• Journal of The Korean Society of Grassland and Forage Science
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• v.18 no.1
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• pp.35-42
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• 1998

Eleven Italian ryegrass cultivars were examined for their genetic polymorphisms and phylogenetic relationships using randomly amplified polymorphic DNA (RAPD) markers. In RAPD analysis of 34 random primers, 96 of total 162 bands obtained from 16 primers were polymorphic and sizes of polymorphic band ranged between 0.5 and 1.5kb. Number of bands amplified per primer was varied from 3 to 16 and average number was 14.8. Phylogenetic relationship among cultivars based on the RAPD analysis was examined using UPGMA computer program. In pairwise genetic similarity test of 11 Italian ryegrass cultivars, Grazer and Orlando showed highest coefficient of genetic similarity as 0.740, whereas Marshall and Orlando was lowest as 0.438. Eleven Italian ryegrass cultivars were grouped into 3 major clusters and genetic distance of clusters ranged between 0.567 and 0.646, indicating low level of genetic variation.

• Journal of Sericultural and Entomological Science
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• v.43 no.2
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• pp.88-92
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• 2001

The crossabilities among 3 wild mulberry species and 3 cultivars growing in the Korean Peninsula had been studied. The result of the crossabilities of Moms tiliaefolia with Moms bombycis and 3 cultivars were possible, but with Morus mongolica was impossible. Those of Moms mongolica with Moms bombycis, Morus tiliaefolia and 2 cultivars except Daeryukppong were possible too. And those of Morus bombycis with Moms titiaefolia, Moms mongolica were impossible, but with 3 cultivars were possible moderately.

• Mycobiology
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• v.29 no.2
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• pp.85-89
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• 2001

URP primers of 20 mer derived from repetitive sequence of rice were used to assess genetic variation of oyster mushroom consisting of 10 cultivars of Pleurotus ostreatus, two cultivars of P. florida and two cultivars of P. sajor-caju which were registered in Korea. URP2F and URP38F primers produced cultivar-specific PCR polymorphic bands in the Pleurotus species. UPGMA cluster analysis using the URP-PCR data showed that 14 Pleurotus cultivars are genetically clustered into large three groups. The URP-PCR data provided important information for more efficient breeding strategies of Pleurotus cultivars.

• Journal of the Korean Society of Tobacco Science
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• v.30 no.2
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• pp.85-93
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• 2008

This report describes a set of seven informative single-nucleotide polymorphisms (SNPs) and one insertion-deletion (INDEL) distributed over 24 cultivars that can be used for tobacco (Nicotiana tabacum L.) cultivar identification. We analyzed 163,000 genomic DNA sequences downloaded from Tobacco Genome Initiative database and assembled 31,370 contigs and 60,000 singletons. Using relatively long contigs, we designed primer sets for PCR amplification. We amplified 61 loci from 24 cultivars and sequenced the PCR products. We found seven significant SNPs and one INDEL among the sequences and we classified the 24 cultivars into 10 groups. SNP frequency of tobacco, 1/8,380 bp, was very low in comparison with those of other plant species, between 1/46 bp and 1/336 bp. For exact identification of tobacco cultivars, many more SNP markers should be developed. This study is the first attempt to identify tobacco cultivars using SNP markers.

• Asian Journal of Turfgrass Science
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• v.5 no.1
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• pp.11-22
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• 1991

This experiment was executed to investigate the possibility of the application of taxonomic method through the isoelectric focusing with polyacrylamide gel and sodium dodecyl sulfate-polyacrylamide gel electrophoresis with seeds in the identification of turfgrasses. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to investigate the pattern of seed proteins which were extracted from 18 cultivars of cool season turfgrass and 4 cultivars of warm season turfgrass. The isoelectric focusing with polyacrylarnide gel was used to investigate the activity of the three isozymes of esterase, peroxidase and phosphoglucose isomerase which were extracted from 18 cultivars of cool season turfgrass and 4 cultivars of warm season turfgrass. The results were summarized as follows. 1. The difference of the patterns of seed proteins was observed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The identification of intra-genus was easily detected. 2. The three isozymes of esterase, peroxidase and phosphoglucose isomerase were investigated through isoelectric focusing with polyacrylamide gel. As a result, esterase was most effective among three isozymes in the identification of turfgrass cultivars 3. In the past cultivar identification was primarily based on visual morphological characters, but there was a lot of difficulty. If we should use electrophoresis, we will be able to identifvturfgrass cultivars more effectively.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.63-63
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• 2018

Ethnobotanical knowledge on native plants is critical on the conservation and management of biological resources. We investigated traditional knowledge of ethnobotanical uses in the Mt. Gayasan National Park area. Interviews were carried out to 189 residents at 176 places, and verified species and usage information was categorized by taxonomic groups, usage, and used parts. The ethnobotanical species of the regions consisted of a total of 275 taxa in 91 families including 105 cultivars. Sunflower family (Asteraceae) is the most widely used family with 30 taxa (7 cultivars). Rose family (Rosaceae, 25 taxa with 11 cultivars), Bean family (Fabaceae, 15 taxa with 11 cultivars), Grass family (Poaceae, 15 taxa with 10 cultivars), and Lily family (Liliaceae, 14 taxa with 4 cultivars) followed. About 50 taxa belong to the Approved Species for Delivering Overseas designated by Korea Ministry of Environment, and six Korean endemic taxa have been traditionally valuable in the regions. Many cultivated species have been utilized for various purposes accompanied local and native plants. The main usage of the plants are edible (175 taxa) and medicinal (168 taxa). Leaves are most commonly used parts (105 taxa), followed by stems (93 taxa), fruits (73 taxa), roots (55 taxa), and wholes (54 taxa). The study does not only provides examples of traditional uses of native plants, but also facilitates conservation of natural resources and sustainable developments of ethnobotanical knowledge for the contemporary human society.

• Korean Journal of Agricultural Science
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• v.6 no.2
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• pp.119-124
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• 1979

In order to examine $SO_2$ injury to apple shoots, cutting shoots of five cultivars were exposed to 3 ppm $SO_2$ in vase life conditions. 1. The most sensitive cultivar to $SO_2$ was 'Rall's Genet', and 'Starking' was most tolerant among cultivars tested. 2. Ethylene production of shoots showed peak after 8 hours exposure, when visible injury was not appeared, and was decreased with increase in exposure time. 'Rall's Genet' showed slower decrease in ethylene production than other cultivars. 3. Chlorophyll a, b and a/b ratio were decreased in all cultivars after 40-hr exposures to 3 ppm $SO_2$ and greatly decreased in 'Rall's Genet' compared to other cultivars.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.320-320
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• 2017

Recently, the rice growing time was extended by the global warming. This study was conducted to investigate the possibility of rice double cropping system in southern Korea. The first transplanting was with six cultivars ('Kilala397', 'Baekilmi', 'Joun', 'Hanseol' and 'Jungmo1031') on April 15. All cultivars could be harvested at the end of July. Adaptable cultivars for the first cultivation were 'Kilala397', Baekilmi' and 'Joun'. The rice yields at the first cultivation was about 95% of local average yield. Although the yield was slightly less, the first cultivation was considered to have economic benefits, because of the high market price of rice. In the second transplanting was with five cultivars ('Manjong', 'Joun', 'Deabo', 'Jinok' and 'Kilala397') on April 15. All cultivars could be harvested in early November. Adaptable cultivars for the second cultivation were 'Jinok' and 'Kilala397'. The rice yields at the second cultivation was about below the 60% of local average yield, because there was the less growth than normal season cultivation. Consequently, southern Korea' annual rice double cropping system is considered to have no economic value yet. However, the research should be continued considering the temperature rise of global warming.

• The Plant Pathology Journal
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• v.16 no.5
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• pp.290-293
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• 2000

The main grape producing regions in Korea were surveyed for the occurrence and relative incidence of crown gall disease on grapevine. The results of the survey showed that the disease tended to affect tetraploid cultivars, which produce a large and sweet fruit but are very weak to cold weather. Incidence of crown gall disease was high on the tetraploid cultivars, Kyoho (Gerbong), Daebong, and Black Olympia while it was low on the diploid cultivars, Cambell Early and Sheridan. The disease incidence was very high on Anseong, Cheonan, and Chincheon, where the major growing areas of tetraploid cultivars and grapevines were burried in the winter to protect from freeze injury, whereas it was low in Yungdong, Kimcheon, and Nonsan. Crown gall disease did not increased with grapevine age on both Cambell Early and Kyoho. It remained low in Cambell Early, but high for all ages on Kyoho, Galls were found on the crown region and mid part of the trunk, but more galls were on small branches and canes on Kyoho grapevines. More than one third of ZKyoho grapevines inspected had galls on multiple locations on grapevines. On Kyoho, 56.3% of the galls covered more than 50% of the crown gall disease affects severely on the cultivar.

• The Plant Pathology Journal
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• v.27 no.2
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• pp.183-186
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• 2011

During the summer of 2005, specimens of soybean cultivars (Daepung, Daewon, Hwanggeum and Taegwang) showing frogeye leaf spot (FLS) were obtained from various areas in Korea. Eight isolates identified as Cercospora sojina were inoculated on the adaxial leaf surfaces of 63 Korean soybean cultivars; the disease responses to each isolate were evaluated 14 days and 21 days after inoculation. Based on the disease responses (resistant or susceptible) of the cultivars by the isolates, a set of cultivars (Anpeong, Bogwang, Cheongdu No. 1, Cheongja No. 3, Dachae, Daemang, Jangwon, Namhae, Sowon, Taegwang) were selected and inoculated with seven isolates for further testing pathogenic variance. Interestingly, 6 out of 7 tested C. sojina isolates revealed differential ability in infecting different soybean cultivars. This result may indicate the possibility of new race occurrence or pathogenic variation; this also presents evidence for prevalent FLS occurrence during humid and hot weather in Korea.