• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.6
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• pp.371-374
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• 2002

Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)［P(3HB/V)］, by fed-batch culture of recombinant Escherichia coli harboring a plasmid containing the Alcaligenes latus polyhy-droxyalkanoate (PHA) biosynthesis genes, was examined in two pilot-scale fermentors with air supply only, In a 30 L fermentor having a XLa value of 0.11 S­$^1$, the final P(3HB/V) concentration and the P(3HB/V) content obtained were 29.6 g/L and 70.1 wt%, respectively giving a productivity of 1.37 g P(3HB/V)/L-h. In a 300 L fermentor having a XLa of 0.03 S­$^1$, the P(3HB/V) concentration and the P(3HB/V) content were 20.4 g/L and 69 wt%, respectively giving a productivity of 1.06g P(3HB/V)/L-h. These results suggest that economical production of P(3HB/V) is possible by fed-batch culture of recombinant E. coli in a large-scale fermentor having low KLa value.

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.123-123
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• 2005

• East Asian mathematical journal
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• v.35 no.2
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• pp.163-172
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• 2019

In recent years, the term core literacy has been popular in educational circles all over the world, and there are more and more research on core literacy. At the same time, the mathematical culture has also been highly valued on a global scale. The mathematical culture is a part of college students' cultural quality, and then, what is the connection between the mathematical culture and mathematical core literacy? How to improve the mathematical core literacy of contemporary college students? This paper gives the corresponding answers to these two questions. By illustrating the concrete implementation of the course "Mathematical Culture" offered by Yanbian University, this paper discusses various measures for cultivating students' core qualities in Chinese universities. It must be useful and promote the research on mathematical core literacy for the educators in various countries.

• Quality Improvement in Health Care
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• v.30 no.1
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• pp.105-119
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• 2024

Purpose: This study aimed to develop a survey instrument to assess the Patient Safety Culture in Korean hospitals and evaluate its validity and reliability. Methods: A preliminary instrument was developed through a literature review, focus group interviews, content validity testing, and pretesting for face validity. A total of 467 hospital employees participated in the psychometric testing. Validity and reliability assessments included content validity, construct validity, criterion-related validity, and internal consistency. Results: The Korean Patient Safety Culture Survey Instrument comprised 35 items across seven factors: leadership, patient safety policy and procedure, patient safety improvement system, teamwork, non-punitive environment, patient safety knowledge and attitudes, and patient safety priority. These seven factors contributed 60.98% of the variance of the total scale. Cronbach's alpha for internal consistency was .93; the seven factors ranged from .66 to .91. Conclusion: The results of this study showed that the Korean Patient Safety Culture Survey Instrument is reliable, valid, and suitable for measuring patient safety culture in Korean hospitals.

• Journal of Oriental Neuropsychiatry
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• v.28 no.3
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• pp.207-216
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• 2017

Objectives: To simplify the mindfulness scale and to ensure reliability and validity of the scale. Methods: To develop scale items using the original scale and to identify factor structure of the scale, exploratory factor analysis was conducted based on results of factor analysis, the short-form 5-factor mindfulness scale item was selected. Internal consistency coefficient was calculated and confirmatory factor analysis was conducted to verify reliability of the short-form scale. Finally, to verify validity of the abbreviated scale, we conducted correlation analysis with the abbreviated scale and the mindfulness scale (FFMQ). Results: As with the original scales, scaled scales were composed of 5 factors and consisted of 15 items in total. Factor analysis revealed factor loadings were adequate and reliability and validity were secured. Conclusions: It provided that mindfulness shortening scale validation can be used more simply to measure mindfulness in the clinical scene.

• Journal of Korean Academy of Nursing
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• v.40 no.6
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• pp.820-830
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• 2010

Purpose: The purpose was to develop a preliminary scale to measure Korean adolescents' health behaviors through a qualitative approach, to evaluate the scale psychometrically, and to develop a final scale. Methods: Participants were 61 adolescents for qualitative interviews and 1,687 adolescents for the psychometric evaluation. Procedure included content analysis of interviews to identify health behavior categories for Korean adolescents, pre-test to confirm that preliminary scale items were understandable, content validity by an expert panel, development of the web-based computer-assisted survey (CAS), and psychometric analysis to determine reliability and validity of the final scale. Results: A final scale was developed for both paper-and-pencil and CAS. It consisted of 14 health behaviors (72 items), including stress and mental health (10), sleep habits (5), dietary habits (12), weight control (4), physical activity (4), hygiene habits (5), tobacco use (5), substance use (2), alcohol consumption (4), safety (4), sexual behavior (9), computer use (3), health screening (4), and posture (1). Conclusion: The scale's strong points are: 1) Two thirds of the final scale items are Likert scale items, enabling calculation of a health behavior score. 2) The scale is appropriate to Korean culture. 3) The scale focuses on concrete health behaviors, not abstract concepts.

• Journal of Plant Biotechnology
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• v.5 no.1
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• pp.1-6
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• 2003

The adventitious root of Panax ginseng C.A. Meyer is regarded as an efficient alternative to cell culture or hairy root culture for biomass production due to its fast growth and stable metabolite production. To determine optimal culture conditions for the bioreactor culture of ginseng roots, experiments have been conducted on physical and chemical factors such as bioreactor type, dissolved oxygen, gas supply, aeration, medium type, macro- and micro-elements, medium supplement during culture period, sucrose concentration, osmotic agents, medium pH and light. Elicitation is a key step to increase ginsenoside accumulation in the adventitious roots but biomass growth is severely inhibited by elicitor treatment. To obtain high ginsenoside content with avoiding biomass decrease, we applied two-stage bioreactor culture system. Ginseng adventitious roots were cultured for 40 days to maximize biomass increase followed by elicitation for 7 days to enhance ginsenoside accumulation. We also experimented on types and concentrations of jasmonate to determine optimal elicitation methods. In this paper, we discussed several factors affecting the root propagation and ginsenoside accumulation. Based on the results obtained from previous experiments we have established large-scale bioreactor system (1 ton-10 ton) for the efficient production of ginseng adventitious roots and bioactive compounds including ginsenoside. Still, experiments are on going in our laboratory to determine other bioactive compounds having effects on diet, high blood pressure, DPPH elimination and increasing memories.

• Safety and Health at Work
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• v.6 no.2
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• pp.97-103
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• 2015

Background: This paper describes the development of a scale for measuring safety climate. Methods: This study was conducted in six manufacturing companies in Iran. The scale developed through conducting a literature review about the safety climate and constructing a question pool. The number of items was reduced to 71 after performing a screening process. Results: The result of content validity analysis showed that 59 items had excellent item content validity index (${\geq}0.78$) and content validity ratio (> 0.38). The exploratory factor analysis resulted in eight safety climate dimensions. The reliability value for the final 45-item scale was 0.96. The result of confirmatory factor analysis showed that the safety climate model is satisfactory. Conclusion: This study produced a valid and reliable scale for measuring safety climate in manufacturing companies.

• Journal of Korean Academy of Nursing Administration
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• v.4 no.2
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• pp.363-385
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• 1998

The purpose of this study is to investigate the relationship between organizational culture types and organizational effectiveness in the hospitals and to identify the cultural and organizational characteristics of the hospitals with high organizational effectiveness. Data were collected from May 12 to June 14. 1997 through questionnaire taken by 1.118 nurses working in 10 hospitals with more than 800 beds and from annual reports published by the hospitals. The instruments were used for collecting the data: Organizational Culture Questionnatire and Organizational Characteristics Questionnatire developed by the researcher. Mowday's Organizational Commitment Questionnaire. Taylor & Bovver's General Satisfaction Scale. The Results were as follows: 1. The meta culture of the hospital organizations was the conservative culture. 2. There were significant differences of the four organizational cultural types - affiliative culture. innovative culture. conservative culture. task culture among the hospitals(p=.00). 3. The hospital organizations were classified in to three cultural patterns. each of which had similar cultural composition. on the basis of the scores indicating the similarity and difference of the foul' organizational cultural types among the hospitals. The organization of each group represents conservative- dominant culture. innovative-dominant culture and competitive culture. 4. Nurses' organizational commitment and job satisfaction were significantly different among the cultural patterns(p=.00). In other words. the hospitals with innovative-dominant culture showed higher organizational commitment and job satisfaction than ones with conservative-dominant culture and competitive culture. And also. the growth rate of outpatients and inpatients were significantly different among the cultural patterns(p<.05). The hospitals with innovative -dominant culture showed higher growth rate of outpatients and inpatients than ones with conservative-dominant culture and competitive culture. 5. The hospitals with conservative-dominant culture and competitive culture showed higher level of centralization than ones with innovative -dominant culture(p=.00) And the hospitals with competitive and innovative-dominant culture showed higher level of communication than those with conservative-dominant culture(p=.00) Finally. the hospitals with innovative-dominant culture showed higher level of managerial strategy than those with conservative-dominant and competitive culture. among which the latter showed higher level of managerial strategy than the former(p=.00).

• Horticultural Science & Technology
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• v.16 no.3
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• pp.358-360
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• 1998

Series of in vitro experiments in Lilium callosum were conducted to investigate efficient multiplication through finding the optimal cultural environment, and organogenic capability of cultural explants, and then to determine the progressive method for enhancing bulblet growth in Lilium callosum scale culture. Twenty-four hr photoperiod was most effective for the growth of bulblet and the formation of other organs. Optimum light intensity for bulblet growth was 2,500~5,000 Lux. When bulbets were subcultured, growth of bulblets were enhanced by removing excessive leaf blade. Number of bulblets per scale increased as mother scale size increased, whereas diameter of bulblet from the small size mother scale increased. Bulblet formation and development was induced when explants were placed above the medium to be exposed to more light.