The present study was conducted to observe the effect of osmolality of glycerolated TEST-yolk glycerol extenders on post-thawing sperm kinematics of ram spermatozoa of the native Malpura breed maintained in a semi-arid tropical environment. Good quality semen obtained from adult rams was pooled, split and diluted to 1,000 million spermatozoa per ml in complete TEST-yolk-glycerol extenders of 900, 1,200, 1,500 and 1,800 mOsm/kg osmolality. Diluted semen samples were loaded in 0.25 ml straws and cooled down to $-125^{\circ}C$ freezing temperature at the rate of $-25^{\circ}C$ per minute under controlled conditions before plunging into liquid nitrogen for storage. The thawing of straws was performed at $50^{\circ}C$ in a water bath for 10 seconds and sperm kinematics of the frozen-thawed spermatozoa were assessed by a computer-assisted sperm analysis technique. Osmolality of diluent had no significant effect on post-thawing % motility, % rapid, % medium and % slow moving frozen-thawed spermatozoa but significantly (p< 0.05) affected the % linearity and % straightness. The post-thawing % motility and % rapid motile spermatozoa were highest in samples extended in diluent of 1,500 mOsm/kg osmolality and lowest in 900 mOsm/kg. The curvilinear velocity of spermatozoa was significantly (p<0.05) higher for samples extended in 1,800 mOsm/kg, compared to those in 900 and 1,200 mOsm/kg, but the effect was not significantly different to those extended in diluent of 1,500 mOsm/kg osmolality. The study indicated that ram spermatozoa could tolerate a wide osmolality range for dilution in the complete TEST-yolk-glycerol extender for their cryosurvival. The highest recovery of motile spermatozoa following thawing was achieved in samples extended in the TEST-yolk-glycerol diluent of 1,500 mOsm/kg osmolality.
Purpose: To correct the facial asymmetry and to achieve symmetry and balance, not only the soft tissue restoration of deficits but also creation and facial contour line such as mandible border and angle is important. Micro fat graft has limitation such as high resorption rate and somewhat limited ability to emphasize the rigid bony characteristics of the mandible angle due to its innate soft consistency. We have investigated the advantages of dermal fat graft over micro fat graft to correct asymmetry of the lower face in patients who had undergone mandibular reconstruction or distraction, using comparative analysis. Methods: Total of 12 patients were enrolled in our study: 6 micro fat graft and 6 dermal fat graft. Postoperative results were compared and analyzed at immediate postoperative period and more than 1 year later in each group with photographs, and analysised with image J program. Result: No complications were noted both in the micro fat type and the dermal fat type of procedures such as fat necrosis or micro calcifications. All of the patients who received micro fat graft, however had considerable amount of fat resorption after the procedure which led to two additional fat graft procedures. Although minor contour obliteration due to contracture was seen in patients who had undergone dermal fat graft procedure, no definite resorption was found even after more than one year follow-up. Results of dermal fat graft patients were satisfactory in terms of mandible angle symmetry. Secondary revision was necessary in one case due to overcorrection using dermal fat graft. Conclusion: The dermal fat graft has many advantages over the conventionally more popular micro fat graft to correct asymmetry of the lower face following mandible reconstruction owing to its lower resoption rate, more effective in emphasizing the natural curvilinear anatomical contours of the mandible angle and body and lower complication rates such as fat necrosis or micro calcifications.
An eddy-resolving free-surface primitive-equation model with nonlinear terrain-following coordinates is established to study the exchange of water masses among the Asian marginal seas and their adjacent waters. A curvilinear coordinate system is used to generate the horizontal grid with a variable resolution for the regional oceans from $5^{\circ}$S to $45^{\circ}$N and $100^{\circ}$E to $155^{\circ}$E. The higher resolution region has about a 10 km by 10 km grid covering the complex geometry of the coastal marginal seas, while the lower resolution region has about a 30 km by 30 km grid covering the eastern Pacific. The model is initialized by the Levitus annual climitology and forced by the monthly mean air-sea fluxes of momentum, heat, and freshwater derived from the Comprehensive Ocean-Atmosphere Data Set. High-resolution and low-viscosity are identified as the key factors for a better representation of the exchange of waters through narrow straits and passages between the marginal seas and their adjacent waters. The dynamics of the loop currents and eddies in the South China Sea and Celebes Sea are examined in detail. It has found that the anticyclonic loop and detached eddies from the Kuroshio through the Luzon Strait play an important role in transporting warm and salty water into the South China Sea, while the cyclonic circulation of the Mindanao Current in the Celebes Sea plays a role in contributing cold water to the Indonesian throughflow. The deep undercurrent of the western Pacific is shown to provide fresher water to the South China Sea and Celebes Sea. These modeling results suggest that the exchange processes via the narrow straits and passages are of fundamental importance to the maintenance of water masses for the marginal sea region.
Even the same materials are assembled in flush door skin panel, warping is not simply prevented under the changes of environmental conditions since wood and wood-based material have large variations in their physical and mechanical properties. The parameters such as linear movement coefficient(LMC), modulus of elasticity (MOE), required to predict warping could be estimated by oven drying method and dynamic method instead of American Society for Testing Materials(ASTM) procedure. The relationship between warping and LMC was curvilinear, while it between warping and MOE was linear. LMC had a larger effect on warping than MOE. Material propensity of skin panel such as hardboard and plywood showed normal distributions. The variation of material properties, however, was much larger in plywood than in hardboard. Monte Carlo simulation also indicated that rejection ratio of flush door due to the occurrence of warping could be predicted with consideration of the relationship of warping and parameters of probability distribution of MOE, LMC, and moisture content.
These experiments were conducted to examine the effects of theophylline, pentoxifylline and heparin on frozen-thawed Hanwoo sperm for enhancing motility and viability of sperm. Frozen-thawed semen collected from one bull was treated in TALP(tyrode-albuminlactate-pyruvate) containing varous concentrations of theophylline and pentoxifylline. After incubated at 5% CO2 in air atmosphere for 6 hours, the motility of sperm after the treatments was characterized by CASA(computer aided semen analysis) system. When monitored notility(MOT) and curvilinear velocity(VCL), theophylline and pentoxifylline exerted their optimal action at the concentration of 30 mM and 3 mM, respectively. No difference of sperm motility was observed when the sperm was treated with both substances compared with a single treatment of each substance. Comparison was then made for evaluating the effect of theophylline and / or pentoxiophylline on the motility and viability of significant treatment effects of each substance, high MOT and VCL values were detected in sperm treated with theophylline. In the case of sperm viability examined by an eosin-nigrosin staining, however, a significant decrease was found after the combined treatment of theophylline+pentoxyphilline than after the treatment with heparin alone or no treatment(P<0.05). In conclusion, theophylline, pentoxiphylline or heparin can be used for enhancing the motional characteristics and viability of frozen thawed Hanwoo semen. Considering characteristics of these substances, theophyline may be useful in the artificial insemination system, which requires vigorous sperm motility. While, heparin supporting sperm viability in vitro can be effectively used for improving in vitro-fertilization system.
Purpose: Reconstruction of small defects of the dorsal fingers and toes is a challenging task. Although adipofascial flap is widely used for these areas, additional refinements are warranted. In this paper, we define the appropriate defect size in the finger and toes that can be treated with the adipofascial flap, refine its surgical indications and present a few surgical tips. Materials and Methods: Twelve patients with dorsal defects of the fingers and toes were treated with a random-type adipofascial turn-over flap and skin graft. If the defect area exceeded the size that could be covered by a conventional design, the flap base was designed in oblique or curvilinear fashion to lengthen the flap. For accurate defect coverage, the width of the flap base was designed in an asymmetrical shape depending on the defect configuration, varying the width from 0.3 to 1.0 cm, as opposed to the standard 0.5 to 1.0 cm width. Moreover, the lateral limit of the flap was defined as the lateral axial line. The size of the defect ranged from $3.0{\times}1.7cm$ to $1.5{\times}1.3cm$. Results: All flaps survived completely. Gliding function of the hand was well preserved and there was no evidence of tendon adhesion. Conclusion: The small defect in the dorsal finger and toe can be defined as less than one phalanx-length, measuring about $3.0{\times}2.0cm$ in size. If the defect exceeds this dimension, it is recommended that a different option be considered. We believe the adipofascial flap is an excellent option for treating small defects.
Magazine of the Korean Society of Agricultural Engineers
/
v.30
no.4
/
pp.134-153
/
1988
This paper was carried out to investigate the existence of a unique stress- strain behavior by obtaining some factors influencing the time dependent stress- strain behavior of clay. The results obtained from this study were summarized as follows ; 1. The relationship between stress ratro and strain in normally consolidated clay was in- dependent on pre-shear consolidation pressure. Therefore, shear strain could be expressed as a function with stress ratio. 2. The constitutive equation of shear strain on Modified Carn Clay Model coincided better with the observed value than Cam Clay Model. 3. The relationships between deviator stress and shear strain, between pore water pressure and shear strain were unified by the mean equivalent pressure. 4. The shear strain contour in norrnally consolidated clay was increased linearly through origin, but that in overconsolidated clay was not in accordance with the result of the former. 5. Because the effective stress path of normally consolidated clay was unified by the mean equivalent pressure, state boundary surface in (e,p,q) space was transformed into two dimensional surface. But it was considered to be suitable that the unified stress- strain in overconsolidated clay be expressed by a function with overconsolidation ratio. 6. The deviator for constant strain was increased linearly with increment of strain rate ($\varepsilon$) on semi-log scale, but pore water pressure was decreased. 7. The behavior of stress relaxation was transformed from linear to curvilinear with inc - rement of strain rate before stress relaxation test, and pore water pressure was increased in total range. 8. The strain of creep was increased linearly with increment of time on semi-log scale. The greater the strain rate before creep test became, the greater the increment of strain of creep became. And the pore water pressure during creep test was increased generally with increment of time on semi-log scale.
Magazine of the Korean Society of Agricultural Engineers
/
v.40
no.6
/
pp.70-78
/
1998
The changes of tidal characteristics of Kyonggi-Bay Region after the Large Reclamations in the Inchon Coastal Area have been studied by the numerical modelling. The constructions of Inchon International Airport and Sihwa Seadike were considered as the main reclaimation projects in this region. A two dimensional hydrodynamic model was used for this study, and it was composed in relatively high resolution with curvilinear orthogonal coordinate. As the results, at the Inchon harbor and Palmido, where a deep channel is developed, the semi tidal ranges(Z$_0$) were decreased in 8 cm after the two reclamations. However, the Z$_0$ values in the outside of the deep channel were decreased only in 2~4 cm, so that the tidal changes in the deep channel were greater than in the outside of it. After the reclamations, the maxmum velocity was increased 17% at the Inchon harbor, however it was decreased from 1. 55 m/s to 1.10 m/s at the Palmido. This is because the available area in which the stream may spread out was largely reduced after the reclamations. An Asan-bay region, the Z$_0$ was decreased in 5 cm at the Punyang-man and Asan-man stations located after the reclamations in Inchon coastal area. However the changes of Z$_0$ were very little at the outside of Asan-bay. In Asan-bay, the current velocity and direction were little influenced by the reclamations in Inchon coastal area.
Journal of the Computational Structural Engineering Institute of Korea
/
v.31
no.2
/
pp.71-78
/
2018
This paper presents the assembling of multiple patches based on the single patch isogeometric formulation for the shear deformable shell element given in the previous study. The geometrically exact shell formulation has been accomplished with the shell theory based formulation and the generalized curvilinear coordinate system directly derived from the given NURBS geometry. For the knot elements matching across adjacent surfaces, the zero-th and first parametric continuity conditions are considered and the corresponding coupling constraints are implemented by a master-slave formulation between adjacent patches. The constraints are then enforced by a substitution method for condensation of the slave variables, thereby reducing the model size. Through numerical investigations, the important features of the first parametric continuity condition are confirmed. The performance of the multi-patch shell models is also examined comparing the rate of convergence of response coefficients for the zero and first order continuity conditions and continuity in coupling boundary between two patches is confirmed.
We reported previously that the administration of Korean red ginseng water extract (KRG-WE) protected the guinea pig testis against damage induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (a potent endocrine disruptor). We also found that crude saponin from ginseng was the active ingredient responsible for this protection. Here, we examined the biological role of KRG-WE in an animal model of age-induced dysfunction of spermatogenesis. Twenty-four male Sprague-Dawley (six 2-month-old and eighteen 12-month-old) rats were used. The young and old control groups received only vehicle. The ginseng saponin (GS)- and KRG-WE-treated groups received GS (40 mg/kg body weight/day) and KRG-WE (200 mg/kg body weight/day), respectively, for 4 months. The number of cells, Sertoli cell index, Johnsen's score, and sex hormone levels decreased significantly with age. However, the administration of KRG-WE and GS markedly improved the number of germ cells, seminiferous tubular size, and Johnsen's score in the old rats. Ginseng produced a distinct testicular histological improvement in old rats. KRG-WE and GS elevated testosterone levels, while attenuating the aberrant increase in follicle stimulating hormone and luteinizing hormone levels. Sperm kinematics evaluated by a computer-assisted sperm analyzer demonstrated improvement in the percentage of motile sperm, progressive sperm motility, and curvilinear velocity associated with sperm quality, supporting the beneficial role of red ginseng in senile spermatogenesis. Overall, the total water extract had a more potent effect than the corresponding saponin fraction. In conclusion, Korean red ginseng rejuvenated age-induced testicular dysfunction. Additionally, the total water extract was more potent than the corresponding saponin fraction.
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