant transcript of 1.4 kb is detected in both mitotic and meiotic cells, suggesting that it is needed in mitosis as well as meiosis. To examine the role of the MRE3/REC114 gene, we constructed mre3 disruption mutants. Strains carrying an insertion or null deletion of the MRE3/REC114 gene showed slow growth in nutrient medium and the doubling time of these cells increased approximately by 2-fond compared to the wild-type strain. Moreover, the deletion mutant (${\delta}$mre3) displayed no meiotically induced recombination and no viable spores. The mre3/rec114 spore lethality can be suppressed by spo13, a mutation that causes cells to bypass reductional division. The double-stranded breaks (DSBs) which are involved in initiation of meiotic recombination were not detected in the analysis of meiotic chromosomal DNA from the mre3/rec114 disruptant. From these results we suggest that the MRE3/REC114 gene product is essential in normal growth and in early meiotic stages involved in meiotic recombination.

• Communications for Statistical Applications and Methods
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• v.14 no.1
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• pp.229-239
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• 2007

We present a diagnostic method for the quasi-likelihood estimators in generalized linear models. Since these estimators can be usually obtained by iteratively reweighted least squares which are well known to be very sensitive to unusual data, a diagnostic step is indispensable to analysis of data. We extend the local influence approach based on the maximum likelihood function to that on the quasi-likelihood function. Under several perturbation schemes local influence diagnostics are derived. An illustrative example is given and we compare the results provided by local influence and deletion.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2005.04a
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• pp.85-85
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• 2005

• Communications for Statistical Applications and Methods
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• v.2 no.1
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• pp.74-88
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• 1995

This article introduces a unified method of choosing the most explanatory and significant multiway partitions for classification tree design and analysis. The method is derived on the impurity reduction (IR) measure of divergence, which is proposed to extend the proportional-reduction-in-error (PRE) measure in the decision-theory context. For the method derivation, the IR measure is analyzed to characterize its statistical properties which are used to consistently handle the subjects of feature formation, feature selection, and feature deletion required in the associated classification tree construction. A numerical example is considered to illustrate the proposed approach.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.16 no.6
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• pp.517-532
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• 1991

This paper shows performance analysis for FH/MF나 systems over the cellular mobile communication channel. The Channel capacity over FH/MF나 system is represented as a function of deletion probalblity (Po) and insertion probalbility(Pt). The spectral efficiency of the system serves as a per formance of cellular system is inferior to that of non cellular system. In the case of frequency reuse, we get the best performance when the reuse pattern(N) is 3.

• Proceedings of the Korea Information Processing Society Conference
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• 2007.05a
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• pp.1100-1101
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• 2007

This paper proposes an efficient mechanism of node membership verification within the groups of sensors in a wireless sensor network (WSN). We utilize one-way accumulator to check the memberships of the legitimate nodes in a secure way. Our scheme also supports the addition and deletion of nodes in the groups in the network. Our analysis shows that, our scheme could be well-suited for the resource constrained sensors in a sensor network and it provides a lightweight mechanism for secure node membership verification in WSN.

• Journal of Korean Institute of Industrial Engineers
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• v.20 no.4
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• pp.73-84
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• 1994

Output analysis is one of the most important fields of simulation to achieve the accurate simulation results. This study shows how to analyze simulation output data in the steady state using Procrustes analysis technique which has not been used in the field of simulation yet. In this paper Procrustes analysis method is used to perform the analysis of simulation output efficiently and effectively by applying the improved version of the method. The experiments are conducted using M/M/1 queueing simulation model. The results obtained by Procrustes analysis method show better estimates for average waiting times and average queue lengths which are closer to true values and narrower confidence intervals than when replication-deletion method is used. Also it requires the smaller number of simulation runs.

• Korean Journal of Microbiology
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• v.38 no.2
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• pp.86-95
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• 2002

As a result of genome projects, the research to elucidate the function of a protein of interest has recently been well-recognized. In order to facilitate functional genomics, a useful mammalian gene expression vector is required. Using an infectious CDNA clone of BVDV pNADLclns-, we have developed a mammalian gene expression vector. In this study, a replication-competent full-length infectious CDNA clone containing puremycin acetyltransferase (pac) gene (pNADLclns-/pac) was successfully generated. The viral RNA replication and viral protein NS3 synthesis were examined by detecting metabollically $^{32}P$-labelled genomic viral RNA and immunoblotting with a mouse anti-NS3 antibody. To generate viral replicon as an expression vector, we examine if the viral structural genes (C, E0, El, E2) are required for viral replication by deletion analysis. As a result, all of the structural proteins are dispensable for viral replication per se, but essential for infectious viral particle formation. Based on our deletion analysis, we have generated a replication-competent BVDV viral replicon (pNADLclns-/pac/${\Delta}S$), whose structural genes are all deleted. In addition to NADLclns- /pac/${\Delta}S$, NADLclns-/ luc/${\Delta}S$ viral replicon containing luciferase gene as a reporter was constructed and fecund to be replication-compotent in HeLa and BHK cells as well as MDBK cells. Therefore, BVDV viral replicon developed in our study will be a useful tool to express a protein of interest in various mammalian cells.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.12
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• pp.4915-4920
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• 2015

Background: : Familial adenomatous polyposis (FAP) is an autosomal dominant inherited disease mainly caused by mutations of the adenomatous polyposis coli (APC) gene with almost complete penetrance. These colorectal polyps are precancerous lesions that will inevitable develop into colorectal cancer at the median age of 40-year old if total proctocolectomy is not performed. So identification of APC germline mutations has great implications for genetic counseling and management of FAP patients. In this study, we screened APC germline mutations in Chinese FAP patients, in order to find novel mutations and the APC gene germline mutation characteristics of Chinese FAP patients. Materials and Methods: The FAP patients were diagnosed by clinical manifestations, family histories, endoscope and biopsy. Then patients peripheral blood samples were collected, afterwards, genomic DNA was extracted. The mutation analysis of the APC gene was conducted by direct polymerase chain reaction (PCR) sequencing for micromutations and multiplex ligation-dependent probe amplification (MLPA) for large duplications and/or deletions. Results: We found 6 micromutations out of 14 FAP pedigrees, while there were no large duplications and/or deletions found. These germline mutations are c.5432C>T(p. Ser1811Leu), two c.3926_3930delAAAAG (p.Glu1309AspfsX4), c.3921_3924delAAAA (p.Ile1307MetfsX13), c3184_3187delCAAA(p.Gln1061AspfsX59) and c4127_4126delAT (p.Tyr1376LysfsX9), respectively, and all deletion mutations resulted in a premature stop codon. At the same time, we found c.3921_3924delAAAA and two c.3926_3930delAAAAG are located in AAAAG short tandem repeats, c3184_3187delCAAA is located in the CAAA interrupted direct repeats, and c4127_4128 del AT is located in the 5'-CCTGAACA-3', 3'-ACAAGTCC-5 palindromes (inverted repeats) of the APC gene. Furthermore, deletion mutations are mostly located at condon 1309. Conclusions: Though there were no novel mutations found as the pathogenic gene of FAP in this study, we found nucleotide sequence containing short tandem repeats and palindromes (inverted repeats), especially the 5 bp base deletion at codon 1309, are mutations in high incidence area in APC gene,.

• Journal of Korean Society of Steel Construction
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• v.12 no.5 s.48
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• pp.475-485
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• 2000

In this study, an automated optimum design program for steel box girder bridges has been developed for the optimum design of composite steel box girder bridges. The design constraints required for the optimum design of steel box girder bridges are based on the Korean standard bridge specification. Considering characteristics of steel box girder bridges, several approximation techniques, such as artificial constraint deletion, variable linking and stress reanalysis technique etc. are also introduced to enhance the efficiency of optimization. The developed program is mainly composed of major sub-system modules including structural analysis module using commercial structural analysis program such as RM-SPACEFRAME, optimum design module, pre-process module for friendly user input, and post-processor module for office automation. In addition, in order to demonstrate the efficiency and applicability of the developed optimum design program for steel box girder bridges, a few numerical examples are applied. Based on the results of the application, it may be stated that the automatic optimum design program developed in this study can be a prototype model for the developement of optimum design program for other type of bridge.