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Effects of Subatrates Supplemented with Bioceramic. Crushed Shell and Elvanite on the Growth of Watermelon, Cucumber and Tomato Seedlings. (바이오세라믹, 패화석 및 맥반석의 혼입처리가 수박, 오이 및 토마토의 유묘성장에 미치는 영향)

  • 박순기;김홍기;정순주
    • Korean Journal of Organic Agriculture
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    • v.6 no.1
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    • pp.109-116
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    • 1997
  • This experiment was carried out to examine the effect of various functional materials such as bioceramic podwers, crushed shells and elvanites supplemented to the each substrate on the seedlings growth of cucumber, watermelon and tomato. The seedlings were grown in pots filled with substrates of bioceramic podwers, crushed shell and elvanites. The growth of cucumber seedlings in terms of plant height, stem diameter, leaf width, leaf area, plant fresh and dry weight was promoted by adding the bioceramic. powder (1 to 2g/kg), crushed shells (20 to 80g/kg) or elvanites (20 to 80g/kg). Watermelon seedlings in terms of plant height, number of leaves and leaf area were greater than those of the control by adding bioceramics (1 to 2g/kg). Plant height was also promoted by the adding of bioceramic power from 16 days after treatment. But leaf area was increased from 8 days after treatment, while stem diameter was not affected. Watermelon seedlings were also influenced by adding curshed shells (20 to 80g/kg) and elvanites (20 to 40g/kg) into each substrate. The growth of characteristics of tomato seedlings were promoted by adding 1 to 3g/kg of bioceramics, 10 to 80g/kg of crushed shell or 20 to 40g/kg of elvanites, respectively. Especially, root growth was greatly influenced by bioceramic powder, whereas the shoot growth(leaves and stem) was stimulated by crushed shells and elvanites suppemented into substrate.

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Hair growth promoting effects of emodin in telogenic C57BL/6 mice

  • Yon, Jung-Min;Park, Seul Gi;Lin, Chunmei;Gwon, Lee Wha;Lee, Jong-Geol;Baek, In-Jeoung;Lee, Beom Jun;Yun, Young Won;Nam, Sang-Yoon
    • Korean Journal of Veterinary Research
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    • v.56 no.2
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    • pp.97-101
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    • 2016
  • Emodin is an anthraquinone derivative from the roots of Rheum officinale Baill that possesses a variety of biological activities, including inhibition of $5{\alpha}$-reductase and prostaglandin D2. In this study, we investigated whether emodin promotes hair growth. After emodin was topically applied to the shaved dorsal skin of telogenic C57BL/6 N mice, the hair growth rate and morphological analysis were evaluated in dorsal skin for 15 days. After 13 days of treatment, minoxidil or emodin (0.01% or 0.1%)-treated groups showed remarkable regrowth of hairs relative to the vehicle control group. Scoring of the hair growth and rate of hair growth area for 15 days revealed that groups treated with minoxidil and 0.1% emodin were significantly higher than the vehicle control group. Histological examination revealed the emodin and minoxidil groups markedly recovered the number and morphology of hair follicles, including the subcutis depth, relative to the vehicle group. These results suggest that emodin has an excellent promoting effect in hair growth similar to that of minoxidil and might be useful for treatment of baldness or alopecia.

Changes of Digestive Enzyme Activity in Slime Flounder, Micromus achne Larvae with Growth and Development (찰가자미, Micromus achne 자어의 성장과 발달에 따른 소화효소 활성의 변화)

  • 박상언;임한규;한현섭;이종하;임영수;이종관;이상민
    • Journal of Aquaculture
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    • v.16 no.4
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    • pp.233-239
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    • 2003
  • Growth and activities of digestive enzymes in slime flounder (Microstomus achne) larvae were measured from hatching to near the end of larval development (day 58). Larvae reared under starved and fed conditions and the changes of acid phosphatase (ACPase) specific activity, alkaline phosphatase (ALPase) specific activity, trypsin-like enzyme activity and pepsin-like enzyme activity were described with growth and developmental stage of larvae. Total length of the starved larvae was gradually increased for 7 days post hatching and then almost unchanged. Total length of the fed larvae ranged from 5.13$\pm$0.178 mm at the day of hatching to 13.43$\pm$1.395 mm at 58 days after hatching. In starved group, dry body weight decreased from 0.l0$\pm$0.020 mg at the day of hatching to 0.05$\pm$0.012 mg at 12 days after hatching. Dry body weight of fed larvae decreased during the prelarva stage like starved group and then gradually increased. ACPase and ALPase specific activity in the starved larvae increased until all larvae died, however those activities in the fed larvae increased until 20 days and then decreased until 58 days after hatching, with no significant difference between groups. Trypsin-like enzyme activity in the starved larvae was unchanged until 3 days and then was the highest on 5 days after hatching, but not detected after completion of yolksac absorption. Those of fed larvae decreased until 3 days and sharply increased until completion of yolksac absorption. The highest trypsin-like enzyme activity in the fed group was observed at 20 days after hatching. Trypsin-like enzyme activity in the fed larvae was significantly higher than that in the starved larvae from 8 days after hatching. Pepsin-like enzyme activity was increased in 5 days after hatching in both groups. There was significant difference at 8 and 10 days after hatching between both groups. Based on above results, digestive enzyme activities were correspondingly changed to a growth and morphological transformation. Trypsin-like enzyme and pepsin-like enzyme activities are able to be a useful indices for health and growth status in larval slime flounder, because there was significant difference in digestive enzyme activities with developmental stages, growth or feed supply.

Directional Asymmetry of Gonadal Development in Ayu (Plecoglossus altivelis)

  • Park In-Seok;Zhang Chang Ik;Kim Young Ja;Bang In Chul
    • Fisheries and Aquatic Sciences
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    • v.8 no.4
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    • pp.207-212
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    • 2005
  • Directional (sinistral) asymmetry (DA) occurs when the traits of one side of a supposedly bilaterally symmetrical organism differ in a random way from those of the other side. We examined asymmetries in gonadal growth traits within both sexes of hatchery reared ayu, Plecoglossus altivelis. The pattern of the gonadal growth from hatching to 320 days after hatching was a type of DA except for the growth in gonad weight from 140 to 180 days after hatching, although the ovary of the right side tended to exhibit more pronounced DA phenomenon.

Growth Rate study of CPAE Cells and Osteobalst by Local Hyperthermia Duplex Stainless Steel Thermo-rod (국소온열치료용 듀플렉스 스테인리스 스틸 발열체에 의한 혈관세포와 골세포의 온도에 따른 성장률 변화 관찰)

  • Choi, Sung-Min;Kim, Young-Kun
    • The Journal of the Korea Contents Association
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    • v.9 no.11
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    • pp.247-253
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    • 2009
  • We investigated the cell growth rate according to the change of temperature of the Thermo-rod used for the local hyperthermia therapy. For this study, we fabricated the Thermo-rods (TR) using Duplex Stainless Steels having magnetic properties as well as non magnetic properties. To evaluate cell growth rates up to 15 days, we conducted cell proliferation test using cell counting methods. For the tests, the CAPEs and Osteoblats were seeded on the 6-we11 plates with the induction heated thermo-rods 30 mins a day for 15 days with 2 days interval and without induction heated thermo-rods as control group respectively. We calculated cell growth rates, 6 hours after heating. From the results, in case of CAPEs and Osteobalsts seeded groups, the cell growth rates in all groups increased drastically for 6 days after seeding, but decreased irregularly after 6 days. In conclusion, the cell growth rates showed no significant difference among all groups and it indicated that there were no effects of temperate ($41^{\circ}C$) on cell growth rates.

Effect of Intermittent Feeding on Growth, Feed Utilization and Body Composition of Subadult Olive Flounder Paralichthys olivaceus in Suboptimal Temperature

  • Cho, S.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.3
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    • pp.394-399
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    • 2011
  • The effect of intermittent feeding on growth, feed utilization and body composition of subadult olive flounder fed extruded pellet (EP) in suboptimal temperature ($13.5{\pm}2.10^{\circ}C$) was determined. Two hundred twenty five subadult fish averaging 272 g were randomly distributed into fifteen of 300 L circular flow-through tanks (fifteen fish per tank). Five treatments with different days of feeding a week were prepared in triplicate: fish were hand-fed with EP to apparent satiation once a day, seven days a week (7DF), which was used as the control group or consecutive six, five, four and three days a week, for 12 weeks, referred to as 6DF, 5DF, 4DF and 3DF treatments, respectively. Weight gains and specific growth rates of fish in 6DF treatment were higher than those of fish in 7DF, 4DF and 3DF treatments, but not different from those of fish in 5DF treatment. Daily feed intake of fish in 6DF and 7DF treatments was higher than that of fish in 5DF, 4DF and 3DF treatments. Feed efficiency and protein efficiency ratio of fish were not different among treatments. There was no difference in the chemical composition of fish between treatments. Results of this study demonstrated that feeding five days per week could be recommended for subadult olive flounder fed extruded pellet in suboptimal temperature.

Effect of Phos phatic Fertilization Level on Growth , Crude Potein Content and Nitrohen Fixation Ability of Alfalfa ( Medicago sativa L. ) at Sowing Year (인산시비수준이 알팔파 ( Medicago sativa L. ) 의 파종초년도 생육 , 조단백질함량 및 질소고정능에 미치는 영향)

  • 김창호;이효원
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.16 no.2
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    • pp.113-120
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    • 1996
  • The experiment was carried out to investigate the effects of phosphate fertilizer level on growth, crude protein content and nitrogen fixation ability of alfalfa(Medicugo sariva L.) at sowing year. The experimental field was conducted using a randomized block design with three replications according to the phosphate application level (200 and 400kfia) in farm of Kongju National University. Research samples were collected at intervals of 7 days from October 7 to December 9 and nitrogen fixation activity of nodule was measured by using the acetylene reduction method (Hardy et al. 1968). The results obtained are summarized as follows : 1. Number of branches, root length and dry matter weight at phosphate fertilizer level of 400kg in sowing year were more increase than those of 200kfia. The number of nodules was not significantly difference between phosphate fertilizer level, because of low temperature to be enough to nodulation of alfalfa. 2. The crude protein content of leaf, stem and root were not significantly affected by the phosphate fertilizer level. but that of leaf was increased at 400kgha on 72 days after sowing. The crude protein content of each part according to growing stages was increased at 44 days and 72 days after sowing. 3. The dry weight of root was not significantly difference between phosphate fertilizer level from 30 days to 51 days after sowing, but that was increased at phosphate fertilizer level of 400kgha from 51 days afier sowing. 4. The amount of ethylene in the samples taken 2 hours and calculated amount of nitrogen fixation were increased at phosphate fertilizer level of 400kfia from 30 days to 65 days after sowing. but there was vice versa from 58 days after sowing.

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Influence of Cu[II] on the Growth of Korean Axolotl, Hynobius leechii (동이온이 도롱뇽유생의 성장에 미치는 영향)

  • Park, Jin Ho;Won Hark Park;Sang Ock Park
    • The Korean Journal of Ecology
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    • v.6 no.2
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    • pp.106-113
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    • 1983
  • The growth of Korean axolotl., Hynobius leechii, was analyzed in natural water as control group and in six copper ion groups contaminated by 0.1, 0.2, 0.3, 0.4, 0.5, 0.6 ppm of copper ion. The copper ion checks the growth of the axolotl. The axolotl was not survived during 30 days in the copper ion of 0.3ppm, and, in the 0.4ppm the axolotl was not survived during 10 days after hatch. The growth of head width and body length show a convexing increase pattern, while that of hind leg shows a concaving increase pattern. The copper ion checks the development of hind leg. In the growth quantity of head width, body length and hid leg, that of natural water show the most rapid increase pattern, and copper ion groups of 0.1, 0.2, 0.3ppm follow in that order. The coefficient of relative growth($\alpha$) of control group is the greater value, and the copper ion groups of 0.1, 0.2, 0.3ppm follow in that order. The contaminated groups show the negative allometry in the relative growth of the containated groups to the natural water. Body length shows positive allometry, while hind leg shows negative allometry in the relative growth to head width.

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Growth Differences in Edible Caulerpa okamurae (Weber-van Bosse) Thallus Parts with and without Apex Removal (식용종 옥덩굴(Caulerpa okamurae Weber-van Bosse)의 부위별 생장과 정단 제거 유·무가 생장에 미치는 영향)

  • Shin, Ji Ha;Park, Seo Kyung;Choi, Han Gil
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.54 no.3
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    • pp.311-317
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    • 2021
  • Growth differences in Caulerpa okamurae thallus parts and the effects of apex removal on growth were examined. Erect fronds, stolons, and erect fronds with stolons (erect+stolon) were grown for 14 days, whereas erect fronds without and with apex removal (-A and +A fronds, respectively), were grown for 25 days. The relative growth rates (RGR) of C. okamurae thallus parts ranged from 1.91% to 4.93% day-1 with maximal and minimal RGR for the stolon and erect+stolon treatments, respectively. Over the 25 days in culture, the -A fronds showed a higher RGR (2.48% day-1) than the +A fronds (1.96% day-1). More new erect fronds and stolons were produced by -A fronds than those by +A fronds. In addition, basal cutting of erect C. okamurae fronds resulted in a greater plant length increase than that obtained from top cutting. These findings indicate that vegetative growth is common in C. okamurae, which has excellent wound healing ability, and cutting damage to erect fronds may stimulate enhanced vegetative growth. Thus, we suggest that stolons and erect fronds of C. okamurae could be used as seeding materials for large scale cultivation. Furthermore, this edible species could be a potential seaweed resource for rapid skin regeneration cosmetics.

Continuous Production of Natural Colorant, Betacyanin, by Beta vulgaris L. Hairy Root

  • Kim, Sun-Hee;Ahn, Sang-Wook;Bai, Dong-Kyu;Kim, Kwang-Soo;Hwang, Baik;Lee, Hyeon-Yong
    • Journal of Microbiology and Biotechnology
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    • v.9 no.6
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    • pp.716-721
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    • 1999
  • It has been known that continuous cultivation of hairy root is difficult to maintain for a long period of time compared to the microbial and callus cultures. Chemostat cultivation was successfully carried out in order to economically produce a plant-based colorant, betacyanin, from red beet hairy root for more than 85 days in a 14-1 fermentor. The result from the chemostat cultivation was compared to those of the batch and fed-batch cultivations of red beet hairy roots. It was shown that hairy root reached its steady state within 50 days of the cultivation, and then maintained for about 25-30 days in a wide range of dilution rates. Total betacyanin production from the continuous process was also calculated to be 2.65g at 0.28(l/d) of dilution rate, compared to 0.196g from fed-batch cultivation. It was found that betacyanin production was a partially growth related process, yielding 0.376 mg/g-fresh wt. cell and $1.89{\times}10^{-5}$ mg/g-fresh wt. cell/d, with 0.92 of correlation factor in a partial growth-product model. It was also shown that the cell growth required was relatively large for maintenance amount of energy at a low dilution rate. The growth of hairy root was inhibited by high light intensity in following a photo-inhibition model. The growth parameters were estimated to be 0.3(l/d), $10.56kcal/\textrm{m}^2/h$,{\;}and{\;}35.81kcal/\textrm{m}^2/h$ for the maximum specific growth rate, half saturation light intensity, and inhibition light intensity, respectively.

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