• The Journal of the Korean Society for Microbiology
• /
• v.11 no.1
• /
• pp.79-85
• /
• 1976

There are many of anaerobic culture methods and equipments for isolation and cultivation of anaerabic bacteria, but most of these methods are used without pre-reduced media. Gas-jet method is a recommend. able method for the culture of anaerobes, resently developed. Bacteriological studies were experimented of liver abscess of cattle by the use of gas. jet method. The results were summarised as follows; 1. Gas-jet method for anaerobic culture are expedient for the making of pre-reduced media, maintaining of oxygen free condition in the culture tube, picking of bacteria from colony and colony counting etc. 2. A 121 strains of facultative anaerobic and anaerobic bacteria were isolated from liver abscess of 27 head of cattle, and the isolated anaerobic bacteria were as follows. Peptostreptococcus spp. 7 strains Acid aminococcus fermentans 1 Veillonella spp. 1 Bacterioides spp. 6 Bifidobacterium spp. 4 Arachinia propionica 2 Lactobacillus spp. 4 Propionibacterium acnes 1 3. Liver abscess were infected with many of bacteria, about $10^3-10^9$ numbers per gram of abcessed tissue. Almost of abscess were mixed infection of various bacterial species rather than simple species.

• Korean Journal of Agricultural Science
• /
• v.38 no.4
• /
• pp.613-618
• /
• 2011

To develop clubroot resistant Chinese cabbage inbreds, IT 033820, a clubroot resistant turnip, was cross pollinated with a Chinese cabbage inbred of BP 079. From 2005, conventional breeding and microspore culture method performed using these F1 plants as parental materials. In 2007, conventional breeding method resulted in 21 F3 inbreds. After inoculation of clubroot race 4, one inbred showing 83% resistant was selected and registered as 'onkyo 20036ho' in 2008. From 2005, we scanned hybrid cultivars using micro spore culture and developed many doubled haploid (DH) lines in Chinese cabbage. Using Chinese cultivar of 'oong-baek 2ho' we developed 26 DH inbreds in 2007. After inoculation of clubroot race 4, one DH inbred showing 77% resistant and yellow inner leaf color was selected and registered as 'onkyo 20034'in 2008. We found conventional breeding method was effective using introduced germplasm showing low germination. However, when using hybrid cultivar as starting material, microspore culture method was powerful for developing various inbred in short time.

• Iberoamérica
• /
• v.12 no.1
• /
• pp.407-439
• /
• 2010

Growth of researches addressing cross-culture in international business is exponential. This article reviews the extant researches around the national culture and describes the various conceptualization of culture through discussion of some of popular models of national culture. This article presented some of the most important issues in international business surrounding globalization, especially convergence and divergence of cultures and cultural changes. Global rapid changes in international business environment request the reconsideration of the assumption of cultural stability and the simple view of culture, which tends to examine the static influence of a few cultural factors in isolation form other cultural factors and contextual elements. This paper identifies a valid cultural grouping and proposes the following typology of the possible methodologies in international business; Ethnological description, Use of proxies, Direct values inference, and Indirect values inference. Rather than selecting a single methodology, it appears to be more appropriate to use multi-method in the cross-cultural international business research. It has been shown that cultural change is intertwined with socioeconomic-institutional variables, and that these variables may also add to determine culture contemporarily. This paper also explained the dynamics of culture as multi-level, multi-layer constructs. According to this model, we may understand how the dynamic nature of culture conveys the top-down-bottom-up processes where one cultural level affects changes in other level of culture.

• Journal of Korean Academy of Nursing Administration
• /
• v.12 no.2
• /
• pp.265-275
• /
• 2006

Purpose: The purpose of this study is to identify and to investigate the nursing organizational culture. Method: A descriptive correlational study was conducted with convenience sample of 448 staff nurses who were working for six general hospitals located in Seoul, Province of Gyeonggi and Province of Chung-cheong. The period of data collection took over 5 months between August and December. Organization Culture Questionnaire developed by Lee (2001) were used to measure nursing organizational culture. Collected data were analysed on SPSS Win 12.0. Result: The meta culture of the nursing organizations was the conservative culture. The cultural pattern of nursing organizations was classified into three patterns which were conservative-dominant culture, symmetric culture and innovative-task culture. Conclusions: The meta culture of the nursing organizations was the conservative culture. But conservative culture decreased nurses' outcomes. Therefore the nurse manager should develop a symmetric organizational culture including four types of culture than dominant culture of one type.

• Journal of Microbiology and Biotechnology
• /
• v.21 no.1
• /
• pp.100-108
• /
• 2011

A method for the rapid detection and quantification of Newcastle disease virus (NDV) produced in an animal cell culture-based production system was developed to enhance the speed of the NDV vaccine manufacturing process. A SYBR Green I-based real-time RT-PCR was designed with a conventional, inexpensive RT-PCR kit targeting the F gene of the NDV LaSota strain. The method developed in this study was validated for specificity, accuracy, precision, linearity, limit of detection (LOD), limit of quantification (LOQ), and robustness. The validation results satisfied the predetermined acceptance criteria. The validated method was used to quantify virus samples produced in an animal cell culture-based production system. The method was able to quantify the NDV samples from mid- or late-production phases, but not effective on samples from the early-production phase. For comparison with other quantifiable methods, immunoblotting, plaque assay, and tissue culture infectious dose 50 ($TCID_{50}$) assay were also performed with the NDV samples. The results demonstrated that the real-time RT-PCR method is suitable for the rapid quantification of virus particles produced in an animal cell-culture-based production system irrespective of viral infectivity.

• The International Journal of Costume Culture
• /
• v.4 no.3
• /
• pp.195-202
• /
• 2001

Re purpose of this study is to indicate the framework to analyze the contemporary fashion phenomena taking on aspect as above by grafting the mass culture theory onto the study for fashion phenomena. The contemporary culture phenomena look like a same thing apparently, but various individual characters appeared in them. And all cultures are mixed in the name of mass culture, but the subordinate concepts which can classify the culture are still in existence. And this equally appear in the contemporary fashion, one of culture phenomena. The concept of the contemporary mass culture can be explained with the taste culture, Cans' theory indicated, divided into highbrow culture, middlebrow culture, and lowbrow culture. And the phenomena of the contemporary mass culture also can be explained with globalization that came down to homogenization, regionalization hybridization.

• Journal of Korean Society of Industrial and Systems Engineering
• /
• v.18 no.33
• /
• pp.55-61
• /
• 1995

The corporate culture contributes much to the approach to the management problems as well as to the view to see the problems in the light of the multiplying-enterprise environment. In comparision with the large size corporates, the small-medium size corporates under various disadvantage conditions increase to the high achievement through the development of corporate culture, though the period and method to root in the corporate culture differ from one another depending on the situations. The purpose of this paper is to propose the owner's intention and efforts, the development of the corporate culture helpful for the long-run achievement, the cultural network and the member's role which prove to be effective method to root in the corporate culture.

• Journal of Environmental Health Sciences
• /
• v.28 no.2
• /
• pp.193-202
• /
• 2002

The polymerase chain reaction(PCR) of target lacZ and uidA genes were used to detect total coliform and Escherichia coli for determining water quality, respectively. Of 109 spring waters, coliform were detected from 38 spring waters by lacZ PCR method but 21 spring waters by culture method accepted by the Ministry of Environment for water quality monitoring. The lacz PCR method gave the results statistically equivalent to those of the culture method(kappa=0.62, McNemar=17.00). The uidA PCR method gave the same results to those of the culture method. The sensitivity and specificity of coliform and E. coli by PCR method were 100% and 80.7%, respectively. Therefore, PCR can be used for the rapid identification of Escherichia coli and coliform in potable water using uidA and lacZ.

• Proceedings of the Costume Culture Conference
• /
• 2000.09a
• /
• pp.82-83
• /
• 2000

• English & American cultural studies
• /
• v.9 no.1
• /
• pp.1-25
• /
• 2009

This paper attempts to illuminate the significance of the myth or mythical method used in The Waste Land, which Eliot adapted from Jessie L. Weston's From Rituals to Romance and Sir James Frazer's Golden Bough. While he was composing a modern epic, James Joyce's Ulysses and Igor Stravinsky's Le Sacre du Printemps made him sure that the mythical method would be the best way to make the non-relational and chaotic modern world into a work of art. Although he accepted F. H. Bradley's epistemology that one's actual experience is non-relational, he strongly put an emphasis on 'the unified sensibility' in John Donne's poetry with which a poet changes all the dissociated material into art. He also found another effective method to give the chaotic experiences an order, and to make them modern art: the mythical method in his contemporary anthropology. With the mythical method he incorporated the various barren, horrible and ugly aspects of modern world into a new unity in The Waste Land. In addition, he embraced his contemporary anthropological theory that a primitive life described in myths is a culture just different from modern culture, and heartily employed some aspects of primitive culture to make modern poetry as well as modern culture rich and exuberant.