• Journal of Yeungnam Medical Science
• /
• v.12 no.1
• /
• pp.124-134
• /
• 1995

It is the most important to select optimal culture conditions to promote safe embryo growth in the technique of human in vitro fertilization and embryo transfer. It has been shown that the addition of biologic fluids, such as blood serum, of various origins, improved fertilization and early cleavage rates in numerous species. The purpose of this study is to attempt to measure developmental potential of mouse eggs fertilized and cleaved in Ham's F10 culture medium containing a chelating agent, EDTA and fetal cord serum. In this study, we selected 40 female mice and 20 male mice, and investigated optimal serum concentrations for mouse embryo growth. Two cell stage mouse embryos were cultured in Ham's F-10 medium, Ham's F-10 medium with various concentrations of EDTA, or Ham's F-10 medium with EDTA and 10% human cord serum. Developmental ratios to morula in Ham's F-10 medium containing various concentrations of EDTA and/or 10% fetal cord serum were significantly higher than in unsupplemented Ham's F-10 medium (p<0.05). Developmental ratios to blastocyst in Ham's F-10 containing 10% fetal cord serum and $50{\mu}M$ or $100{\mu}M$ EDTA were significanltly higher than in unsupplemented Ham's F-10 medium (p<0.05). Developmental ratios to morula in Ham's F-10 containing 10% fetal cord serum and $100{\mu}M$ EDTA were significanltly higher than in Ham's F-10 with 10% fetal cord serum used commonly in many human IVF centers(p<0.05). Developmental ratio to blastocyst in Ham's F-10 containing 10% fetal cord serum and $100{\mu}M$ EDTA was significanlty higher than in Ham's F-10 with $200{\mu}M$ EDTA(P<0.05). In summary, embryo development to morula and blastocyst was significanlty higher in the presence of human cord serum or EDTA than in the unsupplemented medium. The most significanly development to morula and blastocyst was obtained at Ham's F-10 medium with $100{\mu}M$ concentration of EDTA and 10% fetal cord serum. These results suggest that Ham's F-10 medium containing 10% fetal cord serum and optimal concentrations of EDTA significantly promoted early cleavage of mouse zygotes, and these will be useful as basic data for the selection of culture medium in human in vitro fertilization.

• Journal of agriculture & life science
• /
• v.43 no.1
• /
• pp.51-59
• /
• 2009

The chemical components and antimicrobial activities of garlic from different area were investigated and analyzed to provide basic data for functional food materialization and processing. Hunter's values of garlic from different area were L 53.41~57.15, a -3.49~-4.38 and b 11.47~17.55. The moisture, crude protein, crude fat, nitrogen free extract, crude fiber and ash were 65.24~71.96, 6.24~9.35, 0.21~0.49, 19.01~22.72, 0.58~0.95 and 1.01~2.01%, respectively. The major minerals of garlic from different area were Na(27.22~112.03), Mg(18.17~32.56), K(242.16~569.28), Ca(28.60~63.93), P(117.72~265.21 mg%) and major free sugars were sucrose, glucose and fructose. The major amino acids of garlic from different area were proline, arglmne, glutamic acid and aspartic acid and content of total amino acid was 2,709.33~4,561.04 mg%. The ascorbic acid content of garlic from different area was 2.966~8.673 mg%. Composition of fatty acids of garlic from different area were linoleic acid, oleic acid and palmitic acid, unsaturated fatty acid and saturated fatty acid contents were 72.18~74.35 and 25.65~27.82%, respectively. Antimicrobial activities of garlic extracts as different area increased depends on concentration and showed the high antimicrobial activities against Gram(+) and Gram(-).

• Korean Journal of Soil Science and Fertilizer
• /
• v.4 no.1
• /
• pp.13-19
• /
• 1971

In order to establish the method of improving ill drained paddy soil where the accumulation of absorption inhibitor is worried in the earlier stages of rice growth, proper soil is selected and an field experiment is designed having treatments such as lime materials, none sulfate fertilizers, boron and straw etc. The data of yield and plant analysis in different stages of rice growth is eveluated and discussed to obtain following summaries. (1) Significant yield increase was made by the treatment of lime materials such as slacked lime or wollastonite powder, materials inhibiting the activity of microorganisms such as boron and of none sulfate fertilizers lacking inhibitor producing sources. (2) The crop scientifice causes of decreasing yield are the decreasing the number of panicles per hill, grains per panicle and the weight of grains. (3) The plant nutritional causes of decreasing yield are the lowering of nitrogen content throughout the life, phosphate content since young premodia formation stage of plant and the decreased content of magnesium, calcium and silicate in straw at harvesting stage. (4) The causes of lowering the content of various elements in rice plant grown in ill drained paddy soil are suggested as root damage by producing and accumulating absorption inhibitors such as organic acids and hydrogen sulfide etc, from the following observed facts; (a) In young premodia formation stage, attaining to the maximum production and accumulation of absorption inhibitor, the phosphate accumulation in plant was smaller in the phosphate plots than without phosphate plots and much higher in the neutralized plots by adding lime materials. (b) In the plots of straw addition, the potassium content in plant at the young premodia formation stage is very low probabley due to root damage by absorption inhibitor produced from the process of straw decomposition but higher at the stage of harvesting probably due to the immetabolic negative absorption of damaged roots. (c) The effect of boron, known as the inhibitor of microorganism activity to decompose organic matter, is apparent. (d) The effect of nonsulfate fertilizer treatment, having no source of producing inhibitor such as hydrogen sulfide, was significant. (e) All the yield components, decided around the young premodia formation stage attaining to the maximum inhibitor concentration in soil and minimum root activity, are significantly decreased.

• Korean Journal of Psychosomatic Medicine
• /
• v.24 no.2
• /
• pp.227-235
• /
• 2016

Objectives : The aims of this study were to know the frequency of cognitive dysfunction among patients with autoimmune thyroid disorders, and to reveal influencing factors on it, especially to clarify association with autoimmune thyroid antibodies. Methods : From sixty-five female patients with autoimmune thyroid disorders, demographic data were obtained by structured interview. Their cognitive funtions were measured using the MMSE-K and the MoCA-K tests. Depression was evaluated by the K-HDRS. Results : 1) Among patients with autoimmune thyroid disorders, 7.69% of them were below 24 on the MMSE-K, while 10.77% were below 22 on the MoCA-K. The frequency of cognitive deficit was not significantly different according to having positivity to antimicrosomal antibodies or not. 2) The antimicrosomal antibody-positive patients had significantly higher antithyroglobulin antibody titers, antimicrosomal antibody titers, and TSH concentration, while had significantly lower free T4 levels(p<0.05, respectively). 3) The total scores of the MMSE-K and the MoCA-K had significant correlation with age, marital status, antithyroglobulin antibody titers and K-HDRS(p<0.05, respectively). 4) The regression analysis revealed that variables such as age, education, autoimmune thyroid antibodies, thyroid function and depression did not influence on cognitive function of patients with autoimmune thyroid disorders. Conclusions : Our results could not support that cognitive function of patients with autoimmune thyroid disorders had correlation with autoimmune thyroid antibodies.

• Korean Journal of Remote Sensing
• /
• v.37 no.6_1
• /
• pp.1559-1572
• /
• 2021

The response of vegetation under the crop stress condition was evaluated using structural, biochemical, and physiological vegetation indices based on unmanned aerial vehicle (UAV) images and field-spectrometer data. A high concentration of herbicide was sprayed at the different growth stages of garlic to process crop stress, the above ground dry matter of garlic at experimental area (EA) decreased about 46.2~84.5% compared to that at control area. The structural vegetation indices clearly responded to these crop damages. Spectral reflectance at near-infrared wavelength consistently decreased at EA. Most biochemical vegetation indices reflected the crop stress conditions, but the meaning of physiological vegetation indices is not clear due to the effect of vinyl mulching. The difference of the decreasing ratio of vegetation indices after the herbicide spray was 2.3% averagely in the case of structural vegetation indices and 1.3~4.1% in the case of normalization-based vegetation indices. These results meant that appropriate vegetation indices should be utilized depending on the types of crop stress and the cultivation environment and the normalization-based vegetation indices measured from the different spatial scale has the minimized difference.

• The Journal of Society for e-Business Studies
• /
• v.26 no.3
• /
• pp.33-53
• /
• 2021

In the crossroads of social change caused by the spread of the Fourth Industrial Revolution and the prolonged COVID-19, the Korean government announced the Digital New Deal policy on July 14, 2020. The Digital New Deal policy's primary goal is to create new businesses by accelerating digital transformation in the public sector and industries around data, networks, and artificial intelligence technologies. However, in a rapidly changing social environment, information asymmetry of the future benefits of technology can cause differences in the public's ability to analyze the direction and effectiveness of policies, resulting in uncertainty about the practical effects of policies. On the other hand, the media leads the formation of discourse through communicators' role to disseminate government policies to the public and provides knowledge about specific issues through the news. In other words, as the media coverage of a particular policy increases, the issue concentration increases, which also affects public decision-making. Therefore, the purpose of this study is to verify the dynamic relationship between the media coverage and the stock market on the Korean government's digital New Deal policy using Granger causality, impulse response functions, and variance decomposition analysis. To this end, the daily stock turnover ratio, daily price-earnings ratio, and EWMA volatility of digital technology-based companies related to the digital new deal policy among KOSDAQ listed companies were set as variables. As a result, keyword search volume, daily stock turnover ratio, EWMA volatility have a bi-directional Granger causal relationship with media coverage. And an increase in media coverage has a high impact on keyword search volume on digital new deal policies. Also, the impulse response analysis on media coverage showed a sharp drop in EWMA volatility. The influence gradually increased over time and played a role in mitigating stock market volatility. Based on this study's findings, the amount of media coverage of digital new deals policy has a significant dynamic relationship with the stock market.

• Journal of the Korean earth science society
• /
• v.42 no.3
• /
• pp.278-295
• /
• 2021

China's shutdown due to COVID-19 in 2020 reduced air pollutant emissions, which is located on the windward side of South Korea. The positive temperature anomaly and negative zonal wind anomaly from northern Mongolia to South Korea through eastern China presented warm and stationary air masses during January and February 2020. Decreased concentrations of PM10, NO₂, and O₃ were measured at Seokmo-ri and Pado-ri, located in the central-western region of South Korea, due to decreased emissions in China from January to March 2020. After China's shutdown from January to March 2020, in Pado-ri, the ratio of monthly average concentrations in that period with those of PM10 and O₃ in the last four years decreased by approximately 0.7-4.7% and 9.2-22.8%, respectively. In January 2020, during the Lunar New Year holidays in China, concentrations of PM10, NO₂, and O₃ at Seokmo-ri and Pado-ri decreased just as much as it did during the same period in the last four years. However, average concentrations in January 2020 decreased before and after the Lunar New Year holidays in China when compared with those in January of the last four years. In Seokmori, ratios of actual and predicted values (${\bar{O}_s$/M) for PM10, NO₂, and O₃ concentrations were calculated as 70.8 to 89.7%, 70.5 to 87.1%, and 72.5 to 97.1%, respectively, during January and March 2020. Moreover, those of Pado-ri were 79.6 to 93.5%, 67.7 to 84.9%, and 83.7 to 94.6%, respectively. In January 2020, the aerosol optical depth (AOD) data showed a higher distribution than that of the last four years due to photochemical reactions in regions from northern Mongolia to eastern China and the Korean Peninsula. However, the decrease in AOD values compared with those of the last four years was attributed to the decrease in emissions of precursors that generate secondary aerosols in China during March 2020.

• Pediatric Infection and Vaccine
• /
• v.26 no.1
• /
• pp.1-10
• /
• 2019

Purpose: We investigated the distribution and antimicrobial resistance of pneumococcal isolates from hospitalized children at Asan Medical Center for recent 4 years, and aimed to recommend proper choice of empirical antibiotics for pneumococcal infection. Methods: From March 2014 to May 2018, children admitted to Asan Medical Center Childrens' Hospital with pneumococcal infection were subjected for evaluation of minimal inhibitory concentration (MIC) for ${\beta}-lactams$ and macrolide antibiotics. Patient's age, underlying disease, gender were retrospectively collected. Using Monte Carlo simulation model and MIC from our study, we predicted the rate of treatment success with amoxicillin treatment. Results: Sixty-three isolates were analyzed including 20.6% (n=13) of invasive isolates, and 79.4% (n=50) of non-invasive isolates; median age were 3.3 years old, and 87.3% of the pneumococcal infections occurred to children with underlying disease. Overall susceptibility rate was 49.2%, 68.2%, and 74.6% for amoxicillin, parenteral penicillin, and cefotaxime respectively. 23.8% and 9.5% of the isolates showed high resistance for amoxicillin, and cefotaxime. Only 4.8% (n=3) were susceptible to erythromycin. Monte Carlo simulation model revealed the likelihood of treatment success was 46.0% at the dosage of 90 mg/kg/day of amoxicillin. Conclusions: Recent pneumococcal isolates from pediatric patients with underlying disease revealed high resistance for amoxicillin and cefotaxime, and high resistance for erythromycin. Prudent choice of antibiotics based on the local data of resistance cannot be emphasized enough, especially in high risk patients with underlying disease, and timely vaccination should be implemented for prevention of the spread of resistant strains.

• Journal of Food Hygiene and Safety
• /
• v.34 no.1
• /
• pp.13-21
• /
• 2019

A method was developed for the simultaneous detection of an antibiotic fungicide, streptomycin, and its metabolite (dihydrostreptomycin) in agricultural products using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The samples were extracted using methanol adjusted to pH 3 using formic acid, and purified with a HLB (Hydrophilic lipophilic balance) cartridge. The matrix-matched calibration curves were constructed using seven concentration levels, from 0.001 to 0.1 mg/kg, and linearity of five agricultural products (hulled rice, potato, soybean, mandarin, green pepper), with coefficients of determination $(R^2){\geq}0.9906$, for streptomycin and dihydrostreptomycin. The mean recoveries at three fortification levels (LOQ, $LOQ{\times}10$, $LOQ{\times}50$, n = 5) were from 72.0~116.5% and from 72.1~116.0%, and relative standard deviations were less than 12.3% and 12.5%, respectively. The limits of quantification (LOQ) were 0.01 mg/kg, which are satisfactory for quantification levels corresponding with the Positive List System. All optimized results satisfied the criteria ranges requested in the Codex guidelines and the Food Safety Evaluation Department guidelines. The present study could serve as a reference for the establishment of maximum residue limits and be used as basic data for detection of streptomycin and dihydrostreptomycin in food.

• Journal of the Korean earth science society
• /
• v.40 no.3
• /
• pp.227-239
• /
• 2019

Heavy snowfall events frequently occur in the Gangwon province, and the snowfall amount significantly varies in space due to the complex terrain and topographical modulation of precipitation. Understanding the spatial characteristics of heavy snowfall and its prediction is particularly challenging during snowfall events in the easterly winds. The easterly wind produces a significantly different atmospheric condition. Hence, it brings different precipitation characteristics. In this study, we have investigated the microphysical characteristics of snowfall in the windward and leeward sides of the Taebaek mountain range in the easterly condition. The two snowfall events are selected in the easterly, and the snow particles size distributions (SSD) are observed in the four sites (two windward and two leeward sites) by the PARSIVEL distrometers. We compared the characteristic parameters of SSDs that come from leeward sites to that of windward sites. The results show that SSDs of windward sites have a relatively wide distribution with many small snow particles compared to those of leeward sites. This characteristic is clearly shown by the larger characteristic number concentration and characteristic diameter in the windward sites. Snowfall rate and ice water content of windward also are larger than those of leeward sites. The results indicate that a new generation of snowfall particles is dominant in the windward sites which is likely due to the orographic lifting. In addition, the windward sites show heavy aggregation particles by nearby zero ground temperature that is likely driven by the wet and warm condition near the ocean.