• 생명과학회지
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• 제25권8호
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• pp.910-916
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• 2015

최근 식물 성장에 있어 곰팡이 독소에 관련된 질병에 효과가 있는 Bacillus mojavensis 균주 사용의 보고가 있다. 우리는 B. mojavensis KJS-3균주의 다양한 온도, 염도, 에탄올, pH에서 성장하는 특징을 확인 하였다. B. mojavensis KJS-3균주는 Polymerase chain reaction 분석에 의해 fengycin을 LC-MS/MS 분석을 통해서는 iturin 및 surfactin 와 같은 cyclic lipopeptides를 생산함을 확인 하였다. B. mojavensis KJS-3균주는 식물 유해 곰팡이 균주인 Botrytis cinerea, Rhizoctonia solani AG-4, Sclerotinia sclerotiorum, Colletotricum goeosporioides에 항곰팡이 효과가 있음을 확인하였고 이 결과를 바탕으로 인삼재배에 있어 B. mojavensis KJS-3를 사용하여 성장을 관찰한 결과 뿌리 내에서 성장하여 plant growth promoting endophyte가 있음을 알 수 있었다. 이러한 특징들에 의해 미생물 농약 및 비료로 사용할 수 있을 것이다.

• The Plant Pathology Journal
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• 제34권6호
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• pp.555-566
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• 2018

Two rhizobacteria Bacillus aryabhattai H26-2 and B. siamensis H30-3 were evaluated whether they are involved in stress tolerance against drought and high temperature as well as fungal infections in Chinese cabbage plants. Chinese cabbage seedlings cv. Ryeokgwang (spring cultivar) has shown better growth compared to cv. Buram-3-ho (autumn cultivar) under high temperature conditions in a greenhouse, whilst there was no difference in drought stress tolerance of the two cultivars. In vitro growth of B. aryabhattai H26-2 and B. siamensis H30-3 were differentially regulated under PEG 6000-induced drought stress at different growing temperatures (30, 40 and $50^{\circ}C$). Pretreatment with B. aryabhattai H26-2 and B. siamensis H30-3 enhanced the tolerance of Chinese cabbage seedlings to high temperature, but not to drought stress. It turns out that only B. siamensis H30-3 showed in vitro antifungal activities and in planta crop protection against two fungal pathogens Alternaria brassicicola and Colletotrichum higginsianum causing black spots and anthracnose on Chinese cabbage plants cv. Ryeokgwang, respectively. B. siamensis H30-3 brings several genes involved in production of cyclic lipopeptides in its genome and secreted hydrolytic enzymes like chitinase, protease and cellulase. B. siamensis H30-3 was found to produce siderophore, a high affinity iron-chelating compound. Expressions of BrChi1 and BrGST1 genes were up-regulated in Chinese cabbage leaves by B. siamensis H30-3. These findings suggest that integration of B. aryabhattai H26-2 and B. siamensis H30-3 in Chinese cabbage production system may increase productivity through improved plant growth under high temperature and crop protection against fungal pathogens.