• 제목/요약/키워드: cytochrome P450 (P450)

검색결과 942건 처리시간 0.024초

Formalin에 노출시킨 넙치 (Paralichthys olivaceus) 간장 약물대사효소의 in vivo 및 in vitro 반응 (In vitro and In vivo Responses of Hepatic Xenobiotic Metabolizing Enzymes in Flounder (Paralichthys olivaceus) Exposed to Formalin)

  • 이지선;하진환;이경선;전중균
    • 환경생물
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    • 제24권2호
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    • pp.195-200
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    • 2006
  • The response of hepatic mixed function oxygenase (MFO) system was investigated in olive flounder exposed to formalin. Hepatic microsome of olive flounder incubated in vitro with formalin demonstrated the induction of cytochrome P450 (CYP), ethoxyresorufin deethylase (EROD), cytochrome P450 reductase (P450R) and cytochrome b5 reductase (b5R) activity. In addition, olive flounder was exposed to 100, 300 and 500 ppm of formalin for 1 h and then transferred to a flow-through type of 1000 L aquarium. Hepatic MFO enzyme activity was determined for 72 h. As the result, hepatic CYP, P450R and EROD activities increased following exposure of formalin, but b5R and GST showed no significant change. These results imply that CYP and P450R can be considered as main hepatic enzymes involving in detoxification of formalin.

먹이종류에 따른 몇가지 곤충의 MFO활성 비교 (Activity of Mixed Function Oxidase in a few Insect Species in Relation to Their Food Source)

  • 이정호;부경생
    • 한국응용곤충학회지
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    • 제32권3호
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    • pp.291-299
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    • 1993
  • 네가지 종류의 곤충 중장에 있는 주요 해독효소인 cytochrome P-450 monooxygenases의 활성을 그들의 먹이종류 및 섭식여부와 연관시켜 분석하였다. 담배나방유충을 인공사료로 사육하였을 때 전반적으로 중장의 cytochrome P-450 monooxygtenases(MFO)활성이 지방체의 경우보다 3배가량 높게 나타났다. 고추열매나 인공사료를 공급하여 사육하였을 때보다 담배잎으로 사육하였을 때 담배나방유충의 중장 MFO는 훨씬 높게 나타났으며, 흰불나방의 경우염주나무잎이 MFO활성을 가장 높게 유기했다. 담배나방, 파밤나방, 희불나방, 담배거세미나방 등의 유충들을 각각의 인공사료로 사육하였을 때 광식성이며 동시에 살충제저항성을 보유하고 있는 파밤나방의 MFO활성이 가장 높게 나타났다.

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인삼이 백서 간 약물대사효소에 미치는 효과 (The Effect of Ginseng on Hepatic Drug Metabolizing Enzyme in Rats)

  • 이태하;김낙두
    • 약학회지
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    • 제25권4호
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    • pp.145-151
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    • 1981
  • The effect of ginseng methanol extract on hepatic drug metabolizing enzyme in rat was investigated. The ginseng methanol extract (100mg/kg) was administered orally to Sprague Dawley rats for 7days and the contents of cytochrome $P_{450}$ and NADPH cytochrome c reductase in liver were measured by the method of Stanton et al. and Mazel respectively. The content of liver cytochrome $P_{450}$ and NADPH cytochrome c reductase in the rats treated with ginseng methanol extract (100mg/kg) were increased by 21.9% and l6.6% respectively and their increases were statistically significant. Single i.p. injection of phenobarbital (100mg/kg) to the rats produced approximately 25% increase in cytochrome $P_{450}$ content in this investigation and further stimulation was produced in the rats pretreated with ginseng methanol extract (100mg/kg). On the other hand, single i.p. injection of 95% $CCl_{4}$ (0.5ml/kg) showed 29% decrease in cytochrome $P_{450}$ content and 10.5% decrease in NADPH cytochrome c reductase activity. The degree of inhibition of cytochrome $P_{450}$ content in the rats pretreated with ginseng methanol extract (100mg/kg) was similar to that observed in the $CCl_{4}$ alone treated group, but NADPH cytochrome c reductase activity was increased by 65% in the rats pretreated with ginseng methanol extract (100mg/kg). These results suggest that ginseng is the hepatic drug metabolizing enzyme inducing agent in the rat and the effect is similar to phenobarbital.

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Expression and Characterization of Truncated Recombinant Human Cytochrome P450 2J2

  • Park, Hyoung-Goo;Lim, Young-Ran;Han, Songhee;Kim, Donghak
    • Toxicological Research
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    • 제30권1호
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    • pp.33-38
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    • 2014
  • The human cytochrome P450 2J2 catalyzes an epoxygenase reaction to oxidize various fatty acids including arachidonic acid. In this study, three recombinant enzyme constructs of P450 2J2 were heterologously expressed in Escherichia coli and their P450 proteins were successfully purified using a $Ni^{2+}$-NTA affinity column. Deletion of 34 amino acid residues in N-terminus of P450 2J2 enzyme (2J2-D) produced the soluble enzyme located in the cytosolic fraction. The enzymatic analysis of this truncated protein indicated the typical spectral characteristics and functional properties of P450 2J2 enzyme. P450 2J2-D enzymes from soluble fraction catalyzed the oxidation reaction of terfenadine to the hydroxylated product. However, P450 2J2-D enzymes from membrane fraction did not support the P450 oxidation reaction although it displayed the characteristic CO-binding spectrum of P450. Our finding of these features in the N-terminal modified P450 2J2 enzyme could help understand the biological functions and the metabolic roles of P450 2J2 enzyme and make the crystallographic analysis of the P450 2J2 structure feasible for future studies.

($\pm$)-Camphor가 ICR 마우스 수컷의 간 cytochrome P450 효소 활성에 미치는 영향 (Effects of($\pm$)-camphor on Hepatic Cytochrome P450 Enzymes in Male ICR Mice)

  • 오은경;박형건;배기헌;최옥진;최은경;최창근;한진희;정태천
    • Environmental Analysis Health and Toxicology
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    • 제17권4호
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    • pp.307-314
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    • 2002
  • Effects of ($\pm$)-camphor on liver cytochrome P450 enzymes were investigated in male ICR mice. Mice were treated intraperitoneally with 0, 200, 400 and 800 mg/kg of ($\pm$)-camphor in corn oil for 3 consecutive days. Twenty four hr after the final treatment, the animals were subjected to necropsy. The activities of serum aspartate aminotransferase and serum alanine aminotransferase were slightly changed by the treatment with ($\pm$)-camphor at the doses used. Administration of ($\pm$)-camphor to mice significantly induced the hepatic activities of pentoxyresorufin O-depentylase and benzyloxyresorufin O-debenzylase and weakly induced ethoxyresorufin O-deethylase in dose-dependent manners. The present results suggested that ($\pm$)-camphor might act as a relatively specific inducer of hepatic cytochrome P450 2B in male ICR mice.

Tryptophan 투여가 Reserpine과 식이 탄수화물 수준이 다른 저단백식이를 섭취한 흰쥐의 혈장 아미노산 농도, 간 Cytochrome P450 함량 및 간세표 미세구조에 미치는 영향 (The Effect of Tryptophan Administration on the Plasma Free Amino Acid Concentration, Liver Microsomal Cytochrome P450 Content and Cellular Structure of Rats Consumed Reserpine and Low Protein Diet with Different Carbohydrate Contents)

  • 신동순
    • Journal of Nutrition and Health
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    • 제29권7호
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    • pp.689-702
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    • 1996
  • The purpose of this experiment was to compare the effects of tryptophan administration on nutritional status of female rats which consumed reserpine and 6% casein diet with different carbohydrate contents(87%, 65%, 44% respective). Final body weight, body weight gain, FER, plasma amino acid concentration and microsomal cytochrome P 450 content in liver were measured and microscopic structure of hepatocytes was observed. In low-protein diet, the higher the carbohydrate content of diet was, the lower the damage was in the rat's liver. Tryptophan administration after dose of reserpine induced more effective recovery from liver damage of rats in high carbohydrate diet group than that in low carbohydrate diet group. In conclusion, the general nutritional assessments such as final body weight and body weight gain provided better estimate of the degree of structural changes in hepatocytes than functional assessment such as plasma amino acid concentration or liver microsomal cytochrome P450.

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Role of Cytochrome P-450 in the Bioactivation of Nicotine

  • Kim, Bong-Hee;Anthony Travor
    • Archives of Pharmacal Research
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    • 제14권2호
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    • pp.130-136
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    • 1991
  • Nicotine (100 .mu. M) was incubated with microsomes (1 mg/ml) prepared from New Zealand White rabbits. On the basis of microsomal weight, the rate of nicotine oxidation were calculated on the basis of cytochrome P-450 concentration, the specific activity of the metabolic oxidation catalyzed by lung was approximately 4 times greater than liver (6.4 vs 1, 65 nmoles nicotine oxidized. nmole cytochrome $P-450^{-1}\;min{-1})$. These studies employed several methods of altering activities of specific isozymes present in pulmonary microsomes, including the use of the isozyme2 and 6 specific inhibitor $\alpha$-methylbenzyl ABT, metabolite inhibitors, norbenzphetamine and N-hydroxyamphetamine. TCDD induction and Arochlor 1260 pretreatment. These results support the conclusion that nicotine metabolism by rabbit lung microsomes is mediated primarily by cytochrome P-450 isozyme 2.

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방선균 시토크롬 P450와 전자전이시스템 (Streptomyces Cytochrome P450 and Electron Transport System)

  • 송재경;오태진
    • 한국미생물·생명공학회지
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    • 제38권3호
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    • pp.227-234
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    • 2010
  • Cytochrome P450 enzymes which require the supply of electrons from NAD(P)H have a great biotechnological impact as they catalyze valuable reactions on a vast variety of substrates. However, very limited biotechnological application has been reported so far due to their functional complexity, limited stability (instability) and, in most cases, low catalytic activity. In this present review, we introduce some possibilities for improving their defect by exploring electron transport system and substrate flexibility in field of Streptomyces cytochrome P450.

A Study for Regulation of Ethanol-inducible $P_{450}$(CYP2E1) on $CCI_4$-induced Hepatic Damage

  • Park, Sun-Mi;Park, Eun-Jeon;Ko, Geon-Il;Kim, Jae-Baek;Sohn, Dong-Hwan
    • Archives of Pharmacal Research
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    • 제18권3호
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    • pp.179-182
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    • 1995
  • Previous study showed that $CCl_4$ administration evoked a rapid decrease in cytochrome $P_{450}$ 2E1 protein soon after the exposure due to posttranslational inhibition(Biochem. Biophys. Res. Commun. 179:449-454, 1991). In this report, aniline hydroxylase and the amounts of immunoreactive $P_{450}$ 2E1 were rapidly decreased during day 1 to 2 and recovered during day 3 to 4 after a single dose of $CCl_4$. The activity of pentoxyresorufin-O-dealkylase was also suppressed at day 1 and began to repair from day 2. However, the decrease in immunoreactive $P_{450}$ 2C content was not observed. The decreases in $P_{450}$ 2E1 enzyme activity and immunoreactive protein by acute $CCl_4$ treatment were accompanied by a decline in $P_{450}$ 2E1 mRNA level. The data thus suggested a pretranslational reduction of $P_{450}$ 2E1 during day 1 to 2 after acute $CCl_4$ treatment.

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