• 한국가축번식학회지
• /
• 제15권3호
• /
• pp.179-187
• /
• 1991

This experiment was carried out to develop a new technique of identifying XX of XY-bearing bisected embryos prior to implantation by immunological method. H-Y antiserum prepared in inbred Wastar female rats by repeated immunization with spleen cells from males of the same strain. The reactivity of H-Y antibody was confirmed by culturing mouse embryos in the medium containing H-Y antiserum and complement obtained from the guinea pig. The optimal condition for the activity of H-Y antibody was also investigated by culturing embryos under the concentraton or affected H-Y antibody was also investigated by culturing embryos under the concentration or affected H-Y antibody and culture rate. However, production of live young or sex rates of male and female from embryos transferred with psudopregnant. The biological test with the morula stage embryos showed that H-Y antibody was formed in all female rats immunized with spleen cell, but it was formed only in 80% female rats immunized with the antigen. When the bisected mouse embryos were cultured in vitro for 5~6 hours in morula stage, of 457 bisected embryos 81.4% of then were developed to the blastocyst stage. When the concentration rate of complement to H-Y antiserum varied from 1.0~5.0${mu}ell$, the lysis-rate of embryo was 19.5 to 67.3%. The concentration rate of complement did not influence the lysis-rate of embryos(P<0.05). The morphology embryos of bisected, zona-free and intact embryos showed the embryos lysis rate of 58.6, 42.7 and 48.5% respectively(P<0.05). Pregnancy rate were 50.0, 45.5 and 57.1% in psudopregnant recipient transferred with bisected, zona-free and intact blastocyst embryos. However, production of live youngs, sexual rate of male or female was 24(50.0:50.0), 22(45.5:55.5) and 36(58.3:41.7)mice, but affected and non affected half embryos with H-Y antiserum treatment was 23.1 and 26.7%. Also production of live youngs and sexual rate was 14(92.9:7.1) and 17(17.6:82.4)mice in affected and non affected half embryos in H-Y antiserum treatment(P<0.05).

• 한국식품저장유통학회지
• /
• 제9권2호
• /
• pp.174-178
• /
• 2002

감초추출물의 농도를 100 ppm, 200 ppm, 300 ppm and 400 ppm로 조절하여 콩을 발아시켜 재배한 콩나물의 아미노산, 유기산 및 유리당 함량을 재배 2일, 3일 및 4일째에 분석하였다. 총 아미노산 함량은 100 ppm에서 3일간 재배하였을 때가 가장 높았고, 또한 재배일수가 길어질수록 aspartic acid 함량은 증가하지만, glutamic acid 함량은 오히려 감소하는 경향을 나타내었다. 총 유리당은 GGE 콩나물의 경우 대조군에 비하여 낮은 함량을 나타내었으며, 그러나 재배일수가 증가할수록 그 함량은 많아졌다 Sucrose함량은 재배일수가 길어질수록 감소하였으나, 다른 유리당은 오히려 증가하였다 총 유기산은 재배일수가 증가할수록 많은 함량을 나타내었으며, 200 ppm으로 재배한 콩나물이 가장 많은 함량을 나타내었다. 결론적으로 감초추출물은 콩나물의 품질증진을 위한 아미노산 및 유기산 함량을 증가시키는 발아 및 재배수로서 효과적일 것으로 판단된다.

• 농업과학연구
• /
• 제45권4호
• /
• pp.715-723
• /
• 2018

The present study was done to investigate the effect of co-culturing muscle satellite cells (MSCs) and intramuscular preadipocytes (IPs) on the differentiation of adipocytes and muscle cells isolated from Korean native cattle. MSCs and IPs were single-cultured in 10% fetal bovine serum/Dulbecco's modified Eagles medium (FBS/DMEM) for 48 h followed by culturing in 5% FBS/DMEM as the growth media. Then, the growth media was replaced by differentiation media composed of 2% FBS/DMEM without any additives for the single- or co-culture of muscle cells and intramuscular adipocytes to induce the differentiation of both cell types. Cell differentiation was measured by morphological investigation and cytosolic enzyme analysis of glycerol-3-phosphate dehydrogenase (GPDH) for the adipocytes and creatine kinase (CK) for the muscle cells. In the morphological test, the presence of muscle cells did not stimulate adipocyte differentiation showing more differentiation of the adipocytes in the single-culture compared to the co-culture condition. However, the differentiation of muscle cells was promoted by adipocytes in the co-culture. The results of the enzymatic analysis were highly associated with the morphological results with a statistically higher GPDH activity (p < 0.05) appearing in the single-culture than in the co-culture, whereas the opposite was true for the CK activity of the muscle cells (p < 0.05). By manipulating in vivo the milieu using a co-culture, we could detect the difference in the rate of cell differentiation and suggest that a co-culture system is a more reliable and precise technique compared to a single-culture. Further studies on various co-culture trials including supplementation of differentiating substances, gene expression analysis, etc. should be done to obtain practical and fundamental data.

• 한국해양학회지:바다
• /
• 제17권4호
• /
• pp.262-269
• /
• 2012

국내 연안의 다양한 양식장의 퇴적물과 양식생물에 대하여 다이옥신류(PCDD/Fs), 다이옥신류피씨비(DLPCBs), 및 브롬화방염제(PBDEs)의 잔류수준을 조사하고 분포를 평가하였다. 양식장 퇴적물 중 PCDD/Fs, DLPCBs, PBDEs는 각각 0.03~2.9(평균${\pm}$표준편차, $1.2{\pm}0.89$) pg $WHO_{2005}$-TEQ $g^{-1}$ 건중량(dw), 불검출~1.1($0.09{\pm}0.19$) pg $WHO_{2005}$-TEQ $g^{-1}$ dw, 불검출~16.6($2.96{\pm}3.53$) ng $g^{-1}$ dw 수준으로 검출되었다. 또한 양식생물 체내 함유량은 각각 불검출~0.24($0.07{\pm}0.06$) pg $WHO_{2005}$-TEQ $g^{-1}$ 습중량(ww), 불검출~0.11($0.04{\pm}0.04$) pg $WHO_{2005}$-TEQ $g^{-1}$ ww, 0.05~0.29($0.13{\pm}0.06$) ng $g^{-1}$ ww 수준이었다. 국내 연안 중 동해안과 남해안에 위치한 양식장 퇴적물 중 총다이옥신류(PCDD/Fs+DLPCBs)와 PBDEs의 농도는 서해안보다 약 4~7배 높은 수준을 보였다. 퇴적물에서는 총다이옥신류에 대한 PCDD/Fs 기여율이 대부분(평균 94%)을 차지했지만, 양식생물에서는 DLPCBs가 약 33% 수준으로 퇴적물에 비해 높은 기여율을 보였다. PBDEs 중에서는 양식장 퇴적물과 생물 모두에서 BDE209가 가장 높게 검출되었다. 이전 연구와 비교했을 때, 국내연안 퇴적물과 양식생물 중 PCDD/Fs, DLPCBs, PBDEs의 잔류수준은 대체로 감소하는 추세에 있는 것으로 판단된다.

• 수산경영론집
• /
• 제16권1호
• /
• pp.91-124
• /
• 1985

Mariculture is contrasted with inland aqua-culturing fisheries. It is defind as the Industry of rearing Aquaorganism in limited coastal area relatively shallow in depth. Then, It's coming into being realization of Mariculture in it is long in history that Mariculture was realized in Korea. But it is from the early part of 1960s, that this industry has normally developed. Owing to 200 miles economy-zone problems of coastal countries, the development of deep sea fishing was limited, so the Korean Government has now appreciated the importance of cultured industries in the field of coastal fisheries. And the Korean Mariculture the output of which was only 18, 000 M/T in '60s attained 540, 000M/T in 1980s, has now occupied its relative importance in Korean Fisheries Industry. So the purpose of this report is to suggest the prospect of technical development of mariculture in the future of Korea, through the analysis of the various problems that affect upon the individual management '||'&'||' fishing ground utilization, along with the appreciation of "how to extend of those technical innovation" and "how the fishermen's technique level is extended at this stage. According to this study, the result is summarized as follows. First, Maricultural technique is classified into 8 sub-techniques as follows, as shown in fig. 1.Fig. 1. The Formation structure of mariculture technique Second, the change of technical method of mariculture in coastal area of Korea has made as 5 stages; 1) Scattering of culturing organism 2) Culturing by putting stone and installing bamboo 3) Culturing by installing rope and seeding 4) Culturing of putting objectives in cages 5) Culturing fish by feed Third, the maricultural fisheries of Korea has about 70 years long in history. It began from 1910s. But at that time there was no special technique in aquaculture and its technique was confined in searching out the object of species. The species was laver, oyster ect.Forth, although realization of mariculture in Korea has been long time, it is of late from 1960s that this has been industrial with normal development, and its technique of mariculture has mainly has developed from 1970s. Its result not only contributed to the high growth in Korean ecconomy along with the well balanced development between industires, but also it played a great role for the resolution of nation's food problem. Especially maricultural production has shown its sustained annual increase of 13.8% during the last 20 years. So the portion of mariculture among total fisheries stucture was extended from 4.1% in the early 1960s to 22.4% in 1980s.Fifth, it could be safely said that such development in maricultural field is resulted from the activity of aquacultural institutes such as Fisheries Reseach '||'&'||' Development production of major kinds such as Oyster, Sea-mustard, and Laver etc. As well as in the innovation of aquaculturing method with synthetic fiber utilization. FRDA has played important role in the efficient propargation of new aquacultural technique.Sixth, as for the change in aquaculture structure and its during period between 1970s and 1980s, the private management participation shown 25% increase from household number of 45, 173 to 56, 268 in total number. And in the respect of the management scale, of their management decreased, while it showed an increase in relative large scale management, the increase over 3 employees compared with other fisheries field between '70s and 80s. This must be an major trait to be recorded, Now the data above mentioned are shown as in table 1 and 2.Table 1. The maricultural fishing ground development situation in 982.Table 2. The mariculture management as seen in the employmnet size in high seasion.Owing to the technical innovation, of the mariculture in coastal area new income of fishermen increased and it also is true that the number of fishermen participating in its industrialization increased. But the problem being from now on is the self-discharge of the destruction fishing ground considered resulted from rapid expansion in aquaculture industry and the preventive system of sentility of fishing ground. sentility of fishing ground.

• Applied Biological Chemistry
• /
• 제49권1호
• /
• pp.1-6
• /
• 2006

Lactobacillus acidophilus KCCM 32820과 Propionibacterium freudenreichii KCCM 31227을 MRS, RCM, MRS와 RCM 합성배지 및 whey broth에 배양하여 OD(Optical Density), pH, 총산도, 생균수 등의 생육특성을 조사하였다. L. acidophilus KCCM 32820을 NRS 배지에서 배양시 균 증식, 총산도 값의 증가율 및 pH 저하율은 9-21시간에 가장 컸으며, P. freudenreichii KCCM 31227을 RCM 배지에서 배양시에는 균의 증식, 총산도 값의 증가율 및 pH 저하율은 24-60시간에 가장 컸다. 또한 합성배지에서 P. freudenreichii KCCM 31227의 pH와 총산도 변화는 18-54시간에 가장 높았다. When broth에서는 L. acidophilus KCCM 32820이 P. freudenreichii KCCH 31227보다 균의 성장이 좋았으며, 두 균 모두 6%보다 12% whey broth에서, 고압증기멸균보다 저온살균한 whey broth에서 높은 균수를 나타냈고, 저온살균한 12% whey broth에서 증식이 가장 높았다.

• 전자공학회논문지CI
• /
• 제49권2호
• /
• pp.29-37
• /
• 2012

본 논문에서는 화훼 온실에 Ubiquitous 기반의 Sensor Network와 다양한 센서를 설치하여, 최적의 자동 온실 환경을 구현, 그 결과를 평가하였다. 이를 위해, 온실에서의 다양한 생장환경 정보를 실시간 수집/분석하여, 기존의 최적 생장환경 권장 수치를 기준으로, 최적의 생장 환경을 유지하도록 설계하였다. 실험 대상인 화훼온실의 농작물 생장결과를 측정하기 위해, 환경변화에 따른 최적의 생장환경을 유지하도록, 온실 시설물을 제어하는 시스템을 구현하고, 이를 분석하였다. 기존의 온실 관리 방법과는 달리, 본 논문에서 적용된 시스템은 사용자가 원격에서 생장환경 모니터링 및 시설제어가 가능하도록 설계되었다. 또한, 화훼의 생산량 및 품질 향상을 위해 자동 온습도 조절기와 인공광원 등을 설치하였다. 그 결과 온실 시설물과 인공광원의 제어를 통해 화훼 생산량, 품질, 노동력 및 난방비에서 기존보다 상당히 향상됨을 볼 수있었다. 실험결과, u-화훼 시스템이, 날씨 변화 및 이상 기상 상황 등이 발생했을 경우, 사용자에게 SMS를 통해 경고 메시지를 보냄으로써 실시간적으로 이상 날씨 변화 상황에 원격으로 제어, 대응하여, 최적의 생장환경을 유지, 농가의 소득을 극대화할 수 있음을 입증하였다.

• 한국비블리아학회지
• /
• 제31권1호
• /
• pp.285-310
• /
• 2020

국내 기록관리학 분야에서 국가기록원 및 기록관의 기록정보콘텐츠에 대한 이용자 인식에 관한 연구들은 다수 수행되었지만, 창작 목적 디지털 아카이브 콘텐츠 활용에 대한 이용자들의 인식 조사는 수행 된 바가 없었다. 이에 본 연구는 한국콘텐츠진흥원에서 운영 중인 창작 목적 디지털 아카이브 '컬처링(Culturing)' 웹 사이트의 콘텐츠 활용에 대한 이용자 인식 조사를 실시하였다. 설문을 통한 인식조사는 이용자들의 컬처링 사이트 콘텐츠들에 대한 관심 정도와 콘텐츠 유형 및 주제 분야별 예상 이용선호도와 예상 도움정도 등에 대하여 이루어졌다. 설문데이터의 분석은 연구대상자의 일반적인 사항과 인식과 관련된 평균값을 비교·분석하기 위하여 One way ANOVA를 사용하였으며, 동(同) 사항 및 인식과 관련된 명목 변수 항목들 간의 연관성을 알아보기 위하여 교차분석을 실시하였다. 이러한 분석결과를 토대로 한 제언점은 다음과 같다. 즉, ① 카드스토리 등의 콘텐츠 유형 및 콘텐츠 내용의 전문성 제고를 포함한 정보 자료의 확충, ② 이용자 종사분야별 맞춤형(Customizing) 인터페이스 설계와 가독성 향상 및 모바일 플랫폼 전용 사이트 개발, ③ 대형 포털사이트와 창작자 집단을 대상으로 한 사이트 홍보의 강화 등이다.

• Transactions on Electrical and Electronic Materials
• /
• 제15권5호
• /
• pp.275-278
• /
• 2014

We deposited TiAgN and ZrAgN nanocomposite coatings on pure Titanium specimens, by using arc ion plating (AIP) with single alloy targets. TiAg ZrAg alloy targets of 5 wt.%, 10 wt.% silver content by vacuum arc remelting (VAR), followed by homogenization for 2 hours at $1,100^{\circ}C$ in non-active Ar gas atmosphere and characterized these samples for morphology and chemical composition. We investigated the biocompatibility of TiAg and ZrAg alloys by examining the proliferation of L929 fibroblast cells by MTT test assay, after culturing the cells ($4{\times}10^4cells/cm^2$) for 24 hours; and exploring the antibacterial properties of thin films by culturing Streptococus Mutans (KCTC3065), using paper disk techniques. Our results showed no cytotoxic effects in any of the specimens, but the antibacterial effects against Streptococus Mutans appeared only in the 10 wt.% silver content specimens.

• 한국환경과학회지
• /
• 제21권7호
• /
• pp.779-785
• /
• 2012

Several tests and experimental work have been done for identifying the best growth conditions and accumulated amount of lipid moiety in B. braunii, a microalga(UTEX 572) in terms of media composition. The specific growth rate was found to be the highest at 0.15 g/L-day when the phosphorus concentration was doubled with the other ingredients at the normal level. Experiments for varied media compositions revealed that the accumulation of lipid was the highest at 48% (dry cell weight based) in the nitrogen deficient medium and its corresponding specific growth rate was comparative to that in the normal BG 11 medium. In the bubble column experiments, carbon dioxide containing air produced four times more cell mass than air only. Light and glucose addition also enhanced cell mass with maximum, 1.8 g/L and accordingly 42% of lipid composition, which turned out to be a better strategy for higher lipid-producing microalgal culture.