• 제목/요약/키워드: cutting site

검색결과 177건 처리시간 0.021초

합성 기질에 의해 형성된 Lambda Site-specific Recombination 중간 대사물의 분석 (Analysis of Lambda Site-specific Recombination Inermediates Generated by Synthetic Substrates)

  • 이나영;유승구
    • 한국미생물·생명공학회지
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    • 제23권3호
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    • pp.282-287
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    • 1995
  • Integrase (Int) carries out the cutting and resealing of attachment (att) site DNA via a covalent Int-DNA intermediate. A family of synthetic substrate DNAs was designed to accumulate Int-DNA intermediate. Int-DNA intermediates accumulated by half substrate was analyzed by SDS- KCI precipitation and restriction digestion. The results showed that Int-half DNA intermediate was circular and contained covalently bound Int molecule. Int-DNA intermediates were also trapped with three other kinds of synthetic substrates.

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Controlling Mikania micrantha HBK: How effective manual cutting is?

  • Rai, Rajesh Kumar;Sandilya, Madan;Subedi, Rajan
    • Journal of Ecology and Environment
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    • 제35권3호
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    • pp.235-242
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    • 2012
  • Mikania micrantha, a neo-tropical vine, is spreading rapidly in the tropical part of Nepal and is now threatening the rural ecosystem including biodiversity and rural livelihoods. However, no attempt has been made to control the spread of M. micrantha. As a result, the vines are spreading freely and rapidly. After a thorough literature review and assessment of forest management practices, we proposed a manual cutting method, as it suits the Nepalese situation for several reasons: required labor is readily available, as local communities are managing forest patches, and the method does not have any potential adverse effects on non-target native species. Experimental plots were laid out in August 2011 to examine the effectiveness of manual cutting. Two different site types based on canopy coverage were selected and divided into three blocks based on cutting strategy. Four treatments were assigned across the experimental plots following a complete block design. We harvested above-ground biomass according to the assigned treatment. The results suggested that there should be at least two consecutive cuttings within a 3-week interval before flowering, and that three consecutive cuttings resulted in 91% mortality of the vines. In addition, cutting promoted regeneration of native plant species. Employing regular cutting operations can modify understory shade enhancing regeneration of native species, which is a desirable condition to constrain proliferation of M. micrantha. Periodic cuttings reduced the competitiveness of M. micrantha regardless of canopy openness, but native ground cover should be retained.

The Changes of Understory Vegetation by Partial Cutting in a Silvopastoral Practiced Natural Deciduous Stand

  • Kang, Sung Kee;Kim, Ji Hong
    • 한국산림과학회지
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    • 제97권2호
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    • pp.156-164
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    • 2008
  • Recognizing the importance of the multi-purpose management of natural deciduous forest, this study was carried out to implement the partial cutting for stand regulation to examine agroforestry practice as well as other concurrent forest resource production, and to investigate the changes in stand characteristics and understory vegetation in a silvopasture practiced natural deciduous stand in the Research Forest of Kangwon National University, Korea. Three different partial cutting intensities (68.1%, 48.6%, and control) were performed in the unmanaged natural deciduous stand in order to improve the growing condition, especially light condition, for introducing some commercial herbaceous plants on the forest floor to establish agroforestry and/or silvopastoral system. Dominated by Quercus varibilis Blume (50.5%) and Quercus dentata Thum. ex Murray (42.6%), eight tree species were composed of the study forest, including poles of Pinus desiflora Siebold & Zucc and sapling of Pinus Koraiensis Siebold & Zucc. The total of 87 (13 tree species, 12 shrub species, 58 herbaceous species, and 4 woody climbers) vascular plant species were observed in study site after partial cutting treatments, while that of before partial cutting was 53 species (14 tree species, 8 shrubs species, 30 herbaceous species, and 1 woody climbers). The proportion of life form spectra in plot B was Mi (28.4%)-Na (23.0%)-Ge (17.5%)-Ch (10.8%)-He (9.5%)-MM (6.7%)-Th (4.1%). No statistically significant differences were observed in changes of life form spectra from before to after partial cutting treatment and among partial cutting gradients in this study. Partial cutting and scratching for forage sowing made plants invade easily on the forest floor, and light partial cutting (LPC) plot (500 stems/ha) had much higher number of undersory species than those of heavy partial cutting (HPC) plot (310 stems/ha) and control plot (1,270 stems/ha).

할암기를 이용한 무진동 암반 파쇄공법의 현장 적응 사례 (Case History of Vibration-controlled Reckmass Breakage Method by Rock Splitter)

  • 최영천
    • 화약ㆍ발파
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    • 제22권3호
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    • pp.71-78
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    • 2004
  • 국내 재개발 및 건설공사현장에서 암반파쇄 및 굴착 절취 작업의 일환으로 화약을 사용하는 발파공법과 유압 장비를 이용한 무진동 절취 공법, 브레이커를 이용한 파쇄 공법이 대표적으로 사용되고 있다. 유압력을 이용한 무진동 절취공법은 진동, 소음, 비석 등의 공해를 제어하고, 현장에 인접한 보안물건을 보호하며, 기상조건에 제한을 받지 않아 민원을 예방함과 동시에 암 절취 공사의 효율성을 높일 수 있어 암반 파쇄에 따른 여러 분야에서 적용되고 있다. 그러나, 현행 무진동 절취공법은 암반의 특성을 정확하게 파악하지 않고 대부분을 경험에 의존하여 시공패턴을 설정하고, 시공이 행하여지고 있다. 이러한, 각각의 암반 파쇄 공법은 그 적용에 따른 경제적 이익과 시공성, 안전성등을 극대화 할 수 있으나, 반대로 적절하지 못한 공법 적용은 커다란 경제적 손실과 시공성 및 안전성의 저하를 가져오므로 현장 주변 여건과 암반 상황 등 현장 여건을 종합 검토하여 공법 선정이 이루어져야 한다.

Field test and research on shield cutting pile penetrating cement soil single pile composite foundation

  • Ma, Shi-ju;Li, Ming-yu;Guo, Yuan-cheng;Safaei, Babak
    • Geomechanics and Engineering
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    • 제23권6호
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    • pp.513-521
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    • 2020
  • In this paper, due to the need for cutting cement-soil group pile composite foundation under the 7-story masonry structure of Zhenghe District and the shield tunnel of Zhengzhou Metro Line 5, a field test was conducted to directly cut cement-soil single pile composite foundation with diameter Ф=500 mm. Research results showed that the load transfer mechanism of composite foundation was not changed before and after shield tunnel cut the pile, and pile body and the soil between piles was still responsible for overburden load. The construction disturbance of shield cutting pile is a complicated mechanical process. The load carried by the original pile body was affected by the disturbance effect of pile cutting construction. Also, the fraction of the load carried by the original pile body was transferred to the soil between the piles and therefore, the bearing capacity of composite foundation was not decreased. Only the fractions of the load carried by pile and the soil between piles were distributed. On-site monitoring results showed that the settlement of pressure-bearing plates produced during shield cutting stage accounted for about 7% of total settlement. After the completion of pile cutting, the settlements of bearing plates generated by shield machine during residual pile composite foundation stage and shield machine tail were far away from residual pile composite foundation stage which accounted for about 15% and 74% of total settlement, respectively. In order to reduce the impact of shield cutting pile construction on the settlement of upper composite foundation, it was recommended to take measures such as optimization of shield construction parameters, radial grouting reinforcement and "clay shock" grouting within the disturbance range of shield cutting pile construction. Before pile cutting, the pile-soil stress ratio n of composite foundation was 2.437. After the shield cut pile is completed, the soil around the lining structure is gradually consolidated and reshaped, and residual pile composite foundation reaches a new state of force balance. This was because the condensation of grouting layer could increase the resistance of remaining pile end and friction resistance of the side of the pile.

타일 시멘트 전단접착강도와 인장부착강도 비교 및 인발부착강도 타일커팅 영향에 관한 실험적 평가 (Comparison between Shear and Tensile Adhesion Strength of Cement for Ceramic Tiles and an Experimental Evaluation on the Cutting Effect of Tile for Tensile Adhesion Strength)

  • 이상현;기전도;조홈범;김영선;문형재;전현수
    • 한국건축시공학회:학술대회논문집
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    • 한국건축시공학회 2021년도 봄 학술논문 발표대회
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    • pp.181-182
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    • 2021
  • Selecting a proper tile cement for ceramic tile is important to secure tile construction quality. But there is some ambiguity in standards about evaluating adhesion test such as using KS L 1593 in Lab test but using KS L 1592 in site. So, this study aims to how to select proper tile cement for securing adhesion strength in site considering both tensile and shear adhesion strength of cement for ceramic tile. Also, when doing tensile adhesion test in site, there may be adhesion drop because of cutting tile with grinder. As a result, drop of adhesion strength is about 9% ans quality manager consider these factor and should select proper tile cement.

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건축규모에 따른 관리인력의 효율적 배치에 관한 기초연구 -오피스 건물을 중심으로- (A Preliminary Studty on the Efficient Management Manpower Staffing According to the Building Size -Focused on Office Buildings-)

  • 이원석;김신은;김선국;이동훈;원일우
    • 한국건축시공학회:학술대회논문집
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    • 한국건축시공학회 2010년도 춘계 학술논문 발표대회 1부
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    • pp.191-194
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    • 2010
  • Construction companies are now trying a variety of cost-cutting strategies in the midst of recent slump of construction market. Notably, contract workers and local hires are accounting for increasing portion of management manpower as a part of their cost-reduction drive. However, construction site manpower researches to date have focused primarily on productivity analysis of site laborers, with little attention to the productivity of site management resources. This study aims to analyze the staffing status of office building construction site management manpower in reference to project size. Conclusions herein will provide basic inputs for subsequent attempts to build a model to enable efficient staffing of construction site management manpower.

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CNC를 활용한 가구디자인 표현 기법 연구 (A Study of Expression Technique in Furniture Design using CNC Machine)

  • 김건수;이상일;이성용
    • 한국가구학회지
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    • 제25권1호
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    • pp.45-54
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    • 2014
  • Wood cutting utilizing CNC art in the field of furniture design industry, art, education are diverse. However, there is lack of data in the case for wood cutting, such as cutting conditions and wood cutting. So this article is to establish furniture design processing using CNC. The researchers investigated the processed products using CNC, the data of the web site and CNC relevant articles, then organized its impact on the furniture industry today. History and definition of CNC have studied for a discussion of the advantages and disadvantages. Then, the researchers analyzed the cases to investigate the wood cutting conditions, was applied to the fabrication of furniture domestic and foreign. CNC organized systematically design information through the computer So, it allowed to reduce the repetitive behavior that has to work hand in the furniture manufacture existing. CNC has made it possible to design a fine complex in furniture design industry. So it became possible to make a new representation and production of various forms. Material about CNC are mostly for milling machine and shelves for metal processing. So, the researchers investigated and precautions general content of wood cutting. The enhanced understanding to investigate an example that is applied outside the country, is used to analyze the expression various techniques CNC. It is difficult to obtain accurate data processing, it has various characteristics in the same timber, Future research is about analyzing type applications and CNC machining range at the time of processing the various wood.

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파일 두부정리 자동화 장비를 위한 두부정리선 센싱 방법 (Cutting-Line Sensing Methods for an Automated Concrete Pile Cutter)

  • 김성근;김영석;이준복
    • 대한토목학회논문집
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    • 제26권6D호
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    • pp.985-993
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    • 2006
  • 건설현장에서 기성 콘크리트 파일의 사용이 점차적으로 증가하고 있다. 파일기초를 형성하는 주요 작업들 중의 하나가 콘크리트 파일의 두부를 $800kg/cm^2$ 이상의 압력으로 파쇄하는 것이다. 일반적으로 파일 두부정리 작업은 크러셔와 3~4명의 숙련공에 의하여 실시된다. 최근 파일 두부정리에 관한 보고에 의하면 파일의 강도를 상당히 감소시키는 균열이 작업 중에 많이 발생하고 있고 파일 두부정리 작업이 매우 반복적이고 노동집약적인 것으로 나타났다. 기존의 파일 두부정리 작업의 생산성, 안전성, 품질을 향상시키기 위하여 두부정리 자동화 장비를 개발하기 위한 연구가 진행되고 있다. 본 논문에서는 두부정리선을 인식하기 위한 센싱방법과 작동 프로세스 알고리즘을 제시하고 있다. 이것은 두부정리 자동화 장비를 개발하기 위하여 매우 중요하다. 제안된 센싱방법 중 1가지를 적용한 파일롯 타입의 두부정리 자동화 장비를 개발하였으며, 현장에서 기능을 실험하였다.

부산 신항만 욕망산 경관계획 (Mt. Yokmang Landscape Planning in Busan New Port)

  • 김충식
    • 한국조경학회지
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    • 제33권6호
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    • pp.127-139
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    • 2006
  • Mt. Yokmang landscape design awarded first in [North container terminal site furtherance construction in Busan new port], design competition sponsoring in the Korea Container Terminal Authority in 2005. Design guideline was to propose the symbol of new port and to establish landscape plan in consideration of view and geological location. Because landscape plan required establishment of restoration plan for cutting slope, Mt. Yokmang became the major object. The purpose of planning are as follows. The first purpose is to minimize existent natural environment damage, and the second, to connect with ambit and create new image. The third is to provide place of rest and interchange for local resident. Reflecting stratum structure and landscape characteristics, cutting slope consisted of [utilization slope], [presentation slope], [landscape slope], and [ecological slope]. Vegetation design applied ecological restoration method through restoration of stratum, and eve environment-friendly afforestation, planned program that can connect area with existing ecosystem. In process that decides form of the cutting slope, analyze view structure and visual exposure in various access routes, sensitivity etc. was accomplished. Also, symbolic tower(Ocean Polaris) that presents in architecture and landscaping features, night landscape planning could gain synergy effect by keeping consistence with landscape and ecological planning. Passing through final design and construction process, I expect that the Mt. Yokmang will be a new landmark in Busan new port.