• Title/Summary/Keyword: culture condition

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Study on the performance analysis of long-term field test for protected horticulture heating system using solar thermal energy (태양열 시설원예 난방시스템 장기실증 성능분석 연구)

  • Lee Sang-Nam;Kang Yong-Heack;Yu Chang-Kyun;Kim Jin-Soo
    • New & Renewable Energy
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    • v.1 no.2 s.2
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    • pp.53-59
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    • 2005
  • Objective of the research is to demonstrate solar thermal space and ground heating system which is integrated to a green-house culture facility for reducing healing cost, Increasing the value of product by environment control, and developing advanced culture technology by deploying solar thermal system. Field test for the demonstration was carried out in horticulture complex In Jeju Island. Medium scale solar hot water system was installed in a ground heating culture facility. Reliability and economic aspect of the system which was operated complementary with thermal storage and solar hot water generation were analyzed by investigating collector efficiency, operation performance, and control features. Short term day test on element performance and Long term test of the whole system were carried out. Optimum operating condition and its characteristics were closely Investigated by changing the control condition based on the temperature difference which Is the most important operating parameter For establishing more reliable and optimal design data regarding system scale and operation condition, continuous operation and monitoring on the system need to be further carried out. However, It is expected that, in high-insolation areas where large-scale ground storage is adaptable, solar system demonstrated in the research could be economically competitive and promisingly disseminate over various application areas.

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Candida magnoliae SR101에 의한 Erythritol의 생산에서 산업용 질소원의 선정 및 최척화

  • Park, Seon-Yeong;Seo, Jin-Ho;Yu, Yeon-U
    • 한국생물공학회:학술대회논문집
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    • 2001.11a
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    • pp.351-354
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    • 2001
  • In this experiment, we tested various nitrogen sources and then culture condition was optimized for industrial applications. The batch culture of Candida magnoliae SR101 grown in a defined medium supplemented with light steep water (LSW) as a sole nitrogen source showed a relatively high yield of erythritol production (53%), which was slightly higher than that using yeast extract as a nitrogen source, while the productivity and cell mass were maintained at similar levels. For the optimization of culture condition, the batch culture was performed. When the concentration of LSW was 65 mL/L in the defined medium containing 250 g/L of glucose, the concentration, yield and productivity of erythritol were 110 g/L, 44%, and 0.66 g/L-hr, respectively. The high yield and comparable productivity obtained with a cheap nitrogen source could be expected as a basis for the mass production of erythritol in the industrial scale.

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Asymbiotic seed germination and in vitro propagation condition in Calanthe discolor Lindl.

  • Kwon, Huyk Joon;Shin, So Lim;Bae, Kee Hwa;Kim, Soo-Young
    • Journal of Species Research
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    • v.6 no.1
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    • pp.68-75
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    • 2017
  • We investigated the effects of sodium hypochlorite (NaOCl) and culture medium on embryo swelling and germination of Calanthe discolor Lindl., and established a method for determining the swelling and protocorm formation of C. discolor seeds via in vitro examination of immature seeds. Treatment of immature seeds with NaOCl greatly enhanced the extent of embryo swelling and protocorm formation of immature zygote embryos compared to seeds without NaOCl treatment. The effects of the culture media were also evaluated with regard to embryo swelling and protocorm formation of in vitro cultured seeds with and without NaOCl treatment. Additionally, the effects of white fluorescent light and red and blue LEDs lights on seedling growth in in vitro culture were examined. The most suitable condition for seedling growth after 12 weeks of culture was the red LEDs light with POM medium. These results show effective asymbiotic germination and growth of C. discolor.

P(3HB) Accumulation in Alcaligenes eutrophus H16(ATCC 17699) under Nutrient-Rich Condition and Its Induced Production from Saccharides and Their Derivatives

  • Song, Jae-Jun;Shin, Yong-Chul
    • Journal of Microbiology and Biotechnology
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    • v.3 no.2
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    • pp.115-122
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    • 1993
  • Poly(3-hydroxybutyrate)(P(3HB)) accumulation under nutrient-rich condition with various amounts of $(NH_4)_2 SO_4$ was systematically investigated. The results of the electron-microscopy and the solvent extraction showed that the P(3HB) accumulation is unavoidable even under nutrient-rich condition. This indicates that in a two-step culture of Alcaligenes eutrophus H16, the researches should be careful in interpreting the data of polyhydroxyalkanoates(PHAs) accumulation in terms of the carbon-source fed in the second step because the two-step culture product contains the P(3HB) produced under nutrient-rich condition. The polyester production capability in a two-step batch culture of A. eutrophus H16(ATCC 17699) was also investigated using various saccharides and their derivatives such as glucose, fructose, gluconic acid, glucaric acid, sorbitol, lactose, galactose, and mannose. The polyesters synthesized were characterized by 500 MHz$^{1}H-NMR$ spectroscopy, intrinsic viscosity$[\eta]$ measurement in chloroform and differential scanning calorimetry(DSC). 500 MHz $^{1}H-NMR$ analysis showed that all polyesters synthesized generally contained 1~2 mol% of 3HV. Another finding is that the glucose utilization can be increased by changing the autoclaving procedure of the substrate to enhance the P(3HB) production yield up to 46 wt% of P(3HB) in dry cells.

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Effect of Phase Separation on Anaerobic Degradation of Phenol (페놀의 혐기성분해에 대한 상분리의 영향)

  • Park, Ju-Seok;Shin, Hang-Sik;Bae, Byung-Uk
    • Journal of Korean Society of Water and Wastewater
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    • v.8 no.1
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    • pp.27-33
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    • 1994
  • With the rapid industrialization, an ever-increasing quantity and kind of new organic compounds pose environmental problems due to their toxicity and physiological effect. However, research on the biodegradation of these compounds under anaerobic condition is very limited inspite of its efficiency and economical advantage. In this research, the pH effect on the ring cleavage of phenol under anaerobic condition was investigated, and the theory of phase separation was applied to the degradation of phenol for investigating the role of acidogenic bacteria. Results, obtained from biochemical methane potential(BMP) assay for 15.5 days of incubation, showed that acidic condition was more desirable for phenol degradation than alkaline condition. By both unacclimated methanogenic granular sludge and two mixed cultures, phenol was completely removed within six weeks of incubation with a gas conversion rate of over 86% of theoretical one. However, phenol was not degraded by unacclimated acidogenic culture, and thus it is considered as a syntrophic substrate. In case of phase separated biochemical methane potential(PSBMP) assay, in which acidogenic and methanogenic culture were seeded separately and consecutively, those that had been subjected to normal acidogens for 3 to 4 weeks showed higher gas production than those seeded with sterile or frozen culture.

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Optimization of in Vitro Cultivation of Inonotus Obliquus

  • Cho, Nam-Seok;Shin, Yu-Soo
    • Journal of the Korean Wood Science and Technology
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    • v.33 no.5 s.133
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    • pp.92-98
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    • 2005
  • This study was performed to get the basic information concerned to the optimum culture condition of Inonotus obliquus. Several solid media, PDA, MEA and Czapek-Dox, and three liquid media were adopted for the in vitro cultivation. Some main features of the fungal morphological characteristics under cultivation conditions were observed and described. Preliminary results showed that appearance of the mycelial mat, hyphal size and substrate pigmentation differed according to the media. The PDA medium was the most favorable substrate for the growth on solid culture, followed by MEA and Czapek-Dox media. Concerned to the addition of amino acids, 5 amino acids, such as alanine, alginine, isoleucine, leucine and threonine, enhanced to the mycelial growth. Isoleucine was shown the best fungal growth. An important morphological hyphal structure for the fungus, the setae, was found in abundance and diverse its shape and size. In liquid culture, fresh potato broth was the best growth stimulant of the fungus, followed by Malt extract and potato broth. Addition of yeast extract to the liquid media had improved the biomass, but not laccase production.

Isolation and Culture Characteristics of Strains for Color Removal of Reactive Dyes (반응성 염료의 색도제거를 위한 균주의 분리 및 성장 특성)

  • Kim, Jeong-Mog;Han, Myung-Ho;Lim, Hak-Sang
    • Journal of Korean Society of Water and Wastewater
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    • v.12 no.4
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    • pp.78-85
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    • 1998
  • Strains degrading and decolorizing reactive dyes, Procion blue HEGN and Procion red HE7B were isolated from water system, are named as RBK1 and RRK, the growth characteristics of which were investigated. Decolorization efficiencies after 42 hrs in batch culture were 95% and 77%, respectively. and the optimal culture condition of temperature and pH were $30^{\circ}C$, 7.0. Decolorization efficiencies in condition of aerobic shaking culture by strains RBK1 and RRK conspicuously increased, and culture by strain RBK1 was found as 95% after 42 hrs, while standing culture was 64%, Optimum nitrogen source was peptone, and It was found that decolorization efficiencies by strains RBK1 and RRK increased up to 4,000mg/l of peptone concentration as nitrogen source, but peptone concentration did' nt influence the decolorization efficiency in above 4,000mg/l. When the concentration of dyes were more than 800 mg/l and 400 mg/l respectively, the strains RBK1 and RRK, which degrade Procion bule HEGN and Procion red HE7B, showed a sharply decreased decolorization efficiencies; then the specific growth rate were $0.25hr^{-1}$ and $0.09hr^{-1}$.

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Irrigation System Based on Wireless Internet (무선 인터넷 기반의 급액관리 시스템)

  • Cho, Tae-Kyung
    • The Transactions of the Korean Institute of Electrical Engineers P
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    • v.58 no.4
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    • pp.422-426
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    • 2009
  • The key point on water culture is the precise irrigation control according to the growth condition of growing plant. And most existing irrigation controllers are operated independently. So, the manager must be resided at hydroponic farm. In this paper, we design the irrigation control system based on wireless Internet that can be providing various control functions according to the growth condition of growing plant by using the cellular phone.