• 한국약용작물학회지
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• 제20권4호
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• pp.217-221
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• 2012

This research was conducted to investigate the influence of various organic substrates on growth and yield of ginseng seedling grown organically in the closed plastic house. The pH and EC of substrates used for organically ginseng cultivation ranged 5.93~6.78 and 0.03~0.15 dS/m respectively. The concentrations $NH_4$-N and $NO_3$-N respectively was 14.01~68.63 mg/L, 5.60~58.83 mg/L. The average quantum of the closed plastic house was range from 10 to 16% of natural light. In July and August, the maximum temperature of the closed plastic house did not exceed 30 and the average temperature was maintained within 25 lower than the field because air conditioning ran. The PPV-1 and PPV-2 bed soil substrates produced higher stem length, stem diameter, shoot fresh weight and leaf area than those of conventional culture. In PPV-2 bed soil substrates, root fresh weight and root diameter was the highest. The root fresh weight of PPV-2 bed soil substrates in closed plastic house was maximum 25% heavier than the conventional cultivation. The results of this experiment will be utilized for making new substrate application for organic ginseng culture in the plastic house.

• The Plant Pathology Journal
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• 제35권5호
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• pp.425-436
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• 2019

Botrytis cinerea, a major phytopathogenic fungus, has been reported to infect more than 200 crop species worldwide, and it causes massive losses in yield. The aim of this study was to evaluate the inhibitory abilities and effects of Bacillus amyloliquefaciens RS-25, Bacillus licheniformis MG-4, Bacillus subtilis Z-14, and Bacillus subtilis Pnf-4 and their culture filtrates and extracts against the gray mold caused by B. cinerea on postharvest tomato, strawberry, and grapefruit. The results revealed that the cells of Z-14, culture filtrate of RS-25, and cells of Z-14 showed the strongest biocontrol activity against the gray mold on the strawberry, grape, and tomato fruit, respectively. All the strains produced volatile organic compounds (VOCs), and the VOCs of Pnf-4 displayed the highest inhibition values. Based on headspace solid-phase microextraction in combination with gas chromatography-mass spectrometry, esters accounted for the largest percentage of the VOCs produced by RS-25, MG-4, Z-14, and Pnf-4 (36.80%, 29.58%, 30.78%, and 36.26%, respectively). All the strains showed potent cellulase and protease activities, but no chitinase activity. RS-25, Z-14, and MG-4, but not Pnf-4, grew on chrome azurol S agar, and an orange halo was formed around the colonies. All the strains showed biofilm formation, fruit colonization, and lipopeptide production, which may be the main modes of action of the antagonists against B. cinerea on the fruit. This study provides the basis for developing natural biocontrol agents against the gray mold caused by B. cinerea on postharvest fruit.

• 생명과학회지
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• 제19권12호
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• pp.1764-1768
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• 2009

본 연구는 소의 과립막세포 배양상층액에서 스테로이드 호르몬의 농도와 생쥐 배의 체외 발달에 미치는 효과를 검토하기 위하여 수행되었다. 소의 성숙난포(직경 6~15 mm)와 미성숙 난포(직경 2~5 mm)로부터 과립막세포를 각각 분리하여 15% FCS가 첨가된 Ham's F-10 배양약에서 16일간 배양하였다. 과립막세포들은 쉽게 단층배엽을 형성 하였으며, 배양과정 중 비슷한 성장패턴을 나타내었다. 또한 성숙 과립막세포와 미성숙 과립막세포 간에 형태적인 차이가 없었다. 과립막세포 배양 중 progesterone과 estradiol 생산이 활발하게 이루어졌으며, progesterone은 배양후기에, estradiol은 배양초기에 분비량이 높았다. 성숙 과립막세포와 미성숙 과립막세포에서 내분비 양상은 비슷하였다. 생쥐배가 상실배, 배반포 및 부화배반포 단계로 체외발달된 비율은 Ham'F-10배양액(86.7%, 41.7%, 13.3%)에 비하여 성숙 과립막세포 배양상층액(92.7%, 78.1%, 34.5%)과 미성숙 과립막세포 배양상층액(96.4%, 78.5%, 26.8%)에서 유의적으로 높았다(p<0.05). 그러나 배의 발달에 대하여 성숙 과립막세포배양상층액과 미성숙 과립막세포 배양상층액 간에는 차이가 없었다.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2007년도 제48회 학술심포지움 및 추계국제학술대회
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• pp.45.1-45.1
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• 2007

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• 한국환경농학회지
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• 제26권4호
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• pp.332-336
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• 2007

Pseudomonas stutzeri strain KCCM 34719 was used in this experiment to determine the effects of increasing Pb(II) concentrations on its growth rate. To obtain optimum growth conditions, strain KCCM 34719 was cultivated in nutrient broth under various conditions, such as temperature, pH, and NaCl concentration. Optimal conditions for cell growth were $30^{\circ}C$ of temperature, 8.0 of pH, and 3% of NaCl concentration, respectively. Growth response of bacterial cell to Pb(II) showed tolerance to concentrations ranging from 10 to 100 mg ${\ell}^{-1}$ in liquid culture, following a growth pattern similar to the control. Growth rate was greatly inhibited at 200 mg ${\ell}^{-1}$ of Pb(II).

• Journal of Hospice and Palliative Care
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• 제1권1호
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• pp.1-5
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• 1998

Grief is the ordinarily self-limited complex of symptoms and processes that constitute the acute reaction to a significant loss. And it is the reaction of the dying as well as the bereaved. Every culture has had its own ways of grief and mourning. The definition of healthy grief and mourning, in terms of both emotional expression and the length of time it should continue, mostly depend upon the type of culture as well as the type of religion. So the manner of grief and mourning greatly differs from culture to culture. In the most of the Asian countries, influenced by Confucianism, Taoism, and Buddhism, death is traditionally considered the most significant life cycle transition. In Chinese culture, many rituals have evolved to help family members deal with their loss, over the past five thousand years. Confucianism taught the virtues of filial piety and righteousness. These rules and many customs added since the time of Cofucius, have been loyally followed and practiced by many Asian people. However, Buddhists have different ideas. They believe in karma and reincarnation and in predetermination of one's present life by good or bad deeds in the present life and past lives. Display of uncontrollable emotion is not encouraged. Continuity of family relations after death is very important. The ancient practice of the ancestor worship is still followed in many Asian households. Many Buddhist do not practice ancestor worship; family members honor the deceased by placing a memorial plate in the temple for continued chanting purposes. The mourning rituals have been dramatically curtailed in the past 50 years. For example, political, social and economic forces have shaped the current mourning practices of Chinese in different countries. There are many clinical implications in helping Asian to deal better with the emotional strains of the experience of loss. The therapiest must respect the cultural framework through which the client perceives family losses.

• 한국조리학회지
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• 제12권1호
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• pp.157-172
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• 2006

Food Culture is formed in a unique culture heritage in some areas harmonized with social factors mixed with geographical conditions, natural environment, culture and religion. The more the life skill develops highly, the more people focus on improvement of the life skill in order to realize a delightful and convenient life, and plan an increase of production. Recently food-service companies have changed very quickly with economic environment. Such change will be continued and its speed will be more faster than now. Many food-service companies will disappear for weak competition, if they don’t manage adequately in such a case. Therefore, food-service companies have to search for an efficient, unique and creative management strategy. In such a viewpoint, Kyunggi-do designated five local cities as a model of Food Culture Streets greeting Welcoming Year of Kyunggi-do in 2005. The purpose of this study is to serve the healthy food to visitors visiting the Food Culture Streets and to do public relations about the excellence of their traditional food. In addition, this study includes improving tastes and qualities of food through education about cooking skills, management strategies and the best service with unique and creative menu development. Accordingly, we need to analyze the factors continuously on the business of food culture streets, and intend to use them as a database for high quality education and activity of food service industry in the future.

• 한국버섯학회지
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• 제21권4호
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• pp.195-199
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• 2023

The fungus Alternaria alternata, responsible for causing brown to black spotting on numerous fruits and vegetables globally, was identified in 2022 as the causative pathogen of brown spot disease in potatoes in Korea. In pursuing potential inhibitors against A. alternata growth, we evaluated 15 mushroom culture filtrates: eight from Trametes spp. and seven from Polyporus spp., known for their antibacterial and anticancer properties. Antifungal activity was assessed by exposing each filtrate to A. alternata on a paper disc. Four filtrates displayed inhibitory action against the fungus, albeit with mild effects. Our findings highlight the potential of Trametes and Polyporus fungi as emerging antifungal candidates, offering promise in preventing potato brown spots.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2006년도 추계학술대회 및 정기총회
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• pp.7-9
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• 2006

• 한국자원식물학회지
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• 제19권2호
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• pp.218-222
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• 2006

재배시기, 시설형태 그리고 시설내 계배위치에 따른 가상환경의 차이와 이에 따른 양액계배 오이의 생장 반응을 알아보기 위하여 여름재배와 억제재배, 1-2W형 하우스와 무기둥 하우스에 양액계배를 통해 오이를 패배한 후 시설내 부위별 기온, 상대습도, 일사량과 생장해석을 통해 얻어진 생리적 형질들을 조사하여 비교한 결과는 다음과 같다. 하우스 내부의 평균기온이나 평균상대습도는 하우스 형태간 유의한 차이가 없었으나 일사량은 1-2W형 하우스가 무기둥 하우스에 비해 유의하게 높았다. 일누적일사량은 1-2W형에서는 남서쪽이 무기둥 하우스에서는 북서쪽이 대체로 높았다. 초장과 엽수는 억제재배보다 여름재배에서 높게 나타났으며 엽면적은 억제재배에서 많았다. 상대생장율은 순동화율 및 엽면적비율과 고도로 유의한 정의 상관관계를 보였으며 상대생장율에 대한 기여도는 순동화율이 엽면적비율보다 높은 것으로 나타났다. 작물생장율은 엽면적지수와 고도로 유의한 정의 상관을 보여 엽면적지수의 증대가 단위면적당 생산량의 증대에 크게 영향하는 것으로 나타났다. 전 생육기간의 일평균기온는 상대생장을 및 순동화율과 고도로 유의한 정의 상관관계가 있었다. 억제재배에서는 전 생육기간의 누적일사량과 상대생장을 및 순동화율과 유의한 정의 상관관계가 있었다.