• 한국미생물·생명공학회지
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• 제21권2호
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• pp.119-124
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• 1993

An alkalophilic microorganism for overproduction of cyclodextrin glucanotransferase (CGTase) was newly isolated from hot-water spring soil, and identified as Bacillus firmus var. alkalophilus H609. The strain maintained stability during preservation and cultivation for the enzyme production, and produced significant amount of CGTase corresponding to the volumetric activity of 75 units/mL at 37C, initial pH of 11.2, and after 40 hours. The strain excreted several different proteins showing CGTase activity that catalyzed the formation of mainly beta-and Gamma-type cyclodextrin (ratio of 7:1) from soluble starch without accumulation of alpha-type. Other enzymatic properties were also investigated.

• Journal of Pharmaceutical Investigation
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• 제14권3호
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• pp.136-143
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• 1984

Inclusion complex formation of fenbufen with ${\beta}-cyclodextrin$ in water and in solid state was comfirmed by solubility method, ultra violet absorption, circular dichroism and infra-red spectroscopies, differential thermal analysis, and X-ray diffractometry. A solid complex of fenbufen with ${\beta}-cyclodextrin$ in 1 : 1 molar ratio was prepared by the freeze-drying method, its dissolution characteristics in water and its analgesic and antiinflammatory effect in mouse or rat were examined. The apparent release of fenbufen from the inclusion complex was significantly improved, hut no significant difference in its analgesic and antiinflammatory effect was found.

• 분석과학
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• 제15권2호
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• pp.115-119
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• 2002

$\alpha$ 및 $\beta$-Cyclodextrin(CD)과 Congo red간의 복합체 형성에 관한 연구를 분광학적 방법으로 조사하였다. 이 결과 cavity 크기가 작은 $\alpha$-CD는 Congo red의 입체적 장애 때문에 복합체를 형성하지 못하였다. 한편 가시광선 영역의 S-형 흡광도 증가 현상으로 볼 때 두 분자의 $\beta$-CD가 한 분자의 Congo red와 equatorial 형태로 복합체를 형성하는 것 같다. 등 흡수점은 346 nm과 478 nm 두 곳에서 나타났다. 온도에 따른 형성상수의 값으로부터 온도가 올라가면 binding energy의 감소로 형성상수가 감소하였다. 열역학적 parameter의 계산 결과 $\Delta$H, $\Delta$S 및 $\Delta$G는 각각 -50.73 kJ/mol, $-108.96J/K{\cdot}mol$ 및 -18.24 kJ/mol 이었다. 따라서 큰 엔트로피 감소를 동반한 자발적 반응임을 알 수 있었다.

• 한국미생물·생명공학회지
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• 제23권4호
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• pp.425-431
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• 1995

A kinetic model of the cyclodextrin formation in a heterogeneous enzyme reaction system using swollen extrusion starch as substrate was derived emphasing the structural features of extrusion starch. The degree of gelatinization, the ratio of accessible and inaccessible portion of extrusion starch, adsorption of CGTase on swollen starch, the structural transformation during reaction, and product inhibition caused by produced CDs were considered in deriving kinetic model. Various kinetic constants were also evaluated. The derived kinetic equation was numerically simulated, which result showed that the derived kinetic equations can be used to predict the experimental data reasonably well under the various experimental conditions. Kinetic model can be utilized for the optimization of enzyme reactor and the process development for CD production from swollen extrusion starch.

• Journal of Pharmaceutical Investigation
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• 제17권2호
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• pp.75-78
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• 1987

The release rate of heparin from monolithic devices composed of raffinose, ${\beta}-cyclodextrin$, polyethylene oxide (Mw 20,000, PEO), and hydrophobic polyether urethane (biomer) was investigated. Water soluble raffinose, ${\beta}-cyclodextrin$, and PEO blended into the biomer provided a controlled release of heparin. The release rate of heparin could be controlled by the content of raffinose, ${\beta}-cyclodextrin$, and PEO in the devices. The mechanism of release rate increased by the raffinose, ${\beta}-cyclodextrin$, and PEO may result from the formation of channels and pores in the biomer matrices following the swelling and the change in the physical structure of polymer net work. Hydrophobic polyurethane containing raffinose, ${\beta}-cyclodextrin$, and PEO can provide a hydrophilic antithrombogenic material for prolonged release of heparin.

• 공업화학
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• 제18권4호
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• pp.326-329
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• 2007

수용액 상에서 ${\beta}$-cyclodextrin에 2,4'-dibromoacetophenone (8)을 반응시켜 착물을 형성한 후 arylidenethiosemicarbazone 7a-f를 가하여 2-arylidenehydrazinyl-4-arylthiazole 유도체들 9a-f를 70~88% 수율로 합성하였다. 합성한 새로운 화합물들 9a-f의 구조를 IR과 $^1H-NMR$ 스펙트럼 자료로 확인하였다. 이와 같은 실험결과 ${\beta}$-cyclodextrin의 역할은 반응물 7 및 8의 활성화로 반응을 촉진시켜 반응시간이 단축되는 것으로 나타났다.

• Bulletin of the Korean Chemical Society
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• 제34권7호
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• pp.2016-2022
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• 2013

Ionically modified ${\beta}$-cyclodextrins, which have excellent water-solubility, have been interested in purification technology as well as drug carrier system. The present study summarizes the synthesis and characterization of cationic and anionic ${\beta}$-cyclodextrin (${\beta}$-CyD) products using by polycondensation. The oligo (${\beta}$-CyD)s are synthesized from ${\beta}$-CyD, epichlorohydrin (EP) and choline chloride (CC; for cationic polymer) or chloroacetic acid (CAA; for anionic polymer) through one step polycondenstaion process. Unlike the previous studies, we successfully purified the ionic ${\beta}$-CyD condensation products from the ${\beta}$-CyD reaction mixtures and accomplished a great level of structural analysis. The detailed structural analysis of these ionic ${\beta}$-CyD compounds is done by $^1H$ NMR, MALDI-TOF as well as GPC analysis and confirms the formation of oligomers with a few units of ${\beta}$-CyD. We found that the sequence of reactant addition also could effect on the molecular weight of the resulting product as well as the molar ratio of the reactants. Finally, we used the cationic and anionic ${\beta}$-CyD oligomers for fabricating multilayer films by layer-layer process.

• Journal of Pharmaceutical Investigation
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• 제13권1호
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• pp.10-22
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• 1983

The interactions of ${\alpha}-cyclodextrin({\alpha}-CyD)$ and ${\beta}-cyclodextrin({\beta}-CyD)$ with several non-steroidal antiinflammatory drugs were studied on the effects of ${\alpha}-$ and ${\beta}-CyD$ on the solubility of the drugs in aqueous medium. Indoprofen, niflumic acid, alclofenac, and naproxen were chosen as representatives of antiinflammatory drugs. The solubility of all drugs studied increased with the addition of ${\beta}-CyD$, while not with glucose or ${\alpha}-CyD$. The increase of the solubility with ${\beta}-CyD$ was considered due mainly to the formation of inclusion complexes between ${\beta}-CyD$ and drugs. From the solubility data, the apparent stability constants K of the complex could be calculated. Ultraviolet absorption and circular dichroism confirmed the inclusion of indoprofen, niflumic acid and naproxen with ${\beta}-CyD$ in the molar ratio of 1 : 1. Inclusion complexes in solid powder form were obtained by the freeze-drying method and the inclusion formation was confirmed again by infrared, diffential thermal analysis, and X-ray diffraction measurements.

• 대한화학회지
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• 제51권6호
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• pp.526-535
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• 2007

아다만탄 또는 β-cyclodextrin이 결합된 루테늄(II)-터피리딘 착화합물(8, 9, 11)을 합성하고 1H NMR, 13C NMR 및 질량분석스펙트럼으로 구조를 확인하였다. 아다만탄이 결합된 루테늄(II)-터피리딘 착화합물(8, 11) 은 물에 전혀 녹지 않으나, β-cyclodextrin 수용액에 혼합할 경우 β-cyclodextrin과의 초분자를 형성하여 물에 잘 녹아들어갔다. 비슷한 방법으로 수용액 중에서 루테늄(II)-터피리딘 착화합물(8, 11)을 각각 착화합물 9와 혼합 하였을 때, 착화합물(8, 11)의 아다만탄 부분이 루테늄 착화합물 9의 β-cyclodextrin 내부에 포접 됨으로써 안정 한 초분자를 형성하였다.

• KSBB Journal
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• 제11권4호
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• pp.411-419
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• 1996

본 연구에서는, 최근 들어 관심의 대상이 되어 의 품 산업에 응용의 수가 늘고 있는 cyclodextrin을 이용하여 세포밖으로 배출된 식물세포 2차 대사물질을 신속히 포획하여 생산수율을 증대시키고자 하였다. 실험결과 양귀비 현탁배양에서 a-, y-cyclodex trin보다는 ${\beta}$-cyclodextrin이 benzophenan-thridine alkaloid를 생산하는데 더 효과적업을 알았다. 이때 ${\beta}$-cyclodextrin의 optimum concentration이 존재하였는데, 그 농도는 현탁배양배지의 1.5% (w/v) 부 근에서였다. 고체 배지에서 ${\beta}$-cyclodextrin의 첨가는 sangumarme의 생산성을 control의 경우보다 약 4 40배(포함), 약 17배(살포) 정도 증가시켰다. ${\beta}$-cyclodextrin은 현탁배양 배지의 수용액상에서 세포 외로 배출된 alkaloid를 신속히 포획하여 inclusion complex를 형생함으로써 product의 배출을 촉진하여 결과적으로, product pattern을 변화시켜 줌으로 써 생산과 분리가 통시에 이루어질 수 있는 연속공정의 가능성을 확인시켜주었다. 또한 $\beta$cyclodex trin 1.5%(w/v)을 첨가시기를 달리하여 실험한 결과 배양초기에 넣어주는 것이 효과적이었으며, 이때 ${\beta}$-cyclodextrin은 세포성장에 미치는 영향이 없음을 확인하였다. ${\beta}$-cyclodextrin에 의한 benzophenan­t thridine alkaloid의 엄청난 생산 중대 효과는 1) In­c clusion complex 형성에 의한 안정성 증대뿐만 아니라 2) elicitor로서의 역할 등의 복합적 인 요인을 생 각해 볼 수 있는데 2)의 경우에 대한 실험을 세포와 ${\beta}$-cyclodextrin간의 직접적인 접촉을 시키지 않고 해본 결과 미세한 영향을 나타내었으므로 elicitation 의 경우를 완전히 배제한다고 할 수 없었다. 그러나 uv에 의한 inclusion complex의 확인이 1)의 경우를 확인하여 주었으므로 본 실험에서 ${\beta}$-cyclodex­t trin의 역할은 inclusion complex에 의한 생산성 증대라 할 수 있다.