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Clinical characterization of 3-month-old pigs infected with African swine fever virus from Vietnam

  • Oh, Sang-Ik;Bui, Vuong Nghia;Dao, Duy Tung;Bui, Ngoc Anh;Yi, Seung-Won;Kim, Eunju;Lee, Han Gyu;Bok, Eun-Yeong;Wimalasena, S.H.M.P;Jung, Young-Hun;Hur, Tai-Young;Lee, Hu Suk
    • Korean Journal of Veterinary Service
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    • v.45 no.2
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    • pp.71-77
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    • 2022
  • African swine fever (ASF) is a fatal viral disease in pigs, with a short incubation period and causing immediate death. Few studies exist on the Asian epidemic ASF virus (ASFV) challenge in older pigs, including growing and fattening pigs and sows. We aimed to investigate clinical outcomes, pathomorphological lesions, and viral distribution in organs of 3-month-old growing pigs that were inoculated with the ASFV isolated in Vietnam. The clinical outcomes were recorded daily, and the dead or euthanized pigs immediately underwent necropsy. Viral loads were determined in 10 major organs using quantitative polymerase chain reaction. The average incubation period in growing pigs was more delayed (5.2±0.9 dpi) than that in weaned pigs, and the clinical signs were milder in growing pigs than in weaned pigs. The digestive and respiratory clinical signs in growing pigs showed at the end period of life, but these were observed at an early stage of infection in weaned pigs. The pathomorphological features were severe and nonspecific with hemorrhagic lesions in various organs. The viral loads in organs from growing pigs were higher than those from piglets, and the number of viral copies was related to gross lesions in the tonsil and intestine. In the absence of vaccines against ASF, early clinical detection is important for preventing the spread of the virus. Our findings elucidated that the clinical signs and gross lesions in growing pigs differed from those in weaned pigs, which provide valuable information for diagnosis of pigs with suspected ASF infection.

Researching the Occurrence Potential of Autoite for Living Modified OrganismMaize Spill (유전자변형생물체 옥수수의 자생개체 발생가능성 조사)

  • Eom, Gyu-Hyeon;Jang, Yoon-Hee;Du, Xiao-Xuan;Kim, Eun-Gyeong;Park, Jae-Ryoung;Ryu, Taehun;Kim, Kyung-Min
    • Journal of Life Science
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    • v.32 no.8
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    • pp.641-646
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    • 2022
  • There are concerns about the environmental release of living modified organism (LMO) maize created to increase yields. In fact, there are cases in which LMO crops for feed have been leaked in Korea to form autoite colonies, and concerns about LMO spill are intensifying. In this study, the possibility of environmental outflow and occurrence of native organisms was analyzed using maize feed and seeds distributed in Korea. In the evaluation of the possibility of spontaneous occurrence of maize in the event of an unintentional release of maize feed made by crushing maize, the incidence rate of maize was 0.01%, which was extremely low compared to the germination rate of maize seeds. A survey of the dormant rate of maize showed that all maize seeds collected every month were dead. In the germination rate test by temperature using Daehak wax corn and Kwangpyeongok, high germination rates were found at 20℃ and 30℃, and relatively low germination rates were found at 10℃ and 40℃. In addition, all germination tests showed a higher germination rate Daehak wax corn than Kwangpyeongok. The difference between domestic and overseas cultivation maize was confirmed through a survey on the agricultural properties of three varieties of maize. The data obtained through this experiment could be the basis for the evaluation of the weediness potential of environmental risk assessment and technology to suppress the occurrence of autoite in preparation for future LMO spills.

Sorting of Aged Seeds Using Leakage Substances of Crop Seeds (종자 누출물질을 이용한 노화종자의 선별)

  • Lee, Dong-Jin;Lim, Moo-Sang;Chu, Young-Ha;Chung, Kil-Woong;Kim, Bong-Ku
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.6
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    • pp.783-789
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    • 1997
  • This study was conducted to identify leakage of fluorescent substances out of 56 crops and to develop sorting methods of nonviable seeds of Chinese cabbage. Fluorescent substances were detected from 36 species, Chinese cabbage, radish, broccoli, cauliflower, egg plant and rape seed etc. In Chinese cabbage, the germination rate of non-sorted seeds decreased rapidly with ageing of seeds at 45$^{\circ}C$ and 100%. After Chinese cabbage seeds soaked for 4hrs and coated with cellulose, the sorting ratio of non fluorescent seeds were decreased 91.3% to 1.7% by increasing ageing periods from control to 8 days, while fluorescent seeds were increased drastically from 8.7% to 98.3%. Also, incubation of water soaked dead seeds of Chinese cabbage treated with 0.5-1.0% ninhydrin and 0.01N sodium thiosulfate for 30~60 minutes at 35$^{\circ}C$ developed purple color.

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A Study on 3D Virtual Restoration and Convergence Utilization of Gas Masks for Digital Reproduction of War Cultural Heritage (전쟁 문화유산 디지털 재현을 위한 방독면 3D 가상 복원 및 융합 활용 연구)

  • Hyoung-Ki Ahn;Seung-Jun Oh;Ho-Yeon Lee;Young-Guy Lee
    • Journal of Internet of Things and Convergence
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    • v.9 no.1
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    • pp.89-95
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    • 2023
  • In January 2007, the Remains Excavation and Investigation Team of the Ministry of National Defense was established, and full-scale excavation of remains was promoted. Currently, the scope of the excavation is being expanded to Baekma Hill within the DMZ, where fierce battles were fought during the Korean War. Now, many remains and remains are being excavated in Baekma hill. Most are in damaged condition. Therefore, in this study, the original form of the excavated remains was restored using 3D scanning and 3D modeling. This digital restoration method can be an alternative to compensate for the disadvantages of the manual method. Currently, various digital restorations using 3D technology are active in the field of cultural heritage. Digitally restored materials can be used as basic data for digital heritage. Based on this, various contents related to excavation of remains and patriots and veterans can be developed. Furthermore, if digital human restoration is made based on the excavated remains, it will be possible to reproduce the appearance of the dead.

Research on Artistic Artmask for Lifecaretainment (라이프케어테인먼트를 위한 예술적 아트마스크 연구)

  • Yoon, Hee
    • Journal of Korea Entertainment Industry Association
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    • v.13 no.3
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    • pp.269-278
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    • 2019
  • This study is intended to present the direction of art mask design development and help people's life care by producing an art mask with the motif of the characteristic of abjection concept. It went through the definition and characteristics of abject and abjection focusing on previous research, professional books and art works. The characteristics of abjection based on theoretical research are to express death with dead bodies and skulls, including the dismantling of ideal beauty standards, the expression of fragmented bodies, and the expression of sick and alienated bodies. This researcher produced 6 research works by combining the fascinating abjection, induced by ugly and disgusting abject, to design factors. These works fragmented each part of the facial form by the cutting and dismantling, and made viewers feel the abjection by giving a grotesque deformation. The results obtained through the research works are as follows. First, It was found that the abjection characteristics-applied works was helpful in the treatment of the mental wound of modern people. Second, the production of works based on the abjection characteristics paved the way to expand the scope of art mask design concepts and establish the new design ideas. Third, the application of the motif using the abjection characteristics to the art mask design showed that the use of various objects could express creative designs and secure diversity in using materials. In the future, this researcher hopes that such research will be used as the basic data of the follow-up study on art mask design, and it will help develop art mask designs.

Evaluation of Insecticidal Efficacy of Six Eco-friendly Agricultural Materials and Metarhizium anisopliae against Ramulus mikado (대벌레(Ramulus mikado)에 대한 유기농업자재 6종과 녹강균(Metarhizium anisopliae)의 살충 효과 평가)

  • Jong-Kook Jung;Bok-Nam Jung;Cha Young Lee;Keonhee E. Kim;Junheon Kim;Young Su Lee;Ji-Hyun Park
    • Journal of Korean Society of Forest Science
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    • v.112 no.1
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    • pp.117-125
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    • 2023
  • Outbreaks of Ramulus mikado (Insecta: Phasmatodea: Phasmatidae) in the hilly areas of Mt. Bongsan, Mt. Cheonggye, and elsewhere in Seoul and Gyeonggi occurred from 2020 to 2021, causing serious defoliation. We evaluated the insecticidal effects of six eco-friendly organic materials and the insect-pathogenic fungus Metarhizium anisopliae against R. mikado. The fungus was isolated from naturally dead bodies of R. mikado in forest ecosystems. The results revealed that three eco-friendly organic materials containing azadirachtin or geraniol as active ingredients showed high mortality in the range of 85.2%-100%, which were rates similar to that of the chemical insecticide fenitrothion emulsifiable concentrate. All R. mikado adults that were sprayed with a conidial suspension of M. anisopliae at different concentrations were killed within a few days. In conclusion, three eco-friendly organic materials and M. anisopliae could be good alternatives to chemical insecticides.

Determination of Resistance Factors of Load and Resistance Factor Design for Drilled Shaft Based on Load Test (LRFD 설계를 위한 현장타설말뚝의 주면지지력 저항계수 산정)

  • Kim, Seok-Jung;Kwon, Oh-Sung;Jung, Sung-Jun;Han, Jin-Tae;Kim, Myoung-Mo
    • Journal of the Korean Geotechnical Society
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    • v.26 no.7
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    • pp.17-24
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    • 2010
  • Load Resistance Factor Design method is used increasingly in geotechnical design world widely and resistance factors for drilled shafts are suggested by AASHTO. However, these resistance factors are determined for intact rock conditions; by comparison, most of bedrocks in Korea have weathered condition, so that applying the AASHTO resistance factors is not reasonable. Thus, this study suggests the proper resistance factors for design of drilled shaft in Korea. The 22 cases of pile load test data from 8 sites were chosen and reliability-based approach is used to analyze the data. Reliability analysis was performed by First Order Second Moment Method (FOSM) applying 4 bearing capacity equations. As a result, when the Factor of Safety (FOS) was selected as 3.0, the target reliability indexes (${\beta}_c$) were evaluated as 2.01~2.30. Resistance factors and load factors are determined from optimization based on above results. The resistance factors ranged between 0.48 and 0.56 and load factors for dead load and live load are evaluated as approximately 1.25 and 1.75 respectively. However, when the target reliabilities are considered as 3.0, the resistance factors are evaluated as approximately 50% of the results when the target reliability index was 2.0.

Assessment of the resistance of bacterial soft rot caused by Pectobacterium carotovorum subsp. carotovorum KACC 21701 in Kimchi cabbage genetic resources

  • Parthiban Subramanian;Ho Chul Ko;Seong-Hoon Kim;Jae Eun Lee;Aejin Hwang;Bichsaem Kim;Yoon-Jung Lee;Awraris Derbie Assefa;Onsook Hur;Nayoung Ro;Jung Sook Sung;Ju Hee Rhee;Ho-Sun Lee;Bum-Soo Hahn
    • Korean Journal of Environmental Biology
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    • v.40 no.4
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    • pp.433-441
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    • 2022
  • Bacterial phytopathogen Pectobacterium causes soft rot disease in several vegetable crops globally, resulting in heavy agricultural losses at both the pre and postharvest stages. The present work was carried out to screen Kimchi cabbage genetic resources conserved at the National Agrobiodiversity Center, Rural Development Administration, Korea, for resistance against the soft rot pathogen Pectobacterium carotovorum subsp. carotovorum KACC 21701 over a period of three years (from 2020 to 2022). Infection of the phytopathogen was carried out at four-leaf stage and for each accession, twenty-five plants per germplasm were infected with KACC 21701. Kimchi cabbage cultivars Wangmatbaechu, Seoulbaechu, and CR Kiyoshi were used as control. Seven-days post-infection, the Disease Index (DI) values were manually recorded from zero to four, zero matched perfectly heathy plants and four completely dead plants. The 682 accessions of Kimchi cabbage exhibited varying degrees of disease resistance to KACC 21701 and thirty accessions, exhibiting a DI≤2, were considered for replication studies. During the replication studies, four landrace germplasms (IT102883, IT120036, IT120044, and IT120048) and one cultivar(IT187919) were confirmed to be moderately susceptible to KACC 21701. Results of the preliminary screening as well as replication studies were documented for the all the 682 germplasms. Addition of such information to the passport data of stored germplasms might serve as potential bio-resource for future breeders and researchers to develop resistant varieties or study the mechanisms involved in resistance of plants to such phytopathogen.

Selection of Drought Tolerant Plants by Drought the Physiological Characteristics and Biochemicals Material about the Compositae Plants (건조 생리특성 및 생화학적 물질을 인자로 한 국화과 식물의 내건성 식물 선발)

  • Yang, Woo Hyeong;Im, Hyeon Jeong;Park, DongJin;Kim, Hak Gon;Yong, Seong Hyeon;Kang, Seung Mi;Ma, Ho Seop;Choi, Myung Suk
    • Journal of agriculture & life science
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    • v.50 no.5
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    • pp.51-60
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    • 2016
  • This study was selected drought tolerant plants, by observing the physiological characteristics and biochemical materials from the 9 kinds of the Compositae plants. After selecting plants of the similar size, and then drought stress was induced by the irrigation stopping. Survival rates, chlorophyll values, relative water content(RWC), excised-leaf water loss(ELWL), proline, reducing sugar were measured after 30 days of stopping irrigation. The species that had high rates of survival were Ainsliaea acerifolia Sch. Bip, Aster koraiensis, Aster scaber, Dendranthema zawadskii(S), however other 5 species were dead. The remaining factors have been determined based on plant species showed a higher survival rate. However, chlorophyll content showed high values in A. acerifolia, A.altaicus var. uchiyamae, A. koraiensis, and will have been determined that has no correlation with survival rates, except for A. acerifolia and A. koraiensis. On the other hand, A. scaber, A. acerifolia, A. koraiensis were determined to be relatively high drought tolerant plants in RWC, ELWL, proline, reducing sugar, it showed a similar correlation with survival rate. As a result of 9 kinds of the Compositae plants A. scaber, A. acerifolia, A. koraiensis were considered relatively higher drought tolerant plants.

A Study on the Development of Harmonic Limit Device for Stabilizing Main Circuit Equipment of Train (열차운행 안정화를 위한 주회로 기기의 고조파 제한장치 개발에 관한 연구)

  • Kim, Sung Joon;Chae, Eun Kyung;Kang, Jeong Won
    • Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
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    • v.8 no.6
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    • pp.853-861
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    • 2018
  • This paper proposes the application of harmonic constraints to address the problems caused by abnormal voltage increases when electric railway vehicles are running. The AC line that supplies the train with power during operation is used to provide electricity of 25kV/60 Hz, but gradually the size and frequency of harmonics involved in the line are varied with the technological evolution of the railroad vehicle electrical equipment. An increase in heat losses due to the failure of the instrument transformer (PT), the main circuit device, which is a serious problem with the recent train safety operation, or to the main displacement voltage. When high frequency components are introduced through low frequency Transformers of the main circuit device, the high intensity of the components is caused by the high intensity of the core and the current flow of the parasitic core is increased, thus generating heat. To solve this problem, the recent adjustment of the sequence has applied artificial NOTCH OFF of the power converter. However, the method of receiving and controlling the OFF signal operates by interaction between the ground and the vehicle's devices, thus it is invalid in the event of failure, and an actual accident is occurring. Therefore, the harmonic currents were required to prevent possible flow of harmonics, and conducted a study to prevent accidental occurrence of train accidents and to verify feasibility of the device through the simulations of the train's experimental analysis and the simulations of the train for safe operation.