• Title/Summary/Keyword: culture content

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In Vitro Sugar Accumulation in Juice Sacs of 'Shiranuhi' Mandarin

  • Moon, Doo-Gyung;Han, Sung-Gap;Joa, Jae-Ho;Kim, Chun-Hwan;Seong, Ki-Cheol
    • Horticultural Science & Technology
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    • v.31 no.3
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    • pp.269-274
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    • 2013
  • To further our understanding of sugar accumulation in 'Shiranuhi' mandarin [(C. unshiu ${\times}$ C. sinensis) ${\times}$ C. reticulate], we investigated the patterns of sugar uptake in juice sacs exposed to different concentrations of sucrose, fructose and glucose in vitro. Data was also collected on the change in weight and shape of the in vitro juice sacs over time. Soluble solids content, sugar content and acidity content were highest at 20% sucrose, fructose and glucose solution content; while fructose content was highest at 5% sucrose concentration. Furthermore, the juice sac's fresh weight was highest at 5% sucrose and lowest at 20% fructose content. The shape of the juice sacs also differed in different sugar concentration and type. Overall, sucrose, fructose and glucose content in juice sacs increased with the sugar concentration. These results suggest that sugar translocation into juice sacs is actively induced by high sugar concentration in the medium. Thus, it can be concluded that sugar and acid accumulation in juice sacs increased with sugar concentration in vitro culture.

Quality Characteristics of Processed Mushroom Products Using Pleurotus ostreatus, Agaricus bisporus and Flammulina velutipes

  • Chang, Min-Sun;Kim, Myoung-Sook;Cho, Sun-Duk;Jhune, Chang-Sung;Cho, Weon-Dae;Yoo, Young-Bok;Kim, Gun-Hee
    • Food Quality and Culture
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    • v.3 no.1
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    • pp.6-10
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    • 2009
  • This study examined the effects of adding Pleurotus ostreatus, Agaricus bisporus and Flammulina velutipes on the quality characteristics of cookies and multiple purposes of wheat powders. The water content, crude protein content, mineral content, color value and sensory evaluation of mushroom cookies and multiple purposes of wheat powders were measured. In the case of cookies, the water content was 1.10-1.24% and crude protein content was 8.20-9.80%. General preferences, such as appearance, color, taste and texture, increased when mushroom was added to the cookies, especially flavor, which showed a much higher preference. In the studies where mushroom was added to wheat powders, the water content was 0.57-0.92% and crude protein content was 11.90-12.80%. The addition of mushroom to both of the cookies and multiple purposes of wheat powders resulted in a remarkable increase in the mineral content, especially Fe, K. In addition, an increase in the amount of added mushrooms resulted in an increase in the hunter L and a values; however, this also resulted in a decrease in the b value.

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Quality Characteristics of Muffin with Added Grape Powder (포도분말을 첨가한 머핀의 품질특성)

  • Jeong, Hyun-Chul;Jeon, Hyeon-Mo
    • Journal of the Korean Society of Food Culture
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    • v.31 no.5
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    • pp.498-505
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    • 2016
  • This study investigated grape powder substituted for wheat flour in muffin recipes with the amounts of 0, 5, 10, 15, and 20%. Grape powder consisted of 6.76% of moisture content, 4.63% of crude protein, 4.82% of crude fat and 4.16% of crude ash. DPPH radical scavenging activity is 68.79%, total phenol content is 205.79. Specific loaf volume have decreased as the grape powder content increased. Baking loss have increased as the grape powder content increased. Moisture and brix have increased as the grape powder content increased. pH have increased as the grape powder content decreased. Chromaticity measurement result showed that the 'L' and 'b' value of muffins decreased as grape powder content increased, whereas 'a' value increased. The texture measurement result showed that the hardness, gumminess and chewiness of muffins increased as the grape powder content increased, whereas springiness decreased. Cohesiveness did not show any considerable differences between muffins. Overall preference scores showed high overall acceptability for the muffins made with 10% grape powder.

Effect of Levulinic Acid on the Production of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Ralstonia eutropha KHB-8862

  • Chung, Sun-Ho;Park, Gang-Guk;Kim, Hyung-Woo;Rhee, Young-Ha
    • Journal of Microbiology
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    • v.39 no.1
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    • pp.79-82
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    • 2001
  • The influence of levulinic acid (LA) on the production of copolyester consisting of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) by Ralstonia eutropha was investigated. Addition of LA into the culture medium greatly increased the molar fraction of 3HV in the copolyester, indicating that LA can be utilized as a precursor of 3HV. In shake flask culture, the 3HV content in the copolyester increased from 7 to 75 mol% by adding 0.5 to 4.0 g/L LA to the medium containing fructose syrup as a main carbon source. A maximal copolyester concentration of 3.6 g/L (69% of dry cell weight) was achieved with a 3HV content of 40 mo1% in a jar fermentor culture containing 4.0 g/L of LA. When LA (total concentration, 4 g/L) was added repeatedly into a fermentor culture to maintain its concentration at a low level, the copolyester content and the 3HV yield from LA reached up to 85% of dry cell weight and 5.0 g/g, respectively, which were significantly higher than those when the same concentration of the LA was supplied al1 at once. The present results indicated that LA is more effective than propionate or valerate as a cosubstrate fur the production of copolyesters with varying molar fractions of 3HV by R. eutropha.

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Effects of Deep Seawater on the Growth of a Green Alga, Ulva sp.(Ulvophyceae, Chlorophyta)

  • Matsuyama, Kazuyo;Serisawa, Yukihiko;Nakashima, Toshimitsu
    • ALGAE
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    • v.18 no.2
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    • pp.129-134
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    • 2003
  • In order to examine the effects of deep seawater (mesopelagic water in the broad sense) on the growth of macroalgae, the growth and nutrient uptake (nitrate and phosphate) of Ulva sp. (Ulvophyceae, Chlorophyta) were investigated by cultivation in deep seawater (taken from 687 m depth at Yaizu, central Japan, in August 2001), surface seawater (taken from 24 m depth), and a combination of the two. Culture experiments were carried out in a continuous water supply system and an intermittent water supply system, in which aerated 500-mL flasks with 4 discs of Ulva sp. (cut sections of ca. 2 $cm_2$) were cultured at 20$^{\circ}C$ water temperature, 100 $\mu$mol photons $m^{-2}{\cdot}s^{-1}$ light intensity, and a 14:10 light:dark cycle. Nutrient uptake by Ulva sp. was high in all seawater media in both culture systems. The frond area, dry weight, chlorophyll a content, dry weight per unit area, and chlorophyll a content per unit area of Ulva sp. at the end of the experimental period were the highest in deep seawater and the lowest in surface seawater in both culture systems. These values, except for dry weight per unit area and chlorophyll a content per unit area, for each seawater media in the intermittent water supply system were higher than those in the continuous water supply system. We conclude that not only deep seawater as the culture medium but also the seawater supply system is important for effective cultivation of macroalgae.

Content Analysis of Patient Safety Culture in Nursing Homes (노인요양시설의 환자안전문화 인식에 관한 귀납적 내용분석)

  • Yoon, Sook-Hee;Wu, XiangLian
    • Journal of Korean Academy of Nursing Administration
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    • v.19 no.1
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    • pp.118-127
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    • 2013
  • Purpose: The purpose of this study was to identify experiences of incidents and to explore the perceptions of Patient Safety Culture between two groups using nursing homes in Korea; employees and patients and their families. Methods: In 2010 in-depth interviews were used to collect data from 56 participants (38 employees, and 18 patients and family members). The data were analyzed using inductive content analysis. Results: The analysis scheme resulting from employees' data consisted of 7 categories and 22 subcategories, after 216 significant statements were analyzed and categorized. The 7 categories were education and training (24.5%), working attitude (23.6%), organizational system (19.0%), job satisfaction (18.5%), institutional environment (6.5%), manager leadership (4.2%), and work climate (4.7%). The analysis scheme resulting from patient and family data consisted of 6 categories and 7 subcategories after 24 significant statements were analyzed and categorized. Education and training among categories of employees were excluded. Conclusion: These findings indicate that an evaluation tool for patient safety culture should be developed for nursing homes in Korea.

Fraud Risk Management Model: A Content Analysis Approach

  • MADAH MARZUKI, Marziana;NIK ABDUL MAJID, Wan Zurina;AZIS, Nur Kamaliah;ROSMAN, Romzie;HAJI ABDULATIFF, Nik Kamaruzaman
    • The Journal of Asian Finance, Economics and Business
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    • v.7 no.10
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    • pp.717-728
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    • 2020
  • The objective of this study is to explore the whole process of fraud risk management strategies that should be implemented by the organizations. Secondly, this study discusses the governance issues that arise at each stage of the process. For the purpose of this study, a content analysis of previous literatures is used as a technique for gathering data. This process usually involves codifying qualitative and quantitative information into pre-defined categories in order to derive patterns in the presentation and reporting of information. Based on our content analysis, we found that the fraud risk management process should be made of at least five stages which are inculcating the culture of managing risks in an organization, identifying the risks, evaluating the risks, determining preventive actions and implementing and reviewing stages. Our extended analysis of the fraud risk management process finds that a lot of governance issues arise in the fraud risk management process that should be solved by regulators and companies in order to ensure that fraud risk management process is embedded as corporate culture, not merely as a process. Among them are how to create the risk culture in an organization and whether auditors and risk management committees identify risks from each available source.

Growth Responses of Petunia as Affected by Several Pollutants in Vitro (Petunia 조직배양시 몇가지 공해물질이 생장반응에 미치는 영향)

  • 백기엽;최성렬;이재욱;최주견
    • Journal of Plant Biology
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    • v.27 no.3
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    • pp.139-147
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    • 1984
  • This in vitro study was employed to clarify the effects of several pollutants i.e. $SO_2$, fluoride, cadmium(Cd), aluminum(Al) and NaCl, on the organogenesis and growth responses of shoot-tip, stem and multiple-buds segments derived from hypocotyl or cotyledon culture of petunia seedlings. ${Na_2}{SO_3}$levels of more than 200$\mu{g}$/ml had significantly reduced organogenesis, growth, and chlorophyll content. The injuries caused by ${Na_2}{SO_3}$, concentration of more than 400$\mu{g}$/ml were alleviated by increasing hydrogenion concentration of medium, indicating some relationship between two factors. Organogenesis was not affected by the fluoride concentration up to 100ppm in the media, but the growth and chlorophyll content were greatly reduced by the fluoride. The effect of Cd depended on the explant sources used for the culture; 1.0ppm was effective for fresh weight increase in shoot tip culture, and 3.0ppm in stem segments culture. Organogenesis and growth were greatly reduced by more than 10.0 Cd treatment. Growth and formation of shoots were better with Na conc. of 0.3% compared to control, but those of roots were inhibited. Na concentration goes over 1.0%, organogenesis and subsequent growth were inhibited, and chlorophyll synthesis was drastically reduced. Chlorophyll content was increased on the medium supplemented with Al 50$\mu{g}$/ml compared to control. However the formation and growth of shoots were greatly inhibited with more than 400$\mu{g}$/ml and roots were not produced at all.

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Analysis of the Contents of Hanbok in the 「Home Life and Safety」 section of the High School Technical Family Textbook: Content Analysis and Text Mining Techniques are utilized (고등학교 기술·가정 교과서 「가정생활과 안전」 영역의 한복 내용 분석)

  • Shim, Joon Young;Baek, Min Kyung
    • Human Ecology Research
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    • v.59 no.2
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    • pp.261-273
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    • 2021
  • This study is not just a meaning of costume but a function of culture and includes addresses the associated emotions. As the interest of youths has increased recently, the importance of traditional costume education has been growing. Therefore, this study aims to analyze the contents of Hanbok in the 2015 revised high school technology and home textbooks using content analysis techniques and text mining techniques. As a result of the study, first, the symbolic meaning and characteristics of Hanbok and the beauty of Hanbok were practiced in daily life, and the value was found through the excellence of Hanbok and the modernization of Hanbok was dealt with Second, most of the illustrations related to traditional costumes were presented in various ways, but there were some regrets due to lack of quantity and quality. Third, the words used to explain traditional costumes were used in the form of culture, excellence, tradition, modernity, harmony, succession, etc. except for the types of clothing. Therefore, the results and discussions derived from this study are expected to help the textbooks to be efficiently selected and used in the field of the front line school along with the correct understanding of traditional culture in the process of selecting traditional culture contents and illustrations.

Chemical properties of Watercress(Oenanthe javanica D.C.) Depend upon Cultivating Methods (재배방법이 다른 미나리의 성분 특성)

  • Lee, Hong-Yeol;Yoo, Maeng-Ja;Chung, Hee-Jong
    • Journal of the Korean Society of Food Culture
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    • v.16 no.3
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    • pp.235-242
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    • 2001
  • Chemical properties of the different parts of watercress(Oenanthe javanica D.C.) grown and harvested from the culture fields under different cultivating methods were studied. In proximate analyses of watercress in parts, moisture contained more in stem than in root or in leaf, but crude protein and crude lipid contents were lower in stem than in root or in leaf. Crude ash in root contained up to about double amount in leaf or stem. Major mineral elements detected in watercress were Fe, Mg, Ca, and K, and their contents in root was higher than those in stem or leaf. Especially, Fe in root was significantly higher than that in stem or in leaf. Total free sugar composed mainly with fructose and glucose, was the highest in watercress from Hwasoon and followed the watercress from Jeonju and Donggok in order. Free sugar content was highest in leaf and the lowest in root. Major water-soluble vitamins were vitamin C, thiamin and biotin and the content of vitamin C was higher than others. These vitamins contained more in leaf than in stem or root. Niacin contained 6.09 mg/100 g in leaf of watercress from Hwasoon, which was much higher than others, but it was not detected in stem of watercress from Jeonju. Organic acids detected were oxalic acid, citric acid and malic acid and other 12 organic acids were not detected. In fatty acid composition, there were significant differences among watercresses from different parts and different culture fields. Linoleic acid, linolenic acid and palmitic acid were major fatty acids contained in watercress and it took about 80% of the total content. Amino acid content in leaf was higher than that in root and in stem. Glutamic acid and proline were major amino acids in stem of watercress from Jeonju and in stem of watercresses from Hwasoon and Donggok, respectively. In leaves of all three watercresses glutamic acid content was the highest.

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