• Mycobiology
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• v.35 no.2
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• pp.82-86
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• 2007

Ergone, a fungal metabolite derived from ergosterol, was previously isolated and identified from Polyporus umbellatus. Ergone is a major component of P. umbellatus known to have anti-aldosteronic diuretic effect and also displays cytotoxic activities. Most of mushroom's fruit bodies used for test contained less than 10 ${\mu}g/g$ of ergone. But P. umbellatus have larger amount of ergone than any other mushrooms. In order to improve the ergone production from the submerged culture of P. umbellatus, several factors including medium composition, culture conditions (temperature and pH) and different combinations of co-cultivation with various mycelia were studied. Among various carbon sources examined, starch proved to be most effective for the production of mycelia. The optimum pH and temperature for a flask culture of P. umbellatus mycelia were found to be 4.5 and $25^{\circ}C$, respectively. Under the optimized culture conditions, both the ergone production (86.9 ${\mu}g/g$) and mycelial growth (3.5 g/l) increased when P. umbellatus was cultured with Armillariella mellea. When the optimized conditions were applied, both mycelium and ergone production were significantly enhanced.

• Journal of Pharmacopuncture
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• v.20 no.4
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• pp.274-279
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• 2017

Objective: SanYangSam and SanYangSanSam are traditional Korea-medical herbs that are grown from Panax ginseng C.A. Meyer. In our previous studies, we found that the functional compounds in SanYangSam and SanYangSanSam were different and depended on the type and the cultivation environment of ginseng. This study aimed to profile the functional constituents in SanYangSam and SanYangSanSam. Methods: To profile the functional aspects of the many compounds that have therapeutic activities in SanYangSam and SanYangSanSam extracts, we used liquid chromatography tandem mass spectrometry and quadrupole orthogonal acceleration time-of-flight mass spectrometry. Results: A total of four major compounds were detected; two of which were the natural flavonoids kaempferol and quercetin. Among others, two polyacetylene compounds, including panaxydol and panaxynol, were detected. Conclusion: In this study, we found that panaxydol, one of the polyacetylene constituents of ginseng, is a candidate anti-cancer agent in SanYangSam and SanYangSanSam pharmacopuncture. In addition, we found that the panaxydol levels in the SanYangSanSam extract were over 30 times those in the SanYangSam extract.

• Journal of Microbiology and Biotechnology
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• v.22 no.12
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• pp.1605-1612
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• 2012

The present study concerned the identification and characterization of a novel bacterial strain isolated from entomopathogenic nematodes collected from different regions in Korea. The bacterial isolate M1021 was Gramnegative, bioluminescent, and produced red colonies on MacConkey agar medium. A rod-shaped structure was confirmed by the electron micrograph. Fatty acid composition was analyzed by using the Sherlock MIDI system. The identification was further supported by 16S rDNA sequence analysis, which revealed 96-99% sequence homology with strains of Photorhabdus temperata. The location of the isolated strain of P. temperata in the phylogenetic tree was confirmed and it was named P. temperata M1021. P. temperata M1021 exhibited catalase, protease, and lipase activities when grown on appropriate media supplemented with respective substrates. The culture of P. temperata M1021 exhibited insecticidal activity against the larvae of Galleria mellonella and the activity was the highest after 3-4 days of cultivation with agitating at $28^{\circ}C$ under 220 rpm. Antibacterial activity was also observed against Salmonella Typhimurium KCTC 1926 and Micrococcus luteus KACC 10488.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.19 no.7
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• pp.1705-1710
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• 2015

There have been lots of researches and developments for smart agriculture that enables higher efficiency and productivity by applying ICT into the agriculture field recently. However, there still remain many difficulties in purchasing foreign equipments or overseas expansion due to lack of international standards that are internationally approved. Such necessity leads to the international standardization activities driven by domestic experts especially in ITU-T SG13. This paper presents several smart agriculture services based on the service approach that can effectively describe use cases. The proposed architecture of service provision was applied to the document that has been consented as the ITU-T Recommendation Y.2238.

• Journal of Marine Bioscience and Biotechnology
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• v.5 no.4
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• pp.15-25
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• 2011

Marine sponges are a rich source of highly diversified bioactive compounds. These medicinally valuable molecules represent extreme physiological and ecological functions in sponges, more presumably involving in the resistance to the feeding by other marine organisms like fish and fouling by barnacles, bacteria, fungi, etc. This feature of attaining resistance made sponges as successful poriferans that possess an impressive array of biological properties ranging from antimicrobial to anticancerous activities. The diversified bioactive principle of sponges might be due to their spacio-temporal distribution and although, the gateway for exploiting the sponges for isolating these distinct, potential molecules is open, suitable technical and methodological approaches are yet to be implemented in order to bring the sponges as successful pharmaceutical leads in the field of marine biotechnology. Despite of the identified difficulties of marine sponge research from past few decades, one should concentrate not only on the basic and applied technical/methodological considerations, but also on the novel strategies like in vitro sponge cell, fragment and whole sponge culture; sponge symbiont cell culture; in situ and ex situ sponge cultivation; and sponge bioreactors and metagenomic approaches, for the successful exploitation of marine sponges towards the novelty in sponge biotechnology. The present review narrates the pros and cons of the nowadays-marine sponge research by focusing on the suggestive ecobiotechnological approaches, based on the latest studies for feasible ecological exploitation and biotechnological application of sponges from the sea.

• Korean Journal of Remote Sensing
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• v.26 no.3
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• pp.287-295
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• 2010

Water level of the Tonle Sap is largely influenced by the Mekong River. During the wet season, the lacustrine landform and vegetated areas are covered with water. Change detection in this area provides information required for human activities and sustainable development around the Tonle Sap. In order to detect the changes in the Tonle Sap floodplain, fifteen ALOS-PALSAR L-band data acquired from January 2007 to January 2009 and examined in this study. Since L-band is able to penetrate into vegetation cover, it enables us to study the changes according to water level of floodplain developed in the rainforest. Four types of images were constructed and studied include 1) ratio images, 2) correlation coefficient images, 3) texture feature ratio images and 4) multi-color composite images. Change images (in each 46 day interval) extracted from the ratio images, coherence images and texture feature ratio images were formed for detecting land cover change. Two RGB images are also obtained by compositing three images acquired in the early, in the middle and at the end of the rainy season in 2007 and 2008. Combination of the methods results that the change images present the relationship between vegetation and water level, leaf fall forest as well as cultivation and harvest crop.

• International Journal of Advanced Culture Technology
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• v.7 no.2
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• pp.144-150
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• 2019

Recently, an increasing number of urban farmers are growing fresh vegetables and fruits themselves using urban garden or weekend farms. In other words, this is called urban agriculture. After the Industrial Revolution in the 18th century, the agricultural society changed from an agricultural society to an industrial society, and the population began to flock to the cities. With the continued increase of urban population, countries with abundant capital were able to control the distribution structure of food supplies and trade agreements among countries. Since energy consumption and carbon dioxide emissions caused by agricultural product movements have emerged as the main culprits of global warming, and our table is threatened by safety due to food supply that has no genetic modification or knowledge of the cultivation process, urban agriculture is already taking hold in the West. In other words, as agriculture met cities, its role grew. Each region actively conducts agricultural activities for raising poultry as well as growing vegetables and fruits by using flower beds of detached houses in the city center, rooftops of high and low buildings, or school playgrounds and small tributaries of land. The purpose of this study is to analyze and understand the significance and type of urban agriculture and to examine the cases of domestic and foreign urban agriculture based on this and to seek the developing direction of urban agriculture, which is gradually increasing. Tired of growing competition and rapid change, urbanites are seeking health and relaxation and are planning to present development measures for urban farming and conduct follow-up research to ensure safe food.

• Journal of Microbiology and Biotechnology
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• v.28 no.12
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• pp.2064-2070
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• 2018

Pseudomonas tolaasii 6264 is a representative strain that causes bacterial blotch disease on the cultivated oyster mushroom, Pleurotus ostreatus. Bacteriophages are able to sterilize the pathogenic P. tolaasii strains, and therefore, they can be applied in creating disease-free mushroom cultivation farms, through a method known as "phage therapy". For successful phage therapy, the characterization of phage-resistant strains is necessary, since they are frequently induced from the original pathogenic bacteria in the presence of phages. When 10 different phages were incubated with P. tolaasii 6264, their corresponding phage-resistant strains were obtained. In this study, changes in pathogenic, genetic, and biochemical characteristics as well as the acquired phage resistance of these strains were investigated. In the phylogenetic analyses, all phage-resistant strains were identical to the original parent strain based on the sequence comparison of 16S rRNA genes. When various phage-resistant strains were examined by three different methods, pitting test, white line test, and hemolytic activity, they were divided into three groups: strains showing all positive results in three tests, two positive in the first two tests, and all negative. Nevertheless, all phage-resistant strains showed that their pathogenic activities were reduced or completely lost.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• v.3 no.2
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• pp.73-76
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• 2022

Most of ants seem to be beneficial, whereas some of them are annoying or harmful. Among harmful creatures red imported fire ants have been notorious for not only destroying farm plants and domestic animals but also biting and killing human beings. The red imported fire ants, Solenopsis invicta Buren and Solenopsis richiteri Forel, will feed on the buds and fruits of numerous crop plants. Large nests located in fields interfere with and damage equipment during cultivation and harvesting. They respond rapidly and aggressively to disturbances, and ant attacks inhibit field worker activities. A single fire can sting its target repeatedly. Young and newborn animals as well as humans are especially susceptible to the stings' venom. These pests can damage the environment by displacing native ant species and reducing food sources for wildlife. In Korea Solenopsis invicta Buren were found for the first time in Busan Gamman port in 2017. Then they were found in Incheon port, Pyongtaek port, North Daegu, Ansan in 2018 and Gwangyang in 2021. Once they invaded and occupied the terrestrial area, we have no proper solution to prevent them. I can assume that they have been successfully enlarging their colonies toward inland since they can move freely by flight. Therefore, I strongly suggest that we need to study ants in order not only to control harmful ants properly but also to preserve and use beneficial ants in this country.

• Journal of Industrial Convergence
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• v.21 no.1
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• pp.147-157
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• 2023

In this study, we approached from the perspective of AI convergence education in elementary, middle and high schools to understand AI convergence education. We examined what capabilities AI convergence education ultimately seeks to pursue, and analyzed various examples of AI convergence education in three dimensions: core curriculum, convergence model, AI learning elements and learning activities. In addition, factors to be considered in order for AI convergence education to be actively carried out include the cultivation of AI convergence education capabilities of teachers, the development and dissemination of AI teaching and learning methods and teaching and learning models, and evaluation methods for AI convergence education.