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Simple Mass-screening Methods for Resistance of Tomato to Fusarium oxysporum f. sp. lycopersici (토마토 시들음병에 대한 간편 대량 저항성 검정법)

  • Park, Myung Soo;Jang, Kyoung Soo;Choi, Yong Ho;Kim, Jin-Cheol;Choi, Gyung Ja
    • Horticultural Science & Technology
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    • v.31 no.1
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    • pp.110-116
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    • 2013
  • This study was carried out to establish the simple mass-screening methods for resistant tomato to Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici (FOL). Root dip inoculation method has been used in many studies on the resistance of tomato to disease. On the other hand, in mass-screening for resistant tomato to Fusarium wilt, the inoculation method is time-consuming and laborious procedure. Disease development of two FOL isolates on two cultivars of tomato according to inoculation method including root dip, tip and scalpel methods were investigated. In compatible interaction, tomato seedlings of each cultivar inoculated by tip method showed the lower and more variable disease severities than by root dip method. Whereas the seedlings by scalpel method represented clear resistant and susceptible responses to Fusarium wilt as root dip method. The resistance degree of each cultivar inoculated with FOL isolates by scalpel method was hardly affected by the tested incubation temperature and inoculum concentration. On the basis of the results, we suggest scalpel inoculation method as an efficient mass-screening method for resistant of tomato cultivars to Fusarium wilt. Roots of tomato seedlings at two-leaf stage grown in plastic cell tray were injured with scalpel and then spore suspension (more than $1{\times}10^7\;conidia{\cdot}mL^{-1}$) of FOL was poured directly on the roots. The infected plants were cultivated in a growth room at $25-30^{\circ}C$ for 4 weeks with 12-hours light a day.

The Effect of Korean Medicine Treatment on Cerebral Infarction with Hemiplegia: A Case Report (아급성기 뇌경색 환자의 좌측 편마비에 대한 한의 치료 1례)

  • Oh, Ju-hyun;Sung, Jae-yeon;Seo, Hye-jin;Lee, Yu-ra;Song, Jin-young;Kong, Geon-sik;Kang, Man-ho;Lee, Hyung-chul;Eom, Guk-hyeon;Song, Woo-sub
    • The Journal of Internal Korean Medicine
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    • v.40 no.5
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    • pp.990-998
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    • 2019
  • Introduction: Stroke refers to a sudden brain disease that results in disorders in the anatomy of the brain. The cause is a sudden circulatory disorder of the cerebrovascular system that creates a consciousness disorder and hemiplegia. Despite aggressive treatment after the onset, stroke is a social problem because the patient has difficulty in recovering from sequelae that can include limb movement disorders, language disorders, and emotional disorders. In this study, we describe the effect of traditional Korean medicine treatment on the sequelae in a subacute cerebral infarction patient admitted to a Korean medical hospital. Case presentation: A 67-year-old male patient was diagnosed with cerebral infarction circa 2005, and his condition had not improved. Around March 15, 2018, he experienced the sudden onset of a cerebral infarction in his daily life. This was confirmed by a brain MRI, and he was hospitalized at other hospitals, but he showed no improvement. We conducted a manual muscle test (MMT) to evaluate the patient's exercise and strength. His gait level was measured to evaluate his degree of walking. He was treated with Bojungikgi-tang and acupuncture twice a day. After 34 days of inpatient treatment, the patient's exercise strength improved from Grade 2+~Grade 3 to Grade 3+ determined by the MMT, and his walking ability improved from Gait Level 3 to Gait Level 4. Conclusion: The findings of this study indicate that acupuncture and herbal medicine treatment can help treat patients with hemiplegia due to cerebral infarction.

Effect of Non-paralyzed side and Paralyzed side of Elastic Band Combined with Proprioceptive Neuromuscular Facilitation Lower Extremity Patterns on Balance in Chronic Stroke Patients (비 마비 측과 마비 측에 적용한 탄력밴드를 결합한 고유수용성 신경근 촉진법 하지패턴이 만성 뇌졸중 환자의 균형에 미치는 영향)

  • Lee, Young-Min;Ham, Min-Sik;Kim, Taek-Soo
    • PNF and Movement
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    • v.13 no.4
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    • pp.181-188
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    • 2015
  • Purpose: This study aimed to examine the effects of proprioceptive neuromuscular facilitation (PNF) lower extremity patterns combined with elastic bands applied to stroke patients diagnosed with hemiplegia through self-training using the non-paralyzed side approach and the paralyzed side approach, and to investigate the differences in the effects. Methods: Nine chronic stroke patients who were being treated not more than twice a week at H, K, R, and C hospitals located in Gangwon-do, performed self-training for 16 minutes, two times per day for four weeks between August and October 2015. The subjects' balance ability was measured using the Tetrax stability index, the weight distribution index, the Berg Balance Scale (BBS), and the Timed Up and Go (TUG) test before the experiment and four weeks later. Among the statistical methods, paired t-tests were conducted for intra-group comparison of the measurements taken before and after the experiment, and independent t-tests were conducted for inter-group comparison of the ex post facto values. The statistical significance level was set to 0.05. Results: When the lower extremity patterns were applied to the non-paralyzed side group and the paralyzed side group, significant intra-group differences were observed for the Tetrax stability index, the weight distribution index, the Berg Balance Scale (BBS), and Timed Up and Go (TUG) tests (p<0.05); however, the inter-group comparisons showed no significant differences. Conclusion: The non-paralyzed side approach was found to be easy for patients to participate in and it also affected the patients' paralyzed side. Although the paralyzed side approach produced good exercise effects in a short period of time, it could result in adverse effects, such as a decrease in motivation and self-confidence. Therefore, these approaches are considered to be more effective when they are selectively applied depending on the purpose of the intervention and the degree of a patients' participation.

Effect of MSE on Activity and Molecular Biology in Rat Induced Liver Injury (간 손상으로 유도된 랫드에서 MSE가 활성 변화와 분자생물학에 미치는 영향)

  • Yea, Chun-Jung;Lee, Tae-Jong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.2
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    • pp.520-526
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    • 2019
  • The purpose of this experiment is to investigate the feasibility of extracts (sample extract, MSE) mixed with hot - water extracts as health functional nutritional foods for prevention and treatment of liver damage. The experimental method was to investigate the effects of carbon tetrachloride on liver damage in rats by 60% of liver cirrhosis level. The effect of mixed extracts (MSE) was investigated by measuring the physiological functions, biochemical functions, and molecular biologic TGF - ${\beta}$ and P53 levels in liver - injured rats. with MSE doses administered differently(0.50ml, 0.75ml). A total of 30 rats were used for normal group, control group, positive control group, experimental group 1, and experimental group 2. The rats were given 6 times a week for 6 weeks at a fixed time once a day. The results of the experiment were statistically significantly higher in the control group than in the normal group and the positive control group, and statistically significantly lower in the experimental group (1 and 2) than the control group. There were some significant differences in the comparison between the experimental groups (1 and 2), but no significant changes were found. In conclusion, the results of this study are expected to be useful as functional food, and the variation of physiological activity and the degree of liver molecular biotechnology will be used for the research of the same field.

Deokhu: Differences of investment activity and flow level according to self-perception, emotional change before-after deokhu activity (덕후: 자기 지각에 따른 투자 활동과 몰입 수준 차이, 덕후 활동 전후의 정서 변화)

  • KIM, SU-SIE;Lee, Heung-Pyo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.1
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    • pp.75-84
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    • 2019
  • The purpose of this study was to investigate whether there is a difference between the deokhu activities and the flow level of the deokhu group. We also tested whether there are any real differences in emotion before and after the deokhu activities. The study included 227 adults and results were as follows. First, the deokhu group had a high cost of investing and a high rate of one-day investments, particularly for one-week activities and years of duration compare to the non-deokhu group. Second, the higher the degree to which they perceived themselves as deokhu and the level of deokhu activities were higher in flow. and the group of deokhu was more absorbed than the non-deokhu group. Third, there has been no change in positive and negative feelings before and after deokhu activities in the non-deokhu group. For the deokhu group, the positive emotions of happiness, joy, tranquility, pleasure increased and negative emotions such as anger, anxiety, sadness, shame, and guilt were significantly reduced after deokhu activities. The perception of deokhu and deokhu activities have positive functions that increase the level of flow, relieve negative feelings and enhance positive emotions.

An Outlook of Changes in the Flowering Dates and Low Temperature after Flowering under the RCP8.5 Projected Climate Condition (기후변화 시나리오에 근거한 과수 개화기 변화 및 개화 후 저온 발생 전망)

  • Kim, Dae-jun;Kim, Jin-Hee
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.20 no.4
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    • pp.313-320
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    • 2018
  • In the spring of 2018, opened-flowers of fruit trees were frozen to death due to abnormal low temperature around Jeonbuk Province and southern Gyeonggi Province areas. In the 2000s, abnormal weather is observed all over the world very frequently. As a consequence, various sectors of the society suffer from economic damage and negative effects of the abnormal weather. Moreover, recent global climate change is believed to increase the incidence of extreme weathers, which are out of the normal range of the local climate. It is necessary to identify these abnormal weather phenomena accurately and analyze the effects of them on crops in order to understand the effects of them on crop yields. This study projected the trend of the low-temperature occurrence in the future by predicting the changes in future flowering dates and quantifying the temperature distribution after flowering using climate change scenarios. This study targeted areas actually producing a major portion of pear, peach, and apple in South Korea. The results of this study predicted that the flowering dates of these fruits will be approximately 20 days earlier than the current normal year in the future (2071-2100) for the study area. Moreover, it was found that the distribution of low temperature would vary by fruit type and region to some degree. The results of this study present only a portion of fruit trees cultivars grown in South Korea. It was expected that, when this approach is applied to various crops and fruit trees, it will be possible to contribute to preparing countermeasures for climate change in the agricultural sector.

The Effects of Childcare Teaching Teacher Efficacy and Psychological Burnout on The Practice of Childcare Respecting Rights for Infants (보육교사의 교사효능감과 심리적 소진이 영유아 권리존중보육 실행에 미치는 영향)

  • Ko, Young Yun;Shin, Hyun Jung
    • Korean Journal of Child Education & Care
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    • v.18 no.4
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    • pp.27-38
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    • 2018
  • Objective: The purpose of this study is to investigate the effects of childcare teachers' teacher efficacy, psychological burnout and the practice of respecting rights for infants. Methods: For this study, questionnaires were distributed to 398 childcare teachers in the cities of I and S. The collected data were analyzed by descriptive statistic, multiple regression and Cronbach's ${\alpha}$ for reliability by SPSS-WIN computer program. Results: The results yielded some interesting results. First, childcare teachers were shown to have higher respecting rights for infants but just average teacher efficacy rates. Secondly, the study showed a positive correlation between the practice of respecting rights for infants and childcare teachers' teacher efficacy. However, there was a negative correlation between the practice of respecting rights for infants and psychological burnout. There was also a negative correlation between the practice of respecting rights for infants and childcare teachers' teacher efficacy. Third, the higher the childcare teachers' teacher efficacy of childcare teachers, the more positive influences existed in the practice of respecting rights for infants. It also showed that the lower the degree of psychological burnout, there was a negative impact on the practice of respecting rights for infants. Conclusion/Implications: The results of this study suggest that a support plan is needed to boost childcare teachers' teacher efficacy, and to lower the psychological burnout of childcare teachers with regards to the practice of respecting rights for infants.

A non-invasive sexing method reveals the patterns of sex-specific incubation behavior in Saunders's Gulls (Saundersilarus saundersi) (비침습적 성감별 방법에 의한 검은머리갈매기(Saundersilarus saundersi)의 암수 포란행동)

  • Joo, Eun-Jin;Ha, Mi-Ra;Jeong, Gilsang;Yoon, Jongmin
    • Korean Journal of Ornithology
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    • v.25 no.2
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    • pp.69-76
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    • 2018
  • Sexual dimorphism in birds refers to male-female differences in body size, plumage, color and/or behavior. In general, many seabirds, including the family of Laridae, are monomorphic in plumage-color, which makes the determination of sex difficult in the field because both parents also tend to share a great portion of parental care. The development of an inexpensive sexing tool facilitates understanding the degree of sex-specific parental care in the evolution of the life history. Here, we developed a non-invasive method for the determination of sex using the bill-head morphometric of known captive pairs and applied this tool to wild pairs to document factors underlying male-female parental care during the incubation period of Saunders's gulls (Saundersilarus saundersi). Males exhibited relatively larger bill-head ratios than their mates within naturally formed pairs in captivity, resulting in the determination of sex in12 wild pairs at the nest during the incubation period. Males and females equally shared the incubation role during the daytime, attending the nest at a high rate of 95%. However, the male's proportion of nest attentiveness greatly increased with time towards sunset, presumably reflecting the male duty for nighttime incubation. The present study provides a non-invasive method for the determination of sex in a monomorphic seagull species and highlights how male-female incubation behavior is associated with time of the day, rather than other ecological conditions.

A Study on Self-reliance and Residential Service Needs of Patients with Chronic Mental Illness (만성정신질환자의 자립 및 주거 서비스에 대한 요구도 조사)

  • Jeon, Hyun Ju;Huh, Yu Jeong;Ko, Young-Hoon;Lee, Jae-Hon
    • Korean Journal of Psychosomatic Medicine
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    • v.26 no.2
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    • pp.145-151
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    • 2018
  • Objectives : Residential services are provided to help patients with chronic mental illness, but those have some problems because of provider focused services and uniformized training. So the purpose of the study was to explore residential needs of patients with the chronic mental illness such as chronic schizophrenia. Methods : A survey was conducted on 139 mental illness patients using mental health facilities at Ansan city area in South Korea. We investigated their demographic and social characteristics, the degree of self- reliance and residential service needs. Results : More than half of them had the desire for self-reliance, and they needed job support mostly. They were positive about the use of residential facilities and needed daily living skill and social skill training. Also they preferred day rehabilitation in type of facility. And they wanted to operate in a way that they returned home at the weekend after group home. Conclusions : For facilitating the recovery of patients with chronic mental illness, it would be necessary to provide individualized residential rehabilitation services and to improve existing residential facilities and programs reflected on the patients' needs.

The effect of different culture conditions of liquid spawn on the quality characteristics of shiitake mushroom (Lentinula edodes) (표고의 액체종균 배양 조건 및 품질 특성)

  • Lee, Soo-Jung;Kim, Hun-Hwan;Kim, Seon-Ho;Kim, Sung-Hee;Sung, Nak-Ju
    • Journal of Mushroom
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    • v.17 no.3
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    • pp.99-106
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    • 2019
  • To improve the productivity of shiitake mushroom (Lentinula edodes), seven different types of media for liquid spawn (denoted as "A" to "G") were prepared with 0.3% soybean meal and varying sugar and glucose concentrations. During 14 days of incubation, the pH of the liquid culture gradually acidified with increasing incubation period. Additionally, there was a significant, but not prominent, difference in the degree of acidification depending on the sugar to glucose ratio. Liquid spawn culture "G," which had the highest sugar content was the most acidic on the last day of incubation. Mycelium dry weight increased significantly with increasing incubation period, and there was no significant difference in mycelium dry weight irrespective of the sugar to glucose ratio even after 14 days of culture. The inoculation of liquid spawn in sawdust medium with an inoculation volume ${\geq}45mL$ and incubation period of 15 to 18 days were the optimal culture conditions. Productivity of fruit bodies in sawdust medium and mushrooms treated with liquid spawn was significantly higher compared to solid spawn treatment. The mushrooms treated with liquid spawn had better chewiness, and the free amino acid content, which is associated with savory taste, was higher in these mushrooms compared to those treated with solid spawn.