• 산업경영시스템학회지
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• 제44권2호
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• pp.66-77
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• 2021

Companies widely use survey to identify customer requirements, but the survey has some problems. First of all, the response is passive due to pre-designed questionnaire by companies which are the surveyor. Second, the surveyor needs to have good preliminary knowledge to improve the quality of the survey. On the other hand, text mining is an excellent way to compensate for the limitations of surveys. Recently, the importance of online review is steadily grown, and the enormous amount of text data has increased as Internet usage higher. Also, a technique to extract high-quality information from text data called Text Mining is improving. However, previous studies tend to focus on improving the accuracy of individual analytics techniques. This study proposes the methodology by combining several text mining techniques and has mainly three contributions. Firstly, able to extract information from text data without a preliminary design of the surveyor. Secondly, no need for prior knowledge to extract information. Lastly, this method provides quantitative sentiment score that can be used in decision-making.

• 정보처리학회논문지D
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• 제15D권1호
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• pp.23-30
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• 2008

유비쿼터스 환경에서 상황정보 인식 분야를 연구하면서 가장 밑바탕에서 기초가 될 수 있는 것은 인터넷 기술과 XML(Extensible Markup Language)이다. 인터넷을 통한 통신에서 XML 데이터의 사용이 일반화되고 있으며 데이터의 형태는 연속적이다. 그리고 XML 스트림 데이터에 대한 질의를 처리하기 위한 방안들이 제시되고 있다. 이 논문에서는 스트림 데이터에 대한 질의처리를 효율적으로 수행하기 위한 기반연구로써 XML을 레이블의 순서화된 트리로 모델링하여 온라인 환경에서 빈발한 구조를 추출하는 마이닝 방법을 제안한다. 즉, 지속적으로 입력되는 XML 데이터의 구조를 트리로 모델링하고 각각의 트리를 하나의 트리 집합의 구조로 표현하여 현재 윈도우 시점에서 빈발한 구조를 정확하고 빠르게 추출하는 방법을 제시한다. 제시하는 방법은 XML의 질의 처리 및 색인 구성의 기초 자료로 활용될 수 있다.

• 한국자원식물학회지
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• 제25권5호
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• pp.543-549
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• 2012

대청의 이용성 향상을 위한 자료 확보 측면에서 식물체 부위 및 추출 용매별에 따른 항산화 효소 활성 및 항균효과를 조사하였다. APX(Ascorbate Peroxidase) 활성은 줄기의 에탄올 추출물, 잎의 메탄올 추출물, 잎의 증류수의 추출물 순으로 높아 각각 1601.7, 1133.7 및 524.3(Unit/mg protein)을 나타냈다. CAT(Catalase) 활성은 꽃의 에탄올 추출물, 잎의 메탄올 추출물, 꽃의 증류수 추출물 순으로 높았는데, 각각 177.1, 120.8 및 55.4(Unit/mg protein)를 나타냈다. POD(Ascorbate Peroxidase) 활성은 꽃의 에탄올 추출물, 꽃의 메탄올 추출물, 줄기의 증류수 추출물 순으로 높았으며, 각각 27.1, 14.6 및 10.4(Unit/mg protein)를 나타냈다. SOD(superoxide dismutase) 활성은 뿌리의 증류수 추출물, 꽃의 메탄올 추출물, 뿌리의 에탄올 추출물 순으로 높았으며, 각각 90.8, 80.1, 75.5%를 나타냈다. 대청의 꽃 추출물은 Vibrio parahaemolyticus에 대해서만, 뿌리는 Staphy lococcus aureus에 대해서만, 줄기 추출물은 Bacillus subtilis, Escherichia coli, Staphy lococcus aureus에 대해서만 용매에 관계없이 항균활성을 나타냈다. 특히 대청 잎의 증류수 추출물은 Bacillus subtilis, Escherichia coli에 대해 높은 항균활성을 나타내어 저해환 직경이 각각 30.0 및 24.0 mm이었다. 이와 같은 결과는 약용식물로서 대청의 가치가 높음을 시사해 주었다.

• 대한본초학회지
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• 제22권2호
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• pp.73-78
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• 2007

Objectives : Uncontrolled activation of microglia may directly toxic to neurons by releasing various substances such as inflammatory cytokines, nitric oxide(NO), prostaglandin E2 and superoxide. In this study, the effects of the several subfractions isolated from Coptidis Rhizoma extract were investigated on NO production in LPS-stimulated BV2 microglial cells, Methods : Coptidis Rhizoma extract prepared with 80% methanol, and then fractionated with ethylacetate, chloroform, n-butanol and water. BV2 cells were pretreated four subfractions of Coptidis Rhizoma with various concentrations, and then stimulated with LPS. Cytotoxicity of each fraction was measured by MTT assay. NO production was determined in culture surpernatants by Griess reagent. Results : Ethylacetate, chloroform and butanol fractions of Coptidis Rhizoma extract significantly decreased LPS-induced NO production in BV2 cells as a dose-dependent manner without cytotoxicity. Ethylacetate fraction of Coptidis Rhizoma extract was most effective on inhibition of NO production in LPS-stimulated BV2 cells compared with other fractions. Conclusion : This data indicates that Ethylacetate fraction of Coptidis Rhizoma extract shows strong antiinflammatory effects through inhibition of LPS-induced microglial activation.

• 대한본초학회지
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• 제21권2호
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• pp.165-173
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• 2006

Objectives : Coptidis Rhizoma has been known traditional medicine with antimicrobial activities. We investigated inhibitory effects of Coptidis Rhizoma extract on lipopolysaccharide(LPS)-induced nitric oxide production from mouse macrophages. Methods : After Coptidis Rhizoma extract was pretreated in BV2, mouse brain macrophages and RAW264.7 mouse macrophages, cells were activated with LPS. To investigate cytotoxicity Coptidis Rhizoma extract, cell viability was measured by MTT assay. The production of nitric oxide(NO) and inducible nitric oxide synthase(iNOS) was determined in each culture supernatant and mRNA by Griess reaction and RT-PCR. The production of $TNF-{\alpha}$ from cells was measured by ELISA. Results : Coptidis Rhizoma extract significantly inhibited LPS-induced NO production in BV2 and RAW264.7 cells. Coptidis Rhizoma extract also greatly suppressed mRNA expression of iNOS in BV2 and RAW264.7 cells activated by LPS. Conclusion : These data suggests that Coptidis Rhizoma extract may have an anti-inflammatory effect through the inhibition of NO production.

• Asian Pacific Journal of Cancer Prevention
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• 제16권2호
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• pp.565-570
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• 2015

Breast and colorectal cancers rank high in Iran as causes of mortality. Most of the current treatments are expensive and non-specific. The potential anticancer properties of common home gecko, Cyrtopodion scabrum, were investigated in this study. The effects of C. scabrum extract on proliferation, viability and migration of the colorectal cancer (SW-742), breast cancer (MCF-7) and normal (MSC) cell lines were investigated using MTT and in vitro wound healing assay. $IC_{50}$ values calculated for the extract were $559{\pm}28.9{\mu}g/mL$ for MCF-7 and $339{\pm}11.3{\mu}g/mL$ for SW-742. No toxic effects on the normal control cells were observed. MCF-7 and SW-742 cell growth was inhibited by 32.6% and 62%, under optimum conditions, compared to the untreated control cells. The extract also decreased the motility and migration ability of both cancer cell lines, with no significant effects on the normal control cells. Data suggest C. scabrum extract as a useful natural resource for targeting cancer cells specifically.

• 한국수산과학회지
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• 제49권2호
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• pp.124-130
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• 2016

To effectively remove salts from Suaeda japonica, extracts, an electrodialysis system was developed. The biological activities of non-desalted (NDS) and desalted S. japonica (DS) extracts were compared. The DS extract exhibited superior polyphenolic (6.26%) and carbohydrate (28.56%) contents. The IC₅₀ values of the DS extract against DPPH radicals and hydrogen peroxide were 0.22 and 0.68 mg/mL, respectively, which was higher than that of the NDS extract. Neither the DS nor the NDS extract was cytotoxic in RAW 264.7 macrophages. Additionally, the DS extract had a higher NO inhibitory effect compared to the NDS extract in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. These data indicate that DS extracts have greater biological activity than do ND extracts, and application of the electrodialysis process may be useful in marine bioresource applications.

• 한국치위생학회지
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• 제14권1호
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• pp.109-115
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• 2014

Objectives : Prunus mume has been used for the folk medicine from old times. The purpose of the study was to investigate the antimicrobial activity of prunus mume extract to various oral microbes. Methods : This study was carried out to examine the antimicrobial effect of Prunus mume extract against oral microbes. Data were collected using a Dentocult SM Strip mutans and Dentocult LB Strip mutans from April 5 to May 4, 2013. A total of 36 experimental and 32 control group were selected for this study. Results : The MIC of Prunus mume extract was tested for 0.39% in S. mutans, S. salivarius and S. auerus, 0.78% in S. mitis, S. equi and E. coli. In vivo, experimental group showed significantly the lower Streptococcus mutans levels by the use of the gum contained Prunus mume extract from 15 days compared with control group(p=0.012). The reduction was more significant in Lactobacilli level of the experimental group than the control group(p=0.022). Conclusions : These findings suggest that the oral products containing Prunus mume extracts is effective in preventing oral diseases.

• Advances in Traditional Medicine
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• 제8권1호
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• pp.24-31
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• 2008

In the present investigation, we studied the effect of alcoholic extract of Moringa oleifera (M. oleifera) seed kernels on various experimental models of bronchial asthma. Significant (P < 0. 05) increase in preconvulsion time was observed due to pretreatment with M. oleifera when the guinea pigs were exposed to either acetylcholine (Ach) or histamine aerosol. This bronchodilating effect of M. oleifera was comparable to ketotifen fumarate. Spasmolytic effect of M. oleifera was also observed by dose dependent inhibition of ideal contractions induced by Ach, 5HT, histamine and $BaCl_2$. Alcoholic extract of M. oleifera produced significant dose dependent protection by egg albumin and compound 48/80 induced mast cell degranulation. Pretreatment with alcoholic extract of M. oleifera also decreased carrageenan induced rat paw edema, which was comparable to that of standard diclofenac sodium. Minimum inhibitory concentration for alcoholic extract of M. oleifera was low as compared to cold-water extract and hot water extract when antimicrobial activity was tested against various respiratory pathogens like Escherichia coli (E. coli), Staphylococus aureus (S. aureus) and pseudomonas aeruginosa (P. aeruginosa). Our data suggest that antiasthmatic activity of M. oleifera seed kernels may be due to its bronchodilator, anti-inflammatory, mast cell stabilization and antimicrobial activity.

• 한국자원식물학회지
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• 제28권3호
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• pp.321-328
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• 2015

Alpiniae officinarum Rhizoma (the rhizome of Alpinia officinarum Hance, known as lesser galangal), a family of Zingiberaceae, has been used to reduce pain of infection and inflammatory diseases in Asian countries. The present study was focused to evaluate the inhibitory degranulation effect of Alpiniae officinarum Rhizoma extract in RBL-2H3 rat basophilic leukemia cells. Cell viability was measured by MTT assay. RBL-2H3 cells were stimulated by phorbol 12-myristate 13-acetate and calcium ionophore A23187. Mast cell degranulation was analyzed by measuring release of β-hexosaminidase in RBL-2H3 cell. Gene expression was measured by qRT-PCR and signaling molecules were detected by immunoblotting. The Alpiniae officinarum Rhizoma extract suppressed β-hexosaminidase release in dose-dependent manner and inhibited cycloxygenase-2 and tumor necrosis factor-α gene expression. Furthermore, it was found that Alpiniae officinarum Rhizoma extract reduced mitogen-activated protein kinases, especially phosphorylated p38, at 0.75 ㎎/㎖ of Alpiniae officinarum Rhizoma extract concentrations. These data show that Alpiniae officinarum Rhizoma extract has immunosuppressive effect in mast cell induced allergic inflammation.