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Human Papillomavirus Genotypes among Females in Mexico: a Study from the Mexican Institute for Social Security

  • Salcedo, Mauricio;Pina-Sanchez, Patricia;Vallejo-Ruiz, Veronica;Monroy-Garcia, Alberto;Aguilar-Lemarroy, Adriana;Cortes-Gutierrez, Elva I.;Santos-Lopez, Gerardo;Montoya-Fuentes, Hector;Grijalva, Renan;Madrid-Marina, Vicente;Apresa-Garcia, Teresa;Hernandez, Dulce M.;Jave-Suarez, Luis F.;Romero, Pablo;Poot, Albros;Salgado, Eduardo;Ramos-Gonzalez, Patricia;Gonzalez-Hernandez, Rigoberto;Canton, Juan C.;Jimenez-Aranda, Lucio;Parra-Melquiadez, Miriam;Paniagua, Lucero;Mendoza, Monica;Arreola, Hugo;Villegas, Vanesa;Torres-Poveda, Kirvis;Bahena-Roman, Margarita;Gonzalez-Yebra, Beatriz;Taniguchi, Keiko;Rodea, Carlos;Mantilla-Morales, Alejandra;Mora-Garcia, Maria L.;Velazquez-Velazquez, Cindy K.;Cordova-Uscanga, Candelaria;Peralta, Raul;Lopez-Romero, Ricardo;Marrero, Daniel;Bandala, Cindy;Reyes-Leyva, Julio;Furuya, Maria E.;Almeida, Eduardo;Galvan, Maria E.;Grijalva, Israel
    • Asian Pacific Journal of Cancer Prevention
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    • 제15권23호
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    • pp.10061-10066
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    • 2015
  • Background: The aetiological relationship between human papillomavirus (HPV) infection and cervical cancer (CC) is widely accepted. Our goal was to determine the prevalence of HPV types in Mexican women attending at the Mexican Institute for Social Security from different areas of Mexico. Materials and Methods: DNAs from 2,956 cervical samples were subjected to HPV genotyping: 1,020 samples with normal cytology, 931 with low-grade squamous intraepithelial lesions (LGSIL), 481 with high grade HGSIL and 524 CC. Results: Overall HPV prevalence was 67.1%. A total of 40 HPV types were found; HPV16 was detected in 39.4% of the HPV-positive samples followed by HPV18 at 7.5%, HPV31 at 7.1%, HPV59 at 4.9%, and HPV58 at 3.2%. HPV16 presented the highest prevalence both in women with altered or normal cytology and HPV 18 presented a minor prevalence as reported worldwide. The prevalence ratio (PR) was calculated for the HPV types. The analysis of PR showed that HPV16 presents the highest association with CC, HPV 31, -33, -45, -52 and -58 also demonstrating a high association. Conclusions: The most prevalent HPV types in cervical cancer samples were -16, -18, -31, but it is important to note that we obtained a minor prevalence of HPV18 as reported worldwide, and that HPV58 and -52 also were genotypes with an important prevalence in CC samples. Determination of HPV genotypes is very important in order to evaluate the impact of vaccine introduction and future cervical cancer prevention strategies.

On the bending and stability of nanowire using various HSDTs

  • Youcef, Djamel Ould;Kaci, Abdelhakim;Houari, Mohammed Sid Ahmed;Tounsi, Abdelouahed;Benzair, Abdelnour;Heireche, Houari
    • Advances in nano research
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    • 제3권4호
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    • pp.177-191
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    • 2015
  • In this article, various higher-order shear deformation theories (HSDTs) are developed for bending and buckling behaviors of nanowires including surface stress effects. The most important assumption used in different proposed beam theories is that the deflection consists of bending and shear components and thus the theories have the potential to be utilized for modeling of the surface stress influences on nanowires problems. Numerical results are illustrated to prove the difference between the response of the nanowires predicted by the classical and non-classical solutions which depends on the magnitudes of the surface elastic constants.

Optimization of Trichomonas vaginalis Diagnosis during Pregnancy at a University Hospital, Argentina

  • Testardini, Pamela;Vaulet, Maria Lucia Gallo;Entrocassi, Andrea Carolina;Menghi, Claudia;Eliseht, Martha Cora;Gatta, Claudia;Losada, Mirta;Touzon, Maria Sol;Corominas, Ana;Vay, Carlos;Tatti, Silvio;Famiglietti, Angela;Fermepin, Marcelo Rodriguez;Perazzi, Beatriz
    • Parasites, Hosts and Diseases
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    • 제54권2호
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    • pp.191-195
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    • 2016
  • The aim of this study was to evaluate different methods for Trichomonas vaginalis diagnosis during pregnancy in order to prevent maternal and perinatal complications. A total of 386 vaginal exudates from pregnant women were analyzed. T. vaginalis was investigated by 3 types of microscopic examinations direct wet mount with physiologic saline solution, prolonged May-Grunwald Giemsa (MGG) staining, and wet mount with sodium-acetate-formalin (SAF)/methylene blue method. PCR for 18S rRNA gene as well as culture in liquid medium were performed. The sensitivity and specificity of the microscopic examinations were evaluated considering the culture media positivity or the PCR techniques as gold standard. The frequency of T. vaginalis infection was 6.2% by culture and/or PCR, 5.2% by PCR, 4.7% by culture, 3.1% by SAF/methylene blue method and 2.8% by direct wet smear and prolonged MGG staining. The sensitivities were 83.3%, 75.0%, 50.0%, and 45.8% for PCR, culture, SAF/methylene blue method, and direct wet smear-prolonged MGG staining, respectively. The specificity was 100% for all the assessed methods. Microscopic examinations showed low sensitivity, mainly in asymptomatic pregnant patients. It is necessary to improve the detection of T. vaginalis using combined methods providing higher sensitivity, such as culture and PCR, mainly in asymptomatic pregnant patients, in order to prevent maternal and perinatal complications.

Screening and isolation of antibacterial proteinaceous compounds from flower tissues: Alternatives for treatment of healthcare-associated infections

  • de Almeida, Renato Goulart;Silva, Osmar Nascimento;de Souza Candido, Elizabete;Moreira, Joao Suender;Jojoa, Dianny Elizabeth Jimenez;Gomes, Diego Garces;de Souza Freire, Mirna;de Miranda Burgel, Pedro Henrique;de Oliveira, Nelson Gomes Junior;Valencia, Jorge William Arboleda;Franco, Octavio Luiz;Dias, Simoni Campos
    • 셀메드
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    • 제4권1호
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    • pp.5.1-5.8
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    • 2014
  • Healthcare-associated infection represents a frequent cause of mortality that increases hospital costs. Due to increasing microbial resistance to antibiotics, it is necessary to search for alternative therapies. Consequently, novel alternatives for the control of resistant microorganisms have been studied. Among them, plant antimicrobial protein presents enormous potential, with flowers being a new source of antimicrobial molecules. In this work, the antimicrobial activity of protein-rich fractions from flower tissues from 18 different species was evaluated against several human pathogenic bacteria. The results showed that protein-rich fractions of 12 species were able to control bacterial development. Due its broad inhibition spectrum and high antibacterial activity, the protein-rich fraction of Hibiscus rosa-sinensis was subjected to DEAE-Sepharose chromatography, yielding a retained fraction and a non-retained fraction. The retained fraction inhibits 29.5% of Klebsiella pneumoniae growth, and the non-retained fraction showed 31.5% of growth inhibition against the same bacteria. The protein profile of the chromatography fractions was analyzed by using SDS-PAGE, revealing the presence of two major protein bands in the retained fraction, of 20 and 15 kDa. The results indicate that medicinal plants have the biotechnological potential to increase knowledge about antimicrobial protein structure and action mechanisms, assisting in the rational design of antimicrobial compounds for the development of new antibiotic drugs.

Molecular Characterization of Trypanosoma cruzi Tc8.2 Gene Indicates Two Differential Locations for the Encoded Protein in Epimastigote and Trypomastigote Forms

  • Kian, Danielle;Lancheros, Cesar Armando Contreras;Damiani, Igor Alexandre Campos;Fernandes, Tamiris Zanforlin Olmos;Pinge-Filho, Phileno;dos Santos, Marcia Regina Machado;da Silveira, Jose Franco;Nakamura, Celso Vataru;da Silva, Joao Santana;Yamada-Ogatta, Sueli Fumie;Yamauchi, Lucy Megumi
    • Parasites, Hosts and Diseases
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    • 제53권4호
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    • pp.483-488
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    • 2015
  • This report describes the molecular characterization of the Tc8.2 gene of Trypanosoma cruzi. Both the Tc8.2 gene and its encoded protein were analyzed by bioinformatics, while Northern blot and RT-PCR were used for the transcripts. Besides, immunolocalization of recombinant protein was done by immunofluorescence and electron microscopy. Analysis indicated the presence of a single copy of Tc8.2 in the T. cruzi genome and 2-different sized transcripts in epimastigotes/amastigotes and trypomastigotes. Immunoblotting showed 70 and 80 kDa polypeptides in epimastigotes and trypomastigotes, respectively, and a differential pattern of immunolocalization. Overall, the results suggest that Tc8.2 is differentially expressed during the T. cruzi life cycle.

아르헨티나의 피케테로스 운동의 사회적 연대의 맥락연구 - 부르디외의 아비투스 개념을 중심으로 - (Estudio del contexto de solidaridad social de los movimientos Piqueteros en la Argentina)

  • 안태환
    • 이베로아메리카
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    • 제12권2호
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    • pp.189-216
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    • 2010
  • Después de los años 90, en la América Latina, nos llama la atención el surgimiento de los nuevos movimientos sociales que quieran superar o cambiar los caracteres del regimen neoliberalista, el cual nos obliga mantenernos aislados o dispersados. Sobre todo se trató así fuerte en la Argentina con los movimientos sociales de los desocupados pobres que se llaman 'piqueteros' en la segunda mitad de la década 90. En este estudio, he intentado de comprender y explicar cuáles motivos o contextos socio-culturales hayan movido esos movimientos sociales con la ayuda del marco teórico de 'habitus' de Pierre Bourdieu. Por ello, es muy importante interpretar la solidaridad entre la clase obrera y la media según la perspectiva de Bourdieu debido a la sugerencia de habitus de la clase obrera y la media latinoamericana basado en la cultura oral. Porque los movimientos piqueteros argentinos surgieron no desde la clase social ni del individuo abstracto-racional de la modernidad sino de los individuos reales. Y el concepto de habitus se penetra en el cuerpo de los individuos de manera inconsciente construido desde fuera de las condiciones histórico-sociales por mucho tiempo. Y también en la Argentina esa solidaridad ha desarrollado no sólo a superar la pobreza en sí misma sino a construir nuevas maneras sociales de los procesos de producción capitalista en forma del movimiento cooperativista, que se llama 'las empresas recuperadas por los trabajadores'. Por tanto, he analizado el proceso de cambio social en el ámbito de la América Latina relacionando el habitus con la estructura socio-cultural de la oralidad en dos sentidos; primero, se recalca la importancia de solidaridad entre las clases y la media segundo, fuerte resistencia social ante los crisis socio-económicos. Por ultimo, hay que valorar la importancia de la aparición de los nuevos sujetos sociales así como esos movimientos cooperativistas, socialización de los procesos de producción capitalista en renovar la democracia misma.

Transmission Fiber Chromatic Dispersion Dependence on Temperature: Implications on 40 Gb/s Performance

  • Andre, Paulo S.;Teixeira, Antonio L.;Pinto, Armando N.;Pellegrino, Lara P.;Neto, Berta B.;Rocha, Jose F.;Pinto, Joao L.;Monteiro, Paulo N.
    • ETRI Journal
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    • 제28권2호
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    • pp.257-259
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    • 2006
  • In this letter, we will evaluate the performance degradation of a 40 km high-speed (40 Gb/s) optical system, induced by optical fiber variations of the chromatic dispersion induced by temperature changes. The chromatic dispersion temperature sensitivity will be estimated based on the signal quality parameters.

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Experimental study of beam-column joints in axially loaded RC columns strengthened by steel angles and strips

  • Adam, Jose M.;Gimenez, Ester;Calderon, Pedro A.;Pallares, Francisco J.;Ivorra, Salvador
    • Steel and Composite Structures
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    • 제8권4호
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    • pp.329-342
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    • 2008
  • The strengthening of reinforced concrete (RC) columns by steel angles and strips (steel cage) is one of various techniques available to increase ultimate column load. Different authors have shown the influence of the beam-column joint on the behaviour of columns strengthened by steel cages. This paper presents an experimental study carried out at the Universidad Polit$\acute{e}$cnica de Valencia with the aim of analysing two different techniques to solve the strengthening close to the joint and the influence on the behaviour of RC columns strengthened steel cages. The ultimate loads obtained in the laboratory tests for these two techniques are compared to that specified by Eurocode 4.

MULTI-DIMENSIONAL APPROACHES IN SEVERE ACCIDENT MODELLING AND ANALYSES

  • Fichot, F.;Marchand, O.;Drai, P.;Chatelard, P.;Zabiego, M.;Fleurot, J.
    • Nuclear Engineering and Technology
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    • 제38권8호
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    • pp.733-752
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    • 2006
  • Severe accidents in PWRs are characterized by a continuously changing geometry of the core due to chemical reactions, melting and mechanical failure of the rods and other structures. These local variations of the porosity and other parameters lead to multi-dimensionnal flows and heat transfers. In this paper, a comprehensive set of multi-dimensionnal models describing heat transfers, thermal-hydraulics and melt relocation in a reactor vessel is presented. Those models are suitable for the core description during a severe accident transient. A series of applications at the reactor scale shows the benefits of using such models.