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Pilot Scale Production of Poly (3-Hydroxybutyrate-co-3-hydroxy-valerate) by Fed-batch Culture of Recombinant Escherichia coli  

Park, Jong-il (Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center)
Lee, Sang-Yup (Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center, Center for Vltramicrochemical Process Systems, Korea Advanced Institute of Science and Technology)
Kyungsup Shin (Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center)
Lee, Woo-Gi (Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center)
Park, Si-Jae (Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center)
Chang, Ho-Nam (Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center)
Chang, Yong-Keun (Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.7, no.6, 2002 , pp. 371-374 More about this Journal
Abstract
Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)［P(3HB/V)］, by fed-batch culture of recombinant Escherichia coli harboring a plasmid containing the Alcaligenes latus polyhy-droxyalkanoate (PHA) biosynthesis genes, was examined in two pilot-scale fermentors with air supply only, In a 30 L fermentor having a XLa value of 0.11 S­$^1$, the final P(3HB/V) concentration and the P(3HB/V) content obtained were 29.6 g/L and 70.1 wt%, respectively giving a productivity of 1.37 g P(3HB/V)/L-h. In a 300 L fermentor having a XLa of 0.03 S­$^1$, the P(3HB/V) concentration and the P(3HB/V) content were 20.4 g/L and 69 wt%, respectively giving a productivity of 1.06g P(3HB/V)/L-h. These results suggest that economical production of P(3HB/V) is possible by fed-batch culture of recombinant E. coli in a large-scale fermentor having low KLa value.
Keywords
P(3HB/V); recombinant E. coli; pilot-scale fermentor; oxygen transfer rate;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By SCOPUS : 14
연도 인용수 순위
1 High level production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by fedbatch culture of recombinant Escherichia coli. /
[ Choi, J.;S. Y. Lee ] / Appl. Environ. Microbiol.
2 Cloning of the Alcaligenes latus polyhydroxyalkanoate biosynthesis genes and use of these genes for enhanced production of poly(3-hydroxybutyrate) by recombinant Escherichia coli. /
[ Choi, J.;S. Y. Lee;K. Han ] / Appl. Environ. Microbiol.
3 Fermentative production of SCL-PHAs. /
[ Lee, S. Y.;S. J. Park;Y. Doi(ed.);A. Steinbuchel(ed.) ] / Biopolymers.
4 Plastic bacteria Progress and prospects for polyhydroxyalkanoate production in bacteria. /
[ Lee, S. Y. ] / Trends Biotechnol.   DOI   ScienceOn
5 Measurement of KLa by a gassing-in method with oxygenenriched air /
[ Chang, H. N.;B. Halard;M.-Y. Murray ] / Biotechnol. Bioeng.   DOI
6 Metabolic analysis of poly(3-hydroxybutyrate) production by recombinant Escherichia coli. /
[ Wong, H. H.;R. J. van Wegen;J. Choi;S. Y. Lee;A. P. J. Middelberg ] / J. Microbiol. Biotechnol.   과학기술학회마을
7 Biosynthesis and fermentative production of SCL-MCL-PHAs. /
[ Lee, S. Y.;S. J. Park;Y. Doi(ed.);A. Steinbuchel(ed.) ] / Biopolymers.
8 Mass transfer in mechanically agitated gas-liquid contactors. /
[ Mehta, V. D.;M. M. Sharma ] / Chem. Eng. Sci.   DOI   ScienceOn
9 A rapid gas chromatographic method for the determination of poly--hydroxybutyric acid in microbial biomass. /
[ Braunegg, G.;B. Sonnleitner;R. M. Lafferty ] / Eur. J. Appl. Microbiol. Biotechnol.   DOI
10 Bacterial polyhydroxyalkanoates. /
[ Lee, S. Y. ] / Biotechnol. Bioeng.   DOI   ScienceOn
11 Process analysis and economic evaluation for poly(3-hydroxybutyrate) production by fermentation. /
[ Choi, J.;S. Y. Lee ] / Bioprocess Eng.   DOI   ScienceOn
12 Metabolic and kinetic analysis of poly(3-hydroxybutyrate) production by recombinant Escherichia coli. /
[ van Wegen, R. J.;S. Y. Lee;A. P. J. Middelberg ] / Biotechnol. Bioeng.   DOI   ScienceOn
13 Bacterial and other biological systems for polyester production. /
[ Steinbuchel, A.;B. Fuchtenbusch ] / Trends Biotechnol.   DOI   ScienceOn
14 Production of poly(3-hydroxybutyrate) from whey by fed-batch culture of recombinant Escherichia coli in a pilot-scale fermenter. /
[ Park, S. J.;J. P. Park;S. Y. Lee ] / Biotechnol. Lett.   DOI   ScienceOn