• Title/Summary/Keyword: cultivation techniques

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An Overview of Different Techniques on the Microbial Community Structure, and Functional Diversity of Plant Growth Promoting Bacteria

  • Kim, Kiyoon;Islam, Rashedul;Benson, Abitha;Joe, Manoharan Melvin;Denver, Walitang;Chanratan, Mak;Chatterjee, Poulami;Kang, Yeongyeong;Sa, Tongmin
    • Korean Journal of Soil Science and Fertilizer
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    • v.49 no.2
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    • pp.144-156
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    • 2016
  • Soil is a dynamic biological system, in which it is difficult to determine the composition of microbial communities. Knowledge of microbial diversity and function in soils are limited because of the taxonomic and methodological limitations associated with studying the organisms. In this review, approaches to measure microbial diversity in soil were discussed. Research on soil microbes can be categorized as structural diversity, functional diversity and genetic diversity studies, and these include cultivation based and cultivation independent methods. Cultivation independent technique to evaluate soil structural diversity include different techniques such as Phospholipid Fatty Acids (PLFA) and Fatty Acid Methyl Ester (FAME) analysis. Carbon source utilization pattern of soil microorganisms by Community Level Physiological Profiling (CLPP), catabolic responses by Substrate Induced Respiration technique (SIR) and soil microbial enzyme activities are discussed. Genetic diversity of soil microorganisms using molecular techniques such as 16S rDNA analysis Denaturing Gradient Gel Electrophoresis (DGGE) / Temperature Gradient Gel Electrophoresis (TGGE), Terminal Restriction Fragment Length Polymorphism (T-RFLP), Single Strand Conformation Polymorphism (SSCP), Restriction Fragment Length Polymorphism (RFLP) / Amplified Ribosomal DNA Restriction Analysis (ARDRA) and Ribosomal Intergenic Spacer Analysis (RISA) are also discussed. The chapter ends with a final conclusion on the advantages and disadvantages of different techniques and advances in molecular techniques to study the soil microbial diversity.

History of Mushroom Industry in Korea (한국 버섯산업의 발전사)

  • You, Chang-Hyun
    • Journal of Mushroom
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    • v.1 no.1
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    • pp.1-8
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    • 2003
  • Mushroom cultivation in Korea was launched in the early 1960's. At that time Korean government started a program for increasing cultivation of button mushroom (Agaricus bisporus) and oak mushroom (Lentinula edodes) to export agricultural products and to increase farm income. National research institutes under Rural Development Administration, Korea Forest Service, etc. play a leading role in mushroom industry as follows : Development and spread of genetically superior commercial strains, good spawns, and cultivation techniques. Training and field advice to lead farmers for mass production of high quality mushrooms. Political support of facilities and establishments for mushroom cultivation. Several mushrooms including oak mushroom, button mushroom, oyster mushroom, winter mushroom, Ganoderma, P. eryngii, etc. have been popularized for their cultivation techniques and produced in large quantities in the farm. According to a recent statistics, mushrooms have been grown by about 20,000 farm households, in Korea and the gross production of fresh mushrooms is estimated about 170,000 M/T. The gross production of oyster mushroom is the highest followed by winter mushroom, oak mushroom and button mushroom. The gross amount of mushroom production stands over 700 billion won. Thus, mushroom industry goes to the most important cash crop to be produced yearly.

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Economy analysis of cost required for organic rice cultivation in two cultivation techniques

  • Kwon, Young-Rip;Choi, In-Young;Lee, Yong-Mahn;Choi, Dong-Chil;Kim, Yong-Ki
    • Korean Journal of Organic Agriculture
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    • v.19 no.spc
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    • pp.92-95
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    • 2011
  • The initial investment of rice cultivation by mechanical technique was high. This was only due to the cost of planting machine. We analysed the overall cost (without machine cost) and benefits of the two methods, it manifested that the mechanical method is very much beneficial over traditional rice cultivation technique. We observed that the requirement of soil, irrigation water, number of seeds etc. decreased in mechanical cultivation technique while net income increase by 13.07% with 80% government subsidy. Furthermore, the initial rooting was quicker, growth and development of pot raised seedlings was better than the traditional technique. Hence, this technique can give better return to the farmers with the help of government subsidy.

Impact of Different Environmental Conditions and Management Techniques on Productivity of Grassland in Central and Southern Area of Korea (중부 및 남부지역에서 초지환경과 관리기술의 차이가 초지의 생산성에 미치는 영향)

  • Choi, Gi Jun;Jung, Jeong Sung;Choi, Ki Choon;Hwang, Tae Young;Kim, Ji Hye;Kim, Won Ho;Lee, Eun Ja;Sung, Kyung Il;Lee, Ki-Won
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.40 no.2
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    • pp.87-97
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    • 2020
  • This experiment was carried out to study the effects of different environmental conditions and cultivation techniques on productivity of grasslands in central and southern area of Korea on 2017 and 2018. Average dry matter yield of grasslands at 10 actual production sites was 7,496 kg/ha. that was ranged from 4,652 to 13,292 kg/ha with least significant difference(LSD) of 1,577kg/ha between grasslands (p<0.05) on 2017. Average dry matter yield of grasslands at 10 actual production sites was 7,914 kg/ha. that was ranged from 3,927 to 12,372 kg/ha with LSD of 1,577kg/ha between grasslands(p<0.05) on 2018. Dry matter(DM) yield of grasslands have positive correlation with soil fertility (p<0.01) but not correlated with rainfall and air temperature among cultivation environments. And also DM yield of grasslands have positive correlation with grassland management techniques(p<0.01). These results suggest that practices of grassland management techniques and improvement of soil fertility are more important than cultivation environments by climate change for increasing the DM yield of grassland in central and southern area of Korea.

Cultivation and Characteristic Methods for DUS Test New Varieties of Actinidia arguta -Based on the Process of Writing the Manual- (다래(Actinidia arguta) 신품종 심사를 위한 재배 및 특성조사방법 -매뉴얼 작성과정을 기준으로-)

  • Mi-Yun An;Ro-Young Lee;Je-Min Park;Byeong-Hoon Yang;Ki-Yoon Kim
    • Korean Journal of Environmental Agriculture
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    • v.42 no.4
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    • pp.450-456
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    • 2023
  • The National Forest Seed and Variety Center aims to write a manual for cultivation and characteristic inspection of new seed varieties in Korea, serving dual purposes. The first is the enhancement of the test guidelines, particularly the distinctness, uniformity and stability (DUS) test, for new seed variety, where research and examination are conducted based on the test guidelines. However, if the crop-specific test guidelines are initially reorganized, the details are unclear. Therefore, it seeks to maintain a written record to prevent confusion when the person in charge is changed. The second is to encourage the development of new varieties. By incorporating general characteristics and cultivation techniques in the manual, it seeks to encourage breeders to develop new varieties. Additionally, it serves as an important chronological record of the creation process, thereby helping future manual authors. This article introduces the current status of new plant variety protection and outlines the significance of creating a manual for cultivation and characterization of Actinidia arguta for screening new varieties.

Introduction of the representative mushroom cultivars and groundbreaking cultivation techniques in Korea

  • Jang, Kab-Yeul;Oh, Youn-Lee;Oh, Minji;Woo, Sung-I;Shin, Pyung-Gyun;Im, Ji-hoow;Kong, Won-Sik
    • Journal of Mushroom
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    • v.14 no.4
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    • pp.136-141
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    • 2016
  • The production scale of mushrooms in Korea is approximately 600 billion won, which is 1.6% of Korea's gross agricultural output. In Korea, ca. 190,000 tons of mushrooms are harvested annually. Although the numbers of mushroom farms and cultivators are constantly decreasing, total mushroom yields are increasing owing to large-scale cultivation facilities and automation. The recent expansion of the well-being trend has caused an increase in mushroom consumption in Korea: the annual per capita mushroom was 3.9 kg ('13), whichis a little higher than that in Europe. Thus, mushroom export, mainly Flammulina velutipes and Pleurotus ostreatus, has increased since the mid-2000s. Recently, however, it is slightly reduced. Nevertheless, Vietnam, Hong Kong, the United States, and the Netherlands continue to export mushrooms, and Korea has increased its export to Australia, Canada, Southeast Asia, etc. Canned Agaricus bisporus, the first export of the Korean mushroom industry, reached it speak sales in 1977-1978. When Korea initiated trade with China in 1980, the international prices of mushrooms fell sharply, leading to shrinkage of the domestic markets. Spurred by the high demand to develop substitute goods for A. bisporus, the oyster mushroom (P. ostreatus) gained attention since it seemed to suit the taste of Korean consumers. Although the log cultivation technique for oyster mushroom was developed in the early 1970s, it required a great deal of labor. Thus, we developed the shelf cultivation technique, which is easier to manage and allows for mass production. In this technique, the growing shelf is made mafrom fermented rice straw, whichis the only P. ostreatus medium in the world and isused only in South Korea. After then, the use of cotton wastes as an additional material of medium, the productivity. Currently, we are developing a standard cultivation technique and environmental control system that can stably produce mushrooms throughout the year. The increase of oyster mushroom production may boostthe domestic market and contribute to industrial development. In addition, oyster mushroom production technology played a role in forming the basis for the development of bottle cultivation, which made mass production. In particular, bottle cultivation using liquid spawn could allow for the export of F. velutipes and Pleurotus eryngii. In addition, the white varieties of F. velutipes were second developed in the world after Japan. We also developed the new A. bisporus cultivar 'Saeah', which is easy to grow in Korea. In hopes to advance the mushroom industry, we will continue to develop cultivars with international competitive power and to improve cultivation techniques.

Isolation of Microorganisms for Biotechnological Application

  • Franco, Christopher-M.M.;Mcclure, Nicholas-C.
    • Journal of Microbiology and Biotechnology
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    • v.8 no.2
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    • pp.101-110
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    • 1998
  • The extent of biological diversity being revealed by molecular techniques accentuates the need to develop methods to isolate and culture the large numbers of microorganisms that remain to be studied. The discovery and characterization of novel microorganisms will provide information useful in understanding microbial ecosystems and have the potential to lead to new products for the biotechnology industry. In this review, the use of innovative techniques and exploration of unusual ecosystems, that have begun to address the challenge of isolating the "uncultured" members of the microbial population, are examined.

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Detection Techniques for Greenhouse Area on Paddy Fields Using Landsat TM Images (Landsat TM 영상을 이용한 논지역 내 비닐하우스 면적 추정)

  • Jung In-Kyun;Park Geun-Ae;Jang Cheol-Hee;Kim Seong-Joon
    • KCID journal
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    • v.8 no.2
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    • pp.45-54
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    • 2001
  • A plenty of wastes by greenhouse cultivation affect soil and water pollution much more than those by rice cultivation in paddy field. The greenhouse on paddy field has been increased dramatically, however their physical information such as the location an

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Effects of Organic Fertilizers and Green Manure Crops on Growth Responses and Yields of Astragalus membranaceus Bunge (황기의 녹비작물 작부체계 활용 유기재배 시 생장반응과 수량에 관한 연구)

  • Song, Beom-Heon;Lee, Kyung-A;Chang, Yoon-Kee;Kim, Young-Gook;Ahn, Tae-Jin;Ahn, Young-Seob;Park, Chung-Beom
    • Korean Journal of Medicinal Crop Science
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    • v.19 no.2
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    • pp.83-89
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    • 2011
  • This studies were conducted to investigate the growth responses and yields of Astragalus membranaceus Bunge which were grown with organic cultivation using two organic fertilizers and two green manure crops of hairy vetch and rye in 2008 and 2009. The lengths of stems and roots were not clearly different between organic and conventional cultivations. The root diameters and the branch numbers were slightly thicker and more in all treatments of organic cultivation than that of conventional cultivation in 2008, although they were not significantly different between two cultivating methods in 2009. The dry weights of shoot and root were heavier on organic cultivation than those on conventional cultivation. The T/R ratios were commonly higher in organic culture, showing more clearly in 2008 than 2009. The yields were generally higher in organic cultivation than those in conventional cultivation in 2008 although the effect did not clear on yield in 2009. The organic cultivation using of organic fertilizers and green manure crops would be new method to have safety and qualitative products. The techniques of organic cultivation for Astragalus membranaceus Bunge would be studied more on utilizing the natural organic resources.