• Natural Product Sciences
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• v.22 no.4
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• pp.231-237
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• 2016

Prolonged alcohol consumption causes alcoholic liver damage due to the generation of reactive oxygen species, the accumulation of fatty acids, and an increase in inflammatory cytokines in the liver. In this study, the protective effect of a fruit extract of Paeonia anomala (FEPA) against chronic alcohol-induced liver damage was evaluated in Sprague-Dawley rats fed an ethanol or a control Lieber-DeCarli diet for 5 weeks to induce alcoholic liver damage. FEPA (50, 25, and 10 mg/kg body weight/day) as well as the reference control silymarin (25 mg/kg body weight/day) were administered along with the ethanol diet. FEPA protected against increases in alanine aminotransferase and aspartate aminotransferase in serum and attenuated alcohol-induced increases in triglycerides, tumor necrosis factor alpha, thiobarbituric acid-reactive substances, and cytochrome P450 2E1 enzyme activity in the liver compared with the group treated with ethanol only. Anti-oxidative defenses such as the total glutathione level and glutathione peroxidase activity were increased by FEPA treatment. These results suggest that FEPA exerts protective effects against chronic alcohol-induced liver damage by attenuating hepatosteatosis and pro-inflammatory cytokine production and enhancing anti-oxidative defense mechanisms in the liver.

• Journal of Ocean Engineering and Technology
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• v.33 no.3
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• pp.259-271
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• 2019

It is important to obtain reasonable predictions of the extent of the damage during maritime accidents such as ship collisions and groundings. Many fracture models based on different mechanical backgrounds have been proposed and can be used to estimate the extent of damage involving ductile fracture. The goal of this study was to compare the damage extents provided by some selected fracture models. Instead of performing a new series of material constant calibration tests, the fracture test results for the ship building steel EH36 obtained by Park et al. (2019) were used which included specimens with different geometries such as central hole, pure shear, and notched tensile specimens. The test results were compared with seven ductile fracture surfaces: Johnson-Cook, Cockcroft-Latham-Oh, Bai-Wierzbicki, Modified Mohr-Coulomb, Lou-Huh, Maximum shear stress, and Hosford-Coulomb. The linear damage accumulation law was applied to consider the effect of the loading path on each fracture surface. The Swift-Voce combined constitutive model was used to accurately define the flow stress in a large strain region. The reliability of these simulations was verified by the good agreement between the axial tension force elongation relations captured from the tests and simulations without fracture assignment. The material constants corresponding to each fracture surface were calibrated using an optimization technique with the minimized object function of the residual sum of errors between the simulated and predicted stress triaxiality and load angle parameter values to fracture initiation. The reliabilities of the calibrated material constants of B-W, MMC, L-H, and HC were the best, whereas there was a high residual sum of errors in the case of the MMS, C-L-O, and J-C models. The most accurate fracture predictions for the fracture specimens were made by the B-W, MMC, L-H, and HC models.

• Nutritional Sciences
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• v.6 no.1
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• pp.20-24
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• 2003

The effect of a-tocopherol on the formation and accumulation of phospholipid hydroperoxides, especially of phosphatidylcholine hydroperoxides, in the tissues of 2, 2 -azobis Hydrochloride (AAPH) - dosed rats was investigated. In a-tocopherol supplemented rats, the activities of glutathione peroxidase, catalase and superoxide dismutase were significantly inhibited, compared with the AAPH group. AAPH treatment led to oxidation of phospholipids in the liver, lungs, brain, plasma and red blood cells (RBC), resulting in a notable increase in phosphatidylcholine hydroperoxide (PCOOH). All tissues of the rats given an $\alpha$-tocopherol supplement showed an attenuation of the stimulating effect of AAPH, leading to low levels of formation of PCOOH. Also, the rats injected with AAPH and a-tocopherol showed relatively normal-appearing hepatocytes, except for a little loss of the granules. With regards to the morphological appearance of the liver, it was observed that oral intakes of a -tocopherol resulted in an antioxidant defense against attacks of peroxyl radicals. Thus, we suggest that a-tocopherol is potentially helpful in protecting membrane phospholipids against oxidative damage in vivo.

• Ocean and Polar Research
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• v.24 no.4
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• pp.331-343
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• 2002

The southern tidal flat of Kanghwa Island with an area of approximately $90km^2$ is one of the biggest flats on the west coast of Korea. Surface sediments for sedimentary analyses were sampled at 83 stations in August 1997, September 1999 and August 2000. The very poorly-sorted mud sediments were predominant in the eastern part of the tidal flat, whereas the poorly-sorted sand-mud mixed sediments were dominant in the western part. The area of muddy sediment distribution diminished, but that of sandy mud sediment extended to southeastward tidal flat for three years. In the western part of tidal flat, deposition occurred during the period of spring to summer, whereas erosion occurred in winter. Sediment accumulation rates during three years indicated that the sediments deposited continuously in the eastern part of tidal flat, whereas eroded in the western part of tidal flat. Recently, construction of artificial structures such as new airport, island-connecting bridges and dikes near the tidal flat might change tidal current and river flow pattern. In order to reduce the ecological damage and to preserve tidal-flat environment, it is necessary to Investigate long-term impacts on sedimentary environment and ecology.

• Nuclear Engineering and Technology
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• v.41 no.1
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• pp.11-20
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• 2009

Radiation effects on materials are inherently multiscale phenomena in view of the fact that various processes spanning a broad range of time and length scales are involved. A multiscale modeling approach to embrittlement of pressure vessel steels is presented here. The approach includes an investigation of the mechanisms of defect accumulation, microstructure evolution and the corresponding effects on mechanical properties. An understanding of these phenomena is required to predict the behavior of structural materials under irradiation. We used molecular dynamics (MD) simulations at an atomic scale to study the evolution of high-energy displacement cascade reactions. The MD simulations yield quantitative information on primary damage. Using a database of displacement cascades generated by the MD simulations, we can estimate the accumulation of defects over diffusional length and time scales by applying kinetic Monte Carlo simulations. The evolution of the local microstructure under irradiation is responsible for changes in the physical and mechanical properties of materials. Mechanical property changes in irradiated materials are modeled by dislocation dynamics simulations, which simulate a collective motion of dislocations that interact with the defects. In this paper, we present a multi scale modeling methodology that describes reactor pressure vessel embrittlement in a light water reactor environment.

• Geomechanics and Engineering
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• v.20 no.1
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• pp.55-66
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• 2020

The damage or failure of coal rock is accompanied by energy accumulation, dissipation and release. It is crucial to study the energy evolution characteristics of coal rock for rock mechanics and mining engineering applications. In this paper, coal specimens sourced from the Xinhe mine located in the Jining mining area of China were initially subjected to uniaxial compression, and the micro-parameters of the two-dimensional particle flow code (PFC^2D) model were calibrated according to the experimental test results. Then, the PFC^2D model was used to subject the specimens to substantial uniaxial compression, and the energy evolution laws of coal specimens with various schemes were presented. Finally, the elastic energy storage ratio m was investigated for coal rock, which described the energy conversion in coal specimens with various arrangements of preformed holes. The arrangement of the preformed holes significantly influenced the characteristics of the crack initiation stress and energy in the prepeak stage, whereas the characteristics of the cumulative crack number, failure pattern and elastic strain energy during the loading process were similar. Additionally, the arrangement of the preformed holes altered the proportion of elastic strain energy U_e in the total energy in the prepeak stage, and the probability of rock bursts can be qualitatively predicted.

• Journal of Microbiology and Biotechnology
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• v.27 no.2
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• pp.365-371
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• 2017

Hydrogen has potential for preventive and therapeutic applications as an antioxidant. However, micro- and macroparticles of hydrogen in water disappear easily over time. In order to eliminate reactive oxygen species (ROS) related with the aging process, we used functional water containing nanoparticle hydrogen. Nanoparticle hydrogen does not disappear easily and collapse under water after long periods of time. We used murine embryonic fibroblasts that were isolated from 12.5-day embryos of C57BL/6 mice. We investigated the ability of nanoparticle hydrogen in water to suppress hydroxyurea-induced ROS production, cytotoxicity, and the accumulation of ${\beta}-galactosidase$ (an indicator of aging), and promote cell proliferation. The accumulation of ${\beta}-galactosidase$ in the cytoplasm and the appearance of abnormal nuclei were inhibited by daily treatment of cells with hydrogen water. When the aging process was accelerated by hydroxyurea-induced oxidative stress, the effect of hydrogen water was even more remarkable. Thus, this study showed the antioxidant and anti-senescence effects of hydrogen water. Nanoparticle hydrogen water is potentially a potent anti-aging agent.

• Journal of the Korean Society of Food Science and Nutrition
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• v.23 no.6
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• pp.899-907
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• 1994

Accumulation of peroxidized lipid, fed or injected in the body of rats was investigated and the effect of peroxidized lipid on the antioxidative system was studied also. Three groups each having six of Sprague-dawley rats were raised for 8 weeks. the peroxide value(POV) of diet fed to the control and the peroxidized group was 5.47 and 22.14meq/kg , respectively. Injected group was given the control diet and peroxidized linoleic acid(POV 31.81meq/kg) was injected into the peritoneal area three times a week. The POV, MDA, and protein carbonyl values of the peroxidized and the injected group (experimental groups) were significantly higher (p<0.05) than those of the control group. Cu, Zn-SOD and M-SOD activity of the experimentla groups increased 1.6 times that of control group at 4 th week. and decreased by 60% of their activityafter 8 weeks of feeding (p<0.05) . Catalase activity, glutathione and Vt, E contents of the experimental groups were significantly lower (p<0.05) than those of the control group during 8 weeks. The accumulation of peroxidcized lipid in liver were ovserved both in the fed or the injected group. The increased of enzyme activity of the experimental group during 4 weeks suggests ianadaptation of antioxidative system to get rid of the peroxidized lipid. Decrease of enzyme activity and glutathione observed as the peroxidized lipid lipid accumulation proceeded further, however, seems to indicate the oxdiative damage of enzyme and protein . Determination of the protein carbonyl content may be used as a method for measuring the oxidative damaging effect of peroxidized lipid.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.6
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• pp.490-497
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• 2020

Asphalt pavement accounts for more than 90% of the total pavement in Korea. Pavement is most widely constructed among construction structures. The heat transfer characteristics (Thermophysical Properties) of the asphalt pavement cause the heat island effect in downtown areas. An increasing asphalt surface temperature is one of the major causes of damage to asphalt pavement. This study examined the heat transfer characteristic factors according to solar energy accumulation in an asphalt mixture. The specimens (WC-2 & PA-13, Recycled aggregate used WC-2) used in the experiment were compacted with a Gyratory Compactor. The thermo-physical properties (thermal conductivity, specific heat capacity, thermal diffusivity, and thermal emissivity) and solar energy accumulation were evaluated. The thermal accumulation and HFM tests revealed a 1.2- to 2.0-fold difference. This indicates that the thermal conductivity of the asphalt mixture pavement changes with the accumulation of solar energy. An analysis of the correlation of thermal conductivity according to the surface temperature of the asphalt mixture showed that WC-2 was logarithmic, and PA-13 was linear. Experiments on the heat transfer characteristics of asphalt pavement that can be used for thermal failure modeling of asphalt were conducted.

• CELLMED
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• v.2 no.3
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• pp.26.1-26.5
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• 2012

Adverse side-effects and lack of scientific validation of some chemotherapeutic agents prevent the use of many traditional medicines claimed to have anti-cancer effects. Ethanolic extract of Conium maculatum has long been used in traditional and alternative systems of medicine including homeopathy for the treatment of glandular enlargements, cancerous tumours or hard lumps of testicles, prostate, ovaries, breasts and/ or uterus, particularly in the breast. However, if and how it acts still remains scientifically unknown. This study aims to test if Conium extract (CE), used as mother tincture of Conium in homeopathy, has demonstrable anti-cancer potentials without having much cytotoxicity in normal cells. Cytotoxicity of the drug was tested by conducting MTT assay on both normal (peripheral blood mononuclear cells) and HeLa cells. We also evaluated DNA fragmentation and DNA damage by DAPI and diphenylamine assay. The LDH activity assay was done to evaluate the percentages of apoptosis and necrosis. ROS accumulation also was evaluated to pin-point the actual events of apoptosis. Administration of drug clearly demonstrated its anti-cancer potentials as evidenced by the DNA damage analysis. The ROS activity also increased in case of the CE treated cells. LDH data revealed that the mode of cell death was mainly apoptotic and not necrotic. CE appears to induce apoptosis of cancer cells through ROS mediated pathway, and has negligible cytotoxicity against normal cells.