• Biomolecules & Therapeutics
• /
• v.21 no.5
• /
• pp.391-397
• /
• 2013

In the present study, we aimed to analyze the antinociceptive, immunomodulatory and antipyretic activities of nymphayol were investigated in wistar rats and mice. Antinociceptive effect was evaluated by acetic acid induced writhing, formalin induced paw licking and hot-plate tests. Immunomodulatory activity was assessed by neutrophil adhesion test, humoral response to sheep red blood cells, delayed-type hypersensitivity, phagocytic activity and cyclophosphamide induced myelosuppression. Antipyretic activity was evaluated by yeast induced hyperthermia in rats. Nymphayol produced significant (p<0.05) antinociceptive activity in acetic acid induced writhing response and late phase of the formalin induced paw licking response. Pre-treatment with nymphayol (50 mg/kg, oral) evoked a significant increase in neutrophil adhesion to nylon fibres. The augmentation of humoral immune response to sheep red blood cells by nymphayol (50 mg/kg) was evidenced by increase in antibody titres in rats. Oral administration of nymphayol (50 mg/kg) to rats potentiated the delayed-type hypersensitivity reaction induced by sheep red blood cells. Treatment with nymphayol showed a significant (p<0.05) reduction in pyrexia in rats. The results suggest that nymphayol possesses potent anti-nociceptive, immunomodulatory and antipyretic activities.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
• /
• v.6 no.1
• /
• pp.15-29
• /
• 1993

Yenhwagamchotang has been widely used in treatment of inflammatory disease which is based on Oriental Medical literatures. These studies were attempted experimental effects of Yenhwagamchotang(sample A), Yenhwagamchotang plus Forsythia koreana NAKAI.(sample B), and Yenhwa-gamchotang plus Taraxacum mongolicum HAND- MAZZ(sample C),on the Anti-allergic reaction, the antipyretic action,the anti-inflammatory and the analgesic action,in rats. THe results of the studies were as follow: 1. Vascular permeability responses to intradermal serotonin in rats were showed significant effect at all sample groups. 2. The homologous passive cutaneous anaphylaxis in rats provoked by the IgE-like antibody aganist egg white albumin showed the decreasing effect. 3. The delayed type hypersensitivity responses to picryl chloride in mice were showed significant effect at all sample groups. 4. The delayed type hypersensitivity response to sheep red blood cell in mice were showed significant effect at all sample groups. 5. In anti-pyretic effect by yeast method were showed significant effect at all sample groups. 6. The anti-inflammatory effect by carrageenin method were showed significant effect at all sample groups. 7. The analgesic action by acetic acid method in mice were showed significant effect at all sample groups. According to the above result, Yenhwagamchotang(sample A), Yenhwagamchotang plus Forsythia koreana NAKAI(sample B), AND yenhwagamchotang plus Taraxacum mongolicum HNAN- MAZZ.(sample C ) were concluded to have the anti-allergic reaction, the antipyretic action, the anti-inflammatory, the analgesic action.

• YAKHAK HOEJI
• /
• v.36 no.4
• /
• pp.303-314
• /
• 1992

Experiments were performed on mice to investigate the effect of methionine diets (MET) on the immunotoxicity of ethanol. ICR female mice were divided into 5 groups, Met (Basal (B)+0.19% methionine(M), B+1.71% M and B+5.13%W) and ethanol(4%) were administered ad libitum for 21 days. The mice were evaluated for changes in immune status as measured by antibody titer, Arthus reaction, delayed type hypersensitivity (DTH), rosette forming cell(RFC) and plaque forming cell (PFC) to sheep red blood cells (S-RBC). To investigate the change of the non-specific immune response, the number of leukocytes in peripheral blood and phagocyte activity were measured. The results were summarized as follows: (1) The weight ratios of spleen and thymus to body weight were significantly increased by the B+0.19% M, B+0.57% M and B+1.71% M groups in comparison with control group(B), but B+5.13% M group was significantly decreased. (2) Humoral immune responses were significantly increased by the B+0.19% M and B+0.57% M groups in comparison with control group, but B+5.13% M group was significantly decreased. (3) Cellular immune responses were significantly decreased by the B+1.71% M and B+5.13% M groups in comparison with control group. (4) Phagocyte activities were significantly increased by the B+0.19% M, B+0.57% M and B+1.71% M groups in comparison with control groups, but B+5.13% M group was significantly decreased. (5) The number of circulating leukocyte was significantly increased in the B+0.19% M and B+0.57% M groups in comparison with control group, but B+5.13% M group was significantly decreased.

• The Journal of the Korean Society for Microbiology
• /
• v.18 no.1
• /
• pp.87-90
• /
• 1983

The immunosuppressive effect of serum from mice heavily infected with BCG(BCG-serum) was evaluated. The delayed-type hypersensitivity reaction to sheep erythrocytes was depressed when BCG-serum was administered both systemically and locally at the same time of challenge. This study shows that serum of animal infected with mycobacteria contains a factor responsible for immunosuppression and factor may be important in understanding the mechanisms of allergy in mycobacteria] infections.

• The Journal of Pediatrics of Korean Medicine
• /
• v.11 no.1
• /
• pp.249-273
• /
• 1997

Hyunggyeyungyotang has been used for treatment of sinusitis and otitis media in oriental medicine since ancient times. It is reported that Hyunggyeyungyotang has good effects on inflammatory and allergic diseases of otorhinolaryngology in clinical medicine. Kamihyunggyeyungyotang was made by adding several herbs to Hyunggyeyungyotang which has such good effects. To investigate the effects of Hyunggyeyungyotang and Kamihyunggyeyungyotang on inflammatory, algesic and allergic diseases, the author examined the analgesic effect by acetic acid reaction, studied the anti-inflammatory effect through the experiments of the protein thermo-denaturation and circumscribed edema. Besides researched the anti-allergic effect through the vascular permeability response to Chemical Mediator and the delayed type hypersensitivity response to Picryl Chloride. The obtained results were as follows ; 1. In the analgesic effect of Hyunggyeyungyotang and Kamihyunggyeyungyotang extract by acetic acid method, both of the sample groups showed the analgesia, but didn't show useful effect. 2. In the anti-inflammatory effect on the protein thermo-denaturation, the sample groups revealed the inhibitory effect in proportion to concentration as compared with the control group. 3. In the inhibitory action on circumscribed edema induced by Caraggeenin, both of Hyunggyeyungyotang and Kamihyunggyeyungyotang administration showed the significant effect after 4 hours in comparison to the control group. 4. In the delayed type hypersensitivity response to Picryl Chloride, both of the sample groups revealed the significant effects. 5. Both of the sample groups decreased the vascular permeability induced by Histamine in comparison with the control group, but the significancy was admitted in only Hyunggyeyungyotang administration. According to above results, Hyunggyeyungyotang and Kamihyunggyeyungyotang are considered to be used for treament of the inflammatory diseases including sinusitis.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.16 no.1
• /
• pp.165-171
• /
• 2002

The purpose of this research was to investigate effects of Hyunsamchungpye-eum(HSCPE) on the anti-inflammatory/anti-allergic reaction in vivo and in vitro. HSCPE reduced the acute hind paw edema induced by histamine, the permeability of evans blue into peritoneal cavity. HSCPE inhibited the passive cutaneous anaphylaxis reaction in rat, the lethal anaphylaxis and degranulation of peritoneal mast cells induced by compound 48/80 in mice, HSCPE did not affect the Arthus reaction, but decreased the delayed type hypersensitivity induced by SRBC, contact dermititis induced by DNFB. These results suggest that HSCPE have an anti-inflammatory and anti-allergic action.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.16 no.5
• /
• pp.1009-1015
• /
• 2002

The purpose of this research was to investigate the effect of Yonggak-san (YGS) on the anti-allergic reaction in vivo and in vitro. Administration of YGS(500 mg/kg) enhanced hemaggutination(HA)titer against SRBC. On the while, YGS inhibited hyaluronidase activity in vitro and passive cutaneous anaphylaxis reaction, lethal anaphylaxis and mortality induced by compound 48/80 in mice, YGS decreased Arthus reaction, acute hind paw edema induced by histamine. But YGS did not affect delayed type hypersensitivity induced by SRBC. These results suggest that YGS have anti-allergic action

• Environmental Analysis Health and Toxicology
• /
• v.6 no.1_2
• /
• pp.25-38
• /
• 1991

The immunopotenciating effects of petroleum ether extract, ethanol extract and butanol fraction of panax ginseng on the immunotoxicity of Cimetidine were investigated in ICR mice. Immune responses were evaluated by antibody production, Arthus reaction, delayed type hypersensitivity (DTH), and rosette forming cell (RFC) in mice, sensitized and challenged with sheep red blood cells. To investigate the change of the non-specific immune responses, phagocyte activity and number of leukocytes in peripheral blood were measured also. The results of this study are summarized as followings; 1. Cimetidine treated group as compared with normal group generally decreased HA, 2-MER, RFC, number of circulating leukocytes and phagocyte activity whereas in-creased Arthus reaction and DTH. 2. The panax ginseng petroleum ether extract combined administration group as compared with the control group remarkably increased HA, 2-MER, number of circulating leukocytes and phagocyte activity. 3. The panax ginseng ethanol extract combined administration group as compared with the control group remarkably increased Arthus reaction, DTH, HA, RFC, number of circulating leukocytes and phagocyte activity. 4. The panax ginseng butanol fraction combined administration group as compared with the control group remarkably increased Arthus reaction, HA, 2-MER, RFC, number of circulating leukocytes and phagocyte activity.

• Environmental Analysis Health and Toxicology
• /
• v.6 no.1_2
• /
• pp.59-70
• /
• 1991

This study was performed to investigate the effect of olive oil diet on the immune response in ICR male mice. Experimental diets of 4 groups were fed ad libitum to the ICR male mice for 27 days. The results of this study were summarized as followings: 1. 10% Olive oil diet group as compared with the control diet group significantly decreased liver weight rate but significantly increased hemagglutination titer (HA), Arthus reaction, delayed type reaction (DTH), rosette forming cell (RFC), and phagocyte activity. 2. 20% Olive oil hypersensitivity diet group as compared with the control diet group significantly increased body weight gain, liver weight rate, and HA but significantly decreased Arthus reaction, DTH, RFC, phagocyte activity, and peripheral circulating white blood cell (WBC). 3. 30% Olive oil diet group as compared with the control diet group significantly increased liver weight rate but significantly decreased body weight gain, Arthus reaction, plaque forming cell (PFC), DTH, RFC, phagocyte activity, and WBC. The results showed that the increase of olive oil doses significantly decreased humoral and cellular immune responses, phagocyte activity, and WBC.

• The Journal of the Korean Society for Microbiology
• /
• v.13 no.1
• /
• pp.17-24
• /
• 1978

Modulating effects of Candida albicans on the immune responses of mice immunized with sheep red blood cells(SRBC) were assessed both by footpad tests for anaphylactic, Arthus and delayed type hypersensitivity rections against homologous and heterologous antigenic challenges and by serum antibody titrations for hemagglutinin and hemolysin against SRBC. The results are summarized as follows: 1. In the mice simultaneously immunzed with C. albicans and SRBC, anaphylactic type and Arthus type footpad reactions to C. albicans challenge were enhanced, and extents of the enhancements were proportional to the concentration of SRBC administered for immunization, reaching peak in mice immunized with 0.2ml($10^8$) of 5% SRBC suspension. Although a little enhancement of delayed type hypersensitivity to C. albicans was observed in those mice, there was no significant difference between the mice groups immunized either with SRBC alone or SRBC and C. albicans simultaneously. 2. Simultaneous immunization of mice with C. albicans and SRBC resulted in the suppression of both anaphylactic type and Arthus type footpad reactions to SRBC, and the extent of such suppressions was inversly proportional to the numbers of C. albicans administered for immunization. Delayed type reaction of the mice to SRBC varied little in regards to the different numbers of C. albicans injected. 3. Hemagglutinin titers differed little between the mice groups immunized with SRBC alone or with SRBC and C. albicans simultaneously. Hewever, hemolysin titers were lower in the mice immunized simultaneously with SRBC and C. albicans. 4. In the peripheral blood of mice immunized simultaneously with SRBC and C. albicnas. there observed increases in the percents of monocyte and polymorphonuclear leukocytes and decrease in the numbers of lymphocytes and pyroninophilic lymphocytes. These results indicated that C. albicans is an immunosuppressant of the mice to SRBC when both anteigns were administered simultaneously for immunization, and that SRBC acted as an enhancer of anaphylactic type and Arthus type reaction of mice to C. albicans when administered simultaneously.