• 대한수의학회지
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• 제31권4호
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• pp.491-500
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• 1991

This study was conducted to detect the serum antibodies in the experimentally toxoplasma infected dogs and street dogs by use the of an enzyme-linked immunosorbent assay (ELISA). And this test was performed on the polystylene microplate by coating with the tachyzoites soluble antigen of T gondii (RH strain), incubated with sera diluted then, added with HPO-conjugated rabbit anti-dog IgG and o-phenylenediamine used as a substrate. Tachyzoites of T gondii harvested from mouse peritoneal cavity were purified by 30, 40 and 50% Percoll density gradient centrifugation and used as the source of antigen. The results obtained were summarized as follows; 1. The highest ratio of positive to negative (P/N ratio) was obtained at the level of $l{\mu}g/ml$ protein concentration of antigen with the 1/4000 dilution of the conjugate measured by checker-board titration. It was regarded as the optimum concentration of the antigen and conjugate. 2. Cut-off value in this IgG ELISA was 0.375 that was determined by mean absorbance (at 492nm) of IFA negative serum added with the dauble value of the standard deviation $(mean{\pm}2S.D.)$. 3. Serum ELISA IgG antibodies to T gondii in the exyerimentally infected dogs were detected firstly at the Week 3 after inoculation and the highest titer was recognized at the Week 4, 5 and 6 after inoculation. 4. Stability of the antigen absorbed in the microplates that were preserved at $4^{\circ}C$ and $-25^{\circ}C$ separately were prolonged up to 3 weeks and 10 weeks at $4^{\circ}C$ and $-25^{\circ}C$, respectively. However the reproducibility was not reliable after the preservation of 4 weeks and longer. 5. Positive rate of the specific antibodies in 312 test sera was 28.5% and there was no significant differences between the male (27.8%) and female (29.5%), respectively. 6. The IgG ELISA was proved to be a specific procedure for the detection of antibodies to canine toxoplasma infection and also evaluated as a screening test for the large scale of test samples in laboratory.

• 농촌의학ㆍ지역보건
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• 제16권1호
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• pp.79-89
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• 1991

A systematic study was conducted to identify and isolate a serologically pertinent antigen with high specific activity and low cross reactivity from Cysticercus parenchymal antigen. Differential centrifugation of the homogenate yield three particulate and one soluble fractions ; the $480{\times}G$ pellets($CyL_2$), the $7650{\times}G$ pellet($CyL_3$), the $100000{\times}G$ pellet($CyL_4$), and $100000{\times}G$ supernatant($CyL_6$). We compared antigenicity of these antigens to that or cystic fluid antigens($CyF_1$), saline extract of cystic wall($CyL_1$), and n-butanol treated $GyL_4$ antigen ($CyL_6$) based on SDS-PAGE and immunoblot techniques. The data obtained were as follows : 1) The ratio of O.D. value of ELISA against cysticercosis positive pool sera to that of negative pool sera was highest when using $CyF_1$ as antigen. However the ratio was relatively low in case of $CyL_{3.4}$ and $CyL_5$. 2) We have noted in previous paper that most strong antigenic activities are present in 63Kd band with low cross reactivities. An effective serologic reagent must contain components that are recognized by most infected sera. 63Kd band met this criteria and could be considered as a reliable band for the diagnosis of cysticercosis. As far as 63Kd band concern, $CyL_5$ showed most strong activities without disturbance of cross reaction by EITB in spite of low applicability to microplate ELISA. 3) $CyL_5$ could detect the serum antibody of cysticercosis even in very low titers, around cut-off values of microplate ELISA, by immunoblot. It also could detect the cross reactivities of Echinococcus species, which showed high absorbance value in micro plate ELISA and some sparganosis cases. Further purification of this antigen will be able to represents a antigen that can be used in the diagnosis of cysticercosis.

• 대한전자공학회논문지TC
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• 제41권8호
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• pp.105-111
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• 2004

본 논문에서는 밀리미터파 대역에서 고이득과 광대역 특성을 갖는 MHEMT(Metamorphic High Electron Mobility Transistor) cascode 증폭기를 설계 및 제작하였다. Cascode 증폭기 제작을 위해 0.1 ㎛ InGaAs/InAlAs/GaAs MHEMT를 설계ㆍ제작하였다. 제작된 MHEMT는 드레인 전류 밀도 640 mA/mm, 최대 전달컨덕턴스(gm)는 653 mS/mm를 얻었으며, 주파수 특성으로 f/sub T/는 173 GHz, f/sub max/는 271 GHz의 우수한 특성을 나타내었다. Cascode 증폭기는 CPW 전송선로를 이용하여 광대역 특성을 얻을 수 있도록 정합회로를 설계하였으며, 1단과 2단 증폭기의 2가지 종류로 회로를 설계하였다. 설계된 증폭기는 본 연구에서 개발된 MHEMT MIMIC 공정을 이용해 제작되었다. 제작된 cascode 증폭기의 측정결과, 1단 증폭기는 3 dB 대역폭이 31.3∼68.3 GHz로 37 GHz의 넓은 대역 특성을 얻었으며, 대역내에서 평균 9.7 dB 및 40 GHz에서 최대 11.3 dB의 S21 이득 특성을 나타내었다. Cascode 2단 증폭기는, 3 dB 대역폭이 32.5∼62.0 GHz로 29.5 GHz의 대역폭과 대역내에서 평균 20.4 dB 및 36.5 GHz에서 최대 22.3 dB의 높은 이득 특성을 얻었다.

• 전자공학회논문지
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• 제53권5호
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• pp.152-159
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• 2016

본 논문은 CMOS 자기센서(hall Sensor)의 오프셋 및 1/f 잡음 제거기술 기반 고선형 자기센서 신호처리장치를 제안한다. 제안하는 자기센서는 자계(magnetic Field)를 감지하여 자계의 변화량에 따른 홀 전압(hall Voltage)을 출력하는 홀 플레이트(hall Plate)와 홀 플레이트 출력 신호의 오프셋과 1/f 잡음 제거 및 디지털화를 위한 자기센서 신호처리시스템으로 구성된다. 자기센서 신호처리 시스템은 스피닝 전류 바이어싱(spinning current biasing)을 통해 자기신호로부터 오프셋과 1/f잡음 성분을 분리하고, 초퍼 및 증폭기를 통해 자기신호를 100 kHz 주파수 대역으로 변조한다. 60 kHz 차단주파수를 갖는 고역통과필터(highpass filter)를 사용하여 오프셋 및 1/f 잡음을 제거한뒤 ADC(analog to digital converter)를 통해 자기신호만을 디지털 변조한다. 증폭기 및 고역통과필터 출력은 자기신호 -53.9 dBm @ 100 kHz, 잡음성부은 -101.3 dBm @ 10 kHz이다. 최종적으로 ADC를 통과한 자기센서 출력은 -5.0 dBm @ 100 kHz이고, 오프셋 및 1/f 잡음은 -55.0 dBm @ 10 kHz이다.

• 한국전자파학회논문지
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• 제18권8호
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• pp.887-894
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• 2007

본 논문에서는 전기 결합 구조와 추가된 개방 스터브를 이용해 제이 가능한 4개의 전송 영점을 갖는 저역 통과 필터를 설계 및 제작하였다. 필터의 통과 대역은 GSM 대역이며, 전기 결합에 의해 WiBro 및 위성 DMB 대역에서 전송 영점이 각각 발생한다. 또한, 임의의 상측 주파수에서 개방 스터브에 의해 추가적인 2개의 전송 영점이 발생하는 구조이다. 적은 기생 성분을 갖는 준집중 소자를 이용해 필터를 구현함으로써 차단 대역의 고조파 성분을 억제하였다. 유한 전송 영점의 제어를 위한 등가 회로의 전기 결합 성분인 $C_M$은 필터 구조의 개방 스터브 간 간격 조절을 통해 구현하였다. 유전율 2.6인 테프론 기판을 사용하여 제작된 필터의 전체 크기는 급전선로를 포함하여 $38{\times}20{\times}0.79mm^3$이다. 측정된 3dB 차단 주파수는 1.55GHz이며, 전송 영점의 위치는 각각 2.20, 2.43, 4.11 및 6.84 GHz이다.

• 한국환경보건학회지
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• 제40권6호
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• pp.492-501
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• 2014

Objectives: The purpose of this study was to assess the validity of self-reported cigarette smoking status and investigate factors associated with the accuracy self-reported and measured urinary cotinine in Korean adults. Methods: We used data from the $1^{st}$ Korean National Environmental Health Survey (2009-2011) among adults aged ${\geq}19$ years (N=6,246). The survey examined self-reported smoking status, and urinary cotinine was regarded as the biomarker of exposure to tobacco smoke. Urinary cotinine was analyzed using a gas chromatography-mass spectrometry (GC/MS) and data analysis was conducted using IBM SPSS version 20.0, which uses the sample weight and calculates variance estimates to adjust for the unequal probability of selection into the survey. Results: We calculated a cut-off point (53.3 ug/L) by using a ROC (Receiver Operating Characteristic) curve. The smoking prevalence was 24.6% based on self-reported data and 28.2% based on urinary cotinine concentrations. When we assessed the agreement between self-reported and urinary cotinine, we found an average agreement of 97.7% among self-reported smokers and 94.5% among self-reported non-smokers. Among self-reported smokers, factors affected the discrepancy were age, household economic status and average number of cigarettes smoked per day. On the other hand, gender, former smoking experience, and exposure to SHS (second hand smoke) were associated with discrepancies among self-reported non-smokers. Conclusion: These results suggest that self-reported data on smoking status provide a valid estimate of actual smoking status. In future research, we will conduct a continuous monitoring study for reliability verification of the data to reduce potential interpretation errors.

• 한국지반공학회논문집
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• 제36권10호
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• pp.21-31
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• 2020

본 연구에서는, 제주도 현무암 암석의 삼축압축시험 결과에 대한 비선형 회귀분석을 통하여 Hoek-Brown 계수(m_i)를 계산하였다. 그리고 제주도 현무암 암석의 일축압축강도(UCS), 압열인장강도(BTS) 및 압열인장강도에 대한 일축압축강도의 비(UCS/BTS)와 m_i의 관계를 각각 살펴보았으며, 제주도 현무암의 Hoek-Brown 파괴 포락선을 결정하는데 있어서, 인장 및 압축파괴영역에서 이용할 수 있는 방법을 제안하였다. 그 결과, 제주도 현무암 암석의 UCS 및 BTS와 m_i 사이에는 뚜렷한 상관관계가 없었으며, UCS와 m_i/UCS 및 BTS와 m_i/BTS 사이에는 멱함수와 지수함수의 상관관계가 있음을 알 수 있었다. 제주도 현무암의 UCS/BTS와 m_i 사이에는 뚜렷한 상관관계가 없었으며, Hoek-Brown 파괴기준에 의해 계산되는 인장강도는 제주도 현무암의 인장강도를 과소평가하고 있다는 것을 알 수 있었다. 본 연구에서 제시한 방법은, 제주도 현무암의 인장 및 압축파괴영역에 대한 Hoek-Brown 파괴 포락선을 결정하는데 있어서 유용하게 이용할 수 있을 것이라 사료된다.

• Asian Pacific Journal of Cancer Prevention
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• 제13권7호
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• pp.3281-3285
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• 2012

Objectives: To evaluate the clinicopathologic correlation and prognostic value of HPV18 DNA viral load in patients with early-stage cervical neuroendocrine carcinoma (NECA). Methods: Formalin-fixed, paraffin-embedded tissue of cervical NECA patients with known HPV18 infection and clinicopathologic data including follow-up results were collected. The HPV18 DNA load was assessed with quantitative PCR targeting the HPV18 E6E7 region. Results: Twenty-one patients with early-stage (IB-IIA) cervical NECA were identified. HPV18 DNA viral load ranged from 0.83 to 55,174 copies/cell (median 5.90). Disease progression, observed in 10 cases (48%), was not significantly associated with any clinicopathologic variables. However, the group of patients with progressive disease tended to have a higher rate of pelvic lymph node metastasis (50% versus 9%, p=0.063) and a lower median value of HPV18 DNA viral load (4.37 versus 8.17 copies/cell, p=0.198) compared to the non-recurrent group. When stratified by a cut-off viral load value of 5.00 copies/cell, the group of patients with viral load ${\leq}5.00$ copies/cell had a significantly shorter disease-free survival than the group with viral load >5.00 copies/cell (p=0.028). The group with a lower viral load also tended to have a higher rate of disease progression (75% versus 31%, p=0.080). No significant difference in the other clinicopathologic variables between the lower and higher viral load groups was identified. Conclusion: HPV18 DNA viral load may have a prognostic value in patients with early-stage NECA of the cervix. A low viral load may be predictive of shortened disease-free survival in these patients.

• Asian Pacific Journal of Cancer Prevention
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• 제17권12호
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• pp.5101-5106
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• 2016

Purpose: We aimed to establish an inflammatory prognostic index (IPI) in early and advanced non-small cell lung cancer (NSCLC) patients based on hematologic and biochemical parameters and to analyze its predictive value for NSCLC survival. Materials and Methods: A retrospective review of 685 patients with early and advanced NSCLC diagnosed between 2009 and 2014 was conducted with collection of clinical, and laboratory data. The IPI was calculated as C-reactive protein ${\times}$ NLR (neutrophil/ lymphocyte ratio)/serum albumin. Univariate and multivariate analyses were performed to assess the prognostic value of relevant factors. Results: The optimal cut-off value of IPI for overall survival (OS) stratification was determined to be 15. Totals of 334 (48.8%) and 351 (51.2%) patients were assigned to high and low IPI groups, respectively. Compared with low IPI, high IPI was associated with older age, greater tumor size, high lymph node involvement, distant metastases, advanced stage and poor performance status. Median OS was worse in the high IPI group (low vs high, 8.0 vs 34.0 months; HR, 3.5; p<0.001). Progression free survival values of the patients who had high vs low IPI were determined 6 months (95% CI:5.3-6.6) and 14 months (95% CI:12.1-15.8), respectively (HR; 2.4, P<0.001). On multivariate analysis, stage, performance status, lactate dehydrogenase and IPI were independent prognostic factors for OS. Subgroup analysis showed IPI was generally a significant prognostic factor in all clinical variables. Conclusion: The described IPI may be an inexpensive, easily accessible and independent prognostic index for NSCLC patients, useful for clinical practice.

• 복식
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• 제4권
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• pp.13-24
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• 1981

The color yellow was considered from ancient time to the Chosun Dynasty as the central color. Thus, this color became the royal color for the costumes in the palace. It is generally known to usthat the color yellow was controled in use both for the general public and in the royal palaces. However, in the later part of Chosun Dynasty, the color yellow was used not only for the king's costumes but also used for women's tops and for the linings of clothing. Especially, in some of the costumes that belonged to the later period of the Chosun Dynasty, we can still see lots of bright yellow tops. Also there are many green dyed official robes and various costumes for women. It is a true fact that people could not derive the color green from the plants as they did with yellow. The only way they could make the color green was to mix indigo and yellow together. By repeating the difficult process of making various dyes constantly during many centuries, the Korean people developed the marvelous technique of making natural color. Those plants used to make the color yellow are ; Gardenia, Phellodendron amurense, Turmeric, Coptis, Safflower, Arthraxon hispidus, Styphnolobium japonicum. While synthetic dye causes pollution, natural coloring by plants is as safe and useful as the color itself is lovely. Yet it is tragic to know that this traditional culture of making beautiful natural colors was cut off. There is no way to know today the traditionally correct method to derive colors from the plants. Therefore, it is our aim and challenge to find out the original way to dye and develop it and preserve it as our non-polluted folk art. In regard to natural dyeing, we must say that is very difficult to prepare and preserve natural dyes. In the first place, people had to get the right plants at an appropriate time. Then they could not keep those plants too long. Finally, much depended upon the mordant as well as various conditions and dyeing procedures. All those things influenced greatly the quality of color, some times producing a very pretty color and other times a very dull one. It is very appropriate that the natural dye art should be recognized and appreciated anew by Korea since it provides satisfaction to historical and folk artistic demands as well as to those of fashion conscious modern society for high quality consumption items. We propose two stages of development. The first stage is to explore native dye plants and encourage their cultivation. The second stage is to extract from the plants desirable dye which will enhance national culture.