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http://dx.doi.org/10.5142/jgr.2010.34.4.327

Panax ginseng Improves Senile Testicular Function in Rats  

Hwang, Seock-Yeon (Department of Clinical Pathology, Daejeon University)
Sohn, Sang-Hyun (Department of Life Science, College of Biomedical and Health Science, Konkuk University)
Wee, Jae-Joon (Korea Ginseng Corporation)
Yang, Jin-Bae (Department of Clinical Pathology, Daejeon University)
Kyung, Jong-Soo (Korea Ginseng Corporation)
Kwak, Yi-Seong (Korea Ginseng Corporation)
Kim, Sung-Won (Department of Life Science, College of Biomedical and Health Science, Konkuk University)
Kim, Si-Kwan (Department of Life Science, College of Biomedical and Health Science, Konkuk University)
Publication Information
Journal of Ginseng Research / v.34, no.4, 2010 , pp. 327-335 More about this Journal
Abstract
We reported previously that the administration of Korean red ginseng water extract (KRG-WE) protected the guinea pig testis against damage induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (a potent endocrine disruptor). We also found that crude saponin from ginseng was the active ingredient responsible for this protection. Here, we examined the biological role of KRG-WE in an animal model of age-induced dysfunction of spermatogenesis. Twenty-four male Sprague-Dawley (six 2-month-old and eighteen 12-month-old) rats were used. The young and old control groups received only vehicle. The ginseng saponin (GS)- and KRG-WE-treated groups received GS (40 mg/kg body weight/day) and KRG-WE (200 mg/kg body weight/day), respectively, for 4 months. The number of cells, Sertoli cell index, Johnsen's score, and sex hormone levels decreased significantly with age. However, the administration of KRG-WE and GS markedly improved the number of germ cells, seminiferous tubular size, and Johnsen's score in the old rats. Ginseng produced a distinct testicular histological improvement in old rats. KRG-WE and GS elevated testosterone levels, while attenuating the aberrant increase in follicle stimulating hormone and luteinizing hormone levels. Sperm kinematics evaluated by a computer-assisted sperm analyzer demonstrated improvement in the percentage of motile sperm, progressive sperm motility, and curvilinear velocity associated with sperm quality, supporting the beneficial role of red ginseng in senile spermatogenesis. Overall, the total water extract had a more potent effect than the corresponding saponin fraction. In conclusion, Korean red ginseng rejuvenated age-induced testicular dysfunction. Additionally, the total water extract was more potent than the corresponding saponin fraction.
Keywords
Red ginseng; Crude saponin; Sperm quality; Spermatogenesis; Aging;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 4  (Related Records In Web of Science)
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1 Mulhall JP, Daller M, Traish AM, Gupta S, Park K, Salimpour P, Payton TR, Krane RJ, Goldstein I. Intracavernosal forskolin: role in management of vasculogenic impotence resistant to standard 3-agent pharmacotherapy. J Urol 1997;158:1752-1758.   DOI   ScienceOn
2 Chen X, Lee TJ. Ginsenosides-induced nitric oxide-mediated relaxation of the rabbit corpus cavernosum. Br J Pharmacol 1995;115:15-18.   DOI   ScienceOn
3 Choi HK, Seong DH, Rha KH. Clinical efficacy of Korean red ginseng for erectile dysfunction. Int J Impot Res 1995;7:181-186.
4 Fahim MS, Fahim Z, Harman JM, Clevenger TE, Mullins W, Hafez ES. Effect of Panax ginseng on testosterone level and prostate in male rats. Arch Androl 1982;8:261-263.   DOI
5 Ford WC, North K, Taylor H, Farrow A, Hull MG, Golding J. Increasing paternal age is associated with delayed conception in a large population of fertile couples: evidence for declining fecundity in older men. The ALSPAC Study Team (Avon Longitudinal Study of Pregnancy and Childhood). Hum Reprod 2000;15:1703-1708.   DOI   ScienceOn
6 Kim SK, Kwak YS, Kim SW, Hwang SY, Ko YS, Yoo CM. Improved method for the preparation of crude ginseng saponin. J Ginseng Res 1998;22:155-160.   과학기술학회마을
7 Gaedcke F, Steinhoff B, Blasius H. Herbal medicinal products: scientific and regulatory basis for development, quality assurance and marketing authorisation. London: CRC Press, 2003.
8 Hankinson O. Role of coactivators in transcriptional activation by the aryl hydrocarbon receptor. Arch Biochem Biophys 2005;433:379-386.   DOI   ScienceOn
9 Russell LD, Ettlin RA, Hikim AP, Clegg ED. The classification and timing of spermatogenesis. In:. Russell LD, Ettlin RA, Hikim AP, Clegg ED (Eds.). Histological and histopathological evaluation of the testis. Clearwater: Cache River Press, 1990.
10 Johnsen SG. Testicular biopsy score count--a method for registration of spermatogenesis in human testes: normal values and results in 335 hypogonadal males. Hormones 1970;1:2-25.   DOI
11 Zaher TF. Papaverine plus prostaglandin E1 versus prostaglandin E1 alone for intracorporeal injection therapy. Int Urol Nephrol 1998;30:193-196.   DOI   ScienceOn
12 Ernst E, Pittler MH. Yohimbine for erectile dysfunction: a systematic review and meta-analysis of randomized clinical trials. J Urol 1998;159:433-436.   DOI   ScienceOn
13 Drewes SE, Horn MM, Munro OQ, Dhlamini JT, Meyer JJ, Rakuambo NC. Pyrano-isoflavones with erectile-dysfunction activity from Eriosema kraussianum. Phytochemistry 2002;59:739-747.   DOI   ScienceOn
14 Chiou WF, Chen J, Chen CF. Relaxation of corpus cavernosum and raised intracavernous pressure by berberine in rabbit. Br J Pharmacol 1998;125:1677-1684.   DOI   ScienceOn
15 Becker AJ, Stief CG, Schultheiss D, Truss MC, Jonas U. Pharmacological therapy of erectile dysfunction. Urologe A 1998;37:503-508.   DOI
16 Yuan HD, Shin EJ, Chung SH. Anti-diabetic effect and mechanism of Korean red ginseng in C57BL/KsJ db/db mice. J Ginseng Res 2008;32:187-193.   과학기술학회마을   DOI   ScienceOn
17 Qureshi AA, Abuirmeileh N, Din ZZ, Ahmad Y, Burger WC, Elson CE. Suppression of cholesterogenesis and reduction of LDL cholesterol by dietary ginseng and its fractions in chicken liver. Atherosclerosis 1983;48:81-94.   DOI
18 Choi HK, Choi YD, Adaikan PG, Jiang Y. Effectiveness of Korea red ginseng in erectile dysfunction-multi-national approach. J Ginseng Res 1999;23:247-256.   과학기술학회마을
19 Sung H, Kang SM, Lee MS, Kim TG, Cho YK. Korean red ginseng slows depletion of CD4 T cells in human immunodeficiency virus type 1-infected patients. Clin Diagn Lab Immunol 2005;12:497-501.
20 Yun TK, Yun YS, Han IW. Anticarcinogenic effect of long-term oral administration of red ginseng on newborn mice exposed to various chemical carcinogens. Cancer Detect Prev 1983;6:515-525.
21 Kim HS, Jung MW, Jang CG, Park WK, Oh KW. Effects of ginseng total saponin on stress-induced analgesia. Korean J Ginseng Sci 1993;17:22-28.   과학기술학회마을
22 Kim W, Hwang S, Lee H, Song H, Kim S. Panax ginseng protects the testis against 2,3,7, 8-tetrachlorodibenzo-p-dioxin induced testicular damage in guinea pigs. BJU Int 1999;83:842-849.   DOI
23 Hwang SY, Kim WJ, Wee JJ, Choi JS, Kim SK. Panax ginseng improves survival and sperm quality in guinea pigs exposed to 2,3,7,8-tetrachlorodibenzo- p-dioxin. BJU Int 2004;94:663-668.   DOI   ScienceOn
24 Johnson L, Petty CS, Neaves WB. Influence of age on sperm production and testicular weights in men. J Reprod Fertil 1984;70:211-218.   DOI   ScienceOn
25 Holstein AF. Spermatogenesis in the aged--a borderland between normal and pathologic anatomy. Urologe A 1986;25:130-137.
26 Gray A, Feldman HA, McKinlay JB, Longcope C. Age, disease, and changing sex hormone levels in middle-aged men: results of the Massachusetts Male Aging Study. J Clin Endocrinol Metab 1991;73:1016-1025.   DOI
27 Noguchi J, Yoshida M, Ikadai H, Imamichi T, Watanabe G, Taya K. Age-related changes in blood concentrations of FSH, LH and testosterone and testicular morphology in a new rat sterile mutant with hereditary aspermia. J Reprod Fertil 1993;97:433-439.   DOI   ScienceOn
28 Tenover JS, McLachlan RI, Dahl KD, Burger HG, de Kretser DM, Bremner WJ. Decreased serum inhibin levels in normal elderly men: evidence for a decline in Sertoli cell function with aging. J Clin Endocrinol Metab 1988;67:455-459.   DOI
29 Corona G, Razzoli E, Forti G, Maggi M. The use of phosphodiesterase 5 inhibitors with concomitant medications. J Endocrinol Invest 2008;31:799-808.   DOI
30 Plas E, Berger P, Hermann M, Pfluger H. Effects of aging on male fertility? Exp Gerontol 2000;35:543-551.   DOI   ScienceOn
31 Sivalingam S, Hashim H, Schwaibold H. An overview of the diagnosis and treatment of erectile dysfunction. Drugs 2006;66:2339-2355.   DOI   ScienceOn
32 Costabile RA, Mammen T, Hwang K. An overview and expert opinion on the use of alprostadil in the treatment of sexual dysfunction. Expert Opin Pharmacother 2008;9:1421-1429.   DOI   ScienceOn
33 van Dijk MM, de la Rosette JJ, Michel MC. Effects of alpha(1)-adrenoceptor antagonists on male sexual function. Drugs 2006;66:287-301.   DOI   ScienceOn
34 Neaves WB, Johnson L, Porter JC, Parker CR Jr, Petty CS. Leydig cell numbers, daily sperm production, and serum gonadotropin levels in aging men. J Clin Endocrinol Metab 1984;59:756-763.   DOI