• Korean Journal of Medicinal Crop Science
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• v.2 no.3
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• pp.211-218
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• 1994

This experiment was conducted to obtain basic information for the establishment of in vitro initial culture system in Fritillaria thuubergii Miq. Methods of surface sterilization of scale segments as explant and effect of antibiotics added into the culture medium on contamination of explant and chilling treatment of mother bulb on bulblet formation were investigated. Portent of contamination of cultured scale segments was significantly higher in the outer scale segments which were unsuitable as initial culture explant than inner scale segments. Contamination of explants taken from inner scale of bulb was reduced by surface sterilizing explants in the solution of $4{\sim}5%$ sodium hypoclorite for $10{\sim}15$ mimutes. Addition of antibiotics such as kanamycin, vancomycia cefotaxim, agrirnycin and agreptomycin and dithane as fungicide and$lncyte^{tm}$ into MS medium was effective to reduce bateriological contamination, but did not work to control fungi. It had effective to delay the degree of contamination caused by fungi and bacteria haboring in cultured explants. Bulblet formation from cultured scale segments was promoted by dry storage for $2{\sim}4$ weeks or moisture storage of mother bulbs for $4{\sim}6$ weeks at $10^{\circ}C$ before excision of explants. Addition of kinetin into medium could not exerted for the bulblet formation from the scale segment of dry storaged bulb compared to control. But explant taken from 6 week moisture storaged bulb formed more than 10 bulblets per explant on the medium containing $3{\sim}5mg/L$ kinetin.

• Journal of Plant Biotechnology
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• v.3 no.2
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• pp.59-66
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• 2001

Photoautotrophic micropropagation systems do not include sugar in the culture media. This characteristic provides advantages to scale up the micropropagation systems comparing photomixotrophic micropropagation systems. A closed, large-scale photoautotrophic micro-propagation for transplant production system has been developed at Chiba University, Japan. New concepts and technologies were adapted to produce high quality transplants at minimum usage of resources, and as scheduled. Newly developed software for production management was used to enhance the efficiency of the transplant production system. Currently, virus-free transplants of sweetpotato (Ipomoea batatas (L.) Lam.) are vegetatively propagated and produced under sterilized conditions in this system. This system can also be used for production of transplants of any other species including horticultural and woody plants with a minimum of modification.

• International Journal of Advanced Culture Technology
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• v.5 no.1
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• pp.70-75
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• 2017

Recent development of IT industry including smart phones and communication technologies has resulted in rapid growth of Internet of Things (IoT) technology and relevant markets. The access to IoT is becoming easier thanks to the boards and IoT products, such as Arduino and Raspberry Pi. Large-scale business sites use IoT technology to manage inventories, but small-scale business sites do not. In the present study, We ported Linux-based Raspbian to Raspberry Pi, It utilizes web server communication to control the Arduino through the application We used a color sensor to figure out the kind of inventory. We also built a database using MySQL to store the data. We used raspberry pi to check whether the proposed system works and apply it to small-scale business.

• International Journal of Advanced Culture Technology
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• v.10 no.1
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• pp.97-101
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• 2022

The perceiving a calling scale is composed of three constructs: transcendent sermon, meaningful and prosocial aspects. Based on the multiple group confirmatory factor analysis from the structural equation model, we confirmed the measurement invariance between Christians and non-Christians at the scale level. Furthermore, to investigate the mean difference between Christians and non-Christians, we performed latent mean analysis. The results showed that the three-factor structure is equivalent across Christians and non-Christians, and further confirmed metric invariance and scalar invariance. Thus, the perceived calling scale is applicable to both Christians and non-Christians without biases. We also examined mean differences between the two groups. Christians demonstrated significantly higher scores on the three factors but no difference on the living a calling. This study provided meaningful information in understanding calling, and we discussed limitations and future directions.

• The International Journal of Costume Culture
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• v.13 no.2
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• pp.101-103
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• 2010

Liberalization may be described as the opening up of the economy to the outside world by relaxing government trade and economic policy restrictions to allow imports. In Kenya, this took place in the early 1990s and it had negative and positive effects on businesses manufacturing and retailing clothing and textiles. This case study narrates what has happened over the last 20 years to businesses in Nairobi, Kenya dealing in clothing and textiles. Some of the original businesses have closed down and been replaced by new ones. More small scale Kenyan businesses have been started dealing in clothing imports from the East and West selling both new and second hand clothes. There has been increased employment in the small scale clothing and textiles sector in garment making and retailing. All in all, liberalization has forced large and medium scale textile manufacture to change strategy so that they target selling to the export market more than to the local market.

• International Journal of Advanced Culture Technology
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• v.11 no.3
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• pp.247-252
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• 2023

The purpose of the study was to examine the mediating effect of kindergarten teachers' psychological burnout in the relationship between grit and teacher-child interactions. We conducted a cross-sectional study with 191 kindergarten teachers in South Korean. To measure the major variables, Grit scale [1], teacher-child interaction scale [2], and psychological burnout scale [3] were used. Hayes' PROCESS macro was used to test mediation effects of psychological burnout in the relationship between grit and teacher-child interactions. Indirect effects were tested using bootstrapped confidence intervals. As the results, kindergarten teachers' teacher-child interactions were found to have a significantly positive correlation with their grit but a significantly negative correlation with their psychological burnout. In addition, the relationship between grit and teacher-child interactions was mediated by psychological burnout.

• International Journal of Advanced Culture Technology
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• v.11 no.4
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• pp.163-168
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• 2023

The purpose of the study was to examine the mediating effect of early childhood teachers' professional development in the relationship between intrapersonal intelligence and teacher-child interactions. We conducted a cross-sectional study with 324 early childhood teachers working at kindergartens and child-care centers in South Korea. The empirical data using survey questionnaires were collected by administering the Adult Introspective Intelligence Scale, the Professional Development Scale for Early Childhood Teachers, and the Teacher-child Interactions Scale. SPSS and Hayes' PROCESS macro weres utilized. The results showed the statistically positive correlations between the major variable and the mediating effect of early childhood teachers' professional development in the relationship between intrapersonal intelligence and teacher-child interactions.

• Journal of The Korean Association For Science Education
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• v.33 no.2
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• pp.466-477
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• 2013

The purpose of this study is to explore the measurement of attitude towards science based on social constructivism and the application of this scale. The data were collected using questionnaires from 833 middle school students in the second grade. First, factor analysis were used to assess the construct validity of the scale of Reputation in Culture Border Crossings (R-CBC) developed by Krogh and Thomsen (2005). This finding indicated the construct validity of R-CBC scale based on social constructivism. Second, the correlation among two scales based on social constructivism and Test of Science-Related Attitudes (TOSRA) scale based on individual constructivism were investigated to examine the convergent validity and the discriminant validity. The R-CBC scale was correlated with Simpson-Troost Attitude Questionnaire (STAQ) scale but the R-CBC scale and TOSRA scale were not connected. Third, students' attitude toward science assessed by three forms of measurements were compared according to the choice of high school type. Students' attitude toward science measured by R-CBC scale and STAQ scale were different in three types of high school. This finding indicated that two scales based on social constructivism are useful measurements that can predict the choice of high school type.

• Journal of the Korean Dietetic Association
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• v.7 no.2
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• pp.144-152
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• 2001

This research reviews the characteristics of consumption oriented culture of post modern society and describes the food consumption culture based on the evaluative criteria pattern in eating in Ulsan Metropolitan City. The subjects consisted in 217 full-time housewives and 129 cooks. The evaluative criteria of eating scale with 4 components - the eating atmosphere factor, the preference factor, the quality factor and the table factor- is described and interpreted in the context of consumption oriented culture. 4 clusters were decided through cluster analysis; preference and quality pursuing type, high involvement type, consumerism culture pursuing type and low involvement type. Among socio-economic variables, the evaluative criteria pattern of eating was significantly associated with occupation. The results of factor analysis and cluster analysis proved that the full-time house wives and cooks in Ulsan Metropolitan city showed the propensity to consumption oriented culture in food consumption culture.

• Journal of Microbiology and Biotechnology
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• v.23 no.9
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• pp.1308-1321
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• 2013

The emergence of microcarrier technology has brought a renewed interest in anchorage-dependent cell culture for high-yield processes. Well-known in vaccine production, microcarrier culture also has potential for application in other fields. In this work, two types of microcarriers were evaluated for small-scale monoclonal antibody (mAb) production by CHO-K1 cells. Cultures (5 ml) of microporous Cytodex 3 and macroporous CultiSpher-S carriers were performed in vented conical tubes and subsequently scaled-up (20 ml) to shake-flasks, testing combinations of different culture conditions (cell concentration, microcarrier concentration, rocking methodology, rocking speed, and initial culture volume). Culture performance was evaluated by considering the mAb production and cell growth at the phases of initial adhesion and proliferation. The best culture performances were obtained with Cytodex 3, regarding cell proliferation (average $1.85{\pm}0.11{\times}10^6$ cells/ml against $0.60{\pm}0.08{\times}10^6$ cells/ml for CultiSpher-S), mAb production ($2.04{\pm}0.41{\mu}g/ml$ against $0.99{\pm}0.35{\mu}g/ml$ for CultiSpher-S), and culture longevity (30 days against 10-15 days for CultiSpher-S), probably due to the collagen-coated dextran matrix that potentiates adhesion and prevents detachment. The culture conditions of greater influence were rocking mechanism (Cytodex 3, pulse followed by continuous) and initial cell concentration (CultiSpher-S, $4{\times}10^5$ cells/ml). Microcarriers proved to be a viable and favorable alternative to standard adherent and suspended cultures for mAb production by CHO-K1 cells, with simple operation, easy scale-up, and significantly higher levels of mAb production. However, variations of microcarrier culture performance in different vessels reiterate the need for optimization at each step of the scale-up process.