• 한국미생물·생명공학회지
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• 제25권1호
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• pp.37-43
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• 1997

Triphenylmethane was decolorized rapidly by enterbacter cloacae MG 82 at initial reaction time. The spheroplast showed higher activity of triphenylmentane decolorization than that of intact cell suspension. The outer part of the bacterial cell envelope and the peptidoglycan are important for the function of transport barrier of triphenylmethane. In intact cell, decolorization activity was higher at 37$\circ $C than at $\circ $C, indicating that triphenylmethane decolorization is due to the enzyme reaction. Culture filtrate showed no decolorization activity, while cell-free extract appeared high activity of 1.45 units, clearly showing that decolorization activity was due to the cell-free extract. Comparing decolorization activities of cell fractions, it was found that decolorization activity was located at the compartment of cytoplasmic membrane. The enzyme activity was also shown to be Mg$^{++}$-dependent. The optimum pH and temperature of enzyme activity were 7.0 and 50$\circ $C, respectively. The thermostability of this enzyme at 35$\circ $C was kept to 58% for 3 hours.

• 한국환경성돌연변이발암원학회지
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• 제16권2호
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• pp.117-123
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• 1996

This study was carried out to examine the mechanism of Arsenic cytotoxicity through several in vitro test systems. Dose-dependent decrease of cell survival by Arsenic was observed by colony forming assay. Arsenic was weak mutagenic in inducing HGPRT point mutation in CHO cells. The frequency of chromosomal aberrations increased in a dose-dependent manner and the most frequent type of chromosomal aberrations induced by Arsenic were chromatid type deletions. U!trafiltrates of culture media from CHO cells treated with Arsenic induced sister chromatid exchanges(SCE) in CHO cells and Arsenic was able to induce lipid peroxidation in CHO cells. The results suggested that the ultrafiltrates of media from CHO cells treated with Arsenic contain clastogenic factor(CF) and Iipid peroxidation might be involved in the formation of CF.

• Toxicological Research
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• 제15권1호
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• pp.89-93
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• 1999

2,3,7,8-Tetrachlorodibenzo-p-dioxin(TCDD) is known to induce cytochrome p450 1A1 and to activate c-Src kinase and p21 Ras. This study examined the molecular interactions of adaptor proteins including Shc, Grb2, and Sos in rat primary hepatocytes and their relationship to the induction of CYP1A1 by TCDD. TCDD induced CYP1A1 level and EROD activity in a dose-dependent mode. Sos/Grb2 association isincreased by TCDDㅑㅜ a dose dependent mode. Tyrosine phosphorylated Shc, mainly p152, onloads to Grb2/Sos complex upon TCDD stimulation. The electrophoretic mobility shift of Sos is showed by TCDD. These results indicate that TCDD modulated the molecular interaction features of adaptor compoes proteins including Shc, Grb2, and Cnl in early signaling pathway of TCDD-mediated CYP 1A1 induction of rat primary hepatocyte.

• 한국항해항만학회지
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• 제30권7호
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• pp.579-583
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• 2006

The purpose of this paper is to study a relationship between alliance success factors and performances of strategic alliance. In order to achieve the purpose of this research, factor analysis, reliability and validity and regression method are used. In conclusion, alliance success factors can be divided as mutual complementarity and information sharing factor, sharing and mutual agreement of vision and goal factor, performance management factor and culture and organization factor. According to regression results, all of four factors affects significantly dependent variables. Among them, mutual complementarity and information sharing mostly affects each dependent variable.

• 약학회지
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• 제43권1호
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• pp.85-90
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• 1999

FTY720, a novel immunosuppressant, elevated the level of endogenous sphingoid bases in a dose-dependent manner within 3 hr in $LLC-PK_1$ cells. The relative molar ratio of sphingoid bases expressed as sphingosine/sphinganine (SPN/SPA), a biomarker of altered sphingolipid biosynthesis, in $10{\;}{\mu}M$ of FTY720 showed tow-fold increase as compared with the one in control culture. FTY720 under the serum-free medium condition increased only cytosolic free sphingosine concentration, not sphinganine concentration in a time-dependent manner over the 8 hr incubation under the same condition as in serum free cultures, the SPN/SPA ratio began to fluctuate and the number of floating cells as an indicator of cytotoxicity was increased 8 hr after the addition of FTY720 to cultured cells. These results suggest that the process of FTY720-induced cell death in $LLC-PK_1$ cells.

• Journal of the Optical Society of Korea
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• 제14권1호
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• pp.55-62
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• 2010

We introduce a new method for lens design that combines an eyepiece with the finite model eye, to make a corrected version of the accommodation-dependent Navarro eye. The optical system that we designed, which includes a human eye, takes into account the aberration of the eye and increases the performance of the image in the retina. In the design results, for the optimized eyepiece combined with the corrected Navarro eye, visual acuity is 1.40. Compared with the existing method of eyepiece design using inverse ray tracing with the corrected Navarro eye, MTF value was recorded as 0.079 to 0.283 at 160 lp/mm and visual performance was improved.

• International journal of advanced smart convergence
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• 제13권2호
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• pp.94-102
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• 2024

This study examines the factors influencing loyalty to cultural arts events using data from the Survey Report on National Culture and Arts Activity in 2022 and 2023. The dependent variable is a binary variable representing the intention to revisit in the future, which serves as a proxy for loyalty. Given that the dependent variable is binary, the logit model specification is employed to estimate the average marginal effects. The estimation results indicate that audience satisfaction exerts the strongest influence on loyalty in both years. It can be observed that participation in cultural arts events is the second most important variable in determining loyalty. This suggests that the government should support the expansion of the scope of these activities and the diversification of programs in order to facilitate greater participation in a wider range of cultural arts activities.

• International Journal of Industrial Entomology and Biomaterials
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• 제27권1호
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• pp.166-179
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• 2013

In India, forest based insect enterprises has never been linked up with any forest management activity, either as a forest conservation strategy or to reduce the poverty in forested area. This investigation indicated that when forest dependent people are associated with forest insect industry (FII) like forest sericulture, lac culture or apiculture; this income generating activity links livelihood with forest conservation, and generates a viable model of collaborative forest management (CFM). In this model different stakeholders work together as a coherent entity for unified goal of managing the forest for well-being of the poor people in fringe areas. Article summaries and evaluates the prospect of India specific forest insect industry, and discusses how and to what extent integration of FII could be a viable livelihood component in CFM to conserve the forest and insect biodiversity. We analysed a case study on forestbased rearing of tropical tasar silkworm rearing in Central India from CFM perspective. Arguments in this communication are intended to provide forest managers and policy-makers with necessary input to consider location specific FII in CFM mode to provide a continuous source of small income to forest dependent people to ensure long lasting success of their forest management endeavours.

• 한국생활과학회지
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• 제18권6호
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• pp.1181-1198
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• 2009

The purposes of this study were to find the general trends of individuals, marital relation, migrated-cultural variables and abuse, and to analyze the effects of the individual, marital relation, and migrated-cultural variables on the abuse. The subjects were 174 immigrant women. The data was analyzed with frequency, t-test, ANOVA and multiple regression by SPSS. The major findings were as follows; Physical abuse amongst immigrant women was significantly different according to residence and income factors, psychological abuse was dependent on residence and native country, sexual abuse was dependent on education level, and total abuse was dependent on residence and native country. The level of abuse (range 1-4) was 2.07, Psychological abuse was the highest with 2.23, physical abuse was next with 1.92 and sexual abuse was 1.78. All the abuse types were influenced by the husband's alcohol behavior, communication efficiency, marital conflict, and knowledge of Korean culture. In conclusion, it is important to protect immigrant women who have been abused. So understanding, reasonable coping, prevention programs and counseling of abuse victims were provided to immigrant women.

• Molecules and Cells
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• 제39권9호
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• pp.705-713
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• 2016

The efficiency of Agrobacterium-mediated transformation in plants depends on the virulence of Agrobacterium strains, the plant tissue culture conditions, and the susceptibility of host plants. Understanding the molecular interactions between Agrobacterium and host plant cells is crucial when manipulating the susceptibility of recalcitrant crop plants and protecting orchard trees from crown gall disease. It was discovered that Arabidopsis voltage-dependent anion channel 1 (atvdac1) mutant has drastic effects on Agrobacterium-mediated tumorigenesis and growth developmental phenotypes, and that these effects are dependent on a Ws-0 genetic background. Genetic complementation of Arabidopsis vdac1 mutants and yeast porin1-deficient strain with members of the AtVDAC gene family revealed that AtVDAC1 is required for Agrobacterium-mediated transformation, and there is weak functional redundancy between AtVDAC1 and AtVDAC3, which is independent of porin activity. Furthermore, atvdac1 mutants were deficient in transient and stable transformation by Agrobacterium, suggesting that AtVDAC1 is involved in the early stages of Agrobacterium infection prior to transferred-DNA (T-DNA) integration. Transgenic plants overexpressing AtVDAC1 not only complemented the phenotypes of the atvdac1 mutant, but also showed high efficiency of transient T-DNA gene expression; however, the efficiency of stable transformation was not affected. Moreover, the effect of phytohormone treatment on competence to Agrobacterium was compromised in atvdac1 mutants. These data indicate that AtVDAC1 regulates the competence of Arabidopsis to Agrobacterium infection.