• The Plant Pathology Journal
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• v.33 no.1
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• pp.80-86
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• 2017

One of the major problems in strawberry production is difficulty in diagnosis of anthracnose caused by Colletotrichum acutatum or Glomerella cingulata in latent infection stage. We here developed a diagnostic tool for the latent infection consisting of initial culturing of fungi, DNA extraction, synthesis of PCR-amplified probes and microtube hybridization (MTH) using a macroarray. The initial culturing step is convenient to lure the fungi out of the plant tissues, and to extract PCR-inhibitor-free DNA directly from fungal hyphae. For specific detection of the fungi, PCR primers were designed to amplify the fungal MAT1-2 gene. The subsequent MTH step using the PCR products as probes can replace the laborious electrophoresis step providing us sequence information and high-throughput screening. Using this method, we have conducted a survey for a few thousands nursery plants every year for three consecutive years, and finally succeeded in eliminating latent infection in the third year of challenge.

• The Korea Journal of Herbology
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• v.23 no.2
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• pp.111-121
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• 2008

Objectives : The present study was carried out to investigate the effects of Astragali radix on the asthma treatment. EAR-I is an extract of astragali radix cultured for one year and EAR-III is an extract of astragali radix cultured for three years. Methods : Two asthma-induced groups mice group was treated respectively with EAR- I and EAR- III. The other group was not. Each group was analyzed in vivo and in vitro experiments. Results : In asthma-induced mice by OVA treatment, the weight of lung, total cell number of leukocyte and eosinophil in BALF, both EAR- I and EAR- III treated groups were significantly decreased compared to control group. IL-4, IL-5, IL-13, histamine, IgE in BLAF of group treated with both EAR-I and EM-III were significantly decreased compared to control group. In FACS analyzing, granulocytes, $CD3e^-$/$CCR3^+$, $CD3^+$/$CD19^$, $CD3e^+$/$CD69^+$, $CD4^+$, $CD8^+$ and $GR-1^+$/$CD11b^+$ were significantly increased in asthma induced mice group by OVA treatment compared to control group, and decreased in group treated with both EAR- I and EAR-III. Conclusions : The present data proves that extract of astragali radix has an effect on the inhibiting asthma. Moreover, astragali radix cultured for three years was proved to be superior to the one cultured for one year.

• Journal of Yeungnam Medical Science
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• v.40 no.2
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• pp.193-197
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• 2023

Klebsiella pneumoniae is an uncommon cause of septic arthritis in adults. However, late detection can cause serious complications, including joint destruction and immobility. The purpose of this study was to report a case of successfully treated septic arthritis of the hip joint (SAHJ) caused by K. pneumoniae. A 49-year-old female patient presented to our hospital with fever and progressive severe pain in the right hip area. Although there was no abnormality on plain radiographs, ultrasonography revealed diffuse swelling of the right hip joint. Under ultrasonography guidance, the hip joint fluid was aspirated, and Gram staining and culturing were performed. The patient's pain was significantly reduced after the joint aspiration. The Gram staining and culturing revealed gram-negative bacilli, which were subsequently identified as K. pneumoniae. According to the results, systemic intravenous antibiotic (ceftriaxone) was administered without complications, and the patient was discharged on oral antibiotic (ciprofloxacin). Clinical cases of septic arthritis of the knee or sacroiliac joint have been occasionally reported in adults, but cases of SAHJ are rare. Moreover, K. pneumonia-induced SAHJ has not been reported to date. Therefore, we report this very rare case and its successful treatment.

• Journal of Environmental Science International
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• v.22 no.5
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• pp.599-608
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• 2013

Jeju island, which is located along the moving path of typhoon, suffers from flooding and overflow by torrential rain. So abrupt runoff occurring, damages of downstream farm field and shore culturing farms are increasing. In this study, Oaedo stream, one of the mountainous streams on Jeju island, was selected as the basin of study subject and was classified into 3 sub-basins, and after the characteristics of subject basin, the soil erosion amount and the sediment delivery of the stream by land usage distribution were estimated with the use of SATEEC ArcView GIS, the sediment yield amount of 2000 and 2005 was analyzed comparatively. As a result of estimating the sediment yield amount of 2000, the three sub-basins were respectively 12,572.7, 14,080 and 157,761 tons/year. and sediment yield amounts were estimated as 35,172.9, 5,266 and 258,535 tons/year respectively in 2005. The soil erosion and sediment yield amount of 2005 using single storm rainfall were estimated high compared with 2000, but for sub-basin 2, the values rather decreased due to changes in land use, and the land coverage of 2005, since there are many classifications of land usage compared with 2000, enabling to reflect more accurate land usage condition, could deduce appropriate results. It is anticipated that such study results can be utilized as basic data to propose a direction to predict the amount of sediment yield that causes secondary flooding damage and deteriorates water quality within detention pond and grit chamber, and take action against damages in the downstream farm field and shore culturing farms.

• Korean Journal of Environmental Agriculture
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• v.40 no.4
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• pp.270-278
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• 2021

BACKGROUND: Horticultural plant growth under field and/or greenhouse conditions is affected by the climate changes (e.g., temperature, humidity, and rainfall). Therefore investigation of hydroponics on field horticultural crops is necessary for year-round production of the plants regardless of external environment changes under plant factory system with artificial light sources. METHODS AND RESULTS: Common sage (Salvia plebeia), nasturtium (Tropaeolum majus), and hooker chive (Allium hookeri) plants were hydroponically culturing in the plant factory with blue-red-white LEDs (Light-Emitting Diodes) and fluorescent lights (FLs). Leaf numbers of common sage under mixture LED and FL treatments were 134% and 98% greater, respectively than those in the greenhouse condition. In hooker chives, unfolded leaf numbers were 35% greater under the artificial lights and leaf elongation was inhibited by the conventional sunlight compared to the artificial light treatments. Absorption pattern of NO₃-N composition in hydroponic solution was not affected by the different light qualities. CONCLUSION(S): Plant factory system with different light qualities could be applied for fresh-leaf production of common sage, nasturtium, and hooker chive plants culturing under field and/or greenhouse. Controlled light qualities in the system resulted in significantly higher hydroponic growth of the plants comparing to conventional greenhouse condition in present.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.27 no.1
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• pp.94-98
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• 1982

To understand the growth pattern of ginseng plant under shading, the vertical distribution of leaf area, leaf area index (LAI) and specific leaf weight (SLW) and changes in light intensity as affected by planting position were investigated in 3 to 5 years old ginseng plant populations. Light intensity was vertically lowest at about 10cm above the ground and became low at the rear planting position in 3-year-old population. When culturing bed (96cm in width) were divided into three parts at intervals of 32cm from front to rear, the leaf area in 3-year-old population was largest in middle 1/3 part of planting bed. Light intensity affected the SLW positively, but LAI showed no distinct difference among planting positions. The light environment of 4-year-old population was worse than that of 3-year-old population and leaf area and LAI differed greatly among planting positions. In 5-year-old population, leaf dry weight and leaf area of furrow part (that is, the amount of leaves protruded from the plants which were planted in 1st, 2nd or 3rd lines into the furrow) increased. The dry weights of leaves and stem increased considerably as plant became aged, and were distributed mainly in upper layer.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.54 no.4
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• pp.434-444
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• 2021

The decrease in fatness of the Pacific oyster Crassostrea gigas, which consequently results in decrease in the profit of aquaculture industry, has become a source of serious concern in southeast coast of Korea. The ratio of flesh (edible portion) to total oyster weight, commonly called edible portion yield ("suyul" in Korean), have been used as a fatness index for the healthy and valuable state of oyster from the early stage of oyster farming in Korea. More than 360 data sets were collected from early culturing periods (in the 1970s) to the present from approximately 15 published literatures to evaluate the long-term fatness trend of oyster, reared particularly in submerged longline culturing system in Gyeongsangnam-do province. Slight decrease in oyster fatness during the 1970s to 1990s was detected in Tongyeong and Geoje Bays; however, from the 1990s to the present, clear decreasing trends were observed with a decrease of 0.04-0.08% year^-1, especially during harvest season. Oyster mass production per unit area almost doubled within a short period in the early 2000s; however, changes in coastal environment factors inhibited the fattening of shellfish from the mid 1990s. These results indicate that the severe competition in feeding and low biological production in water column might be some convincing reasons for the decrease in fatness of oyster from the 1990s, in Tongyeong and Geoje Bays, Korea.

• Food Science of Animal Resources
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• v.44 no.3
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• pp.499-514
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• 2024

Cultured meat has been gaining popularity as a solution to the increasing problem of food insecurity. Although research on cultured meat started later compared to other alternative meats, the industry is growing rapidly every year, with developed products evaluated as being most similar to conventional meat. Studies on cultured meat production techniques, such as culturing new animal cells and developing medium sera and scaffolds, are being conducted intensively and diversely. However, active in-depth research on the quality characteristics of cultured meat, including studies on the sensory and storage properties that directly influence consumer preferences, is still lacking. Additionally, studies on the combination or ratio of fat cells to muscle cells and on the improvement of microbiota, protein degradation, and fatty acid degradation remain to be conducted. By actively investigating these research topics, we aim to verify the quality and safety of cultured meats, ultimately improving the consumer preference for cultured meat products.

• Korean Journal of Clinical Laboratory Science
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• v.45 no.4
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• pp.154-158
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• 2013

The unnatural dead body refers to a corpse which is suspicious of unusual death and the dead case has been occurred more than 20 thousands times every year during the recent five years and most of them are found decayed. Police Scientific Investigators investigate unnatural dead bodies and its surroundings in all-around way and determine whether the death is involved with a crime, and most of the Scientific Investigators are exposed to a danger to get infected with pathogenic bacteria which are generated during the decaying process of dead body and are mostly to cause serious injuries on human beings. In line with the fact, the present study conducted a bacterial analysis by collecting excretions from 60 dead bodies and culturing the bacteria to evaluate infection risk of the police agents. The study cultured bacteria from 60 bodies and classified pathogenic bacteria of 108 strains, and its main bacteria are found to be them (; Acinetobacter baumannii 20 strains (19%), Pseudomonas aeruginosa 19 strains (18%), E. coli 18 strains (17%), Klebsiella pneumoniae 11 strains (10%), Proteus mirabillis 10 strains (9%), Enterococcus faecium 10 strains (9%), Staphylococcus aureus 9 strains (8%), Bacillus spp. 5 strains (5%), Streptococcus pneumoniae 3 strains (3%), Candida albicans 2 strains (2%), Mycobacterium tuberculosis 1 strains (1%)). The study results are expected to be used as educational data for preventing the Police Scientific Investigator from infections with bacteria or as a minimum data for improving work environment of the agents.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.49 no.3
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• pp.343-350
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• 2016

The sea cucumber Apostichopus japonicus is a commercially valuable aquaculture species in Korea. Aquaculture species require specific nursery culture conditions to increase survival and growth rates. Sea cucumbers hibernate during the high temperatures of summer and during the low temperatures of winter, and suboptimal temperature conditions decrease sea cucumber growth and survival rates. The natural South Korean environment is very unfavorable for culturing sea cucumber; therefore, developing a recirculating aquaculture system (RAS) capable of breeding and growing sea cucumber year-round is necessary. The aim of this study was to investigate growth performance of juvenile sea cucumber in a RAS. Growth and survival rates of juvenile sea cucumber were high during our 24-week experiment. Sea cucumber survival rates were 87.8-93.3%, and specific growth rates were 0.4689-0.7846.