• Title/Summary/Keyword: daf-16

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Changes in Development and Nutrient Composition of Pod after Flowering in Cowpea 〔Vigna unguiculata(L) Walp〕 (동부(Vigna unguiculatal(L) Walp)의 개화후 일수경과에 따른 협실비대 및 품질의 변화)

  • 김수동;차영훈;조진태;권규칠;손삼곤;박상일
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.31 no.1
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    • pp.68-73
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    • 1986
  • In order to identify the physiological maturity and to determine the proper time of harvesting as fresh seed for cooking with rice and germination ability according to the harvesting time in cowpea (Vigna unguiculata (L.) Walp), this experiment was conducted. The results obtained are as follows; 1. The length and width of pods became maximum size on 10 days after flowering(DAF) and thickness of them increased to 14 DAF and then decreased to 18 DAF. 2. The weight of pod wall reached the maximum on 14 DAF, the thickness of them increased to 8 DAF and decreased to 18 DAF and it was stabilized. 3. The length and thickness of the seeds became larger to 10 DAF, were not changed from 10 DAF to 16 DAF and then decreased after 16 DAF. 4. The seeds coloured 2 days earlier than the pod wall on 8 DAF and the grade increased by degrees to 16 DAF. 5. The moisture content of seeds and pod wall started to decrease on 8 DAF and the moisture content of pod wall was higher than that of seeds in the middle stage but the condition changed after 18 DAF. 6. When the weight of seeds reached the maximum on 16 DAF, the moisture content of them was 54.5%. 7. The content of total nitrogen, phosphate and oil decreased but the carbohydrate increased by degrees along the passing of DAF. The silicic acid did not exist and K$_2$O, CaO, MgO, protein etc. existed but did not show regular tendency. 8. The physiological maturity was 16 DAF and the proper time of harvesting as fresh seed for cooking with rice was 12 DAF to 16 DAF. 9. Germination was possible after 10 DAF but we could get the normal roots from the seeds harvested after 14 DAF.

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Thermal Resistance and daf-16 Regulation of Fermented Zizyphus jujuba Fruits in Caenorhabditis elegans

  • Yu, Young-Bob
    • Korean Journal of Plant Resources
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    • v.33 no.6
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    • pp.645-650
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    • 2020
  • The mechanism of anti-aging of fermented jujube (Zizyphus jujuba fruits (ZJF)) was investigated using transgenic daf-16 and mev-1 strains of C. elegans. Jujube extracts fermented for 7 days (F7-ZJF) and 14 days (F14-ZJF) with Laetiporus sulphureus were treated to a NGM agar plate with 10-15 transgenic daf-16 and mev-1 strains of the synchronized age. There was no difference of lifespan between the drug-treated group (7-day fermented ex. (F7-zjf-200 ㎍/mL), 14-day fermented ex. (F14-zjf-200 ㎍/mL)) and the non-treatment group in both daf-16 and mev-1 strains. In the thermal stress experiment, F7-zjf-200 ㎍/mL showed a significant (t = 4.017) activity in thermal stress resistance with a 12% higher survival rate than the control group. In the survival test in H2O2, F7-zjf-200 ㎍/mL and F14-zjf-100 ㎍/mL have significant activity in oxidative stress resistance compared to the control group. This study indicates that life span expand of N2 strain of the jujube extract is related to the regulation of daf-16 and inhibition of mev-1 signal in C. elegans.

The Design and Implementation of Ontology for Simulation based Architecture Framework (ONT-AF) in Military Domain (SBA AF의 구축을 지원하는 온톨로지의 설계 및 구현(ONT-SAF))

  • Kwon, Youngmin;Sohn, Mye;Lee, Wookey
    • Journal of Information Technology and Architecture
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    • v.9 no.3
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    • pp.233-241
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    • 2012
  • Architecture framework (AF) is a guideline to define components needed to develop and operate enterprise architecture (EA), and to define relationships among the components. There are many architecture frameworks to operate EA of governments and businesses such as Zachman framework, DoDAF, TOGAF, FEAF, and TEAF. DoDAF is the most representative AF to support the development of the EA in the military domain. DoDAF is composed of eight viewpoints and 40 views that are affiliated with the viewpoints. To develop an AF for a specific goal, system architects decide a set of views. Furthermore, they determine data that are needed for a view modeling. However, views and data in DoDAF are structurally inter-related explicitly and/or implicitly. So, developing an AF for a specific goal is going to be a project to be carried out over a long haul. To reduce the burden of its development, in this paper, we develop ONT-SAF (Ontology for DoDAF) that can infer inter-relationships like referential and transitive relationships and the sequences among the views. Furthermore, to promote reusability and consistency of the views and the data within an AF, we adopt the view-data separation strategy. ONT-DAT contains classes like 'viewpoint', 'view', 'data', 'expression method', and 'reference model', and 11 properties including 'hasView.' To prove the effectiveness of ONT-SAF, we perform a case study.

Treatability Study on the Remediation Groundwater Contaminated by TPH Cr6+ : Lab-Scale Experiment (TPH와 6가 크롬으로 오염된 지하수 처리를 위한 실내 실험)

  • Lee, Gyu-Beom;Chang, Yoon-Young
    • Journal of Environmental Impact Assessment
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    • v.28 no.3
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    • pp.332-345
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    • 2019
  • The purpose of the study is to evaluate the treatability of contaminated groundwater with TPH and (or) $Cr^{6+}$. Laboratory scale tests were performed for oil/water separation, dissolved air flotation (DAF), coagulation and precipitation, and filtration with sand and activated carbon respectively. Two times of oil/water separation tests for total 40 minutes of separation or separating time shows 90.2 % of TPH removal rate. In case of DAF test for high TPH sample, the TPH removal rates were not varied significantly by the variation of microbubble size. However, tests for low TPH samples show that TPH removal rate increases as microbubbles are smaller. When coagulant was added to sample for DAF test, TPH removal rate was increased 12.3 %. SS removal rate by DAF was 97.9 % at $16-40{\mu}m$ and it was increased as the size of microbubble is reduced. Tests for coagulation and precipitation were performed to evaluate the removal of $Cr^{6+}$ in groundwater. The increase of $FeSO_4$ dosage increased $Cr^{6+}$ removal rate in the coagulation and precipitation process. As the amount of activated carbon in the filter media increased TPH removal rate in the filtration process. SS removal rate by the filtration was 96.7 % similar to the results of DAF process tests. The filtration process treats TPH and SS. Best design parameters are determined as the size of sand is $425-850{\mu}m$ and the ratio of activated carbon and sand is 50:50.

Infection and Immune Response in the Nematode Caenorhabditis elegans Elicited by the Phytopathogen Xanthomonas

  • Bai, Yanli;Zhi, Dejuan;Li, Chanhe;Liu, Dongling;Zhang, Juan;Tian, Jing;Wang, Xin;Ren, Hui;Li, Hongyu
    • Journal of Microbiology and Biotechnology
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    • v.24 no.9
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    • pp.1269-1279
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    • 2014
  • Xanthomonas oryzae pv. oryzae (Xoo) strains are plant pathogenic bacteria that can cause serious blight of rice, and their virulence towards plant host is complex, making it difficult to be elucidated. Caenorhabditis elegans has been used as a powerful model organism to simplify the host and pathogen system. However, whether the C. elegans is feasible for studying plant pathogens such as Xoo has not been explored. In the present work, we report that Xoo strains PXO99 and JXOIII reduce the lifespan of worms not through acute toxicity, but in an infectious manner; pathogens proliferate and persist in the intestinal lumen to cause marked anterior intestine distension. In addition, Xoo triggers (i) the p38 MAPK signal pathway to upregulate its downstream C17H12.8 expression, and (ii) the DAF-2/DAF-16 pathway to upregulate its downstream gene expressions of mtl-1 and sod-3 under the condition of daf-2 mutation. Our findings suggest that C. elegans can be used as a model to evaluate the virulence of Xoo phytopathogens to host.

Moringa oleifera Prolongs Lifespan via DAF-16/FOXO Transcriptional Factor in Caenorhabditis elegans

  • Im, Jun Sang;Lee, Ha Na;Oh, Jong Woo;Yoon, Young Jin;Park, Jin Suck;Park, Ji Won;Kim, Jung Hoon;Kim, Yong Sung;Cha, Dong Seok;Jeon, Hoon
    • Natural Product Sciences
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    • v.22 no.3
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    • pp.201-208
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    • 2016
  • Here in this study, we investigated the lifespan-extending effect and underlying mechanism of methanolic extract of Moringa olelifa leaves (MML) using Caenorhabditis elegans (C. elegans) model system. To define the longevity properties of MML we conducted lifespan assay and MML showed significant increase in lifespan under normal culture condition. In addition, MML elevated stress tolerance of C. elegans to endure against thermal, oxidative and osmotic stress conditions. Our data also revealed that increased activities of antioxidant enzymes and expressions of stress resistance proteins were attributed to MML-mediated enhanced stress resistance. We further investigated the involvement of MML on the aging-related factors such as growth, food intake, fertility, and motility. Interestingly, MML significantly reduced growth and egg-laying, suggesting these factors were closely linked with MML-mediated longevity. We also observed the movement of aged worms to estimate the effects of MML on the health span. Herein, MML efficiently elevated motility of aged worms, indicating MML may affect health span as well as lifespan. Our genetic analysis using knockout mutants showed that lifespan-extension activity of MML was interconnected with several genes such as skn-1, sir-2.1, daf-2, age-1 and daf-16. Based on these results, we could conclude that MML prolongs the lifespan of worms via activation of SKN-1 and SIR-2.1 and inhibition of insulin/IGF pathway, followed by DAF-16 activation.

Hydrodynamic Collision Efficiency and Flotation Characteristics of Inorganic Particles in DAF Process (DAF 공정에서 무기 고형입자의 유체역학적 충돌효율과 부상특성)

  • Kwak, Dong-Heui;Kim, Sung-Jin;Lee, Hwa-Kyung;Jung, Heung-Joe;Lee, Jae-Wook;Chung, Paul-Gene
    • Journal of Korean Society of Water and Wastewater
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    • v.16 no.6
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    • pp.655-662
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    • 2002
  • Separation characteristics of inorganic particles occurred during heavy rainwater were investigated in DAF (dissolved air flotation) process. In order to remove the inorganic particles effectively, the collision and flotation efficiencies were examined from a hydrodynamic point of view. Generally, the collision efficiency increased with floc size under the variation of fluid dynamic conditions including inertial force. However, more precise model should be required to analysis the collision efficiency expressed both the physical properties for inorganic particles and hydrodynamic conditions for a reactor.

Decay-Accelerating Factor Differentially Associates With Complement-Mediated Damage in Synovium After Meniscus Tear as Compared to Anterior Cruciate Ligament Injury

  • V. Michael Holers;Rachel M. Frank;Michael Zuscik;Carson Keeter;Robert I. Scheinman;Christopher Striebich;Dmitri Simberg;Michael R. Clay;Larry W. Moreland;Nirmal K. Banda
    • IMMUNE NETWORK
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    • v.24 no.2
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    • pp.17.1-17.16
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    • 2024
  • We have reported that anterior cruciate ligament (ACL) injury leads to the differential dysregulation of the complement system in the synovium as compared to meniscus tear (MT) and proposed this as a mechanism for a greater post-injury prevalence of post traumatic osteoarthritis (PTOA). To explore additional roles of complement proteins and regulators, we determined the presence of decay-accelerating factor (DAF), C5b, and membrane attack complexes (MACs, C5b-9) in discarded surgical synovial tissue (DSST) collected during arthroscopic ACL reconstructive surgery, MT-related meniscectomy, osteoarthritis (OA)-related knee replacement surgery and normal controls. Multiplexed immunohistochemistry was used to detect and quantify complement proteins. To explore the involvement of body mass index (BMI), after these 2 injuries, we examined correlations among DAF, C5b, MAC and BMI. Using these approaches, we found that synovial cells after ACL injury expressed a significantly lower level of DAF as compared to MT (p<0.049). In contrast, C5b staining synovial cells were significantly higher after ACL injury (p<0.0009) and in OA DSST (p<0.039) compared to MT. Interestingly, there were significantly positive correlations between DAF & C5b (r=0.75, p<0.018) and DAF & C5b (r=0.64 p<0.022) after ACL injury and MT, respectively. The data support that DAF, which should normally dampen C5b deposition due to its regulatory activities on C3/C5 convertases, does not appear to exhibit that function in inflamed synovia following either ACL injury or MT. Ineffective DAF regulation may be an additional mechanism by which relatively uncontrolled complement activation damages tissue in these injury states.

Variation of Sink Components in Response to Removal Time of Upper Leaf on Main Stem in Soybean (주경 상부엽 제거시기가 콩의 절위별 Sink형질 변이에 미치는 영향)

  • 박춘봉;이중호
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.1
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    • pp.16-25
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    • 1995
  • The effects of leaf removal time on variation of nodal sink components in determinated soybean [Glycine max (L.) Merr.] cultivar 'Danyeobkong' were measured at the experiment field of Chonbuk Provincial Rural Development Administration in 1991. Node order in this experiment was calculated from terminal node to bottom node to clarify the photosynthetic ability of canopy leaves. The upper 5 leaves from terminal node of main stem were removed at 5, 15, 25, 35 and 45 days after flowering(DAF) respectively. In spite of light-receiving increment in lower part of main stem and in branch by removing the upper part leaves of main stem, seed weight of that part was not increased in leaf removal treatment compared with control block. The node position reducing pod went down from terminal to bottom by delaying leaf removal time, and the influence of pod number reduction was bigger in DAF 25 than in DAF 15. But the seed number reduction per pod was the biggest in DAF 35. Cracked seed coat ratio ranged from 25% to 35% in control block, while that of leaf removal block had mostly 10% gap compared with the control block.rol block.

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