• The Korean Journal of Pesticide Science
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• v.14 no.3
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• pp.247-254
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• 2010

Two-spotted spider mite, Tetranychus urticae was collected from the rose greenhouse in Chilgok, Gyeongbuk Province in December 2000. This population has been selected for ten years with bifenazate (over 450 times), and increased 855.9 fold in resistance as compared with susceptible strain (S). Cross resistance of bifenazate resistant (BR) strain to eight miticides was investigated. The BR strain exhibited high and low cross resistance to acequinocyl (614.0 fold) and to chlorfenapyr (9.1 fold), respectively. Against fenazaquin (0.3 fold) and fenpyroximate (0.1 fold), however, showed the strain negatively correlated cross resistance. Each strain collected in Choeng-ju (CJ), Kang-jin (KJ), and Chung-ju (CUJ) showed 5.5-, 964.5-, and 21.8-fold resistance to bifenazate, respectively. The detoxifying enzymes of the BR strain showed 1.6-fold activity in cytochrome $P_{450}$-dependent monooxygenase ($P_{450}$) as compared with susceptible one. By comparing the mitochondrial cytochrome b (cytb) sequence, G126S point mutation was detected in the BR and KJ strains.

• Journal of Marine Life Science
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• v.1 no.1
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• pp.8-17
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• 2016

The family Pristiophoridae belonging to the order Pristiophoriformes consists of about 8 species and 2 genera in the world. Among them, only one species (Pristiophorus japonicus) is distributed in northwestern Pacific. The family is characterized by having an elongated and flattened rostrum with laterally projecting a low of sharp teeth. In order to clarify variability of local populations of P. japonicus, we investigated 16 individuals collected from four locations (Korea, n=6; Miyagi, n=1; Kochi Pref., n=1; Okinawa, n=8) for morphological and molecular analyses. Morphological analysis showed that there may exist three morphotypes, of which A morphotype (Korea, Miyagi, Kochi Pref.) has a short snout (26.8% of TL), being most similar to syntypes of P. japonicus. On the other hand, B morphotype (Okinawa) has a long (31.7% of TL) and wider snout (snout width at nostrils 5.2 times pre-oral length) and, C morphotype (Okinawa) has a long (31.7% of TL) and narrower snout (6.3 times). Molecular analysis using mitochondrial DNA cytochrome b DNA sequences (386 base pairs) showed that the K2P distance between A and C morphotypes were 2.1~2.7%, but those within A morphotype were 0.0~0.5%. We identified A morphotype as P. japonicus, and designated B and C morphotypes as Pristiophorus sp. 1 and Pristiophorus sp. 2, respectively. Further studies are required to elucidate the taxonomic status of two morphotypes of the sawshark in Okinawa.