• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.4
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• pp.275-287
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• 2000

Fermentation strategies for recombinant protein production in Pichia pastoris have been investigated and are reviewed here. Characteristics of the expression system, such as phenotypes and carbon utilization, are summarized. Recently reported results such as growth model establishment, app58lication of a methanol sensor, optimization of substrate feeding strategy, DOstat controller design, mixed feed technology, and perfusion and continuous culture are discussed in detail.

• The Journal of the Convergence on Culture Technology
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• v.1 no.3
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• pp.69-75
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• 2015

In recent society, great and small accidents have happened frequently, so the demands for safety have increased. As numerical increase of tourists who use the amusement facilities, many accidents owing to amusement facility has arisen. The goal of this study is that suggests the improvement plans for the safety management system of amusement facilities. In order to achieve the goal of study, legislations, industrial scale, accident situation, and others relating to amusement facility business were considered in a lot of ways. From a series of study results, improvement plans are suggested for safety inspection, accident compensation insurance, accident investigation, and others relating to amusement facility business.

• Journal of Korean Society of Rural Planning
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• v.19 no.3
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• pp.75-88
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• 2013

This study aims at reviewing satisfaction on facility modernization project of traditional market by case study on integration & move method of 2 markets (Seongbuk 5 day market & Geumgye daily market). Following this study, it asked 182 merchants & customers at Naju Moksagoeul Market about satisfaction and performance on integration & move and the facility modernization project of traditional markets. According to results of the survey, not only merchants but also customers are satisfied with the modernization project. But in detail, valuations between 5 days market and daily market, and between merchants and customers are different. For example, merchants regard as negative, but customers as positive about move of the markets from city to outside. About half of respondents answer that number of market visits and sales volume after move of the markets are higher than before. More than 80% of respondents answer that culture & tourism market programs are helpful to the market revitalization.

• Journal of the Korea Convergence Society
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• v.8 no.11
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• pp.299-306
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• 2017

In this paper, the development of differentiated programs for visitors as well as those for teaching, ways on how to make these halls a culture complex that can support diverse cultural activities for citizens, and strategies to increase their utilization for the public as an facility open to the community, given the trend that intangible cultural heritage teaching halls are getting bigger and complex. The intangible cultural heritage teaching halls of it is need to make improvements to the facility for their activation. Therefore In this paper as a collected information on their establishment and administration to find ways for their activation from a user perspective by visiting three intangible cultural heritage teaching halls except Seoul and large cities of metropolitan areas. We expect that findings from the study will provide a basis to set directions to make intangible cultural heritage teaching halls a culture complex facility.

• Journal of Microbiology and Biotechnology
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• v.32 no.2
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• pp.238-247
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• 2022

Since the skin covers most surfaces of the body, it is susceptible to damage, which can be fatal depending on the degree of injury to the skin because it defends against external attack and protects internal structures. Various types of artificial skin are being studied for transplantation to repair damaged skin, and recently, the production of replaceable skin using three-dimensional (3D) bioprinting technology has also been investigated. In this study, skin tissue was produced using a 3D bioprinter with human skin cell lines and cells extracted from mouse skin, and the printing conditions were optimized. Gelatin was used as a bioink, and fibrinogen and alginate were used for tissue hardening after printing. Printed skin tissue maintained a survival rate of 90% or more when cultured for 14 days. Culture conditions were established using 8 mM calcium chloride treatment and the skin tissue was exposed to air to optimize epidermal cell differentiation. The skin tissue was cultured for 14 days after differentiation induction by this optimized culture method, and immunofluorescent staining was performed using epidermal cell differentiation markers to investigate whether the epidermal cells had differentiated. After differentiation, loricrin, which is normally found in terminally differentiated epidermal cells, was observed in the cells at the tip of the epidermal layer, and cytokeratin 14 was expressed in the lower cells of the epidermis layer. Collectively, this study may provide optimized conditions for bioprinting and keratinization for three-dimensional skin production.

• The Journal of the Korea Contents Association
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• v.13 no.6
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• pp.507-517
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• 2013

Welfare desire has been diversified according to the increase of economic level and rapid social change. The desire for comprehensive service on culture, medical, mental and information level has appeared. Accordingly, it is necessary to establish an integrated community mixed-support facility, a space of accepting administration, welfare, leisure, culture and informatization request in a small local community in the future. The community mixed-support facility is a meeting place of local resident for information change, various basic service and ultimately for activation of community in order to contribute to regeneration and activation of existing urban core. Thus, this study figured out general status of community mixed-support facility and physical mixed type and analyzed spatial characteristics. Current community mixed-support facility has insufficient program and space to participate in by local residents and to accommodate mixed function. The future planned community mixed-support facility must secure multiple opportunities for various types of life by returning time and cost to user through the introduction of creative mixed concept. While reflecting unique local characteristics, and considering diversity of users, it needs to make mixed facility to cope with social changes. This study is limited in that the study reached a conclusion by survey and analysis only. However, it is expected that the result can be used as basic research data for the planning of community mixed-support facility to cope with living conditions of local residents or realistic requirements.

• The Research Journal of the Costume Culture
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• v.17 no.3
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• pp.367-382
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• 2009

The aim of the current study was to investigate the impact of store attribute satisfaction on intentions of revisit and recommendation among clothing consumers. The data were collected from 319 consumers through survey and frequency analysis, reliability analysis, factor analysis, and multiple regression analysis were used to obtain results. The findings were as follows: 1. From factor analysis, seven factors were distracted: Fact 1(brand and price), Fact 2(store's facility and environment), Fact 3(product), Fact 4(transportation convenience and access), Fact 5(selling and advertisement), Fact 6(store's atmosphere), and Fact 7(salesman's service). 2. Four factors had statistically significant influence on overall satisfaction of clothing consumers. The most influential factor was Fact 2(store's facility and environment) and Fact 5(selling and advertisement), Fact 1(brand and price), and Fact 4(transportation convenience and access) showed their effects on overall satisfaction in an hierarchical rank-order following Fact 2. 3. Four factors such as Fact 2(store's facility and environment), Fact 1(brand and price), Fact 4(transportation convenience and access) and Fact 5(selling and advertisement) in an hierarchical rank-order from Fact 1 had statistically significant impact on intentions of revisit. 4. Six factors such as Fact 1(brand and price), Fact 2(store's facility and environment), Fact 3(product), Fact 5(selling and advertisement), Fact 6(store's atmosphere), and Fact 7(salesman's service) in an hierarchical rank-order from Fact 1 had statistically significant influence on the intention of recommendation. 5. The results further showed that among seven factors, Fact 1(brand and price), 'Fact 2(store's facility and environment), and Fact 5(selling and advertisement) had impact on both the intention of revisit and the intention of recommendation.

• The Journal of the Convergence on Culture Technology
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• v.2 no.3
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• pp.1-7
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• 2016

Infection of the disease that are propagated through a dead body of management and transportation must be very careful. Risk of infection that can occur between the deceased body and funeral directors, guideline of the preparation room and infection route of infection source has been studies in Korea. But the funeral director's working circumstances and infection blocking facility of the funeral home has not been studied in Korea. So we are investigated about the state of the working circumstances and Infection blocking facility within Metropolitan, Chungcheong and Gyeongsang regions in Korea. The result is as follows. The funeral directors have shown that there is difficult to get safe protection from infection. The personal hygiene of funeral directors and the infection blocking facility should be strengthened institutionally. We are thought to require more in-depth research about the working circumstances of funeral director and the infection blocking facility.

• KIEAE Journal
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• v.16 no.4
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• pp.47-54
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• 2016

Purpose: Grafting new hanok-style application technology on child daycare facility among public buildings can be an alternative that provides infant facility, which is placed at the center of social attention and interest, with pleasant nursing environment. Besides, it is expected to have a great impact on infants' and young children's emotion by helping them recognize traditional culture at early stage and furthermore can be used for villagers to experience differentiated traditional culture. Method: To investigate the recent construction of HeungCheon/SuRak/GeumGwa's Hanok child daycare facility recognizing for architectural planning characteristics in common tendency Hanok that presents a physical improvement plan for preparing the continued competitiveness. Also attention to the creative expression of the traditional spaces about the natural environment are implemented in Nurseries and we propose the possibilities that can be realized in the future Hanok child daycare facility. Result: The combination of modern materials and modern construction technology of New-hanok method with improved ability has improved the shortcomings of traditional architecture and increase the ease and safety of traditional architecture succeed the suggest direction of improvement modern society. Therefore, Hanok child daycare facility is preparing adequate capability to solve regional polarization and social care issues.

• Korean Institute of Interior Design Journal
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• v.15 no.2 s.55
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• pp.99-107
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• 2006

People with visual impairments are difficult to go to education facility by themselves, because of their limited visual ability, orientation, and usability field, so they prefer dormitories. Those dormitories playa role in living space, education, communication and culture function. Therefore those facilities planned more user centered design, since they are influenced by user actability and their values. User centered designs that match between the user and space are understood as well as their characteristics and behavior. Especially for understanding their activity, observation is conducted about their behavior in dormitories. Thus the purpose of this study is suggested to be a basic source that planning about residential facility for people with visual impairments through interview and observation investigation by analysis that they resident in space motion and performance.