• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.187-190
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• 2000

Effects of gamma ionizing radiation on recombinant Escherichia coli cells containing stress promoters, recA, fabA, grpE, or katG, fused to luxCDABE originated from Vibrio fischeri were characterized by monitoring transcriptional responses reflected by bioluminescent output. Quantification of gamma-ray intensity may be possible using the recA and fabA promoter fusion since a linear enhancement of bioluminescence emission with increasing gamma-ray intensity was observed. Other strains sensitive to either oxidative stress (DPD2511, katG::luxCDABE) or protein-damaging stress (TV1061, grpE::luxCDABE) were also irradiated by gamma-rays, and resulted in no noticeable bioluminescent output while DPD2794 with recA promoter and DPD2540 with fabA promoter irradiated by the same intensities of gamma-rays gave a significant bioluminescent output. This indicates that the main stresses in the recombinant bacteria caused by ionizing radiation are DNA and membrane-damage, not protein- or oxidative-damage. In addition, in this study, to investigate the relationship between the radiation dose rate and bacterial responses, two recombinant Escherichia coli strains, DPD2794 and GC2, containing lac promoter fused to luxCDABE originating from Photorhapdus luminescences, were used for detecting DNA damage and cellular toxicity under various radiation dose rates. Throughout this study, it was found that these bacteria showed quantitative stress responses to DNA damage and general toxicity caused by gamma rays, depending on the radiation dose rates, indicating that the bacterial stress responses and general toxicity were seriously influenced according to radiation dose rates.

• Smart Structures and Systems
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• v.15 no.1
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• pp.1-13
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• 2015

This paper proposes an analytical mode decomposition (AMD) and Hilbert transform method for structural nonlinearity quantification and damage detection under earthquake loads. The measured structural response is first decomposed into several intrinsic mode functions (IMF) using the proposed AMD method. Each IMF is an amplitude modulated-frequency modulated signal with narrow frequency bandwidth. Then, the instantaneous frequencies of the decomposed IMF can be defined with Hilbert transform. However, for a nonlinear structure, the defined instantaneous frequencies from the decomposed IMF are not equal to the instantaneous frequencies of the structure itself. The theoretical derivation in this paper indicates that the instantaneous frequency of the decomposed measured response includes a slowly-varying part which represents the instantaneous frequency of the structure and rapidly-varying part for a nonlinear structure subjected to earthquake excitations. To eliminate the rapidly-varying part effects, the instantaneous frequency is integrated over time duration. Then the degree of nonlinearity index, which represents the damage severity of structure, is defined based on the integrated instantaneous frequency in this paper. A one-story hysteretic nonlinear structure with various earthquake excitations are simulated as numerical examples and the degree of nonlinearity index is obtained. Finally, the degree of nonlinearity index is estimated from the experimental data of a seven-story building under four earthquake excitations. The index values for the building subjected to a low intensity earthquake excitation, two medium intensity earthquake excitations, and a large intensity earthquake excitation are calculated as 12.8%, 23.0%, 23.2%, and 39.5%, respectively.

• Structural Engineering and Mechanics
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• v.78 no.3
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• pp.351-368
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• 2021

This paper, by applying a reliability-based framework, develops seismic vulnerability macrozonation maps for Tehran, the capital and one of the most earthquake-vulnerable city of Iran. Seismic performance assessment of 3-, 4- and 5-story steel moment resisting frames (SMRFs), designed according to ASCE/SEI 41-17 and Iranian Code of Practice for Seismic Resistant Design of Buildings (2800 Standard), is investigated in terms of overall maximum inter-story drift ratio (MIDR) and unit repair cost ratio which is hereafter known as "damage ratio". To this end, Tehran city is first meshed into a network of 66 points to numerically locate low- to mid-rise SMRFs. Active faults around Tehran are next modeled explicitly. Two different combination of faults, based on available seismological data, are then developed to explore the impact of choosing a proper seismic scenario. In addition, soil effect is exclusively addressed. After building analytical models, reliability methods in combination with structure-specific probabilistic models are applied to predict demand and damage ratio of structures in a cost-effective paradigm. Due to capability of proposed methodology incorporating both aleatory and epistemic uncertainties explicitly, this framework which is centered on the regional demand and damage ratio estimation via structure-specific characteristics can efficiently pave the way for decision makers to find the most vulnerable area in a regional scale. This technical basis can also be adapted to any other structures which the demand and/or damage ratio prediction models are developed.

• Journal of the Korea Institute of Building Construction
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• v.23 no.6
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• pp.797-807
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• 2023

This research aims to investigate the alterations in typhoon patterns attributable to climate change and to quantitatively assess the risk of damage to residential structures. The increasing prevalence of climate anomalies and severe weather events, a consequence of global warming, is causing escalating damage globally. Notably, numerous countries are facing substantial devastation due to shifts in typhoon trajectories. Despite this, there exists a gap in empirical research quantifying the impact of these changes on building integrity and the associated risk alterations driven by climate change. In addressing this gap, our study analyzes the frequency and intensity of typhoons impacting Korea, examining the evolution of these meteorological phenomena. Furthermore, we employ the Korean Typhoon Vulnerability Function for residential facilities to quantify the altered risk posed by these changing patterns. The outcomes of this study provide the private sector with essential data to formulate diverse scenarios and business strategies in response to the escalating risks of typhoon-related damage. Additionally, it equips governmental bodies with the necessary insights to develop comprehensive risk management strategies to mitigate the effects of future typhoons.

• Journal of Korean Society of Forest Science
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• v.97 no.6
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• pp.656-660
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• 2008

This study is carried out to minimize the damage to the forest road when locating strip roads in the future for stability of timberland after afforestation by assessing the factors that affect the damage on the forest road surface and making appropriate constructing standards. Major factors that influence damage to the strip road surface were location, longitudinal gradients, soil types, cross-section shape in order of influence on damage. it is considered that structural road factors like longitudinal gradients, road width, location factors such as construction location, slope gradients and road material like soil types were greatly related to occurrence of road surface damage. Damage occurrences in the forest road were severe at the valley, longitudinal gradients of over 24%, weathered granite soil, concave of road position, road width of over 3.0 m. stability was high at longitudinal gradients of 4~24%, road width of under 3.0 m, ridge of road position, straight slope, soil materials. The evaluation table of damage possibility on forest road was manufactured by discriminant analysis using Quantification theory(II). The results showed that the discriminant ratios was 79.4% and this table was available for forest manager.

• Physical Therapy Korea
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• v.22 no.3
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• pp.98-105
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• 2015

Muscular dystrophy is a hereditary musculoskeletal disorder caused by a mutation in the dystrophin gene. Duchenne muscular dystrophy (DMD) is one of the most common, and progresses relatively faster than other muscular dystrophies. It is characterized by progressive myofiber degeneration, muscle weakness and ultimately ambulatory loss. Since it is an X-linked recessive inheritance, DMD is mostly expressed in males and rarely expressed or less severe in females. The most effective measurement tool for DMD is magnetic resonance imaging (MRI), which allows non-invasive examination of longitudinal measurement. It can detect progressive decline of skeletal muscle size by measuring a maximal cross-sectional area of skeletal muscle. Additionally, other techniques in MRI, like $T_2$-weighted imaging, assess muscle damage, including inflammation, by detecting changes in $T_2$ relaxation time. Current MRI techniques even allow quantification of metabolic differences between affected and non-affected muscles in DMD. There is no current cure, but physical therapist can improve their quality of life by maintaining muscle strength and function, especially if treatment (and other forms of medical intervention) begins in the early stages of the disease.

• Wind and Structures
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• v.2 no.1
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• pp.1-23
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• 1999

Wind effects are critical considerations in the design of topside structures, overall structural systems, or both, depending on the water depth and type of offshore platform. The reliable design of these facilities for oil fields in regions of hostile environment can only be assured through better understanding of the environmental load effects and enhanced response prediction capabilities. This paper summarizes the analysis and performance of offshore platforms under extreme wind loads, including the quantification of wind load effects with focus on wind field characteristics, steady and unsteady loads, gust loading factors, application of wind tunnel tests, and the provisions of the American Petroleum Institute Recommended Practice 2A - Working Stress Design (API RP 2A-WSD) for the construction of offshore structures under the action of wind. A survey of the performance of platforms and satellite structures is provided, and failure mechanisms concerning different damage scenarios during Hurricane Andrew are examined. Guidelines and provisions for improving analysis and design of structures are addressed.

• Journal of Applied Biological Chemistry
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• v.61 no.2
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• pp.109-117
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• 2018

Recently, cancer incidence in modern society is increasing sharply. DNA damage is caused by intrinsic or extrinsic factors in the human body, cells protect themselves by defense mechanism against DNA damage. Also, Aberrant DNA and deficient DNA repair are closely associated with various diseases, including aging and cancer. Researchers are interested in search for proper materials to inhibition for DNA damage. As knew the side effects of synthetic antioxidant, some researches have been conducted about cancer prevention materials derived from nature. Root of Smilax china, in Liliaceae, is used detoxification and tumor treatments traditionally. However, studies on the inhibitory effect of DNA damage haven't progressed. In this study, antioxidant activity and protective effects on oxidative DNA damage of S. china root were confirmed, relationship between those activities and contents of phenolic compounds in plants were established. S. china root effectively removed 1,1-diphenyl-2-picryl-hydrazyl radicals and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid radicals. The quantification and identification of phenolic compounds were confirmed by high performance liquid chromatography analysis, its antioxidant activity was associated with some phenolic compounds. In addition, protective effects against hydroxyl radicals and ferrous ion-induced oxidative DNA damage were confirmed in plasmid DNA. In the cellular levels, S. china root suppressed the expression of ${\gamma}$-H2AX and p53 protein in NIH 3T3. Besides, S. china root suppressed H2AX and p53 mRNA levels. In conclusion, S. china root had the effect on DNA protection and antioxidant.

• Journal of the Korean Society of Safety
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• v.30 no.4
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• pp.174-180
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• 2015

This study developed a Safety Culture Impact Assessment Model (SCIAM) which consists of a safety culture assessment methodology and a safety culture impact quantification methodology. The SCIAM uses safety culture impact index (SCII) to monitor the status of safety culture of the NPPs periodically and it uses relative core damage frequency (RCDF) to present the impact of safety culture on the safety of the NPPs. As a result of applying SCIAM to the reference plant (Kori 3), the standard for the healthy safety culture of the reference plant is suggested. SCIAM might contribute to improve the safety of the NPPs (Nuclear Power Plants) by monitoring the status of safety culture periodically and presenting the standard of healthy safety culture.

• Journal of the Korean Institute of Navigation
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• v.22 no.1
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• pp.23-30
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• 1998

Traffic density has increased recently in Korean waters due to an expansion of the sea trade and the development of coastal fisheries. The enlargement of the coastal industrial belts and the development of coastal inslands further increases marine traffic. The rapid increase of marine traffic has often resulted in marine casualties with the attendant loss of life, damage to property, and marine pollution. Especially, tanker casualties may destroyed the food web and an untold amount of ocean resources. Un regard to the potential of tanker spills in Korean waters, systematic research in this field is lacking. In this paper, the data relating to a total of 261 tanker casualties in Korean waters has been compiled and statistically anlaysed. The result of this study describes the general trend of marine casualties in Korean waters, and describes the casualtiy database, from which their causes and consequences are derived and this results in the determination of the causal relationships connected to tanker casualties, and quantifies the effective level of causal factorsin Korean waters.